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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001132-TA|BGIBMGA001132-PA|IPR000910|HMG1/2 (high
mobility group) box, IPR000135|High mobility group box HMG1 and HMG2,
IPR009071|High mobility group box
         (297 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_21901| Best HMM Match : No HMM Matches (HMM E-Value=.)             146   2e-35
SB_21059| Best HMM Match : No HMM Matches (HMM E-Value=.)              66   2e-11
SB_23256| Best HMM Match : HMG_box (HMM E-Value=3.3e-22)               66   4e-11
SB_7134| Best HMM Match : HMG_box (HMM E-Value=2e-16)                  64   1e-10
SB_1832| Best HMM Match : HMG_box (HMM E-Value=1.7e-22)                63   3e-10
SB_37758| Best HMM Match : No HMM Matches (HMM E-Value=.)              61   9e-10
SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)                     60   3e-09
SB_16438| Best HMM Match : HMG_box (HMM E-Value=7.2e-31)               51   1e-06
SB_18968| Best HMM Match : HMG_box (HMM E-Value=1.2e-19)               50   3e-06
SB_42643| Best HMM Match : No HMM Matches (HMM E-Value=.)              48   1e-05
SB_12182| Best HMM Match : No HMM Matches (HMM E-Value=.)              45   9e-05
SB_46509| Best HMM Match : HMG_box (HMM E-Value=4.2e-10)               44   1e-04
SB_33348| Best HMM Match : HMG_box (HMM E-Value=3.2e-34)               44   2e-04
SB_31139| Best HMM Match : No HMM Matches (HMM E-Value=.)              44   2e-04
SB_23680| Best HMM Match : No HMM Matches (HMM E-Value=.)              43   3e-04
SB_29734| Best HMM Match : No HMM Matches (HMM E-Value=.)              42   6e-04
SB_16422| Best HMM Match : HMG_box (HMM E-Value=8.7e-26)               41   0.001
SB_13764| Best HMM Match : No HMM Matches (HMM E-Value=.)              41   0.001
SB_3516| Best HMM Match : No HMM Matches (HMM E-Value=.)               41   0.001
SB_29576| Best HMM Match : No HMM Matches (HMM E-Value=.)              39   0.004
SB_53304| Best HMM Match : HMG_box (HMM E-Value=1.9e-32)               38   0.007
SB_26162| Best HMM Match : HMG_box (HMM E-Value=1.5e-31)               37   0.017
SB_25642| Best HMM Match : HMG_box (HMM E-Value=1.3e-29)               37   0.023
SB_10678| Best HMM Match : HMG_box (HMM E-Value=1.3e-29)               37   0.023
SB_2908| Best HMM Match : HMG_box (HMM E-Value=1.5e-08)                35   0.092
SB_27742| Best HMM Match : HMG_box (HMM E-Value=1.3e-24)               33   0.28 
SB_52386| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   0.37 
SB_49511| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   0.37 
SB_41600| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   0.65 
SB_3226| Best HMM Match : RNA_pol_Rpb2_1 (HMM E-Value=0.021)           30   2.0  
SB_6714| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   2.6  
SB_32415| Best HMM Match : Gag_MA (HMM E-Value=3.2)                    29   3.5  
SB_29054| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   6.0  
SB_26233| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   6.0  
SB_23345| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   6.0  

>SB_21901| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 192

 Score =  146 bits (354), Expect = 2e-35
 Identities = 75/167 (44%), Positives = 105/167 (62%), Gaps = 3/167 (1%)

Query: 105 NKPRGRMTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQ 164
           NKP+G  +AY FF+Q  RE+ +++    S+  A FSK  AE+W  MSE+EK+ F + A +
Sbjct: 10  NKPKGAKSAYNFFLQDQREKLQREEGKFSL--ADFSKVSAEKWKNMSEEEKETFVQKAGK 67

Query: 165 DKHRFDLEMQNYVPPKDMKVRGRKRQ-QMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEY 223
           DK RF  EMQ+Y PP   +   +KR+ Q KDPN PKR LSA+F F N +R  VK  NP  
Sbjct: 68  DKERFKEEMQSYTPPPSEESGKKKRKKQTKDPNKPKRCLSAYFHFINLKRDDVKKDNPNA 127

Query: 224 TMGDIAKELGRRWAAADPETKAKYDALSEKDKARYDREMTAYKKGPL 270
           + G ++K LG  W+    + K +Y  +++KDK RY+ EM A+K G L
Sbjct: 128 SGGALSKVLGEMWSKMTDDDKTQYQDMAKKDKVRYESEMKAFKDGKL 174



 Score = 56.8 bits (131), Expect = 2e-08
 Identities = 30/96 (31%), Positives = 57/96 (59%), Gaps = 5/96 (5%)

Query: 95  KARMPRSRPYNKPRGRMTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKE 154
           K R  +++  NKP+  ++AY  F+   R++ KK  P+ S    A SK   E W+ M++ +
Sbjct: 90  KKRKKQTKDPNKPKRCLSAYFHFINLKRDDVKKDNPNAS--GGALSKVLGEMWSKMTDDD 147

Query: 155 KQRFHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQ 190
           K ++ +MA++DK R++ EM+ +   KD K+  ++ +
Sbjct: 148 KTQYQDMAKKDKVRYESEMKAF---KDGKLPAKQNK 180



