Silkworm BLAST Result against tribolium

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001148-TA|BGIBMGA001148-PA|IPR001092|Basic
helix-loop-helix dimerisation region bHLH, IPR011598|Helix-loop-helix
DNA-binding
         (239 letters)

Database: tribolium 
           317 sequences; 114,650 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY321476-1|AAQ23386.1|  253|Tribolium castaneum Ash protein.          109   2e-26
AY321476-2|AAQ23387.1|  257|Tribolium castaneum Ase protein.           69   2e-14
AJ536347-1|CAD60438.1|   40|Tribolium castaneum putative Ash pro...    32   0.003
U61151-1|AAB41306.1|   45|Tribolium castaneum TATH1 protein.           29   0.024
S73230-1|AAB30812.1|   35|Tribolium castaneum twist protein.           28   0.056
AJ829922-1|CAH25640.1|  193|Tribolium castaneum twist bHLH trans...    28   0.056
S73225-1|AAB30811.1|  327|Tribolium castaneum protein ( Triboliu...    24   1.2  

>AY321476-1|AAQ23386.1|  253|Tribolium castaneum Ash protein.
          Length = 253

 Score =  109 bits (261), Expect = 2e-26
 Identities = 79/210 (37%), Positives = 105/210 (50%), Gaps = 17/210 (8%)

Query: 38  IAPAPEKNYVTHDTPPNLHYRKKVHFRTNPYTGPQA--ASIARRNARERNRVKQVNDGFN 95
           +APAPE+  V      +L  ++K+ F   PY GPQ   AS+ARRNARERNRVKQVN+GF 
Sbjct: 50  LAPAPERTSVLVTNNSDLRCKRKIQFM--PY-GPQQQPASVARRNARERNRVKQVNNGFA 106

Query: 96  ALRRHLPASXXXXXX--XXXXXXXXXXXXXXXDTLRMVVEYIRYLQQLL-DESDAALGIT 152
            LR+H+PAS                       +TLR+ VEYIR L+Q++ D  +   G +
Sbjct: 107 TLRQHIPASVAAAFAPQGPSTGRGASKKLSKVETLRLAVEYIRSLKQMIEDHENDTTGNS 166

Query: 153 RDQENRENIPSNNSVQPMTSIDMDDGFFYGSGSPCSEKADSPAPSECS----SGVSSAYS 208
            +   +EN   NNS     S  +           CSE + SP PS  S    S  SS  +
Sbjct: 167 GEGSLQENRYYNNSPDQYQSYQI-----LLPTPTCSEASSSPTPSHSSESSFSAASSYTN 221

Query: 209 AVDRYEVTTQQQMGSMDEEELLDVISWWQQ 238
            +   E     +  S ++EELLD I  WQQ
Sbjct: 222 TIYHQENFENYEPKSPEDEELLDAIFSWQQ 251


>AY321476-2|AAQ23387.1|  257|Tribolium castaneum Ase protein.
          Length = 257

 Score = 69.3 bits (162), Expect = 2e-14
 Identities = 39/79 (49%), Positives = 46/79 (58%), Gaps = 4/79 (5%)

Query: 69  TGPQAASIARRNARERNRVKQVNDGFNALRRHLPASXXXXXXXXXXXXXXXXXXXXXDTL 128
           T PQ  ++ARRNARERNRVKQVN+GF  LR+H+P                       +TL
Sbjct: 47  TAPQPVAVARRNARERNRVKQVNNGFANLRQHIP--NFIAAAFESNSRGGNKKLSKVETL 104

Query: 129 RMVVEYIRYLQQL--LDES 145
           RM VEYIR L+ L  LDES
Sbjct: 105 RMAVEYIRSLEDLLALDES 123


>AJ536347-1|CAD60438.1|   40|Tribolium castaneum putative Ash
           protein protein.
          Length = 40

 Score = 32.3 bits (70), Expect = 0.003
 Identities = 15/21 (71%), Positives = 18/21 (85%), Gaps = 1/21 (4%)

Query: 82  RERNRVKQVNDGFNALRRHLP 102
           RERNRVK VN+GF  LR+H+P
Sbjct: 1   RERNRVK-VNNGFANLRQHIP 20


>U61151-1|AAB41306.1|   45|Tribolium castaneum TATH1 protein.
          Length = 45

 Score = 29.5 bits (63), Expect = 0.024
 Identities = 12/24 (50%), Positives = 17/24 (70%)

Query: 80  NARERNRVKQVNDGFNALRRHLPA 103
           NARER R+  +ND F+ LR  +P+
Sbjct: 3   NARERRRMNSLNDAFDRLRDVVPS 26


>S73230-1|AAB30812.1|   35|Tribolium castaneum twist protein.
          Length = 35

 Score = 28.3 bits (60), Expect = 0.056
 Identities = 10/23 (43%), Positives = 16/23 (69%)

Query: 80  NARERNRVKQVNDGFNALRRHLP 102
           N RER R + +N+ F +LR+ +P
Sbjct: 2   NVRERQRTQSLNEAFASLRKSIP 24


>AJ829922-1|CAH25640.1|  193|Tribolium castaneum twist bHLH
           transcription factor protein.
          Length = 193

 Score = 28.3 bits (60), Expect = 0.056
 Identities = 10/23 (43%), Positives = 16/23 (69%)

Query: 80  NARERNRVKQVNDGFNALRRHLP 102
           N RER R + +N+ F +LR+ +P
Sbjct: 102 NVRERQRTQSLNEAFASLRKSIP 124


>S73225-1|AAB30811.1|  327|Tribolium castaneum protein ( Tribolium
           castaneum homeodomainprotein mRNA, complete cds. ).
          Length = 327

 Score = 23.8 bits (49), Expect = 1.2
 Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 5/55 (9%)

Query: 159 ENIPSN-NSVQPMTSIDMDDGFFYGSGSPCSEKADSPAPSECSSGVSSAYSAVDR 212
           +NI S+ N++  +T+  +D G      SP S +  SP+ ++  S     Y+  DR
Sbjct: 43  KNIESHLNAISQITNSTLDPG----RKSPSSPRISSPSSTKSGSPGFLTYTKADR 93


  Database: tribolium
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 114,650
  Number of sequences in database:  317
  
Lambda     K      H
   0.312    0.127    0.364 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 51,988
Number of Sequences: 317
Number of extensions: 2205
Number of successful extensions: 13
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8
length of query: 239
length of database: 114,650
effective HSP length: 55
effective length of query: 184
effective length of database: 97,215
effective search space: 17887560
effective search space used: 17887560
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 42 (21.0 bits)