Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001146-TA|BGIBMGA001146-PA|IPR011701|Major facilitator
superfamily MFS_1
(384 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g25220.1 68417.m03629 transporter, putative similar to glycer... 33 0.24
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 32 0.74
At5g05470.1 68418.m00589 eukaryotic translation initiation facto... 31 0.98
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 31 0.98
At2g38060.1 68415.m04672 transporter-related low similarity to v... 31 1.3
At3g47420.1 68416.m05156 glycerol-3-phosphate transporter, putat... 30 3.0
At5g65580.1 68418.m08251 expressed protein 29 3.9
At4g10760.1 68417.m01756 methyltransferase MT-A70, putative simi... 29 3.9
At3g05670.1 68416.m00631 PHD finger family protein contains Pfam... 29 5.2
At5g46880.1 68418.m05777 homeobox-leucine zipper family protein ... 29 6.9
At5g37820.1 68418.m04554 major intrinsic family protein / MIP fa... 28 9.1
At3g14970.1 68416.m01893 zinc finger (C3HC4-type RING finger) fa... 28 9.1
At2g40290.2 68415.m04961 eukaryotic translation initiation facto... 28 9.1
At2g40290.1 68415.m04960 eukaryotic translation initiation facto... 28 9.1
At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note:... 28 9.1
>At4g25220.1 68417.m03629 transporter, putative similar to
glycerol-3-phosphate transporter (glycerol 3-phosphate
permease) [Homo sapiens] GI:7543982
Length = 504
Score = 33.5 bits (73), Expect = 0.24
Identities = 35/145 (24%), Positives = 59/145 (40%), Gaps = 4/145 (2%)
Query: 7 SGFLGGIALKKYSFRQVGLVGAGLF-ILGDILTIFVTRTYQLVFTFGVIRGAGFGVMIPV 65
+G LG L +F +G++G GLF L + ++ V+ G + P
Sbjct: 104 AGHLGD-RLDLRTFLTIGMIGTGLFTALFGVAFWANFHSFYYFLAVQVMAGLFQSIGWPC 162
Query: 66 SFTAFNSYFTKKRTTM-MSANQTMSSLASITFPMLVTYLLSEYGLRWTLALIMAIDLHLL 124
++F KKR M M +SL +I ++ + LL YG W+ + L
Sbjct: 163 IVAVLGNWFDKKRRGMIMGVWSAHTSLGNIAGSLIASGLL-RYGWGWSFLGPAFLMTFLG 221
Query: 125 FAMIVMHPVEWHLVKTNENNTELTS 149
+ + PV V+ + TE+ S
Sbjct: 222 IVVYLFLPVNPPTVEAERDGTEIDS 246
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 31.9 bits (69), Expect = 0.74
Identities = 17/62 (27%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
Query: 146 ELTSKCEVINVKKVEAFDEAVVKMLPGDD-KECEERETRVKKRESIQKHIIKTIYENIDL 204
++ SK + + KK+E + ++ L G+ E +E E ++K+ ES+ II +Y+
Sbjct: 563 KIASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEFEDKMKELESLCNPIIARMYQGAGP 622
Query: 205 DL 206
D+
Sbjct: 623 DM 624
>At5g05470.1 68418.m00589 eukaryotic translation initiation factor 2
subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
putative similar to SWISS-PROT:P20459 eukaryotic
translation initiation factor 2 alpha subunit (eIF-2-
alpha) [Saccharomyces cerevisiae]; identical to cDNA
cohesin GI:6682280
Length = 344
Score = 31.5 bits (68), Expect = 0.98
Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 3/61 (4%)
Query: 148 TSKCEVINVKKVEAFDEAVVKMLPGDDKE-CEERETRVKKRESIQKHIIKTIYENIDLDL 206
T V+ V + + + + + +DKE CEER + K SI +H+ +T+ +DL+
Sbjct: 72 TEPVMVLRVDRERGYIDLSKRRVSDEDKEACEERYNKSKLVHSIMRHVAETV--GVDLEE 129
Query: 207 L 207
L
Sbjct: 130 L 130
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 31.5 bits (68), Expect = 0.98
Identities = 17/45 (37%), Positives = 27/45 (60%), Gaps = 1/45 (2%)
Query: 157 KKVEAFDEAVVKMLPGDD-KECEERETRVKKRESIQKHIIKTIYE 200
KK+E EA ++ L + EC+E E ++K+ ESI II +Y+
Sbjct: 574 KKIEDSIEAAIEWLEANQLAECDEFEDKMKELESICNPIIAKMYQ 618