 Score = 55.6 bits (128), Expect = 5e-08
 Identities = 25/77 (32%), Positives = 40/77 (51%)

Query: 193 KDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSE 252
           KDPN PK + SA+  F  D+R K++    ++++ D +K    +W     E K  +   + 
Sbjct: 7   KDPNKPKGAKSAYNFFLQDQREKLQREEGKFSLADFSKVSAEKWKNMSEEEKETFVQKAG 66

Query: 253 KDKARYDREMTAYKKGP 269
           KDK R+  EM +Y   P
Sbjct: 67  KDKERFKEEMQSYTPPP 83


>SB_21059| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1024

 Score = 66.5 bits (155), Expect = 2e-11
 Identities = 29/81 (35%), Positives = 49/81 (60%)

Query: 187 RKRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAK 246
           R+R+ ++D NAPK  L+ +  F N++R KV++ +PE    ++ K LG  W+    + K +
Sbjct: 133 RRRKTLRDTNAPKAPLTGYVQFLNEQREKVRSEHPELPFPEVTKILGAEWSKMSQDDKQR 192

Query: 247 YDALSEKDKARYDREMTAYKK 267
           Y   +E+DK RY  E+  Y+K
Sbjct: 193 YLDDAERDKERYIIELENYQK 213



 Score = 58.4 bits (135), Expect = 7e-09
 Identities = 31/102 (30%), Positives = 54/102 (52%), Gaps = 2/102 (1%)

Query: 95  KARMPRSRPYNKPRGRMTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKE 154
           K R    R  N P+  +T Y  F+   RE+ + ++P++   F   +K     W+ MS+ +
Sbjct: 132 KRRRKTLRDTNAPKAPLTGYVQFLNEQREKVRSEHPELP--FPEVTKILGAEWSKMSQDD 189

Query: 155 KQRFHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMKDPN 196
           KQR+ + AE+DK R+ +E++NY      K   +K+ + K  N
Sbjct: 190 KQRYLDDAERDKERYIIELENYQKTDAYKSFVKKQIERKRKN 231


>SB_23256| Best HMM Match : HMG_box (HMM E-Value=3.3e-22)
          Length = 523

 Score = 65.7 bits (153), Expect = 4e-11
 Identities = 31/86 (36%), Positives = 50/86 (58%), Gaps = 1/86 (1%)

Query: 183 KVRGRKRQQM-KDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADP 241
           K +GRKR++  KD NAPK  L+ +  F N+ R KV++ NP+    ++ + LG  W+    
Sbjct: 161 KFKGRKRKKAHKDVNAPKAPLTGYVRFLNEHREKVRSENPDLPFHEVTRILGNMWSQLPT 220

Query: 242 ETKAKYDALSEKDKARYDREMTAYKK 267
             K  +   +EKDK RY +E+  Y++
Sbjct: 221 PQKQLFLEEAEKDKERYMKELEEYQR 246



 Score = 46.8 bits (106), Expect = 2e-05
 Identities = 26/99 (26%), Positives = 48/99 (48%), Gaps = 2/99 (2%)

Query: 95  KARMPRSRPYNKPRGRMTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKE 154
           + R    +  N P+  +T Y  F+   RE+ + + PD+   F   ++     W+ +   +
Sbjct: 165 RKRKKAHKDVNAPKAPLTGYVRFLNEHREKVRSENPDLP--FHEVTRILGNMWSQLPTPQ 222

Query: 155 KQRFHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMK 193
           KQ F E AE+DK R+  E++ Y      K+   K++ +K
Sbjct: 223 KQLFLEEAEKDKERYMKELEEYQRTDTYKMFIAKQKALK 261


>SB_7134| Best HMM Match : HMG_box (HMM E-Value=2e-16)
          Length = 228

 Score = 64.1 bits (149), Expect = 1e-10
 Identities = 31/82 (37%), Positives = 49/82 (59%), Gaps = 4/82 (4%)

Query: 188 KRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYT--MG--DIAKELGRRWAAADPET 243
           K+ + KDPNAPK+  +AFF+FC  +R+ ++  + + T  MG  ++ K L + W    P+ 
Sbjct: 45  KQDKEKDPNAPKKPANAFFMFCQQQRTVMQEDHKDATAVMGHHELTKSLAKEWNNLLPDE 104

Query: 244 KAKYDALSEKDKARYDREMTAY 265
           K  Y  + E+DK RY+ EM  Y
Sbjct: 105 KKVYYEMYERDKERYELEMKQY 126



 Score = 55.6 bits (128), Expect = 5e-08
 Identities = 32/109 (29%), Positives = 56/109 (51%), Gaps = 8/109 (7%)

Query: 94  NKARMPRSRPYNKPRGRMTAYAFFVQTCREEHKKKYPDVSVIFA--AFSKKCAERWNTMS 151
           + A+  + +  N P+    A+  F Q  R   ++ + D + +      +K  A+ WN + 
Sbjct: 42  SSAKQDKEKDPNAPKKPANAFFMFCQQQRTVMQEDHKDATAVMGHHELTKSLAKEWNNLL 101

