BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001131-TA|BGIBMGA001131-PA|IPR001247|Exoribonuclease (375 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC757.08 |||exosome subunit Rrp45 |Schizosaccharomyces pombe|c... 202 8e-53 SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces pombe... 93 8e-20 SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 35 0.016 SPAC3G9.10c |ski6||exosome subunit Ski6 |Schizosaccharomyces pom... 35 0.022 SPCC1494.08c |||conserved fungal protein|Schizosaccharomyces pom... 31 0.20 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 30 0.61 SPAC30D11.03 |drs1||ATP-dependent RNA helicase Drs1 |Schizosacch... 29 0.81 SPBC1347.02 |fkbp39||FKBP-type peptidyl-prolyl cis-trans isomera... 29 1.1 SPBC3E7.07c |||DUF757 family protein|Schizosaccharomyces pombe|c... 29 1.1 SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 28 1.9 SPBC6B1.09c |nbs1||Mre11 complex subunit Nbs1|Schizosaccharomyce... 28 2.5 SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch... 28 2.5 SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb... 28 2.5 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 2.5 SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pom... 27 4.3 SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosac... 27 5.7 SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 27 5.7 SPAC26F1.09 |gyp51||GTPase activating protein Gyp51 |Schizosacch... 27 5.7 SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces... 27 5.7 SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 26 7.5 SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo... 26 10.0 SPAC683.02c ||SPAC694.01c|zf-CCHC type zinc finger protein|Schiz... 26 10.0 SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces po... 26 10.0 >SPCC757.08 |||exosome subunit Rrp45 |Schizosaccharomyces pombe|chr 3|||Manual Length = 291 Score = 202 bits (492), Expect = 8e-53 Identities = 101/287 (35%), Positives = 164/287 (57%), Gaps = 16/287 (5%) Query: 3 DTCLSNCEKNFIQKIVSQGHRLDGRNFNETRKLDISFGSEYGCCIVSLGETKILSEVTCE 62 +T L+N K F+ + +G RLDGR ++ R L+I FG EYG VS G T++++ +T E Sbjct: 6 ETSLNN--KEFVLNSLEKGLRLDGRQLSDFRSLEIQFGKEYGQVDVSFGHTRVMARITTE 63 Query: 63 VAQPKQIRPNEGIIFMNVELNPMAAPQFEANRQTDLTVYLVRLLEKCYKDSKCIDLESLC 122 + +P RP +GI + EL P+A FEA R +D + + RL+EK + S +D ESLC Sbjct: 64 ITKPYTDRPFDGIFSITTELTPLAYSAFEAGRVSDQEIVISRLIEKAVRRSNALDTESLC 123 Query: 123 IVVEEKVWSLRINIKVLNHDGNLTECASIATLASIAHFRRPDFTRDGDRVIIHTLTEKDP 182 I+ +K W +R ++ +NHDGNL + A IA +A++ HFRRP+ T G+ V +H + E+ P Sbjct: 124 IISGQKCWHVRASVHFINHDGNLVDAACIAVIAALCHFRRPELTVVGEEVTVHPVEERVP 183 Query: 183 IPTVLYHYPVCTTFALYSN-DILLTDPNFLEESVCTSNTEEGINTAGGLLVLGMNQYREV 