BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001077-TA|BGIBMGA001077-PA|IPR001159|Double-stranded RNA
binding
(320 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g62800.2 68416.m07056 double-stranded RNA-binding domain (DsR... 33 0.34
At3g62800.1 68416.m07055 double-stranded RNA-binding domain (DsR... 33 0.34
At3g26932.1 68416.m03370 double-stranded RNA-binding domain (DsR... 32 0.44
At1g19270.1 68414.m02397 ubiquitin interaction motif-containing ... 32 0.59
At4g01290.1 68417.m00170 expressed protein 30 1.8
At1g80650.1 68414.m09464 double-stranded RNA-binding domain (DsR... 29 3.1
At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /... 29 3.1
At3g59830.1 68416.m06676 ankyrin protein kinase, putative simila... 29 5.5
At1g53370.1 68414.m06050 F-box family protein contains F-box dom... 28 9.6
At1g33430.1 68414.m04138 galactosyltransferase family protein co... 28 9.6
>At3g62800.2 68416.m07056 double-stranded RNA-binding domain
(DsRBD)-containing protein weak similarity to SP|P19525
Interferon-induced, double-stranded RNA-activated
protein kinase (EC 2.7.1.-) {Homo sapiens}; contains
Pfam profile PF00035: Double-stranded RNA binding motif
Length = 355
Score = 32.7 bits (71), Expect = 0.34
Identities = 18/63 (28%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
Query: 7 VLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSA-SARSKREAKQEAARAML 65
+LQE+ K + + A SGP H TF + G+ S A++K+ A+ AA+
Sbjct: 86 LLQEIAQKESSLLPFYATATSGPSHAPTFTSTVEFAGKVFSGEEAKTKKLAEMSAAKVAF 145
Query: 66 LCL 68
+ +
Sbjct: 146 MSI 148
>At3g62800.1 68416.m07055 double-stranded RNA-binding domain
(DsRBD)-containing protein weak similarity to SP|P19525
Interferon-induced, double-stranded RNA-activated
protein kinase (EC 2.7.1.-) {Homo sapiens}; contains
Pfam profile PF00035: Double-stranded RNA binding motif
Length = 355
Score = 32.7 bits (71), Expect = 0.34
Identities = 18/63 (28%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
Query: 7 VLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSA-SARSKREAKQEAARAML 65
+LQE+ K + + A SGP H TF + G+ S A++K+ A+ AA+
Sbjct: 86 LLQEIAQKESSLLPFYATATSGPSHAPTFTSTVEFAGKVFSGEEAKTKKLAEMSAAKVAF 145
Query: 66 LCL 68
+ +
Sbjct: 146 MSI 148
>At3g26932.1 68416.m03370 double-stranded RNA-binding domain
(DsRBD)-containing protein contains Pfam profile
PF00035: Double-stranded RNA binding motif
Length = 301
Score = 32.3 bits (70), Expect = 0.44
Identities = 22/59 (37%), Positives = 34/59 (57%), Gaps = 3/59 (5%)
Query: 7 VLQEMMMKLG-QIPEYECVAQSGPQHQATFEFRCKALGESVSA-SARSKREAKQEAARA 63
+LQE + G +P Y V +SGP H TF + G S + SA++K++A++ AA A
Sbjct: 33 LLQETAHRAGLDLPVYTSV-RSGPGHIPTFSCTVELAGMSFNGESAKTKKQAEKNAAIA 90
>At1g19270.1 68414.m02397 ubiquitin interaction motif-containing
protein / LIM domain-containing protein weak similarity
to LIM-homeobox protein [Mus musculus] GI:2149584, Hic-5
[Mus musculus] GI:664955; contains Pfam profiles
PF02809: Ubiquitin interaction motif, PF00412: LIM
domain
Length = 532
Score = 31.9 bits (69), Expect = 0.59
Identities = 24/91 (26%), Positives = 38/91 (41%), Gaps = 3/91 (3%)
Query: 85 QPSHSNQSAGECSEGKAPTVDSRSYVALLKELCEEYKLPGVEYALVADTGPAHMRLFSVR 144
+PS S + E + +D ++LL+E E+ + G +Y++ D R
Sbjct: 57 EPSTSEDNTSNDQENE--DIDRAIALSLLEENQEQTSISG-KYSMPVDEDEQLARALQES 113
Query: 145 ASIGLHSRDASGTTKRQARQKAAADLYLNKH 175
+G R SG+T A DLY N H
Sbjct: 114 MVVGNSPRHKSGSTYDNGNAYGAGDLYGNGH 144
>At4g01290.1 68417.m00170 expressed protein
