Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001102-TA|BGIBMGA001102-PA|IPR013234|PIGA, GPI anchor
biosynthesis, IPR001296|Glycosyl transferase, group 1
         (431 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC3D6.07 |gpi3||pig-A|Schizosaccharomyces pombe|chr 2|||Manual      432   e-122
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch...    38   0.003
SPAP27G11.05c |vps41||vacuolar protein sorting-associated protei...    28   2.2  
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos...    28   2.9  
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb...    27   6.7  
SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3' ss-tail|S...    27   6.7  
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc...    27   6.7  
SPAC1296.06 |||NADPH cytochrome reductase|Schizosaccharomyces po...    26   8.9  

>SPBC3D6.07 |gpi3||pig-A|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 456

 Score =  432 bits (1064), Expect = e-122
 Identities = 207/420 (49%), Positives = 284/420 (67%), Gaps = 4/420 (0%)

Query: 12  MASDFFYPNTGGVEEHIFNLSQCLIKRGHKVIVLTHSYGDRVGIRYLTAGLKVYYLPIRV 71
           M SDFF+P  GG+E HIF LSQ LI  GHKVIV+TH+Y DRVG+RYLT GL VYY+P+  
Sbjct: 1   MVSDFFFPQPGGIESHIFQLSQRLIDLGHKVIVITHAYKDRVGVRYLTNGLTVYYVPLHT 60

Query: 72  FYAQCVLPTMICNIALIRNILIRECIEIVHGHSAFSVLCHEVCIIGKLMGLKTVFTDHSL 131
            Y +   P+      + RNI+IRE IEIVHGH + S LCH+  +  + MGLKT FTDHSL
Sbjct: 61  VYRETTFPSFFSFFPIFRNIVIRENIEIVHGHGSLSFLCHDAILHARTMGLKTCFTDHSL 120

Query: 132 FGFADTSAVLTNKYLQICLSEIDHCICVSHTGKENTVLRAKVQAHKVSVIPNAVDAFSFI 191
           FGFAD  +++TNK L+  +S+++H ICVSHT +ENTVLRA +   +VSVIPNA+ A +F 
Sbjct: 121 FGFADAGSIVTNKLLKFTMSDVNHVICVSHTCRENTVLRAVLNPKRVSVIPNALVAENFQ 180

Query: 192 PDPCQRDQKFITIVIVSRLVYRKGVNLMAAVIADMCPRYPNLRFIIGGDGPKMWLLQEVR 251
           PDP +  + F+TIV++SRL Y KG++L+ AVI  +C ++P +RF+I GDGPK   L+++R
Sbjct: 181 PDPSKASKDFLTIVVISRLYYNKGIDLLIAVIPRICAQHPKVRFVIAGDGPKSIDLEQMR 240

Query: 252 EKIGCQESVRLLGSLKHSEIRNVLVKGDIFLNTSLTEAYCMAIVEAAACGLKVVSTKVGG 311
           EK   Q+ V +LGS++H ++R+V+V+G I+L+ SLTEA+   +VEAA+CGL V+STKVGG
Sbjct: 241 EKYMLQDRVEMLGSVRHDQVRDVMVRGHIYLHPSLTEAFGTVLVEAASCGLYVISTKVGG 300

Query: 312 IPEVLPESMIYLTEPNVGSLVRGIEKAITDIKEGNIMCPFKCNRLVREMYSWMDITKRTE 371
           +PEVLP  M     P    L   +   ITD  +  I      +  V++MYSW+D+ +RTE
Sbjct: 301 VPEVLPSHMTRFARPEEDDLADTLSSVITDYLDHKIKTE-TFHEEVKQMYSWIDVAERTE 359

Query: 372 GVYDRILLNKNKPLGQQLRSYLNSGVWP---FLLVISLMYILLQLTERIYKRKYIDKARD 428
            VYD I    N  L  +L+ Y   G W    F L+I++ Y+++ L E I+    ID A D
Sbjct: 360 KVYDSICSENNLRLIDRLKLYYGCGQWAGKLFCLLIAIDYLVMVLLEWIWPASDIDPAVD 419


>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
            Mok12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2352

 Score = 37.9 bits (84), Expect = 0.003
 Identities = 36/146 (24%), Positives = 63/146 (43%), Gaps = 5/146 (3%)

Query: 204  IVIVSRLVYRKGVNLMAAVIADMCPRYPNLRFIIGGDGPKMW--LLQEVREKIGCQESVR 261
            +V V R  ++KG++L+A +   +   + N++ I  G    +      E  E+I  +   R
Sbjct: 1467 LVFVGRWSHQKGIDLIADLAPKLLTEH-NVQLITIGPVIDLHGQFAAEKLEQIAKRFPTR 1525

