Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001126-TA|BGIBMGA001126-PA|IPR002005|Rab GTPase
activator, IPR000632|Yeast Mrs6p protein
         (523 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein |Sch...   127   3e-30
SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schiz...   101   2e-22
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c...    31   0.52 
SPBC115.02c |||AFG1 family mitochondrial ATPase|Schizosaccharomy...    29   1.6  
SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra...    29   1.6  
SPAC9E9.12c |ybt1|abc1|ABC transporter Ybt1|Schizosaccharomyces ...    29   1.6  
SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica...    28   3.6  
SPBC83.03c |tas3||RITS complex subunit 3 |Schizosaccharomyces po...    27   6.4  
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po...    27   6.4  
SPBC336.02 |||18S rRNA dimethylase|Schizosaccharomyces pombe|chr...    27   8.4  
SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po...    27   8.4  

>SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 459

 Score =  127 bits (307), Expect = 3e-30
 Identities = 108/428 (25%), Positives = 188/428 (43%), Gaps = 30/428 (7%)

Query: 5   DFPTDFQVIVVGTGMVESIVAAACSRIGKNVLHMDSSDHYGGLWASYNFEGLQKFIKEVN 64
           D P  + VI+VGT +  SI++AA S   + VLH+D +  YG +  S     L++  +++ 
Sbjct: 2   DDPNSYDVIIVGTNLRNSILSAALSWANQRVLHIDENSFYGEIDGSLTLRDLEQINEKIK 61

Query: 65  SDPDRQKQVYNLSEKWFIEKESAQGETTDENKGEPRKVWSQADFAAEYRKFNIDTTPKLL 124
                Q    N S K  +++   Q              +   D   + +   I   P+ +
Sbjct: 62  KVDSSQILNDNGSHKSPLKRFEVQ--------------FLNKDLIPKNKGSVIQFHPQEI 107

Query: 125 FSRGPLVELLISSNIARYAEFRCVT--RVLTWLNDQLMPVPCSRADVFATEAVGIVEKRM 182
           F+   LV+LL  + I +Y   +     R+LT  N++ + VP SRAD+F  + + +  KR+
Sbjct: 108 FASSELVKLLSETKIYKYLLLKPARSFRLLT-SNEEWIKVPESRADIFNNKNLSLASKRI 166

Query: 183 LMKMLTSIVGYSEEEMNNEFKDWNNKTLKEYLTHK-GLTPNLIHYILYAIAGG-NNSLPC 240
           +M+ +  +   ++E+  N  K+W +K   ++L     L+  +   I+Y +    +  +P 
Sbjct: 167 VMRFMKFVSNIADEQNQNLVKEWESKPFYKFLEEVFQLSGAIEESIIYGLCQSLSKDIPT 226

Query: 241 LEGVKECKKFLMSLGRYGNTPFLWPMYGSG-ELPQCFCRLCAVFGGVYCLNRPIDKVETK 299
            + +    K+  S G YG+  +L  MYG+G EL Q FCR  AV GG + L + IDK    
Sbjct: 227 KDALDTVLKYFHSFGMYGDYSYLLAMYGTGSELCQGFCRSSAVMGGTFMLGQAIDK---- 282

Query: 300 TVDEGKTVVTIDSKSKKLNCDHLVIGVHE--CPEELMSSEPAESCDISKAVFVTNGTIMA 357
            +DE K V+        L+   +V  V E   P + +           + +F  +G   A
Sbjct: 283 -IDESKIVL---KDGSTLSAKKIVSSVDEGKLPHQQIQQRYLLVKGDCQQLFQEDGFFAA 338

Query: 358 SEKEPLTLLRFPPLADDDNAVTVLEVGPATGSCPKGLFVVYFITKKTKDAESDLKPYAER 417
            +   +    F    +  N+V     G  +G CP+G  + Y  T              ++
Sbjct: 339 LDASLIHFSPFSISENFGNSVQAKLYGSGSGDCPEGYCIWYLKTLNDSPCNEVFNLATKK 398

Query: 418 IFDMSGGD 425
           +   SG D
Sbjct: 399 LCSFSGCD 406


>SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 440

 Score =  101 bits (243), Expect = 2e-22
 Identities = 52/190 (27%), Positives = 96/190 (50%), Gaps = 7/190 (3%)

Query: 113 RKFNIDTTPKLLFSRGPLVELLISSNIARYAEFRCVTRVLTWLNDQLMPVPCSRADVFAT 172
           R + +D  PK L + G L  +LI +++ RY EF+ +     + + ++  VP +  +   +
Sbjct: 69  RDWCVDLVPKFLMANGDLTNILIYTDVTRYIEFKQIAGSYVYRDGRIAKVPGNEMEALKS 128

Query: 173 EAVGIVEKRMLMKMLTSIVGYSEEEMNNEFKDWN------NKTLKEYLTHKGLTPNLIHY 226
             + + EKR   K L  +  Y E++ +  +KD N          K++    G    + H 
Sbjct: 129 PLMSLFEKRRAKKFLEWVNNYREDDPST-YKDINIDRDSMESVFKKFGLQSGTQDFIGHA 187

Query: 227 ILYAIAGGNNSLPCLEGVKECKKFLMSLGRYGNTPFLWPMYGSGELPQCFCRLCAVFGGV 286
           +   +       P  E  +    +  S+ ++G +P+++P+YG GELPQ F RL A++GG 
Sbjct: 188 MALYLDDAYLKKPARETRERILLYASSIAKFGKSPYIYPLYGLGELPQGFARLSAIYGGT 247

Query: 287 YCLNRPIDKV 296
           Y LN+P+D++
Sbjct: 248 YMLNQPVDEI 257



 Score = 47.2 bits (107), Expect = 6e-06
 Identities = 22/60 (36%), Positives = 34/60 (56%)