Length = 991
Score = 30.3 bits (65), Expect = 1.8
Identities = 23/73 (31%), Positives = 34/73 (46%), Gaps = 2/73 (2%)
Query: 68 LSTIGHRVPPP-FATEFTQPSHSNQSAGECSEGKAPTVDSRSYVALLKELCEEYKLPGVE 126
LS R PPP T PS + +AGE GK+ T+++ A + EL + P
Sbjct: 630 LSATERRPPPPSMKTTTPPPSVKSTTAGEADPGKSLTLENLFGSAFMNEL-QSIGEPVSG 688
Query: 127 YALVADTGPAHMR 139
A+V+D +R
Sbjct: 689 RAMVSDAPGVPLR 701
>At1g80650.1 68414.m09464 double-stranded RNA-binding domain
(DsRBD)-containing protein contains Pfam profile
PF00035: Double-stranded RNA binding motif
Length = 198
Score = 29.5 bits (63), Expect = 3.1
Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
Query: 111 ALLKELCEEYKLPGVEYALVADTGPAHMRLFSVRASIGLHSRDASGTT 158
++L E+C + Y GP H+RLF+ + + + RD+SG T
Sbjct: 117 SVLHEMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEI--RDSSGKT 162
>At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory / CAF
identical to RNA helicase/RNAseIII CAF protein
GB:AAF03534 GI:6102610 from [Arabidopsis thaliana]
Length = 1909
Score = 29.5 bits (63), Expect = 3.1
Identities = 31/149 (20%), Positives = 55/149 (36%), Gaps = 7/149 (4%)
Query: 4 PITVLQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSASARSKREAKQEAARA 63
P+ LQE + + EY+ ++SG + AT E + V+ + + K K A A
Sbjct: 1734 PVRELQERCQQQAEGLEYKA-SRSG--NTATVEVFIDGVQVGVAQNPQKKMAQKLAARNA 1790
Query: 64 MLLCLSTIGHRVPPPFATEFTQPSHSNQSAGECSEGKAPTVDSRSYVALLKELCEEYKLP 123
L+ + + + ++ + GE G L ++C P
Sbjct: 1791 ----LAALKEKEIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWP 1846
Query: 124 GVEYALVADTGPAHMRLFSVRASIGLHSR 152
Y V + GPAH + F+ + R
Sbjct: 1847 MPSYRCVKEGGPAHAKRFTFGVRVNTSDR 1875
>At3g59830.1 68416.m06676 ankyrin protein kinase, putative similar
to ankyrin-kinase [Medicago truncatula]
gi|18700701|gb|AAL78674
Length = 477
Score = 28.7 bits (61), Expect = 5.5
Identities = 15/26 (57%), Positives = 17/26 (65%), Gaps = 2/26 (7%)
Query: 96 CSEGKAPTV--DSRSYVALLKELCEE 119
C EGK PT+ S+SY LKEL EE
Sbjct: 411 CIEGKRPTIRTKSKSYPPELKELIEE 436
>At1g53370.1 68414.m06050 F-box family protein contains F-box domain
Pfam:PF00646
Length = 355
Score = 27.9 bits (59), Expect = 9.6
Identities = 12/52 (23%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
Query: 109 YVALLKELCEEYKLPGVEYALVADTGPAHMRLFSVRASIGLHSRDASGTTKR 160
Y K +C ++K+ V + + G ++RLF+ + +G+H D ++
Sbjct: 215 YQGSSKIVCFDFKIEKVSFVNI--DGMCNLRLFNCKGKLGVHQYDVQSRNEK 264
>At1g33430.1 68414.m04138 galactosyltransferase family protein
contains Pfam profile: PF01762 galactosyltransferase
Length = 395
Score = 27.9 bits (59), Expect = 9.6
Identities = 15/47 (31%), Positives = 24/47 (51%)
Query: 8 LQEMMMKLGQIPEYECVAQSGPQHQATFEFRCKALGESVSASARSKR 54
+ E M G P+ + AQ+G A+F++ C + +SV AR R
Sbjct: 333 VDERSMCCGTPPDCQWKAQAGNVCAASFDWSCSGICKSVDRMARVHR 379
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.129 0.382
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,651,876
Number of Sequences: 28952
Number of extensions: 237585
Number of successful extensions: 562
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 558
Number of HSP's gapped (non-prelim): 13
length of query: 320
length of database: 12,070,560
effective HSP length: 81
effective length of query: 239
effective length of database: 9,725,448
effective search space: 2324382072
effective search space used: 2324382072
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 59 (27.9 bits)
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