Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001137-TA|BGIBMGA001137-PA|IPR000398|Thymidylate
synthase
(253 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g16370.1 68415.m01873 bifunctional dihydrofolate reductase-th... 352 2e-97
At4g34570.1 68417.m04912 bifunctional dihydrofolate reductase-th... 350 6e-97
At2g21550.1 68415.m02565 bifunctional dihydrofolate reductase-th... 192 1e-49
At1g13410.1 68414.m01564 pentatricopeptide (PPR) repeat-containi... 30 1.7
At3g29035.1 68416.m03632 no apical meristem (NAM) family protein... 28 7.0
At5g38900.1 68418.m04705 DSBA oxidoreductase family protein cont... 27 9.3
At5g20080.1 68418.m02391 NADH-cytochrome b5 reductase, putative ... 27 9.3
At3g13150.1 68416.m01645 pentatricopeptide (PPR) repeat-containi... 27 9.3
>At2g16370.1 68415.m01873 bifunctional dihydrofolate
reductase-thymidylate synthase 1 / DHFR-TS (THY-1)
identical to GP:289193:L08593 [SP|Q05762]
Length = 519
Score = 352 bits (865), Expect = 2e-97
Identities = 160/244 (65%), Positives = 184/244 (75%)
Query: 10 TLPLLTPKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFLDNLGFTDREEG 69
+ PLLT KRVF RGV+EELLW ISGST++K L KG+HIWD N SR +LD +G T+REEG
Sbjct: 276 SFPLLTTKRVFWRGVVEELLWFISGSTNAKVLQEKGIHIWDGNASREYLDGIGLTEREEG 335
Query: 70 DLGPVYGFQWRHSGAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAWNSSDLKK 129
DLGPVYGFQWRH GAKY D DYTGQG DQL VID IKNNP DRR+++ AWN SDLK
Sbjct: 336 DLGPVYGFQWRHFGAKYTDMHADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKL 395
Query: 130 MALPPCHCLAQFYVADGKLSCLLYQRSADMGLGVPFNIASYSLLTHMIAHVTGYQAGEFI 189
MALPPCH AQFYVA+G+LSC +YQRSADMGLGVPFNIASYSLLT M+AHV G+FI
Sbjct: 396 MALPPCHMFAQFYVAEGELSCQMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFI 455
Query: 190 HTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIESIDDFKYEDFIIKDYKPHPKIEM 249
H GD HVY H+ PL+ QL PK FP ++ + + ID F DF + Y PH KIEM
Sbjct: 456 HVLGDAHVYKTHVRPLQEQLLNLPKPFPVMKINPEKKQIDSFVASDFDLTGYDPHKKIEM 515
Query: 250 EMAV 253
+MAV
Sbjct: 516 KMAV 519
>At4g34570.1 68417.m04912 bifunctional dihydrofolate
reductase-thymidylate synthase 2 / DHFR-TS (THY-2)
identical to SP|Q05763
Length = 565
Score = 350 bits (860), Expect = 6e-97
Identities = 160/251 (63%), Positives = 190/251 (75%), Gaps = 1/251 (0%)
Query: 3 RYSLHNNTLPLLTPKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFLDNLG 62
+++L N PLLT KRVF RGV+EELLW ISGST++K L KG+ IWD N SR +LD +G
Sbjct: 316 KFNLRRN-FPLLTTKRVFWRGVVEELLWFISGSTNAKVLQEKGIRIWDGNASRAYLDGIG 374
Query: 63 FTDREEGDLGPVYGFQWRHSGAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICAW 122
T+REEGDLGPVYGFQWRH GAKY D DYTGQG DQL VI+ IKNNP DRR+++ AW
Sbjct: 375 LTEREEGDLGPVYGFQWRHFGAKYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAW 434
Query: 123 NSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRSADMGLGVPFNIASYSLLTHMIAHVTG 182
N SDLK MALPPCH AQFYVA+G+LSC +YQRSADMGLGVPFNIASYSLLT ++AHV
Sbjct: 435 NPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCD 494
Query: 183 YQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIESIDDFKYEDFIIKDYK 242
G+FIH GD HVY NH+ PL+ QL+ PK FP L+ + + ID F +DF + Y
Sbjct: 495 LVPGDFIHVIGDAHVYKNHVRPLQEQLENPPKPFPVLKINPEKKDIDSFVADDFELIGYD 554
Query: 243 PHPKIEMEMAV 253
PH KI+M+MAV
Sbjct: 555 PHKKIDMKMAV 565
>At2g21550.1 68415.m02565 bifunctional dihydrofolate
reductase-thymidylate synthase, putative / DHFR-TS,
putative similar to THY-1 [SP| Q05762] and THY-2
[SP|Q05763] from Arabidopsis thaliana; contains Pfam
profiles PF00303 thymidylate synthase and PF00186
dihydrofolate reductase
Length = 492
Score = 192 bits (469), Expect = 1e-49
Identities = 97/234 (41%), Positives = 147/234 (62%), Gaps = 13/234 (5%)
