Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001093-TA|BGIBMGA001093-PA|IPR003698|Lipoate synthase,
IPR007197|Radical SAM, IPR006638|Elongator protein 3/MiaB/NifB
         (366 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccha...   419   e-118
SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch...    31   0.26 
SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pomb...    26   7.3  
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy...    26   9.7  
SPBC14C8.11c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    26   9.7  

>SPBC8D2.15 |||mitochondrial lipoic acid synthetase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 370

 Score =  419 bits (1031), Expect = e-118
 Identities = 204/337 (60%), Positives = 242/337 (71%), Gaps = 4/337 (1%)

Query: 31  EKLKEGPNLTDFISEDRPKNWDE---YQGKLKREKGESERLRLPPWLKTTIPTGSKFNEI 87
           EKL +GP+  DF++ D+P   DE      K++   G   + RLP WLKT +P G+ FN I
Sbjct: 29  EKLAKGPSFADFLNMDKPLTADEAFELDRKVELPNGSIHK-RLPSWLKTKVPLGTNFNRI 87

Query: 88  KQQLRSLKLSTVCEEARCPNIGECWSGGKHGASTATIMLMGDTCTRGCMFCSVKTSRNPP 147
           K  LR   L TVCEEA+CPNIGECW G     +TATIMLMGDTCTRGC FCSVKTSR P 
Sbjct: 88  KHDLRGSHLHTVCEEAKCPNIGECWGGKDKSRATATIMLMGDTCTRGCRFCSVKTSRRPG 147

Query: 148 PLDPEEPRNTAHAINHWGVGYIVLTSVDRDDLPDGGSLHFAETVKEIKKLNKDILVECLV 207
           PLDP EP NTA AI  W +GYIVLTSVDRDDL D G+ H A+T+++IK+    ILVE L 
Sbjct: 148 PLDPNEPENTAEAIKQWNLGYIVLTSVDRDDLTDLGANHIAKTIQKIKEKAPHILVEALT 207

Query: 208 PDFRAVRGCVARVASSGLDVFAHNVETVERLTPFVRDPRAGYRQTLKVLQMAKEINPDLI 267
           PDF      V  VA SGLDVFAHNVETVE LTPFVRD RA YRQ+L VL+  K+  P LI
Sbjct: 208 PDFSGRMDLVEIVAKSGLDVFAHNVETVEELTPFVRDRRATYRQSLSVLKHVKKTCPHLI 267

Query: 268 TKSSIMLGLGETDAQVEQTMKDLREAGVDCVTLGQYMQPTKKHLKVFEYVTPARFQQWEA 327
           TK+SIMLGLGETDA++  T+KDL E  VD VT GQYM+PTK+HLKV EYV P +F+ W+ 
Sbjct: 268 TKTSIMLGLGETDAEILTTLKDLLEHNVDVVTFGQYMRPTKRHLKVQEYVHPKKFEYWKE 327

Query: 328 RGRQLGFLYVASGALVRSSYRAGEFFISAVLRDRKVN 364
              +LGFLYVASG LVRSSY+AGE+F+  +++ R  N
Sbjct: 328 VAEKLGFLYVASGPLVRSSYKAGEYFMENLIKKRSGN 364


>SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1208

 Score = 31.1 bits (67), Expect = 0.26
 Identities = 18/62 (29%), Positives = 35/62 (56%), Gaps = 3/62 (4%)

Query: 1   MIRNTIKKYKVTKHLDFPRYKHSNKLDAIREKLKEGPNLT---DFISEDRPKNWDEYQGK 57
           +    I+  +VTK L+  R K+SN L+ I +  +E   LT   D I++++ +  +E + +
Sbjct: 494 LAETNIRLQEVTKELETLRMKNSNDLNEIHDLREENEGLTLKIDSITKEKDRLINELEQR 553

Query: 58  LK 59
           +K
Sbjct: 554 IK 555


>SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 450

 Score = 26.2 bits (55), Expect = 7.3
 Identities = 12/27 (44%), Positives = 15/27 (55%)

Query: 46  DRPKNWDEYQGKLKREKGESERLRLPP 72
           D  K  D Y  K + + G +ER RLPP
Sbjct: 412 DAIKAVDSYSQKWQEQTGWTERFRLPP 438


>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 648

 Score = 25.8 bits (54), Expect = 9.7
 Identities = 8/18 (44%), Positives = 15/18 (83%)

Query: 227 VFAHNVETVERLTPFVRD 244
           VFA+ +E ++R+TPF+ +
Sbjct: 394 VFANGIEDIKRITPFLNE 411


>SPBC14C8.11c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 136

 Score = 25.8 bits (54), Expect = 9.7
 Identities = 12/35 (34%), Positives = 18/35 (51%), Gaps = 1/35 (2%)

Query: 154 PRNTAHAINHWGVGYIVLTSVDRDDLPDGGSLHFA 188
           PR+  H  N   + Y+  T+ D   LP+GG+   A
Sbjct: 15  PRSVCHVRNRENI-YLYWTTFDAHKLPNGGNADMA 48


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.319    0.135    0.408 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,654,732
Number of Sequences: 5004
Number of extensions: 67838
Number of successful extensions: 186
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 180
Number of HSP's gapped (non-prelim): 6
length of query: 366
length of database: 2,362,478
effective HSP length: 74
effective length of query: 292
effective length of database: 1,992,182
effective search space: 581717144
effective search space used: 581717144
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 54 (25.8 bits)