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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001130-TA|BGIBMGA001130-PA|IPR002524|Cation efflux
protein
         (353 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_24101| Best HMM Match : No HMM Matches (HMM E-Value=.)             284   7e-77
SB_33717| Best HMM Match : No HMM Matches (HMM E-Value=.)             112   5e-25
SB_47415| Best HMM Match : No HMM Matches (HMM E-Value=.)             101   1e-21
SB_7396| Best HMM Match : DUF1634 (HMM E-Value=2.4)                    56   4e-08
SB_21592| Best HMM Match : No HMM Matches (HMM E-Value=.)              48   2e-05
SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)              43   4e-04
SB_53259| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   1.4  
SB_34124| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.7  
SB_42041| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.7  
SB_42040| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.7  
SB_3829| Best HMM Match : HLH (HMM E-Value=4.4e-12)                    29   7.5  
SB_24809| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   7.5  
SB_20359| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   7.5  
SB_3578| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   7.5  
SB_1060| Best HMM Match : Tropomyosin (HMM E-Value=8.5)                28   10.0 
SB_52575| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   10.0 
SB_39435| Best HMM Match : 7tm_1 (HMM E-Value=4.60004e-41)             28   10.0 
SB_2375| Best HMM Match : Pentapeptide_2 (HMM E-Value=2.7)             28   10.0 

>SB_24101| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 519

 Score =  284 bits (697), Expect = 7e-77
 Identities = 170/373 (45%), Positives = 213/373 (57%), Gaps = 41/373 (10%)

Query: 6   GKKCRLLCMLCLTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSPK-- 63
           GK   +L ML LT  FF VELIVGY TNSMALVADSFHMLSDV +LII + +++ S K  
Sbjct: 13  GKTGSVLSMLALTSSFFLVELIVGYATNSMALVADSFHMLSDVMSLIIGYFALRYSKKSQ 72

Query: 64  KWSKNTFGWARAEVLGALVNAVFLVALCFSITVEAVKRFIEFEVIHNPKXXXXXXXXXX- 122
           +  +NTFGW RAEVLGALVNAVFL+ALCFSI VE++KR +E E IHN             
Sbjct: 73  RTERNTFGWQRAEVLGALVNAVFLIALCFSILVESLKRIVEVEPIHNVNLLLIVGAAGLF 132

Query: 123 ----------------XXXXXXXXXXHVPPPSNVRHLTEIVNSNADRALGHNSTDNEETD 166
                                     H  PP+    L  ++    + ++ H+S    + D
Sbjct: 133 INLIGLLMFHRHGHGHSHGGHGHGHSHGLPPAAANGL--VILPGEENSINHSSGSLTD-D 189

Query: 167 EMVKKAKTQNHKSCKSSSDPG------HMNMKGVFLHVLSDALGSLIVVGSALVVWLTEW 220
              K       +   SS   G       +NM+ V+LHVL DALGS+IV+ S  ++     
Sbjct: 190 HGAKNGHVHGGEEAVSSPLTGKQTSSAQLNMRAVYLHVLGDALGSVIVMVSGFIIKYASG 249

Query: 221 KYKHYIDPXXXXXXXXXXXXXXWPLLRESSLILLQTVPTHIQVDAIQRRLKN-VEGVMAV 279
           K+  Y+DP               PL+RESSLILLQTVPTHI++  +Q RL + V+GV++V
Sbjct: 250 KWTLYVDPGMSILLVCLILRTSIPLMRESSLILLQTVPTHIKIKEVQDRLLDYVDGVLSV 309

Query: 280 HEFHVWQLAGDRII------------ASAHIRCRNLSEYMKIAEKVKEFFHNEGIHSTTI 327
           HEFHVWQLAGD+II            ASAHI C    EYM IA ++K+FFHNEGIHSTTI
Sbjct: 310 HEFHVWQLAGDKIIGLINTMLSLSLPASAHITCHTPDEYMAIANQIKQFFHNEGIHSTTI 369

Query: 328 QPEFVEGPQDENE 340
           QPEFVE    + E
Sbjct: 370 QPEFVEDTLKKEE 382


>SB_33717| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 339

 Score =  112 bits (269), Expect = 5e-25
 Identities = 81/336 (24%), Positives = 150/336 (44%), Gaps = 18/336 (5%)

Query: 7   KKCRLLCMLCLTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSPKKWS 66
           ++ R+   LCL   F   E + GY +NS+A++ D+ HMLSD A  +I+  ++ ++ +  +
Sbjct: 10  RRLRIASFLCLF--FMIAEFVGGYFSNSLAIMTDAAHMLSDFAGFMISLFAIWVATRPAT 67