Query: 152 EKEKQRFHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMKDPNAPKR 200
             EK+ ++EM E+DK R++LEM+ Y   K       K+Q  KDP++ KR
Sbjct: 102 PDEKKVYYEMYERDKERYELEMKQYSSDKPPS----KKQ--KDPSSKKR 144


>SB_1832| Best HMM Match : HMG_box (HMM E-Value=1.7e-22)
          Length = 299

 Score = 62.9 bits (146), Expect = 3e-10
 Identities = 38/158 (24%), Positives = 79/158 (50%), Gaps = 16/158 (10%)

Query: 105 NKPRGRMTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQ 164
           N+P+  +T+Y  + Q  R +  KKYP++       + K +++W  MSE+ K+ + E  E+
Sbjct: 150 NQPKMPLTSYFRYCQKHRAKLAKKYPNLKS--TELAAKLSKKWRKMSEERKKAYTEQYEE 207

Query: 165 DKHRFDLEMQNYVPPKDMKVRGRKRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYT 224
           +K  ++ ++ +++             + K PN  +  LSAF L+ N  R  +   NP+ +
Sbjct: 208 EKKEYETQLLDFL-------------KNKYPNV-EPPLSAFELWANQARKDLLVSNPDIS 253

Query: 225 MGDIAKELGRRWAAADPETKAKYDALSEKDKARYDREM 262
              + K+L R+W   D + K  +    + +  +Y +++
Sbjct: 254 AKKLKKKLKRKWKEIDEKGKKTWIKKEKTEMRKYQKKI 291



 Score = 52.4 bits (120), Expect = 4e-07
 Identities = 28/119 (23%), Positives = 56/119 (47%), Gaps = 2/119 (1%)

Query: 151 SEKEKQRFHEMAEQ-DKHRFDLEM-QNYVPPKDMKVRGRKRQQMKDPNAPKRSLSAFFLF 208
           +E+ + +FH + +   K R   E+ ++   P       +  +   DPN PK  L+++F +
Sbjct: 103 NEEIQTKFHRLCQSVRKMRTAREVIEDIKVPSHANRSPKTPKPFVDPNQPKMPLTSYFRY 162

Query: 209 CNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARYDREMTAYKK 267
           C   R+K+    P     ++A +L ++W     E K  Y    E++K  Y+ ++  + K
Sbjct: 163 CQKHRAKLAKKYPNLKSTELAAKLSKKWRKMSEERKKAYTEQYEEEKKEYETQLLDFLK 221


>SB_37758| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 382

 Score = 61.3 bits (142), Expect = 9e-10
 Identities = 35/102 (34%), Positives = 54/102 (52%), Gaps = 6/102 (5%)

Query: 172 EMQNYVPPKDMK---VRGRKRQQMKDPNAPKRSLSAFF---LFCNDERSKVKAGNPEYTM 225
           ++Q    PK  K    +G+KR++  D N PK  ++ +     F N  R  VK  +P  T 
Sbjct: 165 QIQTIAEPKKRKGGWPKGKKRKKEYDLNKPKAPVTGYVHYVRFLNSRRESVKHQHPHLTF 224

Query: 226 GDIAKELGRRWAAADPETKAKYDALSEKDKARYDREMTAYKK 267
            +I K LG+ W +  PE K K+   +E+DK RY  E+ AY++
Sbjct: 225 PEITKMLGQEWNSLLPEEKQKFLDEAEEDKKRYVEELRAYQQ 266



 Score = 51.2 bits (117), Expect = 1e-06
 Identities = 30/97 (30%), Positives = 53/97 (54%), Gaps = 6/97 (6%)

Query: 105 NKPRGRMTAYAFFVQ---TCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEM 161
           NKP+  +T Y  +V+   + RE  K ++P ++  F   +K   + WN++  +EKQ+F + 
Sbjct: 192 NKPKAPVTGYVHYVRFLNSRRESVKHQHPHLT--FPEITKMLGQEWNSLLPEEKQKFLDE 249

Query: 162 AEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMKDPNAP 198
           AE+DK R+  E++ Y   +  +    KRQ +K    P
Sbjct: 250 AEEDKKRYVEELRAYQQSEQYQA-FVKRQHVKRTKHP 285


>SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)
          Length = 783

 Score = 59.7 bits (138), Expect = 3e-09
 Identities = 38/149 (25%), Positives = 79/149 (53%), Gaps = 7/149 (4%)

Query: 119 QTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQDKHRFDLEMQNYVP 178
           Q  REE + + P  S    + S+  +++      ++K++  +  +  K +   +  ++V 
Sbjct: 468 QDVREEFESE-PSSSEASGSDSEGESKKKKKEKPEKKRKAEKPPKSPKKK--AKTASFVE 524

Query: 179 PKDMKVRGRKRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAA 238
             +   +G K++  KDPNAPKR++SA+ L+ ND R ++K  NP  ++ +++K  G  W  
Sbjct: 525 TGEKGKKGGKKK--KDPNAPKRAMSAYMLWLNDTRQEIKDKNPGISVTEVSKVAGEMW-- 580

Query: 239 ADPETKAKYDALSEKDKARYDREMTAYKK 267
            +   K+K++  +  +K +Y + M  Y +
Sbjct: 581 KNLTDKSKWEEKAAIEKQKYVQRMKEYNE 609



 Score = 44.8 bits (101), Expect = 9e-05
 Identities = 35/127 (27%), Positives = 54/127 (42%), Gaps = 8/127 (6%)