241 +P + H P+C TF+ ++N ++ + D EE +C G + + +N+ REV Sbjct: 184 VPLSILHMPICVTFSFFNNGELAIVDATLEEEDLC-----------NGSMTITLNKNREV 232 Query: 242 CLLDLTGAAIYTSNIVHKALLSAADKCKKYVEIIKNAVAKDDNLRQK 288 C + G I + + +A D +I+ A+ D++LR+K Sbjct: 233 CQIYKAGGIIIDPSKIISCAKTAFDIAVSVCSVIQQAL--DEDLRKK 277 >SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces pombe|chr 2|||Manual Length = 270 Score = 92.7 bits (220), Expect = 8e-20 Identities = 63/213 (29%), Positives = 113/213 (53%), Gaps = 11/213 (5%) Query: 11 KNFIQKIVSQGHRLDGRNFNETRKLDIS---FGSEYGCCIVSLGETKILSEVTCEVAQPK 67 + ++ +++Q R DGR+ +E R++ I+ + G I+ GE + + E+A+P Sbjct: 23 EQYLSHLLNQDVRSDGRSVSEFREIVINDNCISTANGSAIIRAGENVFVCGIKAEIAEPF 82 Query: 68 QIRPNEGIIFMNVELNPMAAPQFEANRQTDLTVYLVRLLEKCYKDSKCIDLESLCIVVEE 127 + PNEG I N+EL+P+ + +F+ +DL + + L + + S I+L+SLCI ++ Sbjct: 83 ENSPNEGWIVPNLELSPLCSSKFKPGPPSDLAQVVSQELHQTLQQSNLINLQSLCIFEKK 142 Query: 128 KVWSLRINIKVLNHDGNLTECASIATLASIAHFRRPDFTRDGD--RVI-IHTLTEKDPIP 184 W L +I LN+DG+ + A A A++ + P D D RVI TLT + Sbjct: 143 AAWVLYADIICLNYDGSAFDYAWAALFAALKTVKLPTAVWDEDLERVICASTLTRPVQLS 202 Query: 185 TVLYHYPVCTTFALYSNDILLTDPNFLEESVCT 217 T + + +++++ +D LL DP EE + T Sbjct: 203 TEVRSF----SWSVF-DDKLLADPTDEEEDLST 230 >SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 817 Score = 35.1 bits (77), Expect = 0.016 Identities = 28/100 (28%), Positives = 43/100 (43%), Gaps = 4/100 (4%) Query: 196 FALYSNDILLTDPNFLEESVCTSNTEEGIN-TAGGLLVLG--MNQYREVCLLDLTGAAIY 252 F LY N L L E C NTE+ + GG M +Y V + +T A+ Sbjct: 573 FLLYENHFLEIFNRHLTEGDCNRNTEKDVKCVRGGFFSYSSKMYKYDRVSIPVITRASSS 632 Query: 253 TSNIVHKALLSAADKCKKYVEIIKNAVAKDDNLRQKREKI 292 + ++ L +K KY I K +A+ NL + +K+ Sbjct: 633 RNLLITMNCLRVLEKVCKYSNIRKEIIAR-SNLHENLKKL 671 >SPAC3G9.10c |ski6||exosome subunit Ski6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 242 Score = 34.7 bits (76), Expect = 0.022 Identities = 22/67 (32%), Positives = 31/67 (46%), Gaps = 3/67 (4%) Query: 20 QGHRLDGRNFNETRKLDISFG---SEYGCCIVSLGETKILSEVTCEVAQPKQIRPNEGII 76 +G R DGR ++E R G SE G + LG TK+L V + + Sbjct: 10 EGLRNDGRRWDEMRNFQCRIGIEPSENGSAFIELGNTKVLCIVDGPSEPVIKSKARADRT 69 Query: 77 FMNVELN 83 F+NVE+N Sbjct: 70 FVNVEIN 76 >SPCC1494.08c |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 274 Score = 31.5 bits (68), Expect = 0.20 Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 4/64 (6%) Query: 178 TEKDPI--PTVLYHYPVCTTFALY-SNDILLTDPNFLEESVCTSNTEEGINTAGGLLVLG 234 TE +P+ V + Y C + + ND LL D + + +C S+T+ G+ G+L + Sbjct: 50 TEAEPVLEHRVTWEYETCVSVRMIIDNDNLLKDKFLILQVLCDSHTDSGVIRL-GILKIN 108 Query: 235 MNQY 238 + +Y Sbjct: 109 LTEY 112 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 29.9 bits (64), Expect = 0.61 Identities = 20/86 (23%), Positives = 43/86 (50%), Gaps = 4/86 (4%) Query: 257 VHKALLSAADKCKKYVEIIKNAVAKDDNLRQK----REKINFSDIITTEHMLALSRKDLN 312 +++ + +AAD+ I KN + +QK +EKINF + + EH +S + + Sbjct: 832 LNERISAAADELSSMESINKNQANELKLAKQKCSNLQEKINFGNKLAKEHTEKISSLEKD 891 Query: 313 IQLKHYDVTELDEKDEEMFSDEDDIK 338 ++ + L ++ + + S+ D +K Sbjct: 892 LEAATKTASTLSKELKTVKSENDSLK 917 >SPAC30D11.03 |drs1||ATP-dependent RNA helicase Drs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 754 Score = 29.5 bits (63), Expect = 0.81 Identities = 17/66 (25%), Positives = 33/66 (50%), Gaps = 3/66 (4%) Query: 310 DLNIQLKHYDVTELDEKDEEMFSDEDDIKPEPSKYDVVATVPNTAELKSKEGKSEWIEVS 369 D ++ + + + E ++E DE + + E D+ VP+ A + +++ S+ S Sbjct: 171 DEDLAIDGFGAGAIAENEDESSQDESESEEED---DITEPVPSFANISTQDFNSDSAAGS 227 Query: 370 SDSGED 375 SDS ED Sbjct: 228 SDSEED 233 >SPBC1347.02 |fkbp39||FKBP-type peptidyl-prolyl cis-trans isomerase|Schizosaccharomyces pombe|chr 2|||Manual Length = 361 Score = 29.1 bits (62), Expect = 1.1 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%) Query: 323 LDEKDEEMFSDEDDIKPEPSKYDVVATVPNTAELKSKEGKSE 364 +DE+DEE ++D P++ D+V TV E +E +SE Sbjct: 123 VDEEDEEEEESDEDYDLSPTEEDLVETVSGDEE-SEEESESE 163 >SPBC3E7.07c |||DUF757 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 29.1 bits (62), Expect = 1.1 Identities = 16/53 (30%), Positives = 29/53 (54%), Gaps = 1/53 (1%) Query: 313 IQLKHYDVTELDEKDEEMFSDEDDIKPEPSKYDVVATVPNTAELKSKEGKSEW 365 ++++ +L + D+E+F D + PE K A+ N E+KS +GK+ W Sbjct: 36 LEMRKGSELKLTKYDDEIFEDLINTFPE-FKDPKTASFVNEDEMKSAKGKAAW 87 >SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces pombe|chr 2|||Manual Length = 512 Score = 28.3 bits (60), Expect = 1.9 Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Query: 322 ELDEKDEEMFSDEDDIKPEPSKYDVVATVPNTAELKSKEGKS 363 E DE+ EE S+++D + E S+ DV AE +EG S Sbjct: 466 EGDEEMEEALSEDEDFQAE-SESDVAEEYDENAESSDEEGAS 506 >SPBC6B1.09c |nbs1||Mre11 complex subunit Nbs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 27.9 bits (59), Expect = 2.5 Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 2/50 (4%) Query: 324 DEKDEEMFSDED-DIKPEPSKYDVVATVPNTAELKSKEGKSEWIEVSSDS 372 + K E+F +D D++P P V ++P A K GKS + SS+S Sbjct: 542 EHKKTEVFDQDDTDLEPVPRLMSKVESIPAGAS-SDKSGKSSISKKSSNS 590 >SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 677 Score = 27.9 bits (59), Expect = 2.5 Identities = 14/51 (27%), Positives = 30/51 (58%), Gaps = 3/51 (5%) Query: 231 LVLGMNQYREVCLLDLTGAAIYTSNIVHKALLSAADKCKKYVEIIKNAVAK 281 L+ G+ ++ + +D+T Y +NI++K + +++CK Y+E+ A K Sbjct: 226 LISGIFKHLKSKTIDVTE---YYANIINKNAVLLSERCKAYIELSGKAQIK 273 >SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1328 