Query: 9  DFQVIVVGTGMVESIVAAACSRIGKNVLHMDSSDHYGGLWASYNFEGLQKFIKEVNSDPD 68
          ++ VIV+GTG+ E +++   S  GK VLH+D +D+YG   AS N   L    +     P+
Sbjct: 4  EYDVIVLGTGLTECVLSGLLSVDGKKVLHIDRNDYYGADSASLNLTQLYALFRPGEQRPE 63


>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1687

 Score = 30.7 bits (66), Expect = 0.52
 Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 3/52 (5%)

Query: 181  RMLMKMLTSIVGYSEEEMNNEFKDWNNKTLKEYLTHKGLT--PNL-IHYILY 229
            RML+++  ++  Y E     +F D   KTL   L H G +  PNL + ++ Y
Sbjct: 1231 RMLLEVAKNVNTYCESRQQKDFIDLMGKTLSSVLFHAGKSSDPNLTVDFLAY 1282


>SPBC115.02c |||AFG1 family mitochondrial ATPase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 454

 Score = 29.1 bits (62), Expect = 1.6
 Identities = 21/79 (26%), Positives = 30/79 (37%), Gaps = 2/79 (2%)

Query: 66  DPDRQKQVYNLSEKWFIEKESAQGE--TTDENKGEPRKVWSQADFAAEYRKFNIDTTPKL 123
           DP  Q +V     K  + K S      T ++  GEP+        A+ Y  F +   PKL
Sbjct: 302 DPAHQDEVEVFGRKIIVPKASGNVAWFTFEQLCGEPKSAADYLSLASRYHVFIVSDIPKL 361

Query: 124 LFSRGPLVELLISSNIARY 142
                 L+   I+   A Y
Sbjct: 362 SIESKDLIHRFITFIDALY 380


>SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol
           3-phosphatidyltransferase Pis1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 251

 Score = 29.1 bits (62), Expect = 1.6
 Identities = 16/51 (31%), Positives = 27/51 (52%), Gaps = 1/51 (1%)

Query: 266 MYGSGELPQCFCRLCAVFGGVYCLNRPIDKVETKTVDEGKTVVTIDSKSKK 316
           +Y +GELP  +  L AV  G  CL + I  V  + V+    +V +D + ++
Sbjct: 196 IYSTGELPLSYPTLLAVLCGPICLAKQIINV-VQLVNAANALVKMDVEQRR 245


>SPAC9E9.12c |ybt1|abc1|ABC transporter Ybt1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1427

 Score = 29.1 bits (62), Expect = 1.6
 Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 3/41 (7%)

Query: 338 PAESCDISKAVFV---TNGTIMASEKEPLTLLRFPPLADDD 375
           P ++C I   +F     NG I  S K+P+TL   P L D +
Sbjct: 193 PEQTCSIFSLIFTYGWLNGIIWKSWKKPITLTDVPALPDTE 233


>SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl
          modification enzyme|Schizosaccharomyces pombe|chr
          2|||Manual
          Length = 666

 Score = 27.9 bits (59), Expect = 3.6
 Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%)

Query: 12 VIVVGTGMVESIVAAACSRIG-KNVLHMDSSDHYGGLWASYNFEGLQK 58
          V+V+G G      AAA SR+G K  L   S D+ G +  +  F G+ K
Sbjct: 19 VVVIGGGHAGVEAAAAASRLGAKTTLLTKSFDNIGQMSCNPAFGGIGK 66


>SPBC83.03c |tas3||RITS complex subunit 3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 549

 Score = 27.1 bits (57), Expect = 6.4
 Identities = 15/51 (29%), Positives = 20/51 (39%), Gaps = 2/51 (3%)

Query: 64  NSDPDRQKQVYNLSEKWFIEKE-SAQGETTDENKGEPRKVWSQADFAAEYR 113
           NSDP  + + Y     WF   +  A     D+  GE    WS  D    Y+
Sbjct: 240 NSDPQFRPK-YQAKSSWFAPDDPEASWGNLDDGWGETNGSWSSTDDTKHYK 289


>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 897

 Score = 27.1 bits (57), Expect = 6.4
 Identities = 12/21 (57%), Positives = 14/21 (66%), Gaps = 2/21 (9%)

Query: 432 KPKCLWSLFYNVKDTSSPVSS 452
           KP C+WSLF   KD S P+ S
Sbjct: 446 KPYCVWSLF--AKDISRPLKS 464


>SPBC336.02 |||18S rRNA dimethylase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 307

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 14/30 (46%), Positives = 16/30 (53%)

Query: 360 KEPLTLLRFPPLADDDNAVTVLEVGPATGS 389
           K PL        AD   + TVLEVGP TG+
Sbjct: 34  KNPLVAQGIVDKADLKQSDTVLEVGPGTGN 63


>SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 949

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 1/43 (2%)

Query: 438 SLFYNVKDTSSPVSSVVDNVHVCSGPDAGLDFDRAVEQAEQIF 480
           ++ Y++ D  +P SS+VDN  + S P      D  ++   QIF
Sbjct: 145 TITYDIDDNVNPSSSLVDNFSISSVPSVFFQSDFNLDD-PQIF 186


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.317    0.135    0.409 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,605,989
Number of Sequences: 5004
Number of extensions: 116470
Number of successful extensions: 287
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 276
Number of HSP's gapped (non-prelim): 14
length of query: 523
length of database: 2,362,478
effective HSP length: 76
effective length of query: 447
effective length of database: 1,982,174
effective search space: 886031778
effective search space used: 886031778
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 56 (26.6 bits)