Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001140-TA|BGIBMGA001140-PA|IPR013838|Beta tubulin,
autoregulation binding site
(540 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15910.1 68416.m02012 hypothetical protein 32 1.1
At1g75260.1 68414.m08743 isoflavone reductase family protein sim... 32 1.1
At1g77180.1 68414.m08991 chromatin protein family contains Pfam ... 31 1.9
At5g04890.1 68418.m00513 small heat shock-like protein (RTM2) si... 31 2.6
At2g38810.3 68415.m04767 histone H2A, putative strong similarity... 31 2.6
At2g38810.2 68415.m04766 histone H2A, putative strong similarity... 31 2.6
At2g38810.1 68415.m04765 histone H2A, putative strong similarity... 31 2.6
At1g05320.1 68414.m00539 myosin-related similar to non-muscle my... 31 2.6
At2g34300.1 68415.m04196 dehydration-responsive protein-related ... 30 3.4
At1g63300.1 68414.m07156 expressed protein similar to Intracellu... 30 3.4
At4g05190.1 68417.m00781 kinesin-like protein A, putative kinesi... 30 4.5
At3g18810.1 68416.m02389 protein kinase family protein contains ... 30 4.5
At3g15880.2 68416.m02009 WD-40 repeat family protein contains Pf... 30 4.5
At3g15880.1 68416.m02008 WD-40 repeat family protein contains Pf... 30 4.5
At1g54560.1 68414.m06222 myosin, putative similar to myosin GI:4... 30 4.5
At5g13260.1 68418.m01523 expressed protein 29 5.9
At3g05270.1 68416.m00575 expressed protein similar to endosome-a... 29 5.9
At1g21630.1 68414.m02708 calcium-binding EF hand family protein ... 29 5.9
At5g51150.1 68418.m06342 expressed protein similar to unknown pr... 29 7.8
At2g45050.1 68415.m05608 zinc finger (GATA type) family protein ... 29 7.8
>At3g15910.1 68416.m02012 hypothetical protein
Length = 307
Score = 31.9 bits (69), Expect = 1.1
Identities = 10/21 (47%), Positives = 12/21 (57%)
Query: 286 RRWNSYEHFRWPEGGWWPPMC 306
R++ EH W E GWWP C
Sbjct: 54 RKFYCIEHPPWSESGWWPTQC 74
>At1g75260.1 68414.m08743 isoflavone reductase family protein
similar to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: Isoflavone reductase
Length = 593
Score = 31.9 bits (69), Expect = 1.1
Identities = 19/43 (44%), Positives = 29/43 (67%), Gaps = 2/43 (4%)
Query: 373 EKMVDLER-ALHEAKQRL-DDAEQKLQKEMLQRSSLETQKLEL 413
+K+ ++E+ AL E K+ L DD QKL+ + Q+ S ET+KL L
Sbjct: 193 QKIEEIEKIALQERKKVLHDDGVQKLEADQGQQKSKETEKLAL 235
>At1g77180.1 68414.m08991 chromatin protein family contains Pfam
domain, PF02731: SKIP/SNW domain found in chromatin
proteins.
