BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001077-TA|BGIBMGA001077-PA|IPR001159|Double-stranded RNA binding (320 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g62800.2 68416.m07056 double-stranded RNA-binding domain (DsR... 33 0.34 At3g62800.1 68416.m07055 double-stranded RNA-binding domain (DsR... 33 0.34 At3g26932.1 68416.m03370 double-stranded RNA-binding domain (DsR... 32 0.44 At1g19270.1 68414.m02397 ubiquitin interaction motif-containing ... 32 0.59 At4g01290.1 68417.m00170 expressed protein 30 1.8 At1g80650.1 68414.m09464 double-stranded RNA-binding domain (DsR... 29 3.1 At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /... 29 3.1 At3g59830.1 68416.m06676 ankyrin protein kinase, putative simila... 29 5.5 At1g53370.1 68414.m06050 F-box family protein contains F-box dom... 28 9.6 At1g33430.1 68414.m04138 galactosyltransferase family protein co... 28 9.6 >At3g62800.2 68416.m07056 double-stranded RNA-binding domain (DsRBD)-containing protein weak similarity to SP|P19525 Interferon-induced, double-stranded RNA-activated protein kinase (EC 2.7.1.-) {Homo sapiens}; contains Pfam profile PF00035: Double-stranded RNA binding motif Length = 355 Score = 32.7 bits (71), Expect = 0.34 Identities = 18/63 (28%), Positives = 31/63 (49%), Gaps = 1/63 (1%) Query: 7 VLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSA-SARSKREAKQEAARAML 65 +LQE+ K + + A SGP H TF + G+ S A++K+ A+ AA+ Sbjct: 86 LLQEIAQKESSLLPFYATATSGPSHAPTFTSTVEFAGKVFSGEEAKTKKLAEMSAAKVAF 145 Query: 66 LCL 68 + + Sbjct: 146 MSI 148 >At3g62800.1 68416.m07055 double-stranded RNA-binding domain (DsRBD)-containing protein weak similarity to SP|P19525 Interferon-induced, double-stranded RNA-activated protein kinase (EC 2.7.1.-) {Homo sapiens}; contains Pfam profile PF00035: Double-stranded RNA binding motif Length = 355 Score = 32.7 bits (71), Expect = 0.34 Identities = 18/63 (28%), Positives = 31/63 (49%), Gaps = 1/63 (1%) Query: 7 VLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSA-SARSKREAKQEAARAML 65 +LQE+ K + + A SGP H TF + G+ S A++K+ A+ AA+ Sbjct: 86 LLQEIAQKESSLLPFYATATSGPSHAPTFTSTVEFAGKVFSGEEAKTKKLAEMSAAKVAF 145 Query: 66 LCL 68 + + Sbjct: 146 MSI 148 >At3g26932.1 68416.m03370 double-stranded RNA-binding domain (DsRBD)-containing protein contains Pfam profile PF00035: Double-stranded RNA binding motif Length = 301 Score = 32.3 bits (70), Expect = 0.44 Identities = 22/59 (37%), Positives = 34/59 (57%), Gaps = 3/59 (5%) Query: 7 VLQEMMMKLG-QIPEYECVAQSGPQHQATFEFRCKALGESVSA-SARSKREAKQEAARA 63 +LQE + G +P Y V +SGP H TF + G S + SA++K++A++ AA A Sbjct: 33 LLQETAHRAGLDLPVYTSV-RSGPGHIPTFSCTVELAGMSFNGESAKTKKQAEKNAAIA 90 >At1g19270.1 68414.m02397 ubiquitin interaction motif-containing protein / LIM domain-containing protein weak similarity to LIM-homeobox protein [Mus musculus] GI:2149584, Hic-5 [Mus musculus] GI:664955; contains Pfam profiles PF02809: Ubiquitin interaction motif, PF00412: LIM domain Length = 532 Score = 31.9 bits (69), Expect = 0.59 Identities = 24/91 (26%), Positives = 38/91 (41%), Gaps = 3/91 (3%) Query: 85 QPSHSNQSAGECSEGKAPTVDSRSYVALLKELCEEYKLPGVEYALVADTGPAHMRLFSVR 144 +PS S + E + +D ++LL+E E+ + G +Y++ D R Sbjct: 57 EPSTSEDNTSNDQENE--DIDRAIALSLLEENQEQTSISG-KYSMPVDEDEQLARALQES 113 Query: 145 ASIGLHSRDASGTTKRQARQKAAADLYLNKH 175 +G R SG+T A DLY N H Sbjct: 114 MVVGNSPRHKSGSTYDNGNAYGAGDLYGNGH 144 >At4g01290.1 68417.m00170 expressed protein Length = 