Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001115-TA|BGIBMGA001115-PA|IPR001173|Glycosyl
transferase, family 2
         (335 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g39630.1 68415.m04858 glycosyl transferase family 2 protein s...   236   2e-62
At2g39630.2 68415.m04859 glycosyl transferase family 2 protein s...   120   1e-27
At1g20575.1 68414.m02567 dolichyl-phosphate beta-D-mannosyltrans...    58   6e-09
At2g17870.1 68415.m02070 cold-shock DNA-binding family protein c...    30   1.9  
At5g62480.2 68418.m07842 glutathione S-transferase, putative           29   4.4  
At5g62480.1 68418.m07841 glutathione S-transferase, putative           29   4.4  
At5g44316.1 68418.m05426 ATP-binding-cassette transporter, putat...    29   4.4  
At5g05030.1 68418.m00534 expressed protein contains similarity t...    28   7.7  
At4g20160.1 68417.m02949 expressed protein ; expression supporte...    28   7.7  
At3g20480.1 68416.m02594 tetraacyldisaccharide 4'-kinase family ...    28   7.7  
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ...    28   7.7  
At1g05020.1 68414.m00503 epsin N-terminal homology (ENTH) domain...    28   7.7  

>At2g39630.1 68415.m04858 glycosyl transferase family 2 protein
           similar to dolichyl-phosphate beta-glucosyltransferase
           from Saccharomyces cerevisiae [SP|P40350]; contains Pfam
           glycosyltransferase group 2 domain PF00535
          Length = 336

 Score =  236 bits (577), Expect = 2e-62
 Identities = 123/262 (46%), Positives = 174/262 (66%), Gaps = 8/262 (3%)

Query: 75  PAYNEEKRLPPMLDETIEFLENRQKENPSYKYEIIIVSDGSKDSTVKVAESYSIKYGSDK 134
           PAYNEE RLP  L+ET+++L++R   + S+ +E++IV DGS D T +VA  +  KY  D 
Sbjct: 73  PAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRVAFDFIRKYTIDN 132

Query: 135 VKCLELIKNRGKGGAVRLGIQSSRGATILFADADGASKFEDLTKLE-----VALKDIVKC 189
           ++ + L KN+GKG A+R G+  SRG  +L  DADGA+K  DL KLE     VA ++    
Sbjct: 133 IRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQINAVAREEYSIR 192

Query: 190 DPL-KDVKTTSESLGI-VIGSRAHLEKESLAKRNIFRNILMYGFHFLVWLFTVKGIKDTQ 247
           +P  KD+      + +   GSRAHLE+++LA R  +RN LM GFH +V L    GI+DTQ
Sbjct: 193 NPASKDMDFKIGDVQVSAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLAAGPGIRDTQ 252

Query: 248 CGFKLFTRKAARICFESLHVNRWAFDVELLYIAQKLNIPISEIPVRWTEIEGSKVTPVVA 307
           CGFK+FTR AAR  F ++H+ RW FDVEL+Y+ ++ NIP+ EI V+W+EI GSKV+ +++
Sbjct: 253 CGFKMFTRAAARRLFTNVHLKRWCFDVELVYLCKRFNIPMVEISVKWSEIPGSKVS-MLS 311

Query: 308 WIQMGCDLGLIWLKYRIGAWKI 329
              M  +L L+ + YR G WKI
Sbjct: 312 IPNMLWELALMSVGYRTGMWKI 333


>At2g39630.2 68415.m04859 glycosyl transferase family 2 protein
           similar to dolichyl-phosphate beta-glucosyltransferase
           from Saccharomyces cerevisiae [SP|P40350]; contains Pfam
           glycosyltransferase group 2 domain PF00535
          Length = 236

 Score =  120 bits (289), Expect = 1e-27
 Identities = 68/157 (43%), Positives = 98/157 (62%), Gaps = 7/157 (4%)

Query: 75  PAYNEEKRLPPMLDETIEFLENRQKENPSYKYEIIIVSDGSKDSTVKVAESYSIKYGSDK 134
           PAYNEE RLP  L+ET+++L++R   + S+ +E++IV DGS D T +VA  +  KY  D 
Sbjct: 73  PAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRVAFDFIRKYTIDN 132

Query: 135 VKCLELIKNRGKGGAVRLGIQSSRGATILFADADGASKFEDLTKLE-----VALKDIVKC 189
           ++ + L KN+GKG A+R G+  SRG  +L  DADGA+K  DL KLE     VA ++    
Sbjct: 133 IRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQINAVAREEYSIR 192

Query: 190 DPL-KDVKTTSESLGI-VIGSRAHLEKESLAKRNIFR 224
           +P  KD+      + +   GSRAHLE+++LA    FR
Sbjct: 193 NPASKDMDFKIGDVQVSAFGSRAHLEEKALATSFNFR 229


>At1g20575.1 68414.m02567 dolichyl-phosphate
           beta-D-mannosyltransferase, putative /
           dolichol-phosphate mannosyltransferase, putative /
           mannose-P-dolichol synthase, putative similar to DPM1
           from Homo sapiens [SP|O60762]; member of
           glycosyltransferase family 2
          Length = 246

 Score = 58.4 bits (135), Expect = 6e-09
 Identities = 46/191 (24%), Positives = 84/191 (43%), Gaps = 15/191 (7%)

Query: 106 YEIIIVSDGSKDSTVKVAESYSIKYGSDKVKCLELIKNRGKGGAVRLGIQSSRGATILFA 165
           +EII+V DGS D T ++ +     YG D++      K  G G A   G++ + G  ++  
Sbjct: 44  FEIIVVDDGSPDGTQEIVKQLQQLYGEDRILLRARAKKLGLGTAYIHGLKHATGDFVVIM 103

Query: 166 DADGASKFEDLTKLEVALKDIVKCDPLKDVKTTSESLGIVIGSRAHLEKESLAKRNIFRN 225
           DA       DL+     L   +K    K ++T +    IV G+R +++   +   N+ R 
Sbjct: 104 DA-------DLSHHPKYLPSFIK----KQLETNA---SIVTGTR-YVKGGGVHGWNLMRK 148

Query: 226 ILMYGFHFLVWLFTVKGIKDTQCGFKLFTRKAARICFESLHVNRWAFDVELLYIAQKLNI 285
           +   G + L       G+ D    F+L+ + A      S     + F +E++  A +   
Sbjct: 149 LTSRGANVLAQTLLWPGVSDLTGSFRLYKKSALEDVISSCVSKGYVFQMEMIVRATRKGY 208

Query: 286 PISEIPVRWTE 296
            I E+P+ + +
Sbjct: 209 HIEEVPITFVD 219


>At2g17870.1 68415.m02070 cold-shock DNA-binding family protein
           contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
           domain and PF00098: Zinc knuckle
          Length = 301

 Score = 30.3 bits (65), Expect = 1.9
 Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 3/58 (5%)

Query: 104 YKYEIIIVSDGSKDSTVKVAESYSIKYGSD-KVKCLELIKNRGKGGAVRLGIQSSRGA 160
           + ++  IVSDG +  T+  +  Y I  GSD K K +E+      GG++     SSRG+
Sbjct: 37  FVHQSSIVSDGFRSLTLGESVEYEIALGSDGKTKAIEV--TAPGGGSLNKKENSSRGS 92


>At5g62480.2 68418.m07842 glutathione S-transferase, putative
          Length = 214

 Score = 29.1 bits (62), Expect = 4.4
 Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 3/56 (5%)

Query: 151 RLGIQSSRGATILFADADGASKFEDLTKLEVALKDIVKCDPLKDVKTTSESLGIVI 206
           +L +    G   +F+D DG      +T   ++L DIV C  L   K   E LG+ I
Sbjct: 109 KLSVIEKEGLKEIFSDTDGEPT---VTNETMSLVDIVMCTLLSPYKAHEEVLGLKI 161


>At5g62480.1 68418.m07841 glutathione S-transferase, putative
          Length = 240

 Score = 29.1 bits (62), Expect = 4.4
 Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 3/56 (5%)

Query: 151 RLGIQSSRGATILFADADGASKFEDLTKLEVALKDIVKCDPLKDVKTTSESLGIVI 206
           +L +    G   +F+D DG      +T   ++L DIV C  L   K   E LG+ I
Sbjct: 135 KLSVIEKEGLKEIFSDTDGEPT---VTNETMSLVDIVMCTLLSPYKAHEEVLGLKI 187