Length = 613
Score = 31.1 bits (67), Expect = 1.9
Identities = 39/159 (24%), Positives = 60/159 (37%), Gaps = 18/159 (11%)
Query: 258 KPPQHPTGVRASPRPRRIRNEETPPDLHRRWNSYEHFRWPEGGWWPPMCWCHGPPIPPPC 317
KP Q + + + R IR E P D ++H R P PP+ H PP P
Sbjct: 192 KPSQQSSAFNSGAKERIIRMVEMPVDPLDP-PKFKHKRVPRASGSPPVPVMHSPP-RPVT 249
Query: 318 CMHDRSWPSHPSLPQTTVRGGFKNDAEDRVRRLEADKXXXXXXXXXXXXXISAQTEKMVD 377
+ W P + G+ + +RL AD +
Sbjct: 250 VKDQQDWKIPPCISNWKNPKGYTIPLD---KRLAADGRGLQDVQI---------NDNFAK 297
Query: 378 LERALHEAKQRLDDA---EQKLQKEMLQRSSLETQKLEL 413
L AL+ A+Q+ +A K+QKEM+ + E ++ EL
Sbjct: 298 LSEALYVAEQKAREAVSMRSKVQKEMVMKDK-ERKEQEL 335
>At5g04890.1 68418.m00513 small heat shock-like protein (RTM2)
similar to 17.9 kDa heat-shock protein [Helianthus
annuus] GI:11990130; contains Pfam profile PF00011:
Hsp20/alpha crystallin family; supporting cDNA
gi|7407072|gb|AF208051.1|AF208051; identical to cDNA
small heat shock-like protein (RTM2) GI:7407072, small
heat shock-like protein [Arabidopsis thaliana]
GI:7407073
Length = 366
Score = 30.7 bits (66), Expect = 2.6
Identities = 14/43 (32%), Positives = 27/43 (62%)
Query: 370 AQTEKMVDLERALHEAKQRLDDAEQKLQKEMLQRSSLETQKLE 412
A+ ++ ++ + EAK + + A +KLQ+E+ + LE +KLE
Sbjct: 170 AKAKEEAEMRKLQEEAKAKEEAAAKKLQEEIEAKEKLEERKLE 212
>At2g38810.3 68415.m04767 histone H2A, putative strong similarity
to histone H2A.F/Z Arabidopsis thaliana GI:2407800;
contains Pfam profile PF00125 Core histone
H2A/H2B/H3/H4
Length = 136
Score = 30.7 bits (66), Expect = 2.6
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Query: 5 GRGGS--LVAATIAALHNRTAGEKPPIPIKSRANTESKHSQLEKEISEKQSRRASGGSER 62
G+GG L A T AA N+ + +K I SRA + ++ +++ ++ S G+
Sbjct: 3 GKGGKGLLAAKTTAAAANKDSVKKKSISRSSRAGIQFPVGRIHRQLKQRVSAHGRVGATA 62
Query: 63 PLSTAS 68
+ TAS
Sbjct: 63 AVYTAS 68
>At2g38810.2 68415.m04766 histone H2A, putative strong similarity
to histone H2A.F/Z Arabidopsis thaliana GI:2407800;
contains Pfam profile PF00125 Core histone
H2A/H2B/H3/H4
Length = 136
Score = 30.7 bits (66), Expect = 2.6
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Query: 5 GRGGS--LVAATIAALHNRTAGEKPPIPIKSRANTESKHSQLEKEISEKQSRRASGGSER 62
G+GG L A T AA N+ + +K I SRA + ++ +++ ++ S G+
Sbjct: 3 GKGGKGLLAAKTTAAAANKDSVKKKSISRSSRAGIQFPVGRIHRQLKQRVSAHGRVGATA 62
Query: 63 PLSTAS 68
+ TAS
Sbjct: 63 AVYTAS 68
>At2g38810.1 68415.m04765 histone H2A, putative strong similarity
to histone H2A.F/Z Arabidopsis thaliana GI:2407800;
contains Pfam profile PF00125 Core histone
H2A/H2B/H3/H4
Length = 136
Score = 30.7 bits (66), Expect = 2.6
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Query: 5 GRGGS--LVAATIAALHNRTAGEKPPIPIKSRANTESKHSQLEKEISEKQSRRASGGSER 62
G+GG L A T AA N+ + +K I SRA + ++ +++ ++ S G+
Sbjct: 3 GKGGKGLLAAKTTAAAANKDSVKKKSISRSSRAGIQFPVGRIHRQLKQRVSAHGRVGATA 62
Query: 63 PLSTAS 68
+ TAS
Sbjct: 63 AVYTAS 68
>At1g05320.1 68414.m00539 myosin-related similar to non-muscle
myosin II heavy chain (GI:19879404) [Loligo pealei];
ESTs gb|AA042402,gb|ATTS1380 come from this gene
Length = 828
Score = 30.7 bits (66), Expect = 2.6
Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 6/80 (7%)