>At2g38060.1 68415.m04672 transporter-related low similarity to
vesicular glutamate transporter 3 [Homo sapiens]
GI:21213895, brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 512
Score = 31.1 bits (67), Expect = 1.3
Identities = 24/119 (20%), Positives = 50/119 (42%), Gaps = 2/119 (1%)
Query: 5 VYSGFLGGIALKKYSFRQVGLVGAGLFILGDILTIFVTRTYQL-VFTFGVIRGAGFGVMI 63
++S +GG + +Y ++V G L+ L +LT + L + G GV +
Sbjct: 149 IFSSVIGGALVDRYGGKRVLAWGVALWSLATLLTPWAAAHSTLALLCVRAFFGLAEGVAM 208
Query: 64 PVSFTAFNSYF-TKKRTTMMSANQTMSSLASITFPMLVTYLLSEYGLRWTLALIMAIDL 121
P T + +F +R + + + + ++ +L +LS G+ L ++ L
Sbjct: 209 PSMTTLLSRWFPMDERASAVGISMAGFHMGNVVGLLLTPLMLSSIGISGPFILFASLGL 267
>At3g47420.1 68416.m05156 glycerol-3-phosphate transporter, putative
/ glycerol 3-phosphate permease, putative similar to
cAMP inducible 2 protein [Mus musculus] GI:4580997,
glycerol-3-phosphate transporter (glycerol 3-phosphate
permease) [Homo sapiens] GI:7543982; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 523
Score = 29.9 bits (64), Expect = 3.0
Identities = 29/117 (24%), Positives = 54/117 (46%), Gaps = 14/117 (11%)
Query: 6 YSGFLGGIALKKYSFRQVGLVGAGLF-------ILGDILTIFVTRTYQLVFTFGVIRGAG 58
++G LG + F VG++G GLF G+I + + Q++ G+ + +G
Sbjct: 121 FAGHLGD-RMNLRIFLTVGMIGTGLFTSLFGVGYWGNIHSFYYFLIMQML--AGLFQSSG 177
Query: 59 FGVMIPVSFTAFNSYFTKKRTTMMSANQTMSSLASITFPMLVTYLLSEYGLRWTLAL 115
+ ++ V FN KKR +M +S+ +IT ++ +L YG W+ +
Sbjct: 178 WPSVVAVVGNWFNK---KKRGLIMGIWNAHTSVGNITGSLIAAAML-RYGWGWSFVV 230
>At5g65580.1 68418.m08251 expressed protein
Length = 102
Score = 29.5 bits (63), Expect = 3.9
Identities = 24/83 (28%), Positives = 40/83 (48%), Gaps = 3/83 (3%)
Query: 103 LLSEYGLRWTLALIMAIDLHLLFAMIVMHPVEWHLVKTNENNTELTSKCEVINVKKVEAF 162
LL +Y L+ T L I+ H F I P+ + + + +S ++V +V+A
Sbjct: 2 LLYDYLLQVTSILFDFIEFHEFF--IFGLPLLFAKSSPTKRRADSSSSTNFMSVMEVDA- 58
Query: 163 DEAVVKMLPGDDKECEERETRVK 185
D + KM+P D ERE R++
Sbjct: 59 DSSSTKMMPQTDGGDGERERRLR 81
>At4g10760.1 68417.m01756 methyltransferase MT-A70, putative similar
to (N6-adenosine)-methyltransferase [Mus musculus]
GI:10179948, m6A methyltransferase (MT-A70) [Homo
sapiens] GI:2460037; contains Pfam profile PF05063:
MT-A70 (S-adenosylmethionine-binding subunit of human
mRNA:m6A methyl-transferase (MTase))
Length = 685
Score = 29.5 bits (63), Expect = 3.9
Identities = 19/58 (32%), Positives = 29/58 (50%)
Query: 326 IMAAPDRFPAALALHILFSGVLMLIADPLIDIEDVYSSRYTEEKAKKQTLLKLPTLIH 383
IM P FP L L + + L + L D+E + S + +EK + +T +L LIH
Sbjct: 289 IMGRPPPFPLPLPLPVPSNQKLRSEEEDLKDVEALLSKKSFKEKQQSRTGEELLDLIH 346
>At3g05670.1 68416.m00631 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 883
Score = 29.1 bits (62), Expect = 5.2
Identities = 15/56 (26%), Positives = 25/56 (44%)
Query: 128 IVMHPVEWHLVKTNENNTELTSKCEVINVKKVEAFDEAVVKMLPGDDKECEERETR 183
+++ VEW VKT + C+ +V D V + DD++ E R +R
Sbjct: 80 VMLRNVEWPKVKTGPRGNRKITGCKSRKTNQVVVSDNEDVDLDDADDEDDEIRNSR 135
>At5g46880.1 68418.m05777 homeobox-leucine zipper family protein /
lipid-binding START domain-containing protein similar to
HD-Zip homeo domain OCL4 protein GI:8920425 from [Zea
mays]; contains Pfam PF00046: Homeobox domain and Pfam
PF01852: START domain
Length = 820
Score = 28.7 bits (61), Expect = 6.9
Identities = 11/28 (39%), Positives = 17/28 (60%)
Query: 344 SGVLMLIADPLIDIEDVYSSRYTEEKAK 371
SG L+L+ PL+ + Y RY E+ A+
Sbjct: 433 SGSLLLVLSPLVPTREAYFLRYVEQNAE 460
>At5g37820.1 68418.m04554 major intrinsic family protein / MIP