Query: 105 NKPRGRMTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQ 164
           N P+  M+AY  ++   R+E K K P +SV     SK   E W  +++K K  + E A  
Sbjct: 540 NAPKRAMSAYMLWLNDTRQEIKDKNPGISV--TEVSKVAGEMWKNLTDKSK--WEEKAAI 595

Query: 165 DKHRFDLEMQNY----VPPKDMKVRGRKRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGN 220
           +K ++   M+ Y       K+     +K    K   APK   S+      D   K K+  
Sbjct: 596 EKQKYVQRMKEYNENKKNDKEESSPKKKSPSKKASKAPKEEKSSSPRKSLDNNQKFKSAE 655

Query: 221 PEYTMGD 227
              + GD
Sbjct: 656 FVESSGD 662


>SB_16438| Best HMM Match : HMG_box (HMM E-Value=7.2e-31)
          Length = 690

 Score = 51.2 bits (117), Expect = 1e-06
 Identities = 36/128 (28%), Positives = 57/128 (44%), Gaps = 14/128 (10%)

Query: 140 SKKCAERWNTMSEKEKQRFHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMKDPNAPK 199
           +K   E W  +++++K+ +   AE DK R+            +K  G K    KDP+ PK
Sbjct: 532 AKLAGEEWKKLNDEQKKPYVAKAEADKQRY------------LKESG-KNDPKKDPDKPK 578

Query: 200 RSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARYD 259
           R  +A+FLF    R K  AG        I    G RW     E K  Y     +++ +Y+
Sbjct: 579 RPPTAYFLFLAAFR-KEMAGKALEDGKKIPSLAGERWREMSDEDKKPYTIQEAEERNKYE 637

Query: 260 REMTAYKK 267
           + M  ++K
Sbjct: 638 KVMEEWRK 645



 Score = 48.0 bits (109), Expect = 9e-06
 Identities = 29/80 (36%), Positives = 41/80 (51%), Gaps = 5/80 (6%)

Query: 187 RKRQQMKDPNAPKRSLSAFFLFCNDERSKVKA-----GNPEYTMGDIAKELGRRWAAADP 241
           RK++   D    KR+ SA+  F +D R+K+KA     G P     ++AK  G  W   + 
Sbjct: 485 RKKKAKGDGPVVKRASSAYIHFTSDFRAKLKAKSAKSGTPLPKANEVAKLAGEEWKKLND 544

Query: 242 ETKAKYDALSEKDKARYDRE 261
           E K  Y A +E DK RY +E
Sbjct: 545 EQKKPYVAKAEADKQRYLKE 564


>SB_18968| Best HMM Match : HMG_box (HMM E-Value=1.2e-19)
          Length = 1204

 Score = 49.6 bits (113), Expect = 3e-06
 Identities = 26/84 (30%), Positives = 42/84 (50%), Gaps = 1/84 (1%)

Query: 180 KDMKVRGRKRQQM-KDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAA 238
           K  + +G KR++  K    P + LSA+ +F  + ++ ++  NP    G+IAK +G+ W  
Sbjct: 906 KKKRKKGEKRKRKTKIEGEPPKPLSAYQIFFKETQAAIRLQNPSAQFGEIAKIVGQMWEN 965

Query: 239 ADPETKAKYDALSEKDKARYDREM 262
              E K  Y    E  KA Y + M
Sbjct: 966 LPEEQKKVYHCKHETAKAEYQKAM 989


>SB_42643| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 557

 Score = 47.6 bits (108), Expect = 1e-05
 Identities = 24/91 (26%), Positives = 44/91 (48%)

Query: 179 PKDMKVRGRKRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAA 238
           P D ++       +K     KR ++AF ++  +ER K+   NP+    +I+K LG  W  
Sbjct: 308 PDDQEINASLMSLVKPVEHVKRPMNAFMVWSREERRKIAQENPKMHNSEISKRLGSEWKQ 367

Query: 239 ADPETKAKYDALSEKDKARYDREMTAYKKGP 269
              + K  +   ++K +A++ +E   YK  P
Sbjct: 368 LADDDKKPFVEEAKKLRAQHMKEHPDYKYRP 398


>SB_12182| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1361

 Score = 44.8 bits (101), Expect = 9e-05
 Identities = 18/64 (28%), Positives = 36/64 (56%)

Query: 199  KRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARY 258
            KR  S + LF ++ R  ++  +PEY  G+I++ +G  W  A    KA+Y+  ++   ++ 
Sbjct: 1269 KRGQSGYLLFSHEMRGIIRKEHPEYAFGEISRLIGEEWRNASAARKAEYENKAQMQLSQL 1328

Query: 259  DREM 262
            + E+
Sbjct: 1329 ETEV 1332


>SB_46509| Best HMM Match : HMG_box (HMM E-Value=4.2e-10)
          Length = 145

 Score = 44.4 bits (100), Expect = 1e-04
 Identities = 18/63 (28%), Positives = 35/63 (55%)

Query: 199 KRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARY 258
           KR  S + LF ++ R  ++  +PEY  G+I++ +G  W  A    KA+Y+  ++   ++ 
Sbjct: 36  KRGQSGYLLFSHEMRGIIRKEHPEYAFGEISRLIGEEWRNASAARKAEYENKAQMQLSQL 95