Query: 343 AEDRVRRLEADKXXXXXXXXXXXXXISAQTEKMVDLERALHEAKQRLDDAEQKLQKEMLQ 402
AE++++ LEA + +S EK DL L+ ++L +E++L+K+ +
Sbjct: 256 AEEKLKDLEAIQVKNSSLEAT----LSVAMEKERDLSENLNAVMEKLKSSEERLEKQARE 311
Query: 403 RSSLETQKLEL--LSKLSEV 420
T+ +EL L K SE+
Sbjct: 312 IDEATTRSIELEALHKHSEL 331
>At2g34300.1 68415.m04196 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 770
Score = 30.3 bits (65), Expect = 3.4
Identities = 14/41 (34%), Positives = 22/41 (53%)
Query: 32 KSRANTESKHSQLEKEISEKQSRRASGGSERPLSTASETSE 72
+S T+ + +QLE+ E +S +G E+ ASET E
Sbjct: 144 ESDETTQKEKTQLEESTEENKSEDGNGNEEKAEENASETEE 184
>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]; similar to Myosin II heavy
chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
discoideum]
Length = 1029
Score = 30.3 bits (65), Expect = 3.4
Identities = 17/49 (34%), Positives = 30/49 (61%), Gaps = 1/49 (2%)
Query: 371 QTEKMVDLERALHEAKQRLDDAEQKLQKEMLQRSSLETQKLELLSKLSE 419
Q E+ +L L + K+ + +AE LQ+E +++ LE+ K+ L+ K SE
Sbjct: 750 QAEQAENLRVDLEKTKKSVMEAEASLQRENMKKIELES-KISLMRKESE 797
>At4g05190.1 68417.m00781 kinesin-like protein A, putative kinesin
like protein A, Arabidopsis thaliana, gb:Q07970
Length = 790
Score = 29.9 bits (64), Expect = 4.5
Identities = 17/70 (24%), Positives = 32/70 (45%)
Query: 344 EDRVRRLEADKXXXXXXXXXXXXXISAQTEKMVDLERALHEAKQRLDDAEQKLQKEMLQR 403
++R++ LE + +S + + ++ +HE + RL D E++L + L R
Sbjct: 356 KERIKMLEQELAFAKEKLKMVDLSMSHTMTEFEEQKQCMHELQDRLADTERQLFEGELLR 415
Query: 404 SSLETQKLEL 413
L LEL
Sbjct: 416 KKLHNTILEL 425
>At3g18810.1 68416.m02389 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 700
Score = 29.9 bits (64), Expect = 4.5
Identities = 14/39 (35%), Positives = 17/39 (43%), Gaps = 5/39 (12%)
Query: 307 WCHGPPIPPPCCMHDRSWPSHPSLPQTTVRGGFKNDAED 345
W + PP PPP SW P P V GG ++ D
Sbjct: 263 WMNSPPPPPP-----GSWQPSPPPPPPPVSGGMNGNSSD 296
>At3g15880.2 68416.m02009 WD-40 repeat family protein contains Pfam
profile: PF00400 WD domain, G-beta repeat (7 copies)
Length = 1135
Score = 29.9 bits (64), Expect = 4.5
Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 3/44 (6%)
Query: 297 PEGGWWPPMCWCHGP--PIPPPCCMHDRSWPSHPSLPQTTVRGG 338
P+ G +PP+ HGP P P P W +PS+ TV G
Sbjct: 225 PKVGGFPPL-GAHGPFQPTPAPLTTSLAGWMPNPSVQHPTVSAG 267
>At3g15880.1 68416.m02008 WD-40 repeat family protein contains Pfam
profile: PF00400 WD domain, G-beta repeat (7 copies)
Length = 1137
Score = 29.9 bits (64), Expect = 4.5
Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 3/44 (6%)
Query: 297 PEGGWWPPMCWCHGP--PIPPPCCMHDRSWPSHPSLPQTTVRGG 338
P+ G +PP+ HGP P P P W +PS+ TV G
Sbjct: 225 PKVGGFPPL-GAHGPFQPTPAPLTTSLAGWMPNPSVQHPTVSAG 267
>At1g54560.1 68414.m06222 myosin, putative similar to myosin GI:433663
from [Arabidopsis thaliana]
Length = 1529
Score = 29.9 bits (64), Expect = 4.5
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Query: 368 ISAQTEKMVDLERALHEAKQRLDDAEQKLQKEMLQRSSLETQKLELLSKLSE 419
I A TE++ L+ L + KQR DDA +K E + S +KLE K ++
Sbjct: 978 IEALTEEVEGLKANLEQEKQRADDATRKFD-EAQESSEDRKKKLEDTEKKAQ 1028
>At5g13260.1 68418.m01523 expressed protein
Length = 537
