SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001145-TA|BGIBMGA001145-PA|IPR011701|Major facilitator
superfamily MFS_1
         (505 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizo...    33   0.12 
SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|c...    32   0.21 
SPCC5E4.03c |taf72||transcription factor TFIID complex subunit 5...    32   0.21 
SPAC11D3.18c |||nicotinic acid plasma membrane transporter |Schi...    31   0.28 
SPBC12C2.13c |fnx1|SPBC21D10.04c|MFS multidrug efflux transporte...    29   1.5  
SPAC6G9.15c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    28   2.6  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    28   2.6  
SPBC1683.08 |ght4||hexose transporter Ght4 |Schizosaccharomyces ...    27   4.6  
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa...    27   6.1  
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces...    27   6.1  
SPBC947.11c |elg1||DNA replication factor C complex subunit Elg1...    27   8.1  

>SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter
           Itr1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 575

 Score = 32.7 bits (71), Expect = 0.12
 Identities = 25/92 (27%), Positives = 40/92 (43%), Gaps = 2/92 (2%)

Query: 37  GASFIASTLDSVVNFSGFFVGPVIKTFSYRKVCFVGSTICALGLLFTAPAKSIGHILATY 96
           G  FI S         G   G +   F  + V  + S I  +G +    A  + H++   
Sbjct: 128 GKEFITSATSLGALLGGIIAGALADFFGRKPVIAIASIIIIVGSIVQVTAHHLWHMIVGR 187

Query: 97  SILGGFGVGLASSSSFVSLNHYF-SKKRGQAV 127
            ++ G+GVG+AS    + L+    SK RG+ V
Sbjct: 188 FVI-GWGVGIASLIIPLYLSEIAPSKIRGRLV 218


>SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 581

 Score = 31.9 bits (69), Expect = 0.21
 Identities = 23/115 (20%), Positives = 43/115 (37%)

Query: 42  ASTLDSVVNFSGFFVGPVIKTFSYRKVCFVGSTICALGLLFTAPAKSIGHILATYSILGG 101
           A  L   V +  +   P    +  R V  V      LG ++ A  K+  + +   + +G 
Sbjct: 100 AGVLFICVGYFTYLAMPATFLYGRRCVYLVCLLFGMLGSMWFALVKTTSNSIGNQAFIGI 159

Query: 102 FGVGLASSSSFVSLNHYFSKKRGQAVGLSMAGTGFGLMVMPQLVKLLLGEYGFRW 156
                 + + F     +F  +RG  +G+ +  T  G  + P     +    G+RW
Sbjct: 160 SEACAEALTQFSVSEVFFEHERGIKIGIYILSTSVGTYLGPLAAGYIASSQGWRW 214


>SPCC5E4.03c |taf72||transcription factor TFIID complex subunit 5
           Taf72|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 643

 Score = 31.9 bits (69), Expect = 0.21
 Identities = 44/175 (25%), Positives = 77/175 (44%), Gaps = 20/175 (11%)

Query: 171 GSCLLQPIKRHLKEVPVDAEMQNIKEMECIMESDEENEDKFEINPLVTHPMPKLTTQRSH 230
           G   LQP+K  L+++P+D EM+ I EM+   E      D    +P +     KL    + 
Sbjct: 215 GDAQLQPVK--LQQMPMDKEMEKIVEMDLEEEDMMHQNDPNNQSPKLLKEFRKLHEPNAE 272

Query: 231 SNMNHPSVRTIGMTRAKTCD-----KPLQDFEKNSKVYPGLTTAASVGVMPRVTSSGSMN 285
              + PS   I +   K  D     + ++D+ K   + P   +  SV +     ++ +MN
Sbjct: 273 ---DAPSRDYIPLPPHKGVDILSEVEAVKDWSKRLHLGP-RASLPSVCMYTFHHTNNNMN 328

Query: 286 EAARRRKVSVISNISNMDFTGSYLQLYLDTADEEAIQMKTIKKAEPVSEKKGFIR 340
            A    + S  S +    F  SY++L+   AD+     K++ K+  V +  G +R
Sbjct: 329 CA----EFSPDSTMIACGFQESYIRLWSIKADK-----KSLPKSTSVEDSDGSVR 374


>SPAC11D3.18c |||nicotinic acid plasma membrane transporter
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 498

 Score = 31.5 bits (68), Expect = 0.28
 Identities = 28/109 (25%), Positives = 51/109 (46%), Gaps = 6/109 (5%)

Query: 66  RKVCFVGSTICALGLLFTAPAKSIGHILATYSILGGFGVGLASSSSFVSLNHYFSKKRGQ 125
           R + F+ S   ++ +LF+    S G ++AT  ILG     L  + +     HY  K++ Q
Sbjct: 127 RMLAFI-SFAWSMTVLFSGFMSSYGGLIATRLILGLLEGCLFPALNLYLTTHYTRKEQCQ 185

Query: 126 AVGLSMAGTG----FGLMVMPQLVKLLLG-EYGFRWTVVILGALAFNAV 169
            +    A  G    FG +    L ++  G + G++W  ++ G ++F  V
Sbjct: 186 RLSYLFASAGLAGAFGGLFAYALEQVHAGNKEGWQWIYIVEGLVSFIGV 234


>SPBC12C2.13c |fnx1|SPBC21D10.04c|MFS multidrug efflux
           transporter|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 531