991 Score = 30.3 bits (65), Expect = 1.8 Identities = 23/73 (31%), Positives = 34/73 (46%), Gaps = 2/73 (2%) Query: 68 LSTIGHRVPPP-FATEFTQPSHSNQSAGECSEGKAPTVDSRSYVALLKELCEEYKLPGVE 126 LS R PPP T PS + +AGE GK+ T+++ A + EL + P Sbjct: 630 LSATERRPPPPSMKTTTPPPSVKSTTAGEADPGKSLTLENLFGSAFMNEL-QSIGEPVSG 688 Query: 127 YALVADTGPAHMR 139 A+V+D +R Sbjct: 689 RAMVSDAPGVPLR 701 >At1g80650.1 68414.m09464 double-stranded RNA-binding domain (DsRBD)-containing protein contains Pfam profile PF00035: Double-stranded RNA binding motif Length = 198 Score = 29.5 bits (63), Expect = 3.1 Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 2/48 (4%) Query: 111 ALLKELCEEYKLPGVEYALVADTGPAHMRLFSVRASIGLHSRDASGTT 158 ++L E+C + Y GP H+RLF+ + + + RD+SG T Sbjct: 117 SVLHEMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEI--RDSSGKT 162 >At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory / CAF identical to RNA helicase/RNAseIII CAF protein GB:AAF03534 GI:6102610 from [Arabidopsis thaliana] Length = 1909 Score = 29.5 bits (63), Expect = 3.1 Identities = 31/149 (20%), Positives = 55/149 (36%), Gaps = 7/149 (4%) Query: 4 PITVLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSASARSKREAKQEAARA 63 P+ LQE + + EY+ ++SG + AT E + V+ + + K K A A Sbjct: 1734 PVRELQERCQQQAEGLEYKA-SRSG--NTATVEVFIDGVQVGVAQNPQKKMAQKLAARNA 1790 Query: 64 MLLCLSTIGHRVPPPFATEFTQPSHSNQSAGECSEGKAPTVDSRSYVALLKELCEEYKLP 123 L+ + + + ++ + GE G L ++C P Sbjct: 1791 ----LAALKEKEIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWP 1846 Query: 124 GVEYALVADTGPAHMRLFSVRASIGLHSR 152 Y V + GPAH + F+ + R Sbjct: 1847 MPSYRCVKEGGPAHAKRFTFGVRVNTSDR 1875 >At3g59830.1 68416.m06676 ankyrin protein kinase, putative similar to ankyrin-kinase [Medicago truncatula] gi|18700701|gb|AAL78674 Length = 477 Score = 28.7 bits (61), Expect = 5.5 Identities = 15/26 (57%), Positives = 17/26 (65%), Gaps = 2/26 (7%) Query: 96 CSEGKAPTV--DSRSYVALLKELCEE 119 C EGK PT+ S+SY LKEL EE Sbjct: 411 CIEGKRPTIRTKSKSYPPELKELIEE 436 >At1g53370.1 68414.m06050 F-box family protein contains F-box domain Pfam:PF00646 Length = 355 Score = 27.9 bits (59), Expect = 9.6 Identities = 12/52 (23%), Positives = 26/52 (50%), Gaps = 2/52 (3%) Query: 109 YVALLKELCEEYKLPGVEYALVADTGPAHMRLFSVRASIGLHSRDASGTTKR 160 Y K +C ++K+ V + + G ++RLF+ + +G+H D ++ Sbjct: 215 YQGSSKIVCFDFKIEKVSFVNI--DGMCNLRLFNCKGKLGVHQYDVQSRNEK 264 >At1g33430.1 68414.m04138 galactosyltransferase family protein contains Pfam profile: PF01762 galactosyltransferase Length = 395 Score = 27.9 bits (59), Expect = 9.6 Identities = 15/47 (31%), Positives = 24/47 (51%) Query: 8 LQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSASARSKR 54 + E M G P+ + AQ+G A+F++ C + +SV AR R Sbjct: 333 VDERSMCCGTPPDCQWKAQAGNVCAASFDWSCSGICKSVDRMARVHR 379 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.317 0.129 0.382 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,651,876 Number of Sequences: 28952 Number of extensions: 237585 Number of successful extensions: 562 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 558 Number of HSP's gapped (non-prelim): 13 length of query: 320 length of database: 12,070,560 effective HSP length: 81 effective length of query: 239 effective length of database: 9,725,448 effective search space: 2324382072 effective search space used: 2324382072 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 59 (27.9 bits)
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