Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001123-TA|BGIBMGA001123-PA|IPR001494|Importin-beta,
N-terminal, IPR013598|Exportin 1-like
         (1004 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g03110.1 68416.m00307 exportin 1, putative strong similarity ...    47   7e-05
At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to Ex...    43   0.001
At3g23080.1 68416.m02909 expressed protein weak similarity to SP...    31   2.9  
At3g08960.1 68416.m01047 importin beta-2 subunit family protein ...    30   6.7  

>At3g03110.1 68416.m00307 exportin 1, putative strong similarity to
           Exportin1 (XPO1) protein [Arabidopsis thaliana]
           GI:7671510; contains Pfam profile PF03810: Importin-beta
           N-terminal domain
          Length = 1076

 Score = 46.8 bits (106), Expect = 7e-05
 Identities = 38/166 (22%), Positives = 71/166 (42%), Gaps = 9/166 (5%)

Query: 32  TVSQDARNQAYNACESFKENSPWCAQAGLLLASGNQYSPVVKHCGLQLMEHTVKYRWTRI 91
           T S++ R  A N     K N     Q   +L   N  S   K   LQ++E  +KYRW  +
Sbjct: 28  TGSKEERASADNILRDLKANPDTWLQVVHILQ--NTSSTHTKFFALQVLEGVIKYRWNAL 85

Query: 92  TQAEKLFIKENAMKLLYMGGWEMGHLN------DALARVIVEMIKREWPQQWPTLLGELS 145
              ++  +K     ++     +           + L  ++V+++K+EWP +W + + +L 
Sbjct: 86  PVEQRDGMKNYISDVIVQLSRDEASFRTERLYVNKLNIILVQIVKQEWPAKWKSFIPDLV 145

Query: 146 DACTRGHQHTQIVLHVFLRLVEDVATLQTLEQNQRR-KDIYQALTS 190
            A        +  + +   L E+V      E  Q++ K++ Q+L S
Sbjct: 146 IAAKTSETICENCMAILKLLSEEVFDFSKGEMTQQKIKELKQSLNS 191


>At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to
           Exportin1 (XPO1) protein [Arabidopsis thaliana]
           GI:7671510
          Length = 1075

 Score = 42.7 bits (96), Expect = 0.001
 Identities = 35/166 (21%), Positives = 72/166 (43%), Gaps = 9/166 (5%)

Query: 32  TVSQDARNQAYNACESFKENSPWCAQAGLLLASGNQYSPVVKHCGLQLMEHTVKYRWTRI 91
           T S++ R  A       + N     Q   +L + N      K   LQ++E  +KYRW  +
Sbjct: 28  TGSKEERAAADQILRDLQANPDMWLQVVHILQNTNSLD--TKFFALQVLEGVIKYRWNAL 85

Query: 92  TQAEKLFIKENAMKLLY-MGGWEMGHLNDALAR-----VIVEMIKREWPQQWPTLLGELS 145
              ++  +K    +++  +   E    ++ L       ++V+++K +WP +W + + +L 
Sbjct: 86  PVEQRDGMKNYISEVIVQLSSNEASFRSERLYVNKLNVILVQIVKHDWPAKWTSFIPDLV 145

Query: 146 DACTRGHQHTQIVLHVFLRLVEDVATLQTLEQNQRR-KDIYQALTS 190
            A        +  + +   L E+V      E  Q++ K++ Q+L S
Sbjct: 146 AAAKTSETICENCMAILKLLSEEVFDFSRGEMTQQKIKELKQSLNS 191


>At3g23080.1 68416.m02909 expressed protein weak similarity to
           SP|Q9UKL6 Phosphatidylcholine transfer protein (PC-TP)
           {Homo sapiens}
          Length = 419

 Score = 31.5 bits (68), Expect = 2.9
 Identities = 15/49 (30%), Positives = 23/49 (46%)

Query: 504 AYSQLAGPGVGAAGAELLPRVLDKIFSSLVYEGAAPDQRRSRAVKNVRR 552
           AY     PG   + +  +  +  K+   LV    A D+ R RAV+N R+
Sbjct: 328 AYQSARKPGTSLSRSAQMASITTKLNMDLVETSGAEDEERGRAVENARK 376


>At3g08960.1 68416.m01047 importin beta-2 subunit family protein low
           similarity to Lph2p [Saccharomyces cerevisiae]
           GI:1163089; contains Pfam profile PF03810: Importin-beta
           N-terminal domain
          Length = 754

 Score = 30.3 bits (65), Expect = 6.7
 Identities = 37/167 (22%), Positives = 70/167 (41%), Gaps = 9/167 (5%)

Query: 14  VALIADELSRAVELTLNPTVSQDARNQAYNACESFKENSP-WCAQAGLLLASGNQYSPV- 71
           +AL A +L     L  N     +   +   A  S  E+ P +C+    ++AS +  S V 
Sbjct: 1   MALSASDLPAMYTLLANSMSGDETVRRPAEAALSLSESRPGFCSCLMEVIASKDLVSHVD 60

Query: 72  VKHCGLQLMEHTVKYRWTRITQAEKLFIKENA---MKLLYMGGWEMGHLNDALARVIVEM 128
           V+       ++++   W     +  +  +E +    KLL     E   + + LA +I ++
Sbjct: 61  VRLMASVYFKNSINRHWKSRRNSWSMSNEEKSHLRQKLLSHLREENYQIAEMLAVLISKI 120

Query: 129 IKREWPQQWPTLLGELSDACTRGHQHTQIVLH-VFLRLVEDVATLQT 174
            + ++P++WP L   L+      H    +  H +FL L   +  L T
Sbjct: 121 ARFDYPREWPDLFSVLAQQL---HSADVLASHRIFLILFRTLKELST 164


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.319    0.132    0.392 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,900,655
Number of Sequences: 28952
Number of extensions: 677546
Number of successful extensions: 1613
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1607
Number of HSP's gapped (non-prelim): 5
length of query: 1004
length of database: 12,070,560
effective HSP length: 88
effective length of query: 916
effective length of database: 9,522,784
effective search space: 8722870144
effective search space used: 8722870144
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 64 (29.9 bits)