BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001130-TA|BGIBMGA001130-PA|IPR002524|Cation efflux protein (353 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_24101| Best HMM Match : No HMM Matches (HMM E-Value=.) 284 7e-77 SB_33717| Best HMM Match : No HMM Matches (HMM E-Value=.) 112 5e-25 SB_47415| Best HMM Match : No HMM Matches (HMM E-Value=.) 101 1e-21 SB_7396| Best HMM Match : DUF1634 (HMM E-Value=2.4) 56 4e-08 SB_21592| Best HMM Match : No HMM Matches (HMM E-Value=.) 48 2e-05 SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.) 43 4e-04 SB_53259| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 1.4 SB_34124| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.7 SB_42041| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.7 SB_42040| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.7 SB_3829| Best HMM Match : HLH (HMM E-Value=4.4e-12) 29 7.5 SB_24809| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 7.5 SB_20359| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 7.5 SB_3578| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 7.5 SB_1060| Best HMM Match : Tropomyosin (HMM E-Value=8.5) 28 10.0 SB_52575| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 10.0 SB_39435| Best HMM Match : 7tm_1 (HMM E-Value=4.60004e-41) 28 10.0 SB_2375| Best HMM Match : Pentapeptide_2 (HMM E-Value=2.7) 28 10.0 >SB_24101| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 519 Score = 284 bits (697), Expect = 7e-77 Identities = 170/373 (45%), Positives = 213/373 (57%), Gaps = 41/373 (10%) Query: 6 GKKCRLLCMLCLTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSPK-- 63 GK +L ML LT FF VELIVGY TNSMALVADSFHMLSDV +LII + +++ S K Sbjct: 13 GKTGSVLSMLALTSSFFLVELIVGYATNSMALVADSFHMLSDVMSLIIGYFALRYSKKSQ 72 Query: 64 KWSKNTFGWARAEVLGALVNAVFLVALCFSITVEAVKRFIEFEVIHNPKXXXXXXXXXX- 122 + +NTFGW RAEVLGALVNAVFL+ALCFSI VE++KR +E E IHN Sbjct: 73 RTERNTFGWQRAEVLGALVNAVFLIALCFSILVESLKRIVEVEPIHNVNLLLIVGAAGLF 132 Query: 123 ----------------XXXXXXXXXXHVPPPSNVRHLTEIVNSNADRALGHNSTDNEETD 166 H PP+ L ++ + ++ H+S + D Sbjct: 133 INLIGLLMFHRHGHGHSHGGHGHGHSHGLPPAAANGL--VILPGEENSINHSSGSLTD-D 189 Query: 167 EMVKKAKTQNHKSCKSSSDPG------HMNMKGVFLHVLSDALGSLIVVGSALVVWLTEW 220 K + SS G +NM+ V+LHVL DALGS+IV+ S ++ Sbjct: 190 HGAKNGHVHGGEEAVSSPLTGKQTSSAQLNMRAVYLHVLGDALGSVIVMVSGFIIKYASG 249 Query: 221 KYKHYIDPXXXXXXXXXXXXXXWPLLRESSLILLQTVPTHIQVDAIQRRLKN-VEGVMAV 279 K+ Y+DP PL+RESSLILLQTVPTHI++ +Q RL + V+GV++V Sbjct: 250 KWTLYVDPGMSILLVCLILRTSIPLMRESSLILLQTVPTHIKIKEVQDRLLDYVDGVLSV 309 Query: 280 HEFHVWQLAGDRII------------ASAHIRCRNLSEYMKIAEKVKEFFHNEGIHSTTI 327 HEFHVWQLAGD+II ASAHI C EYM IA ++K+FFHNEGIHSTTI Sbjct: 310 HEFHVWQLAGDKIIGLINTMLSLSLPASAHITCHTPDEYMAIANQIKQFFHNEGIHSTTI 369 Query: 328 QPEFVEGPQDENE 340 QPEFVE + E Sbjct: 370 QPEFVEDTLKKEE 382 >SB_33717| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 339 Score = 112 bits (269), Expect = 5e-25 Identities = 81/336 (24%), Positives = 150/336 (44%), Gaps = 18/336 (5%) Query: 7 KKCRLLCMLCLTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSPKKWS 66 ++ R+ LCL F E + GY +NS+A++ D+ HMLSD A +I+ ++ ++ + + Sbjct: 10 RRLRIASFLCLF--FMIAEFVGGYFSNSLAIMTDAAHMLSDFAGFMISLFAIWVATRPAT 67 Query: 67 KN-TFGWARAEVLGALVNAVFLVALCFSITVEAVKRFIEFEVIHNPKXXXXXXXXXXXXX 125 K ++GW RAEV+GA+++ + + L + A++R I E + K Sbjct: 68 KTLSYGWYRAEVMGAVLSVLVIWVLTGVLVYMALQRLIMKEYEVDAKVMLITASGGLLIN 127 Query: 126 XXXXXXXHVPPPSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSD 185 + + + GH+ T ++ K+ + S + + Sbjct: 128 VIMGAILYQKGHGH--------SHGGMSGHGHSHTTRGQSHSHTSPGKSPS--SVVTIEE 177 Query: 186 PGHMNMKGVFLHVLSDALGSLIVVGSALVVWLT-EWKYKHYIDPXXXXXXXXXXXXXXWP 244 ++N++ F+HV+ D L SL V +AL++W EW DP Sbjct: 178 EENVNVRAAFIHVIGDFLQSLGVFVAALIIWFKPEWAIA---DPICTFIFSIIVLFTTLA 234 Query: 245 LLRESSLILLQTVPTHIQVDAIQRRLKNVEGVMAVHEFHVWQLAGDRIIASAHIRCRNLS 304 +L+++ ++L++ +P + + ++ L + GV AVH+ HVW L S H+ N + Sbjct: 235 ILKDALIVLMEGMPKGLSFNDLKTSLTAIPGVEAVHDLHVWSLTVGTDALSVHLVVDNSA 294 Query: 305 EYMKIAEKVKEFFHNE-GIHSTTIQPEFVEGPQDEN 339 I E + IH +TIQ E G + E+ Sbjct: 295 NTQDILEAASQVCSKSFDIHHSTIQIETDNGSRKES 330 >SB_47415| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 584 Score = 101 bits (241), Expect = 1e-21 Identities = 74/300 (24%), Positives = 140/300 (46%), Gaps = 26/300 (8%) Query: 3 RYSGKKCRLLCMLCLTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSP 62 R + KK ++CL F E++ GY+ +S+A++ D+ HMLSD AA +I+ ++ ++ Sbjct: 140 RRTRKKLTTASVVCLF--FVVAEVVGGYLAHSLAIMTDAAHMLSDFAAFMISLFAIWVAE 197 Query: 63 -KKWSKNTFGWARAEVLGALVNAVFLVALCFSITVEAVKRFIEFEVIHNPKXXXXXXXXX 121 + K TFG+ RAE+LGALV+ + + L + AV+R I + N Sbjct: 198 WQPDKKRTFGYYRAEILGALVSVLIIWVLTGILVYLAVQRVITKDFDINADIMLITAGVA 257 Query: 122 XXXXXXXXXXXHVPPPSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCK 181 H S V H GH S D + + +H + Sbjct: 258 LGINILLGIILH---QSGVGH-------------GH-SHGGGGHDHGGSHSHSASHGKPR 300 Query: 182 SSS--DPGHMNMKGVFLHVLSDALGSL-IVVGSALVVWLTEWKYKHYIDPXXXXXXXXXX 238 S S + ++N++ F+HVL D + S+ +++ + ++ + WK DP Sbjct: 301 SGSVLEKENINVRAAFIHVLGDVVQSVGVLIAAYIIKYKPSWK---LADPICTFLFSILV 357 Query: 239 XXXXWPLLRESSLILLQTVPTHIQVDAIQRRLKNVEGVMAVHEFHVWQLAGDRIIASAHI 298 ++R++ +L++ P ++ +A++ L+N++GV+A H H+W L ++ + H+ Sbjct: 358 LITTLNIVRDTIHVLMEGTPKNVNYNAVKAGLENIQGVVAAHSLHIWSLTVNKAALAVHL 417 >SB_7396| Best HMM Match : DUF1634 (HMM E-Value=2.4) Length = 119 Score = 56.0 bits (129), Expect = 4e-08 Identities = 32/121 (26%), Positives = 61/121 (50%), Gaps = 5/121 (4%) Query: 194 VFLHVLSDALGSLIVVGSALVVWLTEWKYKHYIDPXXXXXXXXXXXXXXWPLLRESSLIL 253 VFLHV++D LGS+ V+ S++++ + DP PLL+ESSL+L Sbjct: 2 VFLHVVADTLGSVGVIASSILI---DQFGLFIADPVCSLFIASLIFLSVLPLLKESSLVL 58 Query: 254 LQTVPTHIQ--VDAIQRRLKNVEGVMAVHEFHVWQLAGDRIIASAHIRCRNLSEYMKIAE 311 LQ P ++ + ++ + +GV++ + H WQ + + + H++ + KI + Sbjct: 59 LQRTPLDMEKSLTGALNKILSFDGVLSFRDHHFWQHSESVVCGTIHVQVAPSASEQKIIQ 118 Query: 312 K 312 + Sbjct: 119 Q 119 >SB_21592| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 170 Score = 47.6 bits (108), Expect = 2e-05 Identities = 26/63 (41%), Positives = 37/63 (58%), Gaps = 1/63 (1%) Query: 10 RLLCMLCLTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSPKKWSKNT 69 ++ LCL F VEL+ G TNS+ L++D FHML D AL++ + MS K S+ T Sbjct: 89 QIFYFLCLNLMFTGVELLYGVWTNSLGLISDGFHMLFDCTALVLGLCASLMSRWKASR-T 147 Query: 70 FGW 72 F + Sbjct: 148 FSY 150 >SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 125 Score = 42.7 bits (96), Expect = 4e-04 Identities = 17/56 (30%), Positives = 33/56 (58%) Query: 243 WPLLRESSLILLQTVPTHIQVDAIQRRLKNVEGVMAVHEFHVWQLAGDRIIASAHI 298 + ++R+S IL + VP +++ ++R L + V++ H HVW L R + +AH+ Sbjct: 23 YAVIRDSFNILFEGVPRDVRIAEVKRDLSTLHHVVSFHNVHVWALTSGRNLLTAHL 78 >SB_53259| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 345 Score = 31.1 bits (67), Expect = 1.4 Identities = 22/65 (33%), Positives = 33/65 (50%), Gaps = 2/65 (3%) Query: 145 EIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFLHVLSDALG 204 E ++ ++D+ S DN ETD KK+K K S +P +KG FLH+ + + Sbjct: 60 ETLSRDSDKTEVSQSEDNTETDATNKKSKWMG-KIGFFSGNPSVETVKG-FLHIYKNKIC 117 Query: 205 SLIVV 209 SL V Sbjct: 118 SLTQV 122 >SB_34124| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 468 Score = 29.1 bits (62), Expect = 5.7 Identities = 10/26 (38%), Positives = 16/26 (61%) Query: 1 MGRYSGKKCRLLCMLCLTGGFFFVEL 26 +G Y G++C C+ C GGF+ E+ Sbjct: 237 VGTYMGEECATCCIKCPAGGFYQDEI 262 >SB_42041| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 478 Score = 29.1 bits (62), Expect = 5.7 Identities = 18/73 (24%), Positives = 34/73 (46%), Gaps = 1/73 (1%) Query: 133 HVPPPSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMK 192 H+ P ++ + + +N+ D L N E D + K ++K ++ PGH+N K Sbjct: 291 HLNKPGHLNNKPDHLNNKPDH-LNEPDHLNNEPDHLNNKPDHLDNKPYHLNNKPGHLNNK 349 Query: 193 GVFLHVLSDALGS 205 L+ +D L + Sbjct: 350 PDHLNNKTDHLNN 362 >SB_42040| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 320 Score = 29.1 bits (62), Expect = 5.7 Identities = 17/69 (24%), Positives = 31/69 (44%) Query: 137 PSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFL 196 P N+ ++E +N+ D + N + D + K N+K ++ P H+N K L Sbjct: 194 PDNLNKMSEHLNNKPDHLNKMSEHLNNKPDHLDNKPYHLNNKPDHRNNKPDHLNNKPEHL 253 Query: 197 HVLSDALGS 205 + D L + Sbjct: 254 NNKPDHLNN 262 Score = 28.3 bits (60), Expect = 10.0 Identities = 15/69 (21%), Positives = 32/69 (46%) Query: 137 PSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFL 196 P ++ + + +N+ +D N E D + K + N+K ++P H+N K L Sbjct: 110 PDHLNNKPDHLNNKSDHLNNKPYHLNNEPDHLYNKPEHLNNKPNHLDNEPDHLNNKPDHL 169 Query: 197 HVLSDALGS 205 + + + L + Sbjct: 170 NKMPEHLNN 178 >SB_3829| Best HMM Match : HLH (HMM E-Value=4.4e-12) Length = 1650 Score = 28.7 bits (61), Expect = 7.5 Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Query: 138 SNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAK-TQNHKSCKSS 183 SN + L + SN D + G T N+ D + ++K T ++K +SS Sbjct: 715 SNQQSLGQAQTSNEDNSRGSEGTSNDHMDGLFSQSKPTMDNKQTQSS 761 >SB_24809| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 780 Score = 28.7 bits (61), Expect = 7.5 Identities = 18/72 (25%), Positives = 32/72 (44%), Gaps = 5/72 (6%) Query: 137 PSNVRHLTE-----IVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNM 191 P NVR L E I++ N + D+ + ++ + + S+ DP H + Sbjct: 344 