>At5g44316.1 68418.m05426 ATP-binding-cassette transporter, putative
           similar to ATP-binding-cassette transporter (ABC1)
           described in PMID:11156608
          Length = 470

 Score = 29.1 bits (62), Expect = 4.4
 Identities = 19/64 (29%), Positives = 27/64 (42%)

Query: 166 DADGASKFEDLTKLEVALKDIVKCDPLKDVKTTSESLGIVIGSRAHLEKESLAKRNIFRN 225
           +AD     E+  +L+V L    KC P   V     S  I I ++  LEK  +   +I   
Sbjct: 160 EADDPKLLEEFDRLDVPLTKKKKCSPKVAVDAVYNSESIAITNKEPLEKSGVIFCSISEA 219

Query: 226 ILMY 229
           I  Y
Sbjct: 220 IRKY 223


>At5g05030.1 68418.m00534 expressed protein contains similarity to
           carboxyl-terminal proteinase contains Pfam profile
           PF03080: Arabidopsis proteins of unknown function
          Length = 363

 Score = 28.3 bits (60), Expect = 7.7
 Identities = 17/41 (41%), Positives = 20/41 (48%)

Query: 156 SSRGATILFADADGASKFEDLTKLEVALKDIVKCDPLKDVK 196
           S+R   + F D D   K  D  KLE  + D  KC  LKD K
Sbjct: 306 SARVGEVKFIDNDFVVKGSDQYKLEKLIDDSNKCYGLKDGK 346


>At4g20160.1 68417.m02949 expressed protein ; expression supported
           by MPSS
          Length = 1188

 Score = 28.3 bits (60), Expect = 7.7
 Identities = 14/42 (33%), Positives = 21/42 (50%)

Query: 147 GGAVRLGIQSSRGATILFADADGASKFEDLTKLEVALKDIVK 188
           G  V +  + S G T   A+  G  K EDL  +E   KD+++
Sbjct: 706 GDRVIMDKRKSSGETTRSAEIGGGKKEEDLASVEAKSKDVIE 747


>At3g20480.1 68416.m02594 tetraacyldisaccharide 4'-kinase family
           protein contains Pfam PF02606: tetraacyldisaccharide-1-P
           4'-kinase
          Length = 395

 Score = 28.3 bits (60), Expect = 7.7
 Identities = 17/40 (42%), Positives = 24/40 (60%), Gaps = 4/40 (10%)

Query: 131 GSDKVKCLELIKNRGKGGAVRLGIQSSRGAT-ILFADADG 169
           G D+VK LE      +GG V++G+ ++R AT  LF D  G
Sbjct: 103 GGDEVKMLE---RHLRGGPVKIGVGANRAATAALFLDKYG 139


>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
           identical to GB:AF043519
          Length = 246

 Score = 28.3 bits (60), Expect = 7.7
 Identities = 9/19 (47%), Positives = 14/19 (73%)

Query: 89  ETIEFLENRQKENPSYKYE 107
           E + FLE + KENP++ Y+
Sbjct: 174 EAVNFLEKKMKENPAFTYD 192


>At1g05020.1 68414.m00503 epsin N-terminal homology (ENTH)
           domain-containing protein / clathrin assembly
           protein-related Similar to clathrin assembly protein
           gb|X68878 (AP180) from Rattus norvegicus;  contains Pfam
           PF01417: ENTH domain. ENTH (Epsin N-terminal homology)
           domain;  EST gb|W43552 comes from this gene
          Length = 653

 Score = 28.3 bits (60), Expect = 7.7
 Identities = 18/48 (37%), Positives = 27/48 (56%), Gaps = 4/48 (8%)

Query: 167 ADGASKFEDLTKLEVALKDIVKCD---PLKDVKTTSESLGIVIGSRAH 211
           A+GA+   DLT LEVA+      D   P+ D +  +E LGI+   ++H
Sbjct: 24  ANGATGGGDLTTLEVAILKATSHDEEVPIDD-RLVTEILGIISSKKSH 70


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.320    0.137    0.407 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,132,986
Number of Sequences: 28952
Number of extensions: 278379
Number of successful extensions: 679
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 668
Number of HSP's gapped (non-prelim): 12
length of query: 335
length of database: 12,070,560
effective HSP length: 81
effective length of query: 254
effective length of database: 9,725,448
effective search space: 2470263792
effective search space used: 2470263792
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 60 (28.3 bits)