Query: 67  KN-TFGWARAEVLGALVNAVFLVALCFSITVEAVKRFIEFEVIHNPKXXXXXXXXXXXXX 125
           K  ++GW RAEV+GA+++ + +  L   +   A++R I  E   + K             
Sbjct: 68  KTLSYGWYRAEVMGAVLSVLVIWVLTGVLVYMALQRLIMKEYEVDAKVMLITASGGLLIN 127

Query: 126 XXXXXXXHVPPPSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSD 185
                  +     +        +       GH+ T   ++       K+ +  S  +  +
Sbjct: 128 VIMGAILYQKGHGH--------SHGGMSGHGHSHTTRGQSHSHTSPGKSPS--SVVTIEE 177

Query: 186 PGHMNMKGVFLHVLSDALGSLIVVGSALVVWLT-EWKYKHYIDPXXXXXXXXXXXXXXWP 244
             ++N++  F+HV+ D L SL V  +AL++W   EW      DP                
Sbjct: 178 EENVNVRAAFIHVIGDFLQSLGVFVAALIIWFKPEWAIA---DPICTFIFSIIVLFTTLA 234

Query: 245 LLRESSLILLQTVPTHIQVDAIQRRLKNVEGVMAVHEFHVWQLAGDRIIASAHIRCRNLS 304
           +L+++ ++L++ +P  +  + ++  L  + GV AVH+ HVW L       S H+   N +
Sbjct: 235 ILKDALIVLMEGMPKGLSFNDLKTSLTAIPGVEAVHDLHVWSLTVGTDALSVHLVVDNSA 294

Query: 305 EYMKIAEKVKEFFHNE-GIHSTTIQPEFVEGPQDEN 339
               I E   +       IH +TIQ E   G + E+
Sbjct: 295 NTQDILEAASQVCSKSFDIHHSTIQIETDNGSRKES 330


>SB_47415| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 584

 Score =  101 bits (241), Expect = 1e-21
 Identities = 74/300 (24%), Positives = 140/300 (46%), Gaps = 26/300 (8%)

Query: 3   RYSGKKCRLLCMLCLTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSP 62
           R + KK     ++CL   F   E++ GY+ +S+A++ D+ HMLSD AA +I+  ++ ++ 
Sbjct: 140 RRTRKKLTTASVVCLF--FVVAEVVGGYLAHSLAIMTDAAHMLSDFAAFMISLFAIWVAE 197

Query: 63  -KKWSKNTFGWARAEVLGALVNAVFLVALCFSITVEAVKRFIEFEVIHNPKXXXXXXXXX 121
            +   K TFG+ RAE+LGALV+ + +  L   +   AV+R I  +   N           
Sbjct: 198 WQPDKKRTFGYYRAEILGALVSVLIIWVLTGILVYLAVQRVITKDFDINADIMLITAGVA 257

Query: 122 XXXXXXXXXXXHVPPPSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCK 181
                      H    S V H             GH S      D     + + +H   +
Sbjct: 258 LGINILLGIILH---QSGVGH-------------GH-SHGGGGHDHGGSHSHSASHGKPR 300

Query: 182 SSS--DPGHMNMKGVFLHVLSDALGSL-IVVGSALVVWLTEWKYKHYIDPXXXXXXXXXX 238
           S S  +  ++N++  F+HVL D + S+ +++ + ++ +   WK     DP          
Sbjct: 301 SGSVLEKENINVRAAFIHVLGDVVQSVGVLIAAYIIKYKPSWK---LADPICTFLFSILV 357

Query: 239 XXXXWPLLRESSLILLQTVPTHIQVDAIQRRLKNVEGVMAVHEFHVWQLAGDRIIASAHI 298
                 ++R++  +L++  P ++  +A++  L+N++GV+A H  H+W L  ++   + H+
Sbjct: 358 LITTLNIVRDTIHVLMEGTPKNVNYNAVKAGLENIQGVVAAHSLHIWSLTVNKAALAVHL 417


>SB_7396| Best HMM Match : DUF1634 (HMM E-Value=2.4)
          Length = 119

 Score = 56.0 bits (129), Expect = 4e-08
 Identities = 32/121 (26%), Positives = 61/121 (50%), Gaps = 5/121 (4%)

Query: 194 VFLHVLSDALGSLIVVGSALVVWLTEWKYKHYIDPXXXXXXXXXXXXXXWPLLRESSLIL 253
           VFLHV++D LGS+ V+ S++++   +       DP               PLL+ESSL+L
Sbjct: 2   VFLHVVADTLGSVGVIASSILI---DQFGLFIADPVCSLFIASLIFLSVLPLLKESSLVL 58