PLNVRRLQEQDGSHIIDMNFLESQLQAKEDSPASTSLLSSVHSPTTSTPSSAMDPFHGKI 403 Query: 192 KGVFLHVLSDAL 203 G+F H+ +D L Sbjct: 404 YGIFEHITTDKL 415 >SB_20359| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 4700 Score = 28.7 bits (61), Expect = 7.5 Identities = 15/47 (31%), Positives = 22/47 (46%), Gaps = 1/47 (2%) Query: 161 DNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFLHVLSDALGSLI 207 D T+ +K K QNH + + P H + + L D LGSL+ Sbjct: 1087 DCASTESALKVGKCQNHYQLEQYNPPRHCQW-AIIVPTLLDCLGSLL 1132 >SB_3578| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 355 Score = 28.7 bits (61), Expect = 7.5 Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 1/51 (1%) Query: 136 PPSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKK-AKTQNHKSCKSSSD 185 P +R L + + R+ G S D+E + VKK K +N+ K SSD Sbjct: 94 PYRELRDLETVSEERSKRSSGDKSDDSEIASKEVKKDKKKKNYSKEKGSSD 144 >SB_1060| Best HMM Match : Tropomyosin (HMM E-Value=8.5) Length = 430 Score = 28.3 bits (60), Expect = 10.0 Identities = 17/67 (25%), Positives = 30/67 (44%) Query: 137 PSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFL 196 P N+ ++E +N+ D + N + D + K N+K ++ P H+N K L Sbjct: 352 PGNLNKMSEHLNNKPDHLNKMSEHLNNKPDHLDNKPYHLNNKPDHRNNKPDHLNNKPDHL 411 Query: 197 HVLSDAL 203 + D L Sbjct: 412 NNKPDHL 418 >SB_52575| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1267 Score = 28.3 bits (60), Expect = 10.0 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 2/48 (4%) Query: 133 HVPPPSNVRHLTEIVNS-NADRALGHNSTDNEETDEMVKKAKTQNHKS 179 H P PS++ + TEI + DR++ N E++E+ KK K + ++ Sbjct: 700 HSPDPSDLNNNTEIKREPSPDRSIDSPEESNNESEEL-KKRKQRRQRT 746 >SB_39435| Best HMM Match : 7tm_1 (HMM E-Value=4.60004e-41) Length = 352 Score = 28.3 bits (60), Expect = 10.0 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 4/75 (5%) Query: 30 YITNSMALVADSFHMLSDVAALIIAFLSV--KMSPKKWSKNTFGWARAEVLGALVNAVFL 87 Y+ ++AL AD +L D L F V W++ T W ++GA + VF+ Sbjct: 44 YLLVNLAL-ADVITLLMD-PFLHTTFFEVVANFGTNAWTQTTKDWLCRVLIGAGLLGVFV 101 Query: 88 VALCFSITVEAVKRF 102 + +T+ AV+R+ Sbjct: 102 LVSVLLLTIVAVERY 116 >SB_2375| Best HMM Match : Pentapeptide_2 (HMM E-Value=2.7) Length = 521 Score = 28.3 bits (60), Expect = 10.0 Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 2/44 (4%) Query: 148 NSNADRALGHNST-DNEETDEMVKKAKTQNHKSCKSSSDPGHMN 190 NSN+D + NST DN +D T ++ S K +S+P + N Sbjct: 212 NSNSDNSTSDNSTSDNSNSDNSYTDNSTSDN-SKKDNSNPNNSN 254 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.322 0.134 0.408 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,058,166 Number of Sequences: 59808 Number of extensions: 329379 Number of successful extensions: 952 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 871 Number of HSP's gapped (non-prelim): 84 length of query: 353 length of database: 16,821,457 effective HSP length: 83 effective length of query: 270 effective length of database: 11,857,393 effective search space: 3201496110 effective search space used: 3201496110 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 60 (28.3 bits)
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