Query: 259 DRE 261
           + E
Sbjct: 96  ETE 98


>SB_33348| Best HMM Match : HMG_box (HMM E-Value=3.2e-34)
          Length = 179

 Score = 44.0 bits (99), Expect = 2e-04
 Identities = 21/71 (29%), Positives = 38/71 (53%)

Query: 199 KRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARY 258
           KR ++AF ++  +ER K+   NP+    +I+K LG  W     + K  +   ++K +A++
Sbjct: 11  KRPMNAFMVWSREERRKIAQENPKMHNSEISKRLGSEWKQLADDDKKPFVEEAKKLRAQH 70

Query: 259 DREMTAYKKGP 269
            +E   YK  P
Sbjct: 71  MKEHPDYKYRP 81


>SB_31139| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 315

 Score = 43.6 bits (98), Expect = 2e-04
 Identities = 26/78 (33%), Positives = 42/78 (53%), Gaps = 2/78 (2%)

Query: 193 KDPNAPKRSLSAFFLFCNDERSKVKAGN-PEYTMGDIAKELGRRWAAADPETKAKYDALS 251
           KD N  KR ++AF ++ + ER ++K+   P     +I+K LG  W A   E K  Y   +
Sbjct: 4   KDANHVKRPMNAFMVW-SKERRRIKSQECPRMHNSEISKILGCEWKAMKDELKQPYIEKA 62

Query: 252 EKDKARYDREMTAYKKGP 269
           ++ +A++ RE   YK  P
Sbjct: 63  KELQAQHSRENPGYKYKP 80


>SB_23680| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 548

 Score = 43.2 bits (97), Expect = 3e-04
 Identities = 26/75 (34%), Positives = 32/75 (42%), Gaps = 3/75 (4%)

Query: 195 PNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKD 254
           P   KR   AFF F  D   KVK  NP     +I K L  +WA  +P  K  Y    ++D
Sbjct: 303 PKKHKRPTPAFFRFRQDYADKVKEENPHLKDAEIRKHLSDQWANLEPSVKECYRKEYQQD 362

Query: 255 KARYDREMTAYKKGP 269
              +      YKK P
Sbjct: 363 MEEF---RAPYKKLP 374



 Score = 39.9 bits (89), Expect = 0.002
 Identities = 32/119 (26%), Positives = 52/119 (43%), Gaps = 6/119 (5%)

Query: 153 KEKQRFHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMKDPNAPKRSLSA-----FFL 207
           KE+    + AE  KH  D +  N  P      R   +Q M++  AP + L       F L
Sbjct: 325 KEENPHLKDAEIRKHLSD-QWANLEPSVKECYRKEYQQDMEEFRAPYKKLPRKPPRHFSL 383

Query: 208 FCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARYDREMTAYK 266
           F  +  +K+KA N   +  DI +ELG +W       + +    SE  +  Y+ E+  ++
Sbjct: 384 FMRENFAKIKAENQGMSNPDIMRELGAKWRNLPFSEREEIRRKSEDAQKTYEAEIVEFE 442



 Score = 34.3 bits (75), Expect = 0.12
 Identities = 37/180 (20%), Positives = 72/180 (40%), Gaps = 20/180 (11%)

Query: 103 PYNK-PRGRMTAYAFFVQTCREEHKK-KYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHE 160
           PY K PR     ++ F+   RE   K K  +  +      ++   +W  +   E++    
Sbjct: 369 PYKKLPRKPPRHFSLFM---RENFAKIKAENQGMSNPDIMRELGAKWRNLPFSEREEIRR 425

Query: 161 MAEQDKHRFDLEMQNY-------------VPPKDMKVRGRKRQQMKDPNAPKRSLSAFFL 207
            +E  +  ++ E+  +             V  KD K++  K+++MK  N PK+  +AF  
Sbjct: 426 KSEDAQKTYEAEIVEFEDQLSEGEKEFLNVQAKD-KMKELKQRKMKLLNFPKKPGTAFIY 484

Query: 208 FCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARYDREMTAYKK 267
           F   ER        E  +    + +   W     + K  Y   + +   +Y+ E+T +K+
Sbjct: 485 FLTMERESFPRREGE-GITQWTQRMAETWRDMSDDEKEMYRMANRRAVEKYNEEVTEWKE 543


>SB_29734| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 304

 Score = 41.9 bits (94), Expect = 6e-04
 Identities = 22/80 (27%), Positives = 39/80 (48%)

Query: 190 QQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDA 249
           ++  D    KR ++AF ++   ER K+   +P+    +I+K LG+RW       K  +  
Sbjct: 37  KKKSDMQHVKRPMNAFMVWSQIERRKMAEEHPDMHNAEISKRLGKRWKLLSESEKRPFVE 96

Query: 250 LSEKDKARYDREMTAYKKGP 269
            SE+ + R+ +    YK  P
Sbjct: 97  ESERLRIRHMQAYPDYKYRP 116



 Score = 39.9 bits (89), Expect = 0.002
 Identities = 28/81 (34%), Positives = 44/81 (54%), Gaps = 8/81 (9%)

