BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001107-TA|BGIBMGA001107-PA|IPR002048|Calcium-binding EF-hand (322 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 32 0.095 SPAC1D4.01 ||SPAC1F3.11|sequence orphan|Schizosaccharomyces pomb... 30 0.51 SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 29 0.89 SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 28 1.5 SPAC8C9.10c |||ribosome biogenesis protein Rrp14-N|Schizosacchar... 28 2.0 SPBC776.17 |||rRNA processing protein Rrp7 |Schizosaccharomyces ... 28 2.0 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.7 SPBC543.02c |||DNAJ/TPR domain protein DNAJC7 family|Schizosacch... 27 2.7 SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|c... 27 2.7 SPAC19B12.05c |fcp1||CTD phosphatase Fcp1 |Schizosaccharomyces p... 27 4.7 SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pomb... 26 6.2 SPBP35G2.09 |usp103|yhc1|U1 snRNP-associated protein Usp103 |Sch... 26 6.2 SPBC32H8.08c |||mannosyltransferase complex subunit |Schizosacch... 26 8.3 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 26 8.3 SPBC13G1.08c |ash2||Ash2-trithorax family protein|Schizosaccharo... 26 8.3 SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr... 26 8.3 SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr 2... 26 8.3 SPAC17H9.10c |ddb1||damaged DNA binding protein Ddb1 |Schizosacc... 26 8.3 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 32.3 bits (70), Expect = 0.095 Identities = 20/85 (23%), Positives = 45/85 (52%), Gaps = 4/85 (4%) Query: 138 DMDEKELKAPNSEGFTYSNLQKRDRRRWTYADADQNDALNRTEFAAFLHPEDHSSMRD-- 195 D+D +E S + N +++ + + D D++ ++ E L +++R+ Sbjct: 72 DVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQ 131 Query: 196 --VVVLETLEDIDKDQDGKVSLDEY 218 +V +T+ ++DKD+DGK+S +E+ Sbjct: 132 LQQIVDKTIMEVDKDRDGKISFEEF 156 Score = 28.3 bits (60), Expect = 1.5 Identities = 34/154 (22%), Positives = 65/154 (42%), Gaps = 19/154 (12%) Query: 153 TYSNLQ-KRDRRRWTYADADQNDALNRTEFAAFLHPEDHSSMRDVVVLETLEDIDKDQDG 211 ++SN + +R R+R+ DA+Q+ +++R EF + S + + +D+D G Sbjct: 17 SFSNEEIERIRKRFIKIDANQSGSIDRNEFLSI-----PSVASNPLASRLFSVVDEDGGG 71 Query: 212 KVSLDEYIGDMYKXXXXXXXXXXXWVKQEREQFT-GYRDTNKDGFMDEHE----VKDWIA 266 V E+I + K+E+ +F D ++DG++ E +K + Sbjct: 72 DVDFQEFINSL-------SVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVG 124 Query: 267 PP-EFDHAEAEARHLVFEADADADEKLTKAEIID 299 D + + E D D D K++ E D Sbjct: 125 TNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKD 158 >SPAC1D4.01 ||SPAC1F3.11|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 29.9 bits (64), Expect = 0.51 Identities = 15/54 (27%), Positives = 27/54 (50%), Gaps = 6/54 (11%) Query: 159 KRDRRRWTYADADQNDALNRTEFAAF------LHPEDHSSMRDVVVLETLEDID 206 K++R Y++ + +ALN + LHP +HS +RD L + ++D Sbjct: 158 KQERLAQNYSENGETNALNTKNESTVQNIKNSLHPNEHSFIRDAAALGAIREVD 211 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 29.1 bits (62), Expect = 0.89 Identities = 14/49 (28%), Positives = 26/49 (53%) Query: 169 DADQNDALNRTEFAAFLHPEDHSSMRDVVVLETLEDIDKDQDGKVSLDE 217 DAD N ++ TEF + + + + V E + DKD +G ++++E Sbjct: 58 DADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEE 106 Score = 26.6 bits (56), Expect = 4.7 Identities = 13/69 (18%), Positives = 33/69 (47%) Query: 62 