Silkworm BLAST Result against nematostella

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001077-TA|BGIBMGA001077-PA|IPR001159|Double-stranded RNA
binding
         (320 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_50149| Best HMM Match : No HMM Matches (HMM E-Value=.)              54   2e-07
SB_474| Best HMM Match : dsrm (HMM E-Value=3.2e-20)                    46   4e-05
SB_3066| Best HMM Match : dsrm (HMM E-Value=2.2e-32)                   40   0.002
SB_4602| Best HMM Match : dsrm (HMM E-Value=5.9e-14)                   40   0.003
SB_27558| Best HMM Match : dsrm (HMM E-Value=9.6e-18)                  38   0.008
SB_1180| Best HMM Match : DDE (HMM E-Value=5e-08)                      31   1.7  
SB_3213| Best HMM Match : zf-C2H2 (HMM E-Value=3.8e-40)                29   5.1  
SB_28694| Best HMM Match : RVT_1 (HMM E-Value=4.6e-28)                 29   5.1  
SB_46816| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   6.7  
SB_10751| Best HMM Match : TNFR_c6 (HMM E-Value=7.6e-17)               28   8.8  

>SB_50149| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 656

 Score = 54.0 bits (124), Expect = 2e-07
 Identities = 37/167 (22%), Positives = 76/167 (45%), Gaps = 4/167 (2%)

Query: 2   KTPITVLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSASARSKREAKQEAA 61
           K+ I+ + E+  ++  + +++ +  +GP H   F+ +              K++AK+EAA
Sbjct: 237 KSEISQVYEIATRMKMMVDFQDMENAGPAHMKKFKIKATVGPYMTEGEGNRKKDAKKEAA 296

Query: 62  RAMLLCLSTIGH-RVPPPFATEFTQPSHSNQSAGECSEGKAPTVDSRSYVALLKELCEEY 120
             ML  L  +    V P    ++ +    N    +      PT++S   +++L ++ +  
Sbjct: 297 VKMLAELKKLPEPPVDPEKKKKYQRHGLYNAKKVKPKSDIDPTLNS---ISILGQILQRR 353

Query: 121 KLPGVEYALVADTGPAHMRLFSVRASIGLHSRDASGTTKRQARQKAA 167
             P   Y+LV + G    + F++R  +G H     G  K+ A++ AA
Sbjct: 354 HDPAPVYSLVEERGHMMKKEFTLRVKVGSHEALGVGPNKKTAKKNAA 400



 Score = 43.2 bits (97), Expect = 3e-04
 Identities = 27/120 (22%), Positives = 58/120 (48%), Gaps = 2/120 (1%)

Query: 51  RSKREAKQEAARAMLLCLSTIGHRVPPPFATEFTQPSHSNQSAGECSEGKAPTVDSRSYV 110
           R K++A+ +AA+  +  L             E +  ++   S G    G       +S +
Sbjct: 182 RDKQKARHDAAQKAMKVLREELLTQMSQCGIEVSGDTNETVSNG-VDAGDEEDESDKSEI 240

Query: 111 ALLKELCEEYKLPGVEYALVADTGPAHMRLFSVRASIGLHSRDASGTTKRQARQKAAADL 170
           + + E+    K+  V++  + + GPAHM+ F ++A++G +  +  G  K+ A+++AA  +
Sbjct: 241 SQVYEIATRMKMM-VDFQDMENAGPAHMKKFKIKATVGPYMTEGEGNRKKDAKKEAAVKM 299



 Score = 36.7 bits (81), Expect = 0.025
 Identities = 16/43 (37%), Positives = 27/43 (62%), Gaps = 1/43 (2%)

Query: 2  KTPITVLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGE 44
          KTPI+++ E+       PEY  + ++GP H+ TF+ + K LG+
Sbjct: 4  KTPISLMNELAKANNLNPEYNVIEETGPAHKKTFKVQLK-LGD 45



 Score = 33.5 bits (73), Expect = 0.23
 Identities = 18/65 (27%), Positives = 35/65 (53%), Gaps = 2/65 (3%)

Query: 104 VDSRSYVALLKELCEEYKLPGVEYALVADTGPAHMRLFSVRASIG-LHSRDASGTTKRQA 162
           +  ++ ++L+ EL +   L   EY ++ +TGPAH + F V+  +G +   +      + A
Sbjct: 1   MSQKTPISLMNELAKANNL-NPEYNVIEETGPAHKKTFKVQLKLGDIGIWEGMANNIKLA 59

Query: 163 RQKAA 167
           + KAA
Sbjct: 60  KHKAA 64


>SB_474| Best HMM Match : dsrm (HMM E-Value=3.2e-20)
          Length = 918

 Score = 46.0 bits (104), Expect = 4e-05
 Identities = 25/64 (39%), Positives = 37/64 (57%)

Query: 2  KTPITVLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSASARSKREAKQEAA 61
          KTPI+ L E+ +++G   ++E V+  G  H  TF   CKA   +   +  SK++AKQ AA
Sbjct: 33 KTPISELLELAVQMGIKVDFEQVSAVGMPHNQTFTINCKAGDFTGIGTGPSKKKAKQNAA 92

Query: 62 RAML 65
           A L
Sbjct: 93 SATL 96



 Score = 30.3 bits (65), Expect = 2.2
 Identities = 15/30 (50%), Positives = 18/30 (60%), Gaps = 1/30 (3%)

Query: 8   LQEMMMKLG-QIPEYECVAQSGPQHQATFE 36
           LQE+ +K G Q PEY+ V   GP H   FE
Sbjct: 128 LQEVTVKKGWQYPEYKDVKAKGPPHMRQFE 157


>SB_3066| Best HMM Match : dsrm (HMM E-Value=2.2e-32)
          Length = 429

 Score = 40.3 bits (90), Expect = 0.002
 Identities = 23/71 (32%), Positives = 39/71 (54%), Gaps = 2/71 (2%)

Query: 97  SEGKAPTVDSRSYVALLKELCEEYKLPGVEYALVADTGPAHMRLFSVRASIGLHSRDASG 156
           S  K  T  + ++   L +L  EYK PG+E+ LV+  GP H   F V  ++   + +  G
Sbjct: 44  SAKKRKTSSNATFKTPLMQL-NEYK-PGLEFRLVSQEGPPHDTTFVVAVTVNGQTYEGKG 101

Query: 157 TTKRQARQKAA 167
           ++K++A+  AA
Sbjct: 102 SSKQRAKHDAA 112



 Score = 39.1 bits (87), Expect = 0.005
 Identities = 32/107 (29%), Positives = 44/107 (41%), Gaps = 8/107 (7%)

Query: 2   KTPITVLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSASARSKREAKQEAA 61
           KTP+  L E    L    E+  V+Q GP H  TF       G++      SK+ AK +AA
Sbjct: 57  KTPLMQLNEYKPGL----EFRLVSQEGPPHDTTFVVAVTVNGQTYEGKGSSKQRAKHDAA 112

Query: 62  RAMLLCLSTIGHRVPPPFA---TEFTQPSHSNQSAGECSEGKAPTVD 105
              L     +   +   +A    +FT  +  N S  E SE    T D
Sbjct: 113 EKALQSCVQLPFNIKTEYAPTNEDFTADATGN-SFTENSENGQKTSD 158


>SB_4602| Best HMM Match : dsrm (HMM E-Value=5.9e-14)
          Length = 579

 Score = 39.9 bits (89), Expect = 0.003
 Identities = 20/66 (30%), Positives = 33/66 (50%)

Query: 124 GVEYALVADTGPAHMRLFSVRASIGLHSRDASGTTKRQARQKAAADLYLNKHERAMERLV 183
           G+EY ++A  GP+H   +S R  +G  S DA+    ++  ++ AADL L    +      
Sbjct: 394 GIEYEMLAPAGPSHSPQYSARVRVGESSFDAATAATKKDAKEFAADLALRALSQEPGTFA 453

Query: 184 ETTPAP 189
             +P P
Sbjct: 454 MYSPIP 459



 Score = 35.5 bits (78), Expect = 0.058
 Identities = 21/52 (40%), Positives = 32/52 (61%), Gaps = 3/52 (5%)

Query: 20  EYECVAQSGPQHQATFEFRCKALGESV--SASARSKREAKQEAARAMLLCLS 69
           EYE +A +GP H   +  R + +GES   +A+A +K++AK+ AA   L  LS
Sbjct: 396 EYEMLAPAGPSHSPQYSARVR-VGESSFDAATAATKKDAKEFAADLALRALS 446


>SB_27558| Best HMM Match : dsrm (HMM E-Value=9.6e-18)
          Length = 765

 Score = 38.3 bits (85), Expect = 0.008
 Identities = 23/72 (31%), Positives = 34/72 (47%), Gaps = 2/72 (2%)

Query: 2   KTPITVLQEMMM-KLGQIPEYECVAQSGPQHQATFEFRCKALGESVSASARSKREAKQEA 60
           K PI++LQE+   K  Q+P+++ +   GP H   F F+    G        S  + KQ  
Sbjct: 687 KHPISMLQEICARKKWQLPDFQLIFDHGPPHSKQFLFKVAVNGYEYQPQVVSSNK-KQAK 745

Query: 61  ARAMLLCLSTIG 72
           A+  L  L  IG
Sbjct: 746 AQCALYALKAIG 757



 Score = 36.3 bits (80), Expect = 0.033
 Identities = 20/90 (22%), Positives = 38/90 (42%), Gaps = 2/90 (2%)

Query: 87  SHSNQSAGECSEGKAPTVD--SRSYVALLKELCEEYKLPGVEYALVADTGPAHMRLFSVR 144
           S   +  G    G  P  D   +  +++L+E+C   K    ++ L+ D GP H + F  +
Sbjct: 665 SSGGEKPGNKKPGNRPITDLQGKHPISMLQEICARKKWQLPDFQLIFDHGPPHSKQFLFK 724

Query: 145 ASIGLHSRDASGTTKRQARQKAAADLYLNK 174
            ++  +       +  + + KA   LY  K
Sbjct: 725 VAVNGYEYQPQVVSSNKKQAKAQCALYALK 754


>SB_1180| Best HMM Match : DDE (HMM E-Value=5e-08)
          Length = 265

 Score = 30.7 bits (66), Expect = 1.7
 Identities = 14/42 (33%), Positives = 23/42 (54%)

Query: 104 VDSRSYVALLKELCEEYKLPGVEYALVADTGPAHMRLFSVRA 145
           +DS  +   ++E    ++ PG   ALV D GPAH  + + +A
Sbjct: 203 LDSNLFEEWVREQDRSFERPGRNVALVVDNGPAHPTVSNFKA 244


>SB_3213| Best HMM Match : zf-C2H2 (HMM E-Value=3.8e-40)
          Length = 595

 Score = 29.1 bits (62), Expect = 5.1
 Identities = 18/84 (21%), Positives = 35/84 (41%), Gaps = 2/84 (2%)

Query: 135 PAHMRLFSVRASIGLHSRDASGTTKRQARQKAAADLYLNKHERAMERLVETTPAPWKPDL 194
           P  ++LF     +  H    +G  +R  + ++ +  + N       R + T   P+K ++
Sbjct: 494 PYCLKLFRYSGDLKQHINIHTG--QRPYQCESCSKAFTNHSTLRQHRRIHTGERPYKCEI 551

Query: 195 GQRVSEYHYGLISHTGECTARSRR 218
             R   YH  L  H    T+ +R+
Sbjct: 552 CDRTYRYHSSLKQHLATHTSMARK 575


>SB_28694| Best HMM Match : RVT_1 (HMM E-Value=4.6e-28)
          Length = 1045

 Score = 29.1 bits (62), Expect = 5.1
 Identities = 24/71 (33%), Positives = 31/71 (43%), Gaps = 4/71 (5%)

Query: 103  TVDSRSYVALLKELCEEYKLPGVEYALVADTGPAHMRL-FSVRASIGLHSRDASGTTKRQ 161
            TV SR   A++KEL   +   G+   L+ D GP      FSV A   +     S     Q
Sbjct: 968  TVTSR---AIIKELKAIFARFGIPDTLITDNGPQFASAEFSVFAKTWMFEHKTSSPRYAQ 1024

Query: 162  ARQKAAADLYL 172
            A  KA   +YL
Sbjct: 1025 ANGKAENAVYL 1035


>SB_46816| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1165

 Score = 28.7 bits (61), Expect = 6.7
 Identities = 13/48 (27%), Positives = 26/48 (54%), Gaps = 1/48 (2%)

Query: 91  QSAGECSEGKAPTVDSRSYVALLKELCEEYKLPGVEYALVADTGPAHM 138
           Q A +C +     + S +  A+ + +CE + L G+   +V+D GP ++
Sbjct: 890 QEAQDC-DAICKQLGSTTSAAVTRHMCEIFALHGIPDTVVSDNGPQYV 936


>SB_10751| Best HMM Match : TNFR_c6 (HMM E-Value=7.6e-17)
          Length = 738

 Score = 28.3 bits (60), Expect = 8.8
 Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 7/82 (8%)

Query: 71  IGHRVPPPFATEFTQPSHSNQSAGECSEGK--APTVDSRSYVALLK---ELCEEYKLPGV 125
           +GH + PP  +  T P  +N S  +C+ GK  + ++DS S     K       E      
Sbjct: 190 VGHGLSPPCGSVLTNP--ANHSCVKCAPGKSFSESIDSSSCKPCSKCAVGQMVERACNST 247

Query: 126 EYALVADTGPAHMRLFSVRASI 147
              L  D  P   R FSV  ++
Sbjct: 248 HDTLCGDCSPGFYRDFSVHPAV 269


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.317    0.129    0.382 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,711,089
Number of Sequences: 59808
Number of extensions: 352696
Number of successful extensions: 937
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 922
Number of HSP's gapped (non-prelim): 18
length of query: 320
length of database: 16,821,457
effective HSP length: 82
effective length of query: 238
effective length of database: 11,917,201
effective search space: 2836293838
effective search space used: 2836293838
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 60 (28.3 bits)