Query: 111 MTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQDKHRFD 170
           M A+  + Q  R +  +++PD+    A  SK+  +RW  +SE EK+ F E +E+ + R  
Sbjct: 49  MNAFMVWSQIERRKMAEEHPDMHN--AEISKRLGKRWKLLSESEKRPFVEESERLRIR-- 104

Query: 171 LEMQNYVPPKDMKVRGRKRQQ 191
             MQ Y    D K R RK++Q
Sbjct: 105 -HMQAY---PDYKYRPRKKKQ 121


>SB_16422| Best HMM Match : HMG_box (HMM E-Value=8.7e-26)
          Length = 245

 Score = 41.1 bits (92), Expect = 0.001
 Identities = 23/71 (32%), Positives = 33/71 (46%)

Query: 199 KRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARY 258
           KR L+AF L+    R  +   NP+    DI+++LG  W     E KA Y   ++K K  +
Sbjct: 20  KRPLNAFILWSKKRRRVIANENPQMHNFDISRKLGLEWQKLTEEEKAYYFEEAKKLKEEH 79

Query: 259 DREMTAYKKGP 269
                 YK  P
Sbjct: 80  KERYPHYKYQP 90


>SB_13764| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1099

 Score = 41.1 bits (92), Expect = 0.001
 Identities = 21/84 (25%), Positives = 39/84 (46%)

Query: 186 GRKRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKA 245
           G +  +    +  KR ++AF ++  + R K+   NP+    +I+K LG  W     + K 
Sbjct: 777 GNESTKANSADRVKRPMNAFMVWSRERRRKMAQDNPKMHNSEISKRLGSEWKLLSEQEKR 836

Query: 246 KYDALSEKDKARYDREMTAYKKGP 269
            Y   + + +A + +E   YK  P
Sbjct: 837 PYIDEARRLRAVHMKEHPDYKYRP 860


>SB_3516| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 642

 Score = 40.7 bits (91), Expect = 0.001
 Identities = 23/85 (27%), Positives = 42/85 (49%), Gaps = 1/85 (1%)

Query: 186 GRKRQQMKDPNAP-KRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETK 244
           GR R +  D     KR ++AF ++   ER ++   NPE    +++K LG  W A +   K
Sbjct: 353 GRARTEKDDETERIKRPMNAFMVWAQVERRRLADANPELHNAELSKMLGLTWRALNSTQK 412

Query: 245 AKYDALSEKDKARYDREMTAYKKGP 269
             +   +E+ + ++ ++   YK  P
Sbjct: 413 RPFVDEAERLRLQHMQDYPNYKYRP 437


>SB_29576| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1202

 Score = 39.1 bits (87), Expect = 0.004
 Identities = 19/57 (33%), Positives = 30/57 (52%)

Query: 199 KRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDK 255
           +R ++AF +F    R+ V   +P      ++K LG  W A  PE K  Y+AL+ + K
Sbjct: 517 RRPMNAFMIFSKRHRALVHQKHPHQDNRTVSKILGEWWYALGPEEKQSYNALAAQVK 573


>SB_53304| Best HMM Match : HMG_box (HMM E-Value=1.9e-32)
          Length = 398

 Score = 38.3 bits (85), Expect = 0.007
 Identities = 24/91 (26%), Positives = 44/91 (48%), Gaps = 4/91 (4%)

Query: 179 PKDMKVRGRKRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAA 238
           P  ++V G K Q+   P+  KR ++AF ++    R K+    P     +++K LG+ W  
Sbjct: 49  PLPVRVNGIKTQK---PHV-KRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWKL 104

Query: 239 ADPETKAKYDALSEKDKARYDREMTAYKKGP 269
            +   K  +   +E+ + ++ RE   YK  P
Sbjct: 105 LNDSEKKPFIEEAERLRIKHKREHPDYKYQP 135


>SB_26162| Best HMM Match : HMG_box (HMM E-Value=1.5e-31)
          Length = 367

 Score = 37.1 bits (82), Expect = 0.017
 Identities = 19/87 (21%), Positives = 42/87 (48%)

Query: 183 KVRGRKRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPE 242
           ++   + +  +D +  KR +++F ++    R K    NP+    +I+K LG+ W     +
Sbjct: 79  RLERHRYEDEEDDSHVKRPMNSFMIWAKVMRRKFAEENPKLHNAEISKLLGKAWNELTTK 138

Query: 243 TKAKYDALSEKDKARYDREMTAYKKGP 269
            K  +   +E+ + R+ +E   Y+  P
Sbjct: 139 DKRPFVEKAERLRIRHMKEHPNYRYTP 165



 Score = 31.5 bits (68), Expect = 0.86
 Identities = 22/82 (26%), Positives = 39/82 (47%), Gaps = 3/82 (3%)

Query: 111 MTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQDKHRFD 170
           M ++  + +  R +  ++ P +    A  SK   + WN ++ K+K+ F E AE+ + R  
Sbjct: 98  MNSFMIWAKVMRRKFAEENPKLHN--AEISKLLGKAWNELTTKDKRPFVEKAERLRIRHM 155

Query: 171 LEMQNY-VPPKDMKVRGRKRQQ 191
            E  NY   PK    + R+  Q
Sbjct: 156 KEHPNYRYTPKRRGRQDRRNGQ 177


>SB_25642| Best HMM Match : HMG_box (HMM E-Value=1.3e-29)
          Length = 267

 Score = 36.7 bits (81), Expect = 0.023
 Identities = 21/71 (29%), Positives = 35/71 (49%)

