Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001130-TA|BGIBMGA001130-PA|IPR002524|Cation efflux
protein
(353 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23C11.14 |zhf1|zhf, zhf|zinc ion transporter Zhf1|Schizosacc... 112 6e-26
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 60 6e-10
SPCC1020.03 |||mitochondrial iron ion transporter|Schizosaccharo... 35 0.015
SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual 30 0.57
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 28 1.7
SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|... 28 2.3
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 28 2.3
SPBC29A3.15c |rsm23||mitochondrial ribosomal protein subunit S23... 27 3.0
SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2||... 27 5.3
SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pom... 26 7.0
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 26 7.0
>SPAC23C11.14 |zhf1|zhf, zhf|zinc ion transporter
Zhf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 387
Score = 112 bits (270), Expect = 6e-26
Identities = 54/153 (35%), Positives = 88/153 (57%), Gaps = 6/153 (3%)
Query: 188 HMNMKGVFLHVLSDALGSLIVVGSALVVWLTEWKYKHYIDPXXXXXXXXXXXXXXWPLLR 247
++NM GVFLHVL DALG++ V+ +AL + T++ ++ DP PL +
Sbjct: 227 NLNMHGVFLHVLGDALGNIGVISAALFIKYTDYSWRFLFDPCISILLTFIILFSAIPLCK 286
Query: 248 ESSLILLQTVPTHIQVDAIQRRLKNVEGVMAVHEFHVWQLAGDRIIASAHIRCRNLSE-- 305
++LILLQ P I++D + + +++GV +VHE H+WQL+ ++IA+ H+ C L +
Sbjct: 287 SAALILLQVAPQSIKLDDVSNLINHLDGVESVHELHIWQLSDVKLIATVHV-CVTLPDDK 345
Query: 306 ---YMKIAEKVKEFFHNEGIHSTTIQPEFVEGP 335
Y K+ ++ + GI+ TIQPEF P
Sbjct: 346 GESYTKLTTDIRNVLQSFGIYDVTIQPEFANHP 378
Score = 109 bits (261), Expect = 8e-25
Identities = 50/107 (46%), Positives = 76/107 (71%), Gaps = 2/107 (1%)
Query: 7 KKCRLLCMLCLTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKM--SPKK 64
++ R++ +L + FFF+E+I GY +S+AL+ADSFHML+D+ +L++A + ++ S
Sbjct: 6 RQTRIILLLGIDVTFFFIEIITGYAIDSLALIADSFHMLNDIVSLLVALWATRLAHSTSH 65
Query: 65 WSKNTFGWARAEVLGALVNAVFLVALCFSITVEAVKRFIEFEVIHNP 111
K T+GW RAE+LGAL N VFL+ALC I +EA++RFIE + NP
Sbjct: 66 EPKYTYGWQRAEILGALSNGVFLIALCMFIFMEAIERFIEPPSVSNP 112
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 59.7 bits (138), Expect = 6e-10
Identities = 29/86 (33%), Positives = 49/86 (56%), Gaps = 1/86 (1%)
Query: 17 LTGGFFFVELIVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSPKKWS-KNTFGWARA 75
L + FV++I G TNS+ L++D+ HM D A+++ ++ ++ + FG+A+
Sbjct: 356 LNVSYMFVQVIYGLWTNSLGLISDAIHMAFDCIAILVGLVATTLAKMPLNYAYPFGFAKI 415
Query: 76 EVLGALVNAVFLVALCFSITVEAVKR 101
E L N +FLV + FSI EA+ R
Sbjct: 416 EALSGFTNGIFLVLISFSIVGEALYR 441
Score = 40.7 bits (91), Expect = 3e-04
Identities = 26/95 (27%), Positives = 45/95 (47%), Gaps = 8/95 (8%)
Query: 162 NEETD-EMVKKAKTQNHKSCKSSSDPGHMNMKGVFLHVLSDALGSL-IVVGSALVVWLTE 219
NE+ D E + N C + + NM G+FLH+++D +GS+ ++V + L+ W +
Sbjct: 555 NEKCDHESISLQNLDNDHHCHHHHE--NHNMHGIFLHIIADTMGSVGVIVSTILIQWFS- 611
Query: 220 WKYKHYIDPXXXXXXXXXXXXXXWPLLRESSLILL 254
W DP PL+++S+ LL
Sbjct: 612 WT---GFDPLASLIIAALIFVSVLPLIKDSAKNLL 643
>SPCC1020.03 |||mitochondrial iron ion
transporter|Schizosaccharomyces pombe|chr 3|||Manual
Length = 397
Score = 35.1 bits (77), Expect = 0.015
Identities = 22/76 (28%), Positives = 36/76 (47%), Gaps = 1/76 (1%)
Query: 27 IVGYITNSMALVADSFHMLSDVAALIIAFLSVKMSPKK-WSKNTFGWARAEVLGALVNAV 85
I G S LVAD+ H L D + ++ ++K+ KK K G+ + E +G +