Query: 254 LQTVPTHIQ--VDAIQRRLKNVEGVMAVHEFHVWQLAGDRIIASAHIRCRNLSEYMKIAE 311
           LQ  P  ++  +     ++ + +GV++  + H WQ +   +  + H++    +   KI +
Sbjct: 59  LQRTPLDMEKSLTGALNKILSFDGVLSFRDHHFWQHSESVVCGTIHVQVAPSASEQKIIQ 118

Query: 312 K 312
           +
Sbjct: 119 Q 119


>SB_21592| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 170

 Score = 47.6 bits (108), Expect = 2e-05
 Identities = 26/63 (41%), Positives = 37/63 (58%), Gaps = 1/63 (1%)

Query: 10  RLLCMLCLTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSPKKWSKNT 69
           ++   LCL   F  VEL+ G  TNS+ L++D FHML D  AL++   +  MS  K S+ T
Sbjct: 89  QIFYFLCLNLMFTGVELLYGVWTNSLGLISDGFHMLFDCTALVLGLCASLMSRWKASR-T 147

Query: 70  FGW 72
           F +
Sbjct: 148 FSY 150


>SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 125

 Score = 42.7 bits (96), Expect = 4e-04
 Identities = 17/56 (30%), Positives = 33/56 (58%)

Query: 243 WPLLRESSLILLQTVPTHIQVDAIQRRLKNVEGVMAVHEFHVWQLAGDRIIASAHI 298
           + ++R+S  IL + VP  +++  ++R L  +  V++ H  HVW L   R + +AH+
Sbjct: 23  YAVIRDSFNILFEGVPRDVRIAEVKRDLSTLHHVVSFHNVHVWALTSGRNLLTAHL 78


>SB_53259| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 345

 Score = 31.1 bits (67), Expect = 1.4
 Identities = 22/65 (33%), Positives = 33/65 (50%), Gaps = 2/65 (3%)

Query: 145 EIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFLHVLSDALG 204
           E ++ ++D+     S DN ETD   KK+K    K    S +P    +KG FLH+  + + 
Sbjct: 60  ETLSRDSDKTEVSQSEDNTETDATNKKSKWMG-KIGFFSGNPSVETVKG-FLHIYKNKIC 117

Query: 205 SLIVV 209
           SL  V
Sbjct: 118 SLTQV 122


>SB_34124| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 468

 Score = 29.1 bits (62), Expect = 5.7
 Identities = 10/26 (38%), Positives = 16/26 (61%)

Query: 1   MGRYSGKKCRLLCMLCLTGGFFFVEL 26
           +G Y G++C   C+ C  GGF+  E+
Sbjct: 237 VGTYMGEECATCCIKCPAGGFYQDEI 262


>SB_42041| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 478

 Score = 29.1 bits (62), Expect = 5.7
 Identities = 18/73 (24%), Positives = 34/73 (46%), Gaps = 1/73 (1%)

Query: 133 HVPPPSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMK 192
           H+  P ++ +  + +N+  D  L      N E D +  K    ++K    ++ PGH+N K
Sbjct: 291 HLNKPGHLNNKPDHLNNKPDH-LNEPDHLNNEPDHLNNKPDHLDNKPYHLNNKPGHLNNK 349

Query: 193 GVFLHVLSDALGS 205
              L+  +D L +
Sbjct: 350 PDHLNNKTDHLNN 362


>SB_42040| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 320

 Score = 29.1 bits (62), Expect = 5.7
 Identities = 17/69 (24%), Positives = 31/69 (44%)

Query: 137 PSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFL 196
           P N+  ++E +N+  D     +   N + D +  K    N+K    ++ P H+N K   L
Sbjct: 194 PDNLNKMSEHLNNKPDHLNKMSEHLNNKPDHLDNKPYHLNNKPDHRNNKPDHLNNKPEHL 253

Query: 197 HVLSDALGS 205
           +   D L +
Sbjct: 254 NNKPDHLNN 262



 Score = 28.3 bits (60), Expect = 10.0
 Identities = 15/69 (21%), Positives = 32/69 (46%)

Query: 137 PSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFL 196
           P ++ +  + +N+ +D         N E D +  K +  N+K     ++P H+N K   L
Sbjct: 110 PDHLNNKPDHLNNKSDHLNNKPYHLNNEPDHLYNKPEHLNNKPNHLDNEPDHLNNKPDHL 169

Query: 197 HVLSDALGS 205
           + + + L +
Sbjct: 170 NKMPEHLNN 178


>SB_3829| Best HMM Match : HLH (HMM E-Value=4.4e-12)
          Length = 1650

 Score = 28.7 bits (61), Expect = 7.5
 Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%)