Query: 199 KRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARY 258
           KR ++AF ++ + +R ++ A NP+     I+K LG  W     E K K+ A ++     +
Sbjct: 8   KRPMNAFMIWSSKKRRQLAAENPKLHNSQISKMLGTEWRKLTVEEKQKFFAEAKLLNELH 67

Query: 259 DREMTAYKKGP 269
             E   YK  P
Sbjct: 68  MIEHPDYKYRP 78


>SB_10678| Best HMM Match : HMG_box (HMM E-Value=1.3e-29)
          Length = 494

 Score = 36.7 bits (81), Expect = 0.023
 Identities = 21/71 (29%), Positives = 35/71 (49%)

Query: 199 KRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARY 258
           KR ++AF ++ + +R ++ A NP+     I+K LG  W     E K K+ A ++     +
Sbjct: 8   KRPMNAFMIWSSKKRRQLAAENPKLHNSQISKMLGTEWRKLTVEEKQKFFAEAKLLNELH 67

Query: 259 DREMTAYKKGP 269
             E   YK  P
Sbjct: 68  MIEHPDYKYRP 78


>SB_2908| Best HMM Match : HMG_box (HMM E-Value=1.5e-08)
          Length = 324

 Score = 34.7 bits (76), Expect = 0.092
 Identities = 15/52 (28%), Positives = 25/52 (48%)

Query: 193 KDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETK 244
           K P  P++ L  +  +      +VK  NP++ + DI K +G+ W   D   K
Sbjct: 23  KPPKPPEKPLMPYMRYSRKVWDQVKNQNPDFKLWDIGKIIGQMWRDLDDAEK 74


>SB_27742| Best HMM Match : HMG_box (HMM E-Value=1.3e-24)
          Length = 201

 Score = 33.1 bits (72), Expect = 0.28
 Identities = 17/71 (23%), Positives = 34/71 (47%)

Query: 199 KRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARY 258
           KR ++AF ++   ER K+    P     +I+K LG  W+    E K  +   +++ +  +
Sbjct: 10  KRPMNAFMVWSRTERRKLALKYPNMLNCEISKLLGAEWSRLSEEEKRPFVTEAKRLRTIH 69

Query: 259 DREMTAYKKGP 269
           +++   Y   P
Sbjct: 70  NQKYPDYSYKP 80



 Score = 31.5 bits (68), Expect = 0.86
 Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 6/83 (7%)

Query: 111 MTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQDKHRFD 170
           M A+  + +T R +   KYP++  +    SK     W+ +SE+EK+ F     + K    
Sbjct: 13  MNAFMVWSRTERRKLALKYPNM--LNCEISKLLGAEWSRLSEEEKRPF---VTEAKRLRT 67

Query: 171 LEMQNYVPPKDMKVRGRKRQQMK 193
           +  Q Y P    K R RK + +K
Sbjct: 68  IHNQKY-PDYSYKPRRRKSKTVK 89


>SB_52386| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 384

 Score = 32.7 bits (71), Expect = 0.37
 Identities = 24/93 (25%), Positives = 44/93 (47%), Gaps = 6/93 (6%)

Query: 106 KPRGRMTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQD 165
           K +  M A+  + +  R    K+YP  +   A  S +  E WN +S ++++ + + A + 
Sbjct: 100 KVKRPMNAFMIWARLHRSTIAKRYPQANN--AEISIRLGEIWNDLSSEQQKPYFDEATRL 157

Query: 166 KHRFDLEMQNYV-PPKDMKVRGRKRQQMKDPNA 197
           K +   E  N+V  P+  K   R+  Q+  P A
Sbjct: 158 KDKHKAEHPNWVYQPRPAK---RRALQLGTPGA 187


>SB_49511| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 157

 Score = 32.7 bits (71), Expect = 0.37
 Identities = 18/43 (41%), Positives = 27/43 (62%), Gaps = 6/43 (13%)

Query: 158 FHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMKDPNAPKR 200
           ++EM E+DK R++LEM+ Y   K       K+Q  KDP++ KR
Sbjct: 37  YYEMYERDKERYELEMKQYSSDKPPS----KKQ--KDPSSKKR 73


>SB_41600| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 416

 Score = 31.9 bits (69), Expect = 0.65
 Identities = 26/95 (27%), Positives = 45/95 (47%), Gaps = 4/95 (4%)

Query: 150 MSEKEKQRFHEM-AEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMK-DPNAPKRSLSAFFL 207
           MS + + ++H    E D H+    + + +   D  +   K +  K  PN P  S+S  F+
Sbjct: 1   MSGRAQDKWHRHNKEDDIHKRGEVLCSVLEICDWSIEAAKHRNSKLPPNLPCSSIS--FV 58

Query: 208 FCNDERSKVKAGNPEYTMGDIAKELGRRWAAADPE 242
           F   +R+     +P  T+  +AKELG   +A + E
Sbjct: 59  FAKWDRTLTGKYHPRSTVWKMAKELGLDASAYERE 93


>SB_3226| Best HMM Match : RNA_pol_Rpb2_1 (HMM E-Value=0.021)
          Length = 1217