family protein contains Pfam profile: PF00230 major
intrinsic protein (MIP)
Length = 283
Score = 28.3 bits (60), Expect = 9.1
Identities = 15/53 (28%), Positives = 27/53 (50%)
Query: 47 LVFTFGVIRGAGFGVMIPVSFTAFNSYFTKKRTTMMSANQTMSSLASITFPML 99
++++ G I GA F + V+F F + + + A T S LAS+T ++
Sbjct: 89 MIYSTGHISGAHFNPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLM 141
>At3g14970.1 68416.m01893 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 220
Score = 28.3 bits (60), Expect = 9.1
Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Query: 150 KCEVI-NVKKVEAFDEAVVKMLPGDDKECEERETRV--KKRESIQKHIIKTIYEN 201
KC+ I NVK+V+ +V P DD + + V E++++H+ + EN
Sbjct: 114 KCDKISNVKEVDVVINVIVWRFPDDDDDDVDVRLAVAPASNEAVEQHLETVVVEN 168
>At2g40290.2 68415.m04961 eukaryotic translation initiation factor 2
subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
putative similar to Swiss-Prot:P05198 eukaryotic
translation initiation factor 2 subunit 1 (Eukaryotic
translation initiation factor 2 alpha subunit)
(eIF-2-alpha) (EIF- 2alpha) (EIF-2A) [Homo sapiens]
Length = 241
Score = 28.3 bits (60), Expect = 9.1
Identities = 16/54 (29%), Positives = 31/54 (57%), Gaps = 3/54 (5%)
Query: 153 VINVKKVEAFDEAVVKMLPGDD-KECEERETRVKKRESIQKHIIKTIYENIDLD 205
V+ V K + + + + + +D + CEER + K SI +H+ +T+ +IDL+
Sbjct: 77 VLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVAETL--SIDLE 128
>At2g40290.1 68415.m04960 eukaryotic translation initiation factor 2
subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
putative similar to Swiss-Prot:P05198 eukaryotic
translation initiation factor 2 subunit 1 (Eukaryotic
translation initiation factor 2 alpha subunit)
(eIF-2-alpha) (EIF- 2alpha) (EIF-2A) [Homo sapiens]
Length = 344
Score = 28.3 bits (60), Expect = 9.1
Identities = 16/54 (29%), Positives = 31/54 (57%), Gaps = 3/54 (5%)
Query: 153 VINVKKVEAFDEAVVKMLPGDD-KECEERETRVKKRESIQKHIIKTIYENIDLD 205
V+ V K + + + + + +D + CEER + K SI +H+ +T+ +IDL+
Sbjct: 77 VLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVAETL--SIDLE 128
>At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note:
CHX6a and CHX6b were originally 1 gene but were split
pased on alignments with other family members; may be a
pseudogene and requires futher investigation; monovalent
cation:proton antiporter family 2 (CPA2) member,
PMID:11500563
Length = 818
Score = 28.3 bits (60), Expect = 9.1
Identities = 21/83 (25%), Positives = 37/83 (44%), Gaps = 3/83 (3%)
Query: 223 FVSDVTYLAMEPTLLFSYKFTKIEVASCVMVGA-VSDLLARFGLAIF-TYFYAVDSRLLF 280
F+ ++ TLL + E + GA V+D+LA FG+ F +Y +
Sbjct: 195 FLQSISAFTSIDTLLKDLQIKHSEFGRIALSGAMVTDMLA-FGVTFFNAIYYEKLYGFMQ 253
Query: 281 FIGTCVTVFLRIALVTSSELWFV 303
+G C+ V + I +V + W +
Sbjct: 254 TVGFCLFVVVMICVVRPAMYWVI 276
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.328 0.141 0.408
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,050,703
Number of Sequences: 28952
Number of extensions: 314772
Number of successful extensions: 1202
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 1198
Number of HSP's gapped (non-prelim): 17
length of query: 384
length of database: 12,070,560
effective HSP length: 82
effective length of query: 302
effective length of database: 9,696,496
effective search space: 2928341792
effective search space used: 2928341792
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.8 bits)
S2: 60 (28.3 bits)
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