Score = 27.9 bits (59), Expect = 2.5 Identities = 21/82 (25%), Positives = 41/82 (50%), Gaps = 13/82 (15%) Query: 292 INFSDIITTE--HMLALSRKDLNIQLKHYDVTELDEKDEEMFSDEDDIKPEPSKYDVVAT 349 ++FS II + H+L ++ +DL DE ++E+ ++D+I+ KYD + Sbjct: 429 LSFSKIIKVDGTHLLRITFRDLEFN---------DEDEDEI--EQDEIERVLQKYDNIPL 477 Query: 350 VPNTAELKSKEGKSEWIEVSSD 371 + LK K + ++I+ D Sbjct: 478 LNEEQALKEKNVEKDFIQWKDD 499 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 2.5 Identities = 23/99 (23%), Positives = 43/99 (43%), Gaps = 6/99 (6%) Query: 251 IYTSNIVHKALLSAADKCKKYVEIIKNAVAKDDN---LRQKREKINFSD-IITTEHMLAL 306 I T ++ H+A A+ + ++ V K+DN + +E IN D + T+ Sbjct: 12 IQTGSLYHRATFGLAESLEPPTIRVRTRVGKNDNGEYVFTNKEDINMEDPNVKTDETKVE 71 Query: 307 SRKDLNIQLKHYDVTELDEKDEEMFSD--EDDIKPEPSK 343 + + + Q + +VTE S+ DD P P++ Sbjct: 72 TSESTSEQPSNSNVTEEKNMGSSFHSECKVDDFTPLPNE 110 >SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 569 Score = 27.1 bits (57), Expect = 4.3 Identities = 21/89 (23%), Positives = 41/89 (46%), Gaps = 1/89 (1%) Query: 280 AKDDNLRQKREKINFSDIITTEHMLALSR-KDLNIQLKHYDVTELDEKDEEMFSDEDDIK 338 AK+ + + +K +D TE + + K+ +++ K D E+D+K+E+ S E K Sbjct: 460 AKETDSIEANDKDEKTDSKETEDKVEETESKEADVKAKETDSIEVDDKEEKTDSKETADK 519 Query: 339 PEPSKYDVVATVPNTAELKSKEGKSEWIE 367 E + P + K K+E ++ Sbjct: 520 VEQTDSKDTNEKPAKDDNKEANEKAEKVD 548 >SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1373 Score = 26.6 bits (56), Expect = 5.7 Identities = 12/49 (24%), Positives = 25/49 (51%) Query: 288 KREKINFSDIITTEHMLALSRKDLNIQLKHYDVTELDEKDEEMFSDEDD 336 K E +N+ DII E M + ++ + + E + ++EE +++D Sbjct: 936 KAEDLNWDDIIPEEEMERIEEEERMLAAQRAKEEERERREEEERENDED 984 >SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosaccharomyces pombe|chr 2|||Manual Length = 2335 Score = 26.6 bits (56), Expect = 5.7 Identities = 12/39 (30%), Positives = 23/39 (58%) Query: 102 LVRLLEKCYKDSKCIDLESLCIVVEEKVWSLRINIKVLN 140 LVR+L K D + + +++LC +V + + I I ++N Sbjct: 1014 LVRMLGKSNGDLRAVIMDTLCAIVSQLGYDYSIFIPMVN 1052 >SPAC26F1.09 |gyp51||GTPase activating protein Gyp51 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1031 Score = 26.6 bits (56), Expect = 5.7 Identities = 20/90 (22%), Positives = 44/90 (48%), Gaps = 5/90 (5%) Query: 270 KYVEIIKNAVAKDDNLRQKREKINFSDIITTEHMLALSRKDLNIQLKHYDVTELDEKDEE 329 +Y ++K+ D +L + R + + EH + LS N+Q++H +++ L K E+ Sbjct: 879 EYDILLKSEHELDSSLEEMRNRHKSLN----EHFIMLSDSMANLQVEHENMSALLLK-EK 933 Query: 330 MFSDEDDIKPEPSKYDVVATVPNTAELKSK 359 M+ ++ K ++ + A+ KS+ Sbjct: 934 MYLKNQTVEQASLKSEIASLNSQLAKQKSE 963 >SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 745 Score = 26.6 bits (56), Expect = 5.7 Identities = 23/83 (27%), Positives = 33/83 (39%), Gaps = 2/83 (2%) Query: 284 NLRQKREKINFSDIITTEHMLALSRKD--LNIQLKHYDVTELDEKDEEMFSDEDDIKPEP 341 N + N +D + H++A + NIQLK L E E SDE+ + Sbjct: 444 NRHISKRSSNSNDTAPSSHIIADKNLEPTSNIQLKKNPDGNLVETSELSDSDENSVLSNK 503 Query: 342 SKYDVVATVPNTAELKSKEGKSE 364 S V NT+ LK S+ Sbjct: 504 SSTLSKKVVENTSPLKYTHSASK 526 >SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 425 Score = 26.2 bits (55), Expect = 7.5 Identities = 23/96 (23%), Positives = 41/96 (42%), Gaps = 3/96 (3%) Query: 243 LLDLTGAAIYTSNIVHKALLSAADKCK--KYVEIIKNA-VAKDDNLRQKREKINFSDIIT 299 L D+T + T + +K L + + + EI+K + + D++R+K +I ++ Sbjct: 32 LKDITEEDVGTDDSCNKKFLEVQKQKEDEEDEEILKGVDLTQQDSVREKIHEIQSMSQLS 91 Query: 300 TEHMLALSRKDLNIQLKHYDVTELDEKDEEMFSDED 335 + L +K L Y T E DE S D Sbjct: 92 EKRKALLMQKMLMSGYLKYRRTHKKESDENQLSSSD 127 >SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 860 Score = 25.8 bits (54), Expect = 10.0 Identities = 13/45 (28%), Positives = 22/45 (48%), Gaps = 1/45 (2%) Query: 302 HMLALSRKDLNIQLKHYDVTELDEKDEEMFSD-EDDIKPEPSKYD 345 H L ++ L + ++ + D +EE+F D E+D E K D Sbjct: 497 HQLVIATTALRSMFTNAEIHDFDGDEEEVFKDVEEDDVSEDQKVD 541 >SPAC683.02c ||SPAC694.01c|zf-CCHC type zinc finger protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 218 Score = 25.8 bits (54), Expect = 10.0 Identities = 16/56 (28%), Positives = 25/56 (44%), Gaps = 1/56 (1%) Query: 16 KIVSQGHRLDGRNFNETRKLDISFGSEYGCCIVSLGETKILSEVTCEVAQPKQIRP 71 K S H L ++ ++ K D+SFG G + + + E VA P + RP Sbjct: 154 KFCSSVHHL-AKDCDQVNKDDVSFGHVVGVAGTTGADEDVYHEYAKTVAAPTKKRP 208 >SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 25.8 bits (54), Expect = 10.0 Identities = 18/93 (19%), Positives = 41/93 (44%), Gaps = 1/93 (1%) Query: 263 SAADKCKKYVEIIKNAVAKDDNLRQKREKINFSDIITTEHMLALSRKDLNIQLKHYDVTE 322 +A D + + + A D L Q D++ + ++++ ++ Y+ Sbjct: 325 NAVDNKILLIAMSSGSEALDAILAQMGGTEKVEDVLENVNDTLARSEEIDATIQTYNPQN 384 Query: 323 LDEKDEEMFSDEDDIKPEPSKY-DVVATVPNTA 354 +D +DE + + D+ E K D+V+T+ N + Sbjct: 385 IDLEDEAVEKEWQDLVAEEQKVEDIVSTLGNVS 417 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.317 0.134 0.388 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,779,655 Number of Sequences: 5004 Number of extensions: 76386 Number of successful extensions: 278 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 266 Number of HSP's gapped (non-prelim): 28 length of query: 375 length of database: 2,362,478 effective HSP length: 74 effective length of query: 301 effective length of database: 1,992,182 effective search space: 599646782 effective search space used: 599646782 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 54 (25.8 bits)
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