 Score = 30.3 bits (65), Expect = 2.0
 Identities = 19/78 (24%), Positives = 41/78 (52%), Gaps = 4/78 (5%)

Query: 117 FVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQDKHRFDLEMQNY 176
           +++T R+EHK      +VI A   K+  E  + M E+E++ + ++ +Q     + E +  
Sbjct: 504 YLRTARKEHKLMLERKTVIEAR--KEYLE--SVMQERERREYEKIKKQKVENQEAEQKRL 559

Query: 177 VPPKDMKVRGRKRQQMKD 194
              +  +   R+RQ+++D
Sbjct: 560 DEERRQREIQRRRQELQD 577


>SB_6714| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 233

 Score = 29.9 bits (64), Expect = 2.6
 Identities = 28/112 (25%), Positives = 49/112 (43%), Gaps = 9/112 (8%)

Query: 152 EKEKQRFHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQQM-KDPNAPKRSLSAFFLFCN 210
           +KE+    EM ++DK   D E +  +  ++M      +++M K+    K  +    +   
Sbjct: 55  DKEEMDQEEMDKEDKEEMDKEDKEEMDQEEMDKEEMDQEEMDKEDKEDKEEMDQEEM--- 111

Query: 211 DERSKVKAGNPEYTMGDIAKELGRRWAAADPETKAKYDALSEKDKARYDREM 262
           D+  K +    E    D  +E+G+     D E K + D   E DK   D+EM
Sbjct: 112 DKEDKEEMDKEEMDKED-KEEMGK--GEMDKEDKEEMD--QEMDKEEMDQEM 158


>SB_32415| Best HMM Match : Gag_MA (HMM E-Value=3.2)
          Length = 299

 Score = 29.5 bits (63), Expect = 3.5
 Identities = 26/131 (19%), Positives = 53/131 (40%), Gaps = 12/131 (9%)

Query: 127 KKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAE---QDKHRFDLEMQNYVPPKDMK 183
           K++P +   F  ++ +  + W TM E+E+Q+  +  +   +D  RF  ++     PKD  
Sbjct: 105 KEFPPLGFDFDRYTPESNKDWATMVEEEEQQKDQPTQITLKDPSRFKRKLL-LTDPKDQP 163

Query: 184 VRGRKRQQMKDPNAPKRSLSAFFLFCNDERSKVKAGNPEYTMGDIAKELGR-RWAAADPE 242
              +       P    +S+          + ++  G      G  + ++ R + A  DP 
Sbjct: 164 TSNK-------PTVSNKSMETDLHKLEKRQKQIDIGKNTVAYGRFSAQIPREKRAKEDPS 216

Query: 243 TKAKYDALSEK 253
           T  K+   S +
Sbjct: 217 TPDKFQQCSTR 227


>SB_29054| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 515

 Score = 28.7 bits (61), Expect = 6.0
 Identities = 29/119 (24%), Positives = 56/119 (47%), Gaps = 10/119 (8%)

Query: 153 KEKQRFHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMKDPNAPKRSLSAFFLFCNDE 212
           KE Q F    ++++   D E++ +V  K + +  +K      P   +  LSAF    N  
Sbjct: 232 KELQEFERKKQEEE---DEEIRQFVYAKKVVIYTKKNTNAYLPK--QFLLSAFQDHTNRM 286

Query: 213 RSKVKAGNPEYTMGD----IAKELGRRWAAADPETKAKYDALSEKDKARYDREMTAYKK 267
           R K+ A   +  + D    IAK L  R A  + E +AK++A  ++ +  ++  ++  ++
Sbjct: 287 RQKL-ADQMQQKVDDEDERIAKALAEREAKREAEERAKFEADMKEKRGIHEHRISMMRE 344


>SB_26233| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 463

 Score = 28.7 bits (61), Expect = 6.0
 Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 3/65 (4%)

Query: 152 EKEKQRFHEMAEQDKHRFDLEMQNYVPPKDMKVRGRKRQQMKDPNAPKRSLSAFFLFCND 211
           E+EK+R  +  E+ K R + E Q +   ++ K + R+R++ K     ++ L    L    
Sbjct: 332 EREKERIQQEKEKQKERREKEKQKH---QEKKEKERQREKEKLEKEKQKELERKELQRQK 388

Query: 212 ERSKV 216
           ER K+
Sbjct: 389 ERMKI 393


>SB_23345| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 903

 Score = 28.7 bits (61), Expect = 6.0
 Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 4/58 (6%)

Query: 111 MTAYAFFVQTCREEHKKKYPDVSVIFAAFSKKCAERWNTMSEKEKQRFHEMAEQDKHR 168
           ++A  FF Q  +EE KK+  D   +F    KK     + + +K+K++ H+  +  +H+
Sbjct: 342 ISAVKFFEQKQKEEFKKEETDYLDVF----KKGGTTKDKIEKKDKKKHHKKHKDKEHK 395


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.130    0.391 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,485,937
Number of Sequences: 59808
Number of extensions: 253546
Number of successful extensions: 1001
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 930
Number of HSP's gapped (non-prelim): 65
length of query: 297
length of database: 16,821,457
effective HSP length: 82
effective length of query: 215
effective length of database: 11,917,201
effective search space: 2562198215
effective search space used: 2562198215
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 60 (28.3 bits)

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