Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001084-TA|BGIBMGA001084-PA|IPR003701|DNA repair
exonuclease, IPR004843|Metallophosphoesterase, IPR007281|Mre11,
DNA-binding
         (628 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces pomb...   385   e-107
SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po...    27   5.9  
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc...    27   7.8  
SPCC191.08 |||DUF1715 family protein|Schizosaccharomyces pombe|c...    27   7.8  

>SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 649

 Score =  385 bits (947), Expect = e-107
 Identities = 215/560 (38%), Positives = 314/560 (56%), Gaps = 24/560 (4%)

Query: 12  DDTLRILIASDIHLGFMENDPVRGEDSFIAFEEVLSLAVQCDVDLILLGGDLFDQAKPSV 71
           ++T+RILI+SD H+G+ E DPVRG DSF++F E+L +A + DVD+ILLGGD+F   KPS 
Sbjct: 15  ENTIRILISSDPHVGYGEKDPVRGNDSFVSFNEILEIARERDVDMILLGGDIFHDNKPSR 74

Query: 72  NCMFKCTEIIRKYCLGDKPVSIELLSDQIKNFSRT----VNYEDPNLNISYPILSIHGNH 127
             +++    +R  CLGDKP  +ELLSD       T    +NY DPN+N++ P+ SIHGNH
Sbjct: 75  KALYQALRSLRLNCLGDKPCELELLSDTSLTTGDTAVCNINYLDPNINVAIPVFSIHGNH 134

Query: 128 DDPVGQGSVSSLDILSITGLVNYFGKWTDYTHVRISPVLLQKGLTRLALYGLSHLKDQRL 187
           DDP G G  S+LDIL +TGLVNYFG+  +  ++ +SP+LLQKG T+LALYG+S+++D+RL
Sbjct: 135 DDPSGDGRYSALDILQVTGLVNYFGRVPENDNIVVSPILLQKGFTKLALYGISNVRDERL 194

Query: 188 SRLFAEKKVEMERPDETLD-WFNLFVLHQNHADRGHSNYIPEGVLPNFLDLVVWGHEHDS 246
              F E KV+  RPD   D WFNL  +HQNH+    ++Y+PE  + +F D V+WGHEH+ 
Sbjct: 195 YHSFRENKVKFLRPDLYRDEWFNLLTVHQNHSAHTPTSYLPESFIQDFYDFVLWGHEHE- 253

Query: 247 HICPMKGNKTEKDSFFVVQPGSTVATSLAAGEALPKHCGLLEIHKGNFKLTPLPLQTVRP 306
             C + G+      F VVQPGST+ATSL+ GE  PKHCG+L I   +F L  + L+TVRP
Sbjct: 254 --CLIDGSYNPTQKFTVVQPGSTIATSLSPGETAPKHCGILNITGKDFHLEKIRLRTVRP 311

Query: 307 FIFKTIVLSEENIGSEDVNENEKVQEFLKNRVNEAIDEAS--------KLKTADLRQPLL 358
           FI K I+LSE +     V   ++V  +L ++V EAI EA+         +   +  +P L
Sbjct: 312 FIMKDIILSEVSSIPPMVENKKEVLTYLISKVEEAITEANAQWYEAQGTVPVVENEKPPL 371

Query: 359 PLIRLSIFYERESQNFNRIRFGQNFNGLVANPNDLLIMXXXXXXXXXXXXXXXXXGDMTG 418
           PLIRL + Y    Q  N  RF   F G VAN  D++                     +  
Sbjct: 372 PLIRLRVDYTGGYQTENPQRFSNRFVGRVANATDVVQFYLKKKYTRSKRNDGLYTSAVED 431

Query: 419 VAAEAADVESLLRAYYEAQPKDKRLSVLSVRVITDAVRDFTLKHNEDVLRRAFDAHKRRC 478
           +   +  VESL+  Y     K  RL  L    + +AV +F  K + D ++   +    + 
Sbjct: 432 IKINSLRVESLVNEYL----KTNRLECLPEDSLGEAVVNFVEKDDRDAIKECVETQLNKQ 487

Query: 479 IAALLESTAETEKEIAEQLEVCKRELDEADDEKLHTLVDASARPSAPAAQLI--VPVVK- 535
           I  L++    TE+ + +++     +L +    K     +     S  +  +    PV+K 
Sbjct: 488 INLLVKKRV-TEENLEQEISSIINDLPKISTTKRKDYEELPEEVSETSINIAEHTPVLKH 546

Query: 536 LPNTVGKRTIVVLSSDEEEL 555
             + +   + +  SS E E+
Sbjct: 547 TSSLLDHHSPLATSSSEHEM 566


>SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 432

 Score = 27.5 bits (58), Expect = 5.9
 Identities = 11/24 (45%), Positives = 18/24 (75%)

Query: 326 ENEKVQEFLKNRVNEAIDEASKLK 349
           +NEK +E ++++V E +D A KLK
Sbjct: 48  KNEKSKEIIRSKVIEYLDRAEKLK 71


>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
           Mis17|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 441

 Score = 27.1 bits (57), Expect = 7.8
 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 3/53 (5%)

Query: 314 LSEENIGSEDVNENEKVQEFLKN--RVNEAIDE-ASKLKTADLRQPLLPLIRL 363
           LS+E  GS+ +NE + VQ+F +   + NE ++    K+K A + Q  + L+ L
Sbjct: 264 LSKEAGGSKTLNELDAVQQFFQEFIKENEPLEPYVVKVKNAFVEQVSIRLLEL 316


>SPCC191.08 |||DUF1715 family protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 134

 Score = 27.1 bits (57), Expect = 7.8
 Identities = 11/40 (27%), Positives = 21/40 (52%)

Query: 205 LDWFNLFVLHQNHADRGHSNYIPEGVLPNFLDLVVWGHEH 244
           +DW  +  L +N   RG+   I +G+   + +  ++G EH
Sbjct: 1   MDWEEVTKLEENEYKRGYDEGILKGIEQGYEEAFLFGLEH 40


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.135    0.391 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,685,265
Number of Sequences: 5004
Number of extensions: 111106
Number of successful extensions: 352
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 345
Number of HSP's gapped (non-prelim): 4
length of query: 628
length of database: 2,362,478
effective HSP length: 77
effective length of query: 551
effective length of database: 1,977,170
effective search space: 1089420670
effective search space used: 1089420670
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 57 (27.1 bits)