Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001138-TA|BGIBMGA001138-
PA|IPR005135|Endonuclease/exonuclease/phosphatase
         (404 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32F12.01c ||SPBC685.10c|inositol phosphosphingolipid phospho...   139   6e-34
SPBC557.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    27   4.7  
SPAC644.07 |||Rieske ISP assembly protein|Schizosaccharomyces po...    27   6.2  
SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb...    26   8.3  

>SPBC32F12.01c ||SPBC685.10c|inositol phosphosphingolipid
           phospholipase C |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 424

 Score =  139 bits (337), Expect = 6e-34
 Identities = 92/285 (32%), Positives = 149/285 (52%), Gaps = 19/285 (6%)

Query: 11  GIPVVSKNKKERYEAISTYLLKSEHNIVCLQEVWSEKDYLYLKENLKNVLPYSYYFYSGV 70
           G+  VSK + ER +A+   L K +++IV LQEVWS  D+  ++  +   L YS +F+S  
Sbjct: 18  GLRFVSKYRTERLKAVGEKLAKCDYDIVLLQEVWSIYDFQEIRNLVSCNLVYSRFFHSAA 77

Query: 71  LGSGLCVFSKWVIQDVFFHQWPLNGYIHKIHHGDWFGGKGVGLCRIKF-GERLINVYCTH 129
           +G+GL +FSK+ I +   +++PLNG       GDW+ GKGV    ++    R+I+++ TH
Sbjct: 78  MGAGLAMFSKFPIIESSMNKYPLNGRPQAFWRGDWYVGKGVATASLQHPSGRIISLFNTH 137

Query: 130 LHAEYHED-DMYLAHRVLQAYSTAEFVKLTSSPADVSILAGDLNTAPGDLSYKIISQLPS 188
           LHA Y +  D YL HR+ QA+  ++ ++       + I AGD N  P  + ++II+    
Sbjct: 138 LHAPYGKGADTYLCHRLSQAWYISKLLRAAVQRGHIVIAAGDFNIQPLSVPHEIITSYGL 197

Query: 189 LLD------------PYNMKFEGTNPLIAKASGTSDNLNNSYSDPKQVKAYPE--GKRID 234
           + D            P N        L+  A  T D+  N++ +    K   +   KR+D
Sbjct: 198 VNDAWLSVYPDQVEHPPNRFSMNDKELVEIAGTTCDSRLNTWRENISSKDMDDFVAKRLD 257

Query: 235 HILFHTNHSWEARVVNFGNPLPDRVPEQQFSYSDHNAVSLELHLK 279
           ++ FH+  + EA+  N      +RVP+   SYSDH A+   L +K
Sbjct: 258 YV-FHSPSTCEAK--NAKVVFLERVPKLDCSYSDHFAIETVLSIK 299


>SPBC557.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 203

 Score = 27.1 bits (57), Expect = 4.7
 Identities = 13/30 (43%), Positives = 18/30 (60%), Gaps = 1/30 (3%)

Query: 204 IAKASGTSD-NLNNSYSDPKQVKAYPEGKR 232
           ++KA   SD  LNNS  D +++K YP   R
Sbjct: 33  LSKARKPSDFELNNSIEDAEKIKTYPAALR 62


>SPAC644.07 |||Rieske ISP assembly protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 449

 Score = 26.6 bits (56), Expect = 6.2
 Identities = 13/33 (39%), Positives = 16/33 (48%)

Query: 237 LFHTNHSWEARVVNFGNPLPDRVPEQQFSYSDH 269
           LF  N S  A  V+    LPD  P   FS++ H
Sbjct: 411 LFVMNKSSPADAVDMAKELPDNPPSTPFSFNVH 443


>SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 420

 Score = 26.2 bits (55), Expect = 8.3
 Identities = 21/85 (24%), Positives = 34/85 (40%), Gaps = 3/85 (3%)

Query: 186 LPSLLDPYNMKFEGTNPLIAKASGT-SDNLNNSYSDPKQVKAYPEGKRIDHILFHTNHSW 244
           LP   D      E +N  + K+S + S N +N++   K +  + EG    H   H +HS 
Sbjct: 255 LPKSSDSSETSHESSNSNLKKSSESGSTNHHNNHESDKDL--HHEGHHHHHHHHHHHHSH 312

Query: 245 EARVVNFGNPLPDRVPEQQFSYSDH 269
           +    +      + VP       DH
Sbjct: 313 DDDPSSPAEKKQNHVPSPSEKIQDH 337


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.320    0.137    0.421 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,981,162
Number of Sequences: 5004
Number of extensions: 89936
Number of successful extensions: 243
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 237
Number of HSP's gapped (non-prelim): 5
length of query: 404
length of database: 2,362,478
effective HSP length: 74
effective length of query: 330
effective length of database: 1,992,182
effective search space: 657420060
effective search space used: 657420060
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 55 (26.2 bits)