Silkworm BLAST Result against nematostella

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001061-TA|BGIBMGA001061-PA|IPR005123|2OG-Fe(II)
oxygenase, IPR013216|Methyltransferase type 11
         (594 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_34115| Best HMM Match : F5_F8_type_C (HMM E-Value=0)               194   1e-49
SB_55144| Best HMM Match : CMAS (HMM E-Value=0.72)                     99   9e-21
SB_57673| Best HMM Match : No HMM Matches (HMM E-Value=.)              49   9e-06
SB_58733| Best HMM Match : No HMM Matches (HMM E-Value=.)              44   3e-04
SB_49517| Best HMM Match : Hydrolase (HMM E-Value=2.7e-27)             30   6.1  

>SB_34115| Best HMM Match : F5_F8_type_C (HMM E-Value=0)
          Length = 1572

 Score =  194 bits (474), Expect = 1e-49
 Identities = 118/320 (36%), Positives = 173/320 (54%), Gaps = 24/320 (7%)

Query: 29  DPRPNVILCNVGQATGFDKQKLLKLLTEYKVNTPLPKFVAEKGESYSYLMFDKAENASLF 88
           +P P++++ N G   G D++ + ++ + Y     L + V     SYS++ +    NA   
Sbjct: 45  EPTPHILVGNGGLMCGVDRESITRVFSLYGT---LERVVMLPQRSYSFVSYKSHNNALNA 101

Query: 89  YEACNGKAQVDENG--TTLYVTFVENVPDTDIICKHPNPKGLHIIPDFLTEDEEKLFLNT 146
               NGKA +DE G  +  Y++++  VP   I+ K  +P GL I  +F+ E+EEK  L+ 
Sbjct: 102 MRNLNGKA-LDEFGLRSVFYLSYLVKVPSDVILSKIEHPPGLQIYEEFINEEEEKTLLDA 160

Query: 147 FNIQHVETT-----------LKNRQVKHYGYEFRYGSNDVDLSCPLQEKIPKICKLLWRR 195
                 E             L++R+VKHYGYEF YG+ND+D + PL   +P +C  +  R
Sbjct: 161 LGWDGTEDQSRSTDAAPQKELRHRRVKHYGYEFLYGTNDIDRAKPLPGGMPAVCNDILTR 220

Query: 196 LQHYGYDLGVPVQLTVNKYLPGQGIPSHVDKHSPFGETILALSLGSNVVMDWKHHTGKYV 255
           +   G     P QLTVN+YLPGQGIP HVD HS F + I +LSLG+ + MD++H   ++V
Sbjct: 221 MVSQGAVQNTPDQLTVNEYLPGQGIPPHVDTHSAFEDGