Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001081-TA|BGIBMGA001081-PA|IPR003307|eIF4-
gamma/eIF5/eIF2-epsilon
         (60 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    34   0.014
At4g18300.1 68417.m02715 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    29   0.29 
At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    28   0.90 
At1g80460.1 68414.m09423 glycerol kinase, putative similar to gl...    26   2.7  

>At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein similar to SP|Q64350
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Rattus norvegicus};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 730

 Score = 33.9 bits (74), Expect = 0.014
 Identities = 15/40 (37%), Positives = 23/40 (57%)

Query: 9   LYNMEVCEEEAFLRWREDVTDAYPGKGEALFQVNTWLTWL 48
           LY+ +V +E+A LRW E+   A       L Q +T++ WL
Sbjct: 671 LYDKDVLQEDAILRWEEEKAGADEADKVYLKQCDTFIQWL 710


>At4g18300.1 68417.m02715 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein similar to SP|Q64350
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Rattus norvegicus};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 709

 Score = 29.5 bits (63), Expect = 0.29
 Identities = 13/41 (31%), Positives = 23/41 (56%)

Query: 8   YLYNMEVCEEEAFLRWREDVTDAYPGKGEALFQVNTWLTWL 48
           +LY+ +V +E+A LRW E+   A       L +  +++ WL
Sbjct: 658 FLYDKDVVQEDAILRWGEEKAGADDCDKVYLNKCESFIKWL 698


>At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein similar to SP|Q13144
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 676

 Score = 27.9 bits (59), Expect = 0.90
 Identities = 13/43 (30%), Positives = 21/43 (48%)

Query: 6   FMYLYNMEVCEEEAFLRWREDVTDAYPGKGEALFQVNTWLTWL 48
           +MY    ++ +E A LRW ++   A       L Q   ++TWL
Sbjct: 625 YMYEEENDLLQEVAILRWSDEKAGADESDKVYLKQCEPFITWL 667


>At1g80460.1 68414.m09423 glycerol kinase, putative similar to
           glycerol kinase (ATP:glycerol 3-phosphotransferase,
           Glycerokinase, GK)[Mycobacterium tuberculosis]
           Swiss-Prot:O69664
          Length = 522

 Score = 26.2 bits (55), Expect = 2.7
 Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)

Query: 25  EDVTDAYPGKGEALF-QVNTWLTW 47
           +DV DA   KG+A+F  ++TWL W
Sbjct: 157 DDVKDAIK-KGDAIFGTIDTWLIW 179


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.327    0.140    0.503 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,345,124
Number of Sequences: 28952
Number of extensions: 34750
Number of successful extensions: 82
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 79
Number of HSP's gapped (non-prelim): 4
length of query: 60
length of database: 12,070,560
effective HSP length: 40
effective length of query: 20
effective length of database: 10,912,480
effective search space: 218249600
effective search space used: 218249600
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.7 bits)
S2: 51 (24.6 bits)