Query: 262  LLGSLKHSEIRNVLVKG-DIFLNTSLTEAYCMAIVEAAACGLKVVSTKVGGIPEVLPESM 320
            +L     + +   L  G D  L  S  E + +  VE    G+  + ++ GG+   +P   
Sbjct: 1526 VLCKPVFTAVPPFLFAGTDFALIPSRDEPFGLVAVEFGRKGVLCIGSRTGGLGH-MPGWW 1584

Query: 321  IYLTEPNVGSLVRGIEKAITDIKEGN 346
              +  PN G L+   E AIT     N
Sbjct: 1585 FQMASPNTGHLLTQFENAITKALHSN 1610


>SPAP27G11.05c |vps41||vacuolar protein sorting-associated protein
           Vps41|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 886

 Score = 28.3 bits (60), Expect = 2.2
 Identities = 21/72 (29%), Positives = 30/72 (41%), Gaps = 5/72 (6%)

Query: 143 NKYLQICLSEIDHCICVSHTG-----KENTVLRAKVQAHKVSVIPNAVDAFSFIPDPCQR 197
           N  +  C    +H    SH G     ++N +L  K+  H  SV+  +VD  S     C  
Sbjct: 41  NDTISACAISKEHFFFGSHNGAIYIYQKNGILLRKMILHSASVVDLSVDLESENLASCSM 100

Query: 198 DQKFITIVIVSR 209
           D K I   I +R
Sbjct: 101 DGKMIISNITTR 112


>SPBC119.07 |ppk19||serine/threonine protein kinase
            Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1706

 Score = 27.9 bits (59), Expect = 2.9
 Identities = 11/45 (24%), Positives = 23/45 (51%)

Query: 341  DIKEGNIMCPFKCNRLVREMYSWMDITKRTEGVYDRILLNKNKPL 385
            D++ G + C +     + +M+  +D+TK+   V +    N N P+
Sbjct: 1398 DLRFGTLSCSWHMPARIDQMHLLLDVTKKRSNVNEYTSGNNNSPV 1442


>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 605

 Score = 26.6 bits (56), Expect = 6.7
 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%)

Query: 88  IRNILIRECIEIVHGHSAFSVLC 110
           I N    ECI I+HGH+  SVLC
Sbjct: 337 IWNYRTSECISILHGHTD-SVLC 358


>SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3'
           ss-tail|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 957

 Score = 26.6 bits (56), Expect = 6.7
 Identities = 15/66 (22%), Positives = 31/66 (46%)

Query: 319 SMIYLTEPNVGSLVRGIEKAITDIKEGNIMCPFKCNRLVREMYSWMDITKRTEGVYDRIL 378
           S + L  PNV   ++ +++ ++D+   + + PF    L +      D+++     + RIL
Sbjct: 212 SRVLLRCPNVFKKLKEVQQPLSDVSVPSDVLPFTIKLLKKISQRAQDLSRSHRNEFLRIL 271

Query: 379 LNKNKP 384
              N P
Sbjct: 272 TEFNIP 277


>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 587

 Score = 26.6 bits (56), Expect = 6.7
 Identities = 14/44 (31%), Positives = 25/44 (56%)

Query: 230 YPNLRFIIGGDGPKMWLLQEVREKIGCQESVRLLGSLKHSEIRN 273
           YP + FI    GP+   L+++  K G + ++R  GS+K  + R+
Sbjct: 197 YPEINFIGLLIGPRGHTLKDMEAKSGAKIAIRGKGSVKEGKGRS 240


>SPAC1296.06 |||NADPH cytochrome reductase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 558

 Score = 26.2 bits (55), Expect = 8.9
 Identities = 18/79 (22%), Positives = 32/79 (40%), Gaps = 1/79 (1%)

Query: 289 AYCMAIVEAAACGLKVVSTKVGGIPEVLPESMIYLTEPNVGSLVRGIE-KAITDIKEGNI 347
           AY    + +    +K  S    G  ++LP S   + + ++G  V+G E   I     G I
Sbjct: 117 AYWCNHLYSQLAAIKTPSRPAFGEFDLLPPSFQIIIDESLGCKVKGFEDNNIVRHSRGKI 176

Query: 348 MCPFKCNRLVREMYSWMDI 366
                 N+ +  +  W D+
Sbjct: 177 EATLVHNKRISNIKHWQDV 195


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.327    0.142    0.431 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,895,028
Number of Sequences: 5004
Number of extensions: 76903
Number of successful extensions: 221
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 217
Number of HSP's gapped (non-prelim): 9
length of query: 431
length of database: 2,362,478
effective HSP length: 75
effective length of query: 356
effective length of database: 1,987,178
effective search space: 707435368
effective search space used: 707435368
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.7 bits)
S2: 55 (26.2 bits)