Sbjct: 93 IGGVALQSSILVADAAHQLGDTLSDLVTLATLKICSKKPTQKYPAGFGKWETIGTFTVSG 152
Query: 86 FLVALCFSITVEAVKR 101
LVA+ I ++ R
Sbjct: 153 LLVAVSVGIAHSSLSR 168
>SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 684
Score = 29.9 bits (64), Expect = 0.57
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
Query: 161 DNEETDEMVKKAKTQNHKSCKSSSD---PGHMNMKGVFLH 197
+++E ++ V K++N++S K SS PG M +G F H
Sbjct: 162 NSDEDEDSVSNDKSKNNESLKKSSKNEIPGFMRQRGRFFH 201
>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 836
Score = 28.3 bits (60), Expect = 1.7
Identities = 14/39 (35%), Positives = 18/39 (46%)
Query: 150 NADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSDPGH 188
N R LG NS ++T M + K N + SS GH
Sbjct: 242 NGPRTLGKNSKRADDTARMASRMKPSNFNNNIQSSSYGH 280
>SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|chr
3|||Manual
Length = 700
Score = 27.9 bits (59), Expect = 2.3
Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 3/27 (11%)
Query: 308 KIAEKVKEFFHNEGIH---STTIQPEF 331
++AEKVK FFH GI+ TT+ P +
Sbjct: 632 QVAEKVKRFFHYYGINRHKMTTLTPSY 658
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 27.9 bits (59), Expect = 2.3
Identities = 12/38 (31%), Positives = 26/38 (68%)
Query: 148 NSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSD 185
+S+++ +S+++EE +E+V+K + + S +SSSD
Sbjct: 170 SSSSESESESSSSESEEEEEVVEKTEEKKEGSSESSSD 207
>SPBC29A3.15c |rsm23||mitochondrial ribosomal protein subunit
S23|Schizosaccharomyces pombe|chr 2|||Manual
Length = 476
Score = 27.5 bits (58), Expect = 3.0
Identities = 18/51 (35%), Positives = 24/51 (47%), Gaps = 2/51 (3%)
Query: 135 PPPSNVRHLTEIVNSNADRALGHNSTDNEETDEMVKKAKTQNHKSCKSSSD 185
P S+V T I NS+ L H + E T E VK ++ H S S S+
Sbjct: 87 PSTSSVEKSTTIPNSSV--VLDHLLNEGENTLEEVKPSEIHPHMSWSSKSE 135
>SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr
2|||Manual
Length = 525
Score = 26.6 bits (56), Expect = 5.3
Identities = 23/63 (36%), Positives = 33/63 (52%), Gaps = 5/63 (7%)
Query: 165 TDEMVKK-AKTQNHKSCKSSSDPGHMNMKGVFLHVLSDALGSLIVVGSALVVWLTEWKYK 223
TD + KK AK QN S P H + F+H LS + +L+ + SA+ WL +Y
Sbjct: 293 TDSIDKKIAKLQN--STNIFYVPEHEPILA-FVHQLSSSYTNLLNLRSAICDWLKFSRY- 348
Query: 224 HYI 226
HY+
Sbjct: 349 HYL 351
>SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 450
Score = 26.2 bits (55), Expect = 7.0
Identities = 13/45 (28%), Positives = 22/45 (48%)
Query: 158 NSTDNEETDEMVKKAKTQNHKSCKSSSDPGHMNMKGVFLHVLSDA 202
NS N+E + + K +N + +P H++ + V H L DA
Sbjct: 30 NSLPNKEIEGKNEVIKNKNKLDNGNEIEPQHLSQELVLQHALQDA 74
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 7.0
Identities = 16/43 (37%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Query: 291 RIIASAHIRCRNLSEYMKIAEKVKEFFHNEGIHSTTIQPEFVE 333
R I ++ NL +K+A KVK H +H Q EFVE
Sbjct: 162 RHIGLSNFNDTNLERILKVA-KVKPAVHQMELHPFLPQTEFVE 203
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.322 0.134 0.408
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,399,896
Number of Sequences: 5004
Number of extensions: 49401
Number of successful extensions: 160
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 146
Number of HSP's gapped (non-prelim): 14
length of query: 353
length of database: 2,362,478
effective HSP length: 73
effective length of query: 280
effective length of database: 1,997,186
effective search space: 559212080
effective search space used: 559212080
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 54 (25.8 bits)
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