QLSPEESKRRLGEIADKIDSDQDGFITLVELKDWIRYTQKRYIDEDVERHWRQQNPNNEE 121 ++ +++ + E D D +G+IT+ EL + +R E+V R+ + + + Sbjct: 77 KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDG 136 Query: 122 FVTWEAYRK 130 + +E + + Sbjct: 137 VINYEEFSR 145 >SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 28.3 bits (60), Expect = 1.5 Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 1/59 (1%) Query: 163 RRWTYADADQNDALNRTEFAAFLHPEDHSSMRDVVVLETLEDIDKDQDGKVSLDEYIGD 221 RRW + + AL R E A +++ + E L D D++ +VS DEY D Sbjct: 415 RRWMNREKARTAALER-EAAREEEERVNNTSFGTYLSEKLASFDDDEEARVSRDEYFVD 472 >SPAC8C9.10c |||ribosome biogenesis protein Rrp14-N|Schizosaccharomyces pombe|chr 1|||Manual Length = 154 Score = 27.9 bits (59), Expect = 2.0 Identities = 20/77 (25%), Positives = 35/77 (45%), Gaps = 5/77 (6%) Query: 74 EIADKIDSDQDGFITLVELKDWIRYTQKRYIDEDVERHWRQQNPNNEEFVTWEAYRKNVY 133 E+ ++I++ DGF EL +I K Y E W+Q+ EE + ++ Sbjct: 3 EVEERINAWNDGF---EELLSYI--PAKLYYREHTANQWKQKKSTLEEKKERRKMKFSLD 57 Query: 134 GFMDDMDEKELKAPNSE 150 G ++D D+ K+ E Sbjct: 58 GLVNDADDDSQKSSKWE 74 >SPBC776.17 |||rRNA processing protein Rrp7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 254 Score = 27.9 bits (59), Expect = 2.0 Identities = 18/53 (33%), Positives = 29/53 (54%), Gaps = 4/53 (7%) Query: 40 EHHNKQFDHDAFLGEDQAKTFDQLSPEE--SKRRLGEIADKIDSDQDGFITLV 90 E +++QF + L E +++ +E KRRL A K D+DGF+T+V Sbjct: 136 ESYDRQFVNPRVLAESVDSYMKRVTKKEIAEKRRL--FAMKNQPDEDGFVTVV 186 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 27.5 bits (58), Expect = 2.7 Identities = 18/62 (29%), Positives = 27/62 (43%), Gaps = 2/62 (3%) Query: 18 VPTNEETKRLMDHLSDAEHYRNEHHNKQFDHDAFLGEDQAKTFDQLSPEESKRRLGEIAD 77 V E + R+ LSD +H F ED ++DQ SPE ++ R I+ Sbjct: 1061 VRLEELSTRVSKMLSDINEV--DHTIASLSLKLFQAEDTKNSYDQTSPEATQERNRTISS 1118 Query: 78 KI 79 K+ Sbjct: 1119 KL 1120 >SPBC543.02c |||DNAJ/TPR domain protein DNAJC7 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 476 Score = 27.5 bits (58), Expect = 2.7 Identities = 18/92 (19%), Positives = 39/92 (42%) Query: 52 LGEDQAKTFDQLSPEESKRRLGEIADKIDSDQDGFITLVELKDWIRYTQKRYIDEDVERH 111 +GE + D + K + + +I +G L E + +++ Q ++R Sbjct: 72 IGEFELALCDAKQSDRIKPDVPKTQSRIRQAYEGLSILNEAEVYLKNKQAGLALNALDRL 131 Query: 112 WRQQNPNNEEFVTWEAYRKNVYGFMDDMDEKE 143 R+ + + ++W + VY F +DMD + Sbjct: 132 QRRIDSTTQPPMSWMYLKAQVYIFQNDMDRAQ 163 >SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 27.5 bits (58), Expect = 2.7 Identities = 15/58 (25%), Positives = 30/58 (51%), Gaps = 3/58 (5%) Query: 25 KRLMDHLSDAEHYRNEHHNKQFDHDAFLGEDQAK---TFDQLSPEESKRRLGEIADKI 79 +RL +HL +++ +H NK+ D + E++ K ++ PE K+ G +K+ Sbjct: 179 QRLAEHLENSKKTILQHDNKEEDKEIHSEENETKDEIKSEKKEPEIKKQEGGSSTEKV 236 >SPAC19B12.05c |fcp1||CTD phosphatase Fcp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 723 Score = 26.6 bits (56), Expect = 4.7 Identities = 19/75 (25%), Positives = 31/75 (41%) Query: 140 DEKELKAPNSEGFTYSNLQKRDRRRWTYADADQNDALNRTEFAAFLHPEDHSSMRDVVVL 199 + + L+ N E T LQK +R +A D T LH DH R VL Sbjct: 394 NREALEEQNKERVTALELQKSERPLAKQQNALLEDEGKPTPSHTLLHNRDHELERLEKVL 453 Query: 200 ETLEDIDKDQDGKVS 214 + + + +++ +S Sbjct: 454 KDIHAVYYEEENDIS 468 >SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1095 Score = 26.2 bits (55), Expect = 6.2 Identities = 9/31 (29%), Positives = 18/31 (58%) Query: 257 DEHEVKDWIAPPEFDHAEAEARHLVFEADAD 287 + E+++W+ + D E++A H+V E D Sbjct: 327 EAEELQEWLNTDDLDQDESDANHVVAEQAMD 357 >SPBP35G2.09 |usp103|yhc1|U1 snRNP-associated protein Usp103 |Schizosaccharomyces pombe|chr 2|||Manual Length = 182 Score = 26.2 bits (55), Expect = 6.2 Identities = 10/35 (28%), Positives = 15/35 (42%) Query: 18 VPTNEETKRLMDHLSDAEHYRNEHHNKQFDHDAFL 52 +P E +R H + H+ HH Q H F+ Sbjct: 146 LPQTNEHQRARTHSNANNHFTKTHHQGQRSHQRFV 180 >SPBC32H8.08c |||mannosyltransferase complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 25.8 bits (54), Expect = 8.3 Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 1/67 (1%) Query: 155 SNLQKRDRRRWTYADADQNDALNRTEFAAFLHPEDHSSMRDVVVLETLEDIDKDQDGKVS 214 +++++R R + Y ND TEF + SS++ V+ + L + KD D K Sbjct: 122 NSIERRFNRHFKYPYVFLNDEPFTTEFKKAVKDATDSSIQFGVLDDELWNFPKDVD-KDM 180 Query: 215 LDEYIGD 221 +DE I + Sbjct: 181 IDESIAE 187 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 25.8 bits (54), Expect = 8.3 Identities = 12/59 (20%), Positives = 28/59 (47%) Query: 61 DQLSPEESKRRLGEIADKIDSDQDGFITLVELKDWIRYTQKRYIDEDVERHWRQQNPNN 119 ++L ES+ + D D G+I + D+++ ++ D +V+ ++ +P N Sbjct: 69 NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTN 127 >SPBC13G1.08c |ash2||Ash2-trithorax family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 652 Score = 25.8 bits (54), Expect = 8.3 Identities = 8/20 (40%), Positives = 13/20 (65%) Query: 108 VERHWRQQNPNNEEFVTWEA 127 +E HW+ P+ E+ TW+A Sbjct: 159 IEEHWQLLCPDREKVQTWQA 178 >SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochromatin assembly Hrr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1015 Score = 25.8 bits (54), Expect = 8.3 Identities = 12/56 (21%), Positives = 23/56 (41%) Query: 125 WEAYRKNVYGFMDDMDEKELKAPNSEGFTYSNLQKRDRRRWTYADADQNDALNRTE 180 WE KN D++ E E + YSN+ D ++ + + ++T+ Sbjct: 17 WEMVNKNTEVVFDEIPEPETLSEMKRHPLYSNIFNADNNTTSFTEQIETSETSKTQ 72 >SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 202 Score = 25.8 bits (54), Expect = 8.3 Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Query: 120 EEFVTWEAYRKNVYGFMDDMDEKELKAPNSEGFTYSNLQ 158 E+FV W + N+YG M +EL+A N + +LQ Sbjct: 82 EKFVEWAVFSGNMYG-TSIMAIQELEAVNKKAILDIDLQ 119 >SPAC17H9.10c |ddb1||damaged DNA binding protein Ddb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1072 Score = 25.8 bits (54), Expect = 8.3 Identities = 12/47 (25%), Positives = 23/47 (48%), Gaps = 1/47 (2%) Query: 157 LQKRDRRRWTYADADQNDALNRTEFAAFLHPEDHSSMRDVVVLETLE 203 L R + RW Y + +N+T F+ P+D S + ++ T++ Sbjct: 925 LSDRKKLRW-YKKFYLGELINKTRHCTFIEPQDKSLVTPQLLCATVD 970 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.317 0.134 0.398 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,610,502 Number of Sequences: 5004 Number of extensions: 74654 Number of successful extensions: 232 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 217 Number of HSP's gapped (non-prelim): 21 length of query: 322 length of database: 2,362,478 effective HSP length: 73 effective length of query: 249 effective length of database: 1,997,186 effective search space: 497299314 effective search space used: 497299314 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 54 (25.8 bits)
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