Score = 29.5 bits (63), Expect = 5.9
Identities = 21/79 (26%), Positives = 35/79 (44%), Gaps = 3/79 (3%)
Query: 342 DAEDRVRRLEADKXXXXXXXXXXXXXISAQTEKMVDLERALHEAKQRLDDAEQKLQKEML 401
+AE RVR LE +S + + E AL +A+Q D ++
Sbjct: 220 EAEARVRELEKQVTSLGEGVSLEAKLLSRKEAALRQREAALKDARQNRDGTN---RETTA 276
Query: 402 QRSSLETQKLELLSKLSEV 420
RS +ET KLE + ++++
Sbjct: 277 LRSQVETAKLETAAIVAQL 295
>At3g05270.1 68416.m00575 expressed protein similar to
endosome-associated protein (EEA1) (GI:1016368) [Homo
sapiens]; similar to smooth muscle myosin heavy chain
(GI:4417214) [Homo sapiens; contains Pfam profile
PF05911: Plant protein of unknown function (DUF869)
Length = 615
Score = 29.5 bits (63), Expect = 5.9
Identities = 13/38 (34%), Positives = 25/38 (65%)
Query: 377 DLERALHEAKQRLDDAEQKLQKEMLQRSSLETQKLELL 414
+LE L E+ ++++D +++L K + S LET++ E L
Sbjct: 409 ELELLLGESGKQMEDLQRQLNKAQVNLSELETRRAEKL 446
>At1g21630.1 68414.m02708 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain; ESTs gb|T44428 and gb|AA395440 come from this
gene
Length = 1218
Score = 29.5 bits (63), Expect = 5.9
Identities = 33/161 (20%), Positives = 65/161 (40%), Gaps = 11/161 (6%)
Query: 259 PPQHPTGVRASPRPRRIRNEETPPDLHRRWNSYEHFRWPE--GGWWPPMCWCHGPPIPPP 316
PP P+ + S + P + W + F+ GG PP PP P P
Sbjct: 499 PPVFPSSIIHSESMFTSPGQSVAPHGNASWGHPQGFQQQPHPGGLRPPAGPKGKPPRPVP 558
Query: 317 CCMHDRSWPSHPSLPQTTVRGGFKNDAEDRVRRLEADKXXXXXXXXXXXXXISAQTEKMV 376
D P+ P+ + + D++ + E D +A +K+
Sbjct: 559 LSPSDGM--VQPTQPKRKMPV-LEKPLVDQLSKEEQDSLNTKFEEA------TAVDKKVD 609
Query: 377 DLERALHEAKQRLDDAEQKLQKEMLQRSSLETQKLELLSKL 417
+LE+ + ++KQ++D K+Q+ +L +S + + E+ ++
Sbjct: 610 ELEKEIADSKQKIDFFRAKMQELVLYKSRCDNRYNEIAERV 650
>At5g51150.1 68418.m06342 expressed protein similar to unknown
protein (gb|AAD46013.1)
Length = 531
Score = 29.1 bits (62), Expect = 7.8
Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
Query: 368 ISAQTEKMVDLERALHEAKQRLDDAEQKLQKEMLQRSSLETQKLEL 413
IS ++ + ER L EA++RL DA +LQ+ QRS+ EL
Sbjct: 19 ISPLSQDSSEAERRLREAEERLRDAMAELQRR--QRSAARGSHAEL 62
>At2g45050.1 68415.m05608 zinc finger (GATA type) family protein
identical to cDNA GATA transcription factor 2 GI:2959731
Length = 264
Score = 29.1 bits (62), Expect = 7.8
Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Query: 23 AGEKPPIPIKSRANTESKHSQLEKEISEKQSRRASGGSERPLSTASETSE 72
AG P+P++S + HS + + ++QS GG R S++SET+E
Sbjct: 127 AGTWSPMPLESEH--QQLHSAAKFKPKKEQSGGGGGGGGRHQSSSSETTE 174
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.312 0.128 0.390
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,532,148
Number of Sequences: 28952
Number of extensions: 462475
Number of successful extensions: 1270
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1253
Number of HSP's gapped (non-prelim): 30
length of query: 540
length of database: 12,070,560
effective HSP length: 85
effective length of query: 455
effective length of database: 9,609,640
effective search space: 4372386200
effective search space used: 4372386200
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 62 (29.1 bits)
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