Query: 138 SNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAK-TQNHKSCKSS 183
           SN + L +   SN D + G   T N+  D +  ++K T ++K  +SS
Sbjct: 715 SNQQSLGQAQTSNEDNSRGSEGTSNDHMDGLFSQSKPTMDNKQTQSS 761


>SB_24809| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 780

 Score = 28.7 bits (61), Expect = 7.5
 Identities = 18/72 (25%), Positives = 32/72 (44%), Gaps = 5/72 (6%)

Query: 137 PSNVRHLTE-----IVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNM 191
           P NVR L E     I++ N   +      D+  +  ++    +    +  S+ DP H  +
Sbjct: 344 PLNVRRLQEQDGSHIIDMNFLESQLQAKEDSPASTSLLSSVHSPTTSTPSSAMDPFHGKI 403

Query: 192 KGVFLHVLSDAL 203
            G+F H+ +D L
Sbjct: 404 YGIFEHITTDKL 415


>SB_20359| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 4700

 Score = 28.7 bits (61), Expect = 7.5
 Identities = 15/47 (31%), Positives = 22/47 (46%), Gaps = 1/47 (2%)

Query: 161  DNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFLHVLSDALGSLI 207
            D   T+  +K  K QNH   +  + P H     + +  L D LGSL+
Sbjct: 1087 DCASTESALKVGKCQNHYQLEQYNPPRHCQW-AIIVPTLLDCLGSLL 1132


>SB_3578| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 355

 Score = 28.7 bits (61), Expect = 7.5
 Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 1/51 (1%)

Query: 136 PPSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKK-AKTQNHKSCKSSSD 185
           P   +R L  +    + R+ G  S D+E   + VKK  K +N+   K SSD
Sbjct: 94  PYRELRDLETVSEERSKRSSGDKSDDSEIASKEVKKDKKKKNYSKEKGSSD 144


>SB_1060| Best HMM Match : Tropomyosin (HMM E-Value=8.5)
          Length = 430

 Score = 28.3 bits (60), Expect = 10.0
 Identities = 17/67 (25%), Positives = 30/67 (44%)

Query: 137 PSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFL 196
           P N+  ++E +N+  D     +   N + D +  K    N+K    ++ P H+N K   L
Sbjct: 352 PGNLNKMSEHLNNKPDHLNKMSEHLNNKPDHLDNKPYHLNNKPDHRNNKPDHLNNKPDHL 411

Query: 197 HVLSDAL 203
           +   D L
Sbjct: 412 NNKPDHL 418


>SB_52575| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1267

 Score = 28.3 bits (60), Expect = 10.0
 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 2/48 (4%)

Query: 133 HVPPPSNVRHLTEIVNS-NADRALGHNSTDNEETDEMVKKAKTQNHKS 179
           H P PS++ + TEI    + DR++      N E++E+ KK K +  ++
Sbjct: 700 HSPDPSDLNNNTEIKREPSPDRSIDSPEESNNESEEL-KKRKQRRQRT 746


>SB_39435| Best HMM Match : 7tm_1 (HMM E-Value=4.60004e-41)
          Length = 352

 Score = 28.3 bits (60), Expect = 10.0
 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 4/75 (5%)

Query: 30  YITNSMALVADSFHMLSDVAALIIAFLSV--KMSPKKWSKNTFGWARAEVLGALVNAVFL 87
           Y+  ++AL AD   +L D   L   F  V        W++ T  W    ++GA +  VF+
Sbjct: 44  YLLVNLAL-ADVITLLMD-PFLHTTFFEVVANFGTNAWTQTTKDWLCRVLIGAGLLGVFV 101

Query: 88  VALCFSITVEAVKRF 102
           +     +T+ AV+R+
Sbjct: 102 LVSVLLLTIVAVERY 116


>SB_2375| Best HMM Match : Pentapeptide_2 (HMM E-Value=2.7)
          Length = 521

 Score = 28.3 bits (60), Expect = 10.0
 Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 2/44 (4%)

Query: 148 NSNADRALGHNST-DNEETDEMVKKAKTQNHKSCKSSSDPGHMN 190
           NSN+D +   NST DN  +D       T ++ S K +S+P + N
Sbjct: 212 NSNSDNSTSDNSTSDNSNSDNSYTDNSTSDN-SKKDNSNPNNSN 254


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.322    0.134    0.408 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,058,166
Number of Sequences: 59808
Number of extensions: 329379
Number of successful extensions: 952
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 871
Number of HSP's gapped (non-prelim): 84
length of query: 353
length of database: 16,821,457
effective HSP length: 83
effective length of query: 270
effective length of database: 11,857,393
effective search space: 3201496110
effective search space used: 3201496110
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 60 (28.3 bits)

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