Query: 3 RYSLHNNTLPLLTPKRVFTRGVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFLDNLG 62
R++L T PLLT K++F GV+EE+L +ISGS + K G HIWD + ++ +LD+ G
Sbjct: 265 RFNLRK-TFPLLTTKKIFWLGVVEEILQLISGSNNPKE---NGSHIWDTDEAKEYLDSFG 320
Query: 63 FTDREE-GDLGPVYGFQWRHSGAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRMLICA 121
EE GD ++G W+H A+++ Q QL VI+ IKNNP D+R+++ A
Sbjct: 321 VNATEEDGDNPFLHGLHWKHCDARFVI-------QEFSQLSDVINKIKNNPHDQRIMLAA 373
Query: 122 WNSSDLKKMALPPCHCLAQFYVADGKLSCLLYQRSADMGLGVPFNIASYSLLTHMIAHVT 181
N D K +++ PCH QFYVA+G++SC +YQ S + +G+PF+IA+YSLLT +IAHV
Sbjct: 374 CNPLDFK-LSVSPCHTFTQFYVANGEVSCQIYQSSTEASIGIPFSIATYSLLTCIIAHVC 432
Query: 182 GYQAGEFIHTTGDTHVYLNHIEPLKIQLQREPKQFPTLEFARKIESIDDFKYED 235
AG+FIH G ++ H++ ++ QLQ PK FP L+ + + +D+F+ D
Sbjct: 433 DLGAGDFIHVIGQAYINKAHVKAIQKQLQISPKPFPILKINPEKKKMDNFEASD 486
>At1g13410.1 68414.m01564 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 511
Score = 29.9 bits (64), Expect = 1.7
Identities = 19/63 (30%), Positives = 35/63 (55%), Gaps = 3/63 (4%)
Query: 138 LAQFYVADGKLSCLL--YQRSADMGLGVPFNIASYSLLTHMIAHVTGYQAGEFIHTTGDT 195
L + Y +G++S +L ++R D G VP N A+ +L+ A + + G+++H G+T
Sbjct: 195 LIKGYAQNGRVSEVLGSFKRMVDEGSVVP-NDATMTLVLSACAKLGAFDFGKWVHKYGET 253
Query: 196 HVY 198
Y
Sbjct: 254 LGY 256
>At3g29035.1 68416.m03632 no apical meristem (NAM) family protein
contains Pfam PF02365 : No apical meristem (NAM)
protein; similar to cup-shaped cotyledon CUC2
(GI:1944132) [Arabidopsis thaliana]
Length = 318
Score = 27.9 bits (59), Expect = 7.0
Identities = 13/40 (32%), Positives = 21/40 (52%)
Query: 23 GVIEELLWMISGSTDSKALAAKGVHIWDANGSRNFLDNLG 62
G++ + + +TD K+L A +GS NFL N+G
Sbjct: 212 GLLSHVTYFSDQTTDDKSLVADFKTTMFGSGSTNFLPNIG 251
>At5g38900.1 68418.m04705 DSBA oxidoreductase family protein
contains Pfam profile: PF01323 DSBA-like thioredoxin
domain
Length = 217
Score = 27.5 bits (58), Expect = 9.3
Identities = 16/35 (45%), Positives = 20/35 (57%), Gaps = 3/35 (8%)
Query: 160 GLGVPFNIASY---SLLTHMIAHVTGYQAGEFIHT 191
GLG+ F+ A SL +H + H TG QA E HT
Sbjct: 92 GLGLEFDTAGLTGNSLDSHRLIHYTGKQAPEKQHT 126
>At5g20080.1 68418.m02391 NADH-cytochrome b5 reductase, putative
similar to SP|P36060 NADH-cytochrome b5 reductase
precursor (EC 1.6.2.2) {Saccharomyces cerevisiae};
contains Pfam profiles PF00175: Oxidoreductase
NAD-binding domain, PF00970: oxidoreductase, FAD-binding
Length = 328
Score = 27.5 bits (58), Expect = 9.3
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Query: 72 GPVYGFQWRHSGAKYIDCQTDYTGQGIDQLQSVIDSIKNNPADRRML 118
GPV F++ + K+I G GI + VID+I NP D +
Sbjct: 176 GPVEKFKYSPNMKKHIGMIAG--GSGITPMLQVIDAIVKNPEDNTQI 220
>At3g13150.1 68416.m01645 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 551
Score = 27.5 bits (58), Expect = 9.3
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Query: 207 IQLQREPKQFPTL-EFARKIESIDDFKYEDFIIK 239
I+ RE K+F T+ E + + DD K EDF+I+
Sbjct: 94 IRRLREAKKFSTIDEVLQYQKKFDDIKSEDFVIR 127
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.321 0.138 0.428
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,520,825
Number of Sequences: 28952
Number of extensions: 278303
Number of successful extensions: 607
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 599
Number of HSP's gapped (non-prelim): 8
length of query: 253
length of database: 12,070,560
effective HSP length: 79
effective length of query: 174
effective length of database: 9,783,352
effective search space: 1702303248
effective search space used: 1702303248
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 58 (27.5 bits)
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