 Score = 29.1 bits (62), Expect = 1.5
 Identities = 22/99 (22%), Positives = 42/99 (42%)

Query: 57  GPVIKTFSYRKVCFVGSTICALGLLFTAPAKSIGHILATYSILGGFGVGLASSSSFVSLN 116
           G +   F  R      +    LG  +   ++S+  +    ++ G  G GL + SS +S +
Sbjct: 87  GRLSDIFGRRPTVVFANAAFTLGTFWCGISRSLPELCMARALAGIGGGGLGTMSSIISSD 146

Query: 117 HYFSKKRGQAVGLSMAGTGFGLMVMPQLVKLLLGEYGFR 155
               ++RG   G++    G G  +   L  L+   +G+R
Sbjct: 147 IVSLRERGTWQGITNIVWGIGGSLGGPLGGLIAQRWGWR 185


>SPAC6G9.15c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 498

 Score = 28.3 bits (60), Expect = 2.6
 Identities = 24/100 (24%), Positives = 45/100 (45%), Gaps = 2/100 (2%)

Query: 203 SDEENEDKFEINPLVTHPMPKLTTQRSHSNMNHPSVRTIGMTRAKTCDKPLQDFEKNSKV 262
           + +++E     N L  HP+   +T+ S +  N   V T      +   KPL +  + +  
Sbjct: 88  ASDDSEASLAENDL--HPLDNDSTRTSKTLKNSSEVLTASKLTDEGNSKPLLEEGEVAVS 145

Query: 263 YPGLTTAASVGVMPRVTSSGSMNEAARRRKVSVISNISNM 302
            P L  +  V +    +S   + +A + +K S  S+I+NM
Sbjct: 146 SPILLDSKDVIMGVTKSSKNLVEDAHKSKKASTNSSINNM 185


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 28.3 bits (60), Expect = 2.6
 Identities = 20/78 (25%), Positives = 34/78 (43%), Gaps = 2/78 (2%)

Query: 213 INPLVTHPMPKLTTQRSHSNMNHPSVRTIGMTRAKTCDKPLQD-FEKNSKVYP-GLTTAA 270
           I P   HP+P  +T  +    + P +  + M RA     P++  F   S   P  ++  +
Sbjct: 843 ITPADVHPLPPPSTSTTAGWNDAPMLGQLPMRRAAPSMAPVRSPFPGASSAQPAAMSRTS 902

Query: 271 SVGVMPRVTSSGSMNEAA 288
           SV  +P    + SM  +A
Sbjct: 903 SVSTLPPPPPTASMTASA 920


>SPBC1683.08 |ght4||hexose transporter Ght4 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 557

 Score = 27.5 bits (58), Expect = 4.6
 Identities = 9/33 (27%), Positives = 17/33 (51%)

Query: 367 WSGELQFRMLTPFFISNLGYNMDDTAFCLSMTA 399
           W G       TPF  +++G+ +     C++MT+
Sbjct: 412 WLGNFMVSFFTPFISNSIGFKLGYIYACINMTS 444


>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
           Brl2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 680

 Score = 27.1 bits (57), Expect = 6.1
 Identities = 16/48 (33%), Positives = 29/48 (60%), Gaps = 2/48 (4%)

Query: 310 QLYLDTADEEA-IQMKTIKKAEPVSEKKGFIR-RFIDLMDLDLLKDWS 355
           ++Y D  DE A ++   ++K + V+ ++   + R +DL DL+L KD S
Sbjct: 383 KVYKDKLDELANLETMVLEKKKAVATREAANKIRLVDLNDLELQKDLS 430


>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1727

 Score = 27.1 bits (57), Expect = 6.1
 Identities = 27/100 (27%), Positives = 43/100 (43%), Gaps = 5/100 (5%)

Query: 192 QNIKEMECIMESDE-ENEDKFEINPLVTHPMPKLTTQRSHSNMNHPSVRTIGMTRAKTCD 250
           + IK +E  + S + +NE       L+      L T RS    ++ S   I   + K+ D
Sbjct: 587 EQIKNLESELNSSKIKNESLLNERNLLKE---MLATSRSSILSHNSSAGNID-DKMKSID 642

Query: 251 KPLQDFEKNSKVYPGLTTAASVGVMPRVTSSGSMNEAARR 290
           +  ++ EKN +VY    TA    +  R     S  EA R+
Sbjct: 643 ESTRELEKNYEVYRNEMTAIQESLSKRNQDLLSEMEAIRK 682


>SPBC947.11c |elg1||DNA replication factor C complex subunit
           Elg1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 920

 Score = 26.6 bits (56), Expect = 8.1
 Identities = 15/40 (37%), Positives = 20/40 (50%)

Query: 205 EENEDKFEINPLVTHPMPKLTTQRSHSNMNHPSVRTIGMT 244
           EE +D  E NP V+    KLT+ +  + M    V  IG T
Sbjct: 400 EEEDDSDEFNPSVSRKKAKLTSSQFSNWMLVTGVTGIGKT 439


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.323    0.137    0.409 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,122,327
Number of Sequences: 5004
Number of extensions: 84500
Number of successful extensions: 259
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 253
Number of HSP's gapped (non-prelim): 13
length of query: 505
length of database: 2,362,478
effective HSP length: 76
effective length of query: 429
effective length of database: 1,982,174
effective search space: 850352646
effective search space used: 850352646
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 56 (26.6 bits)

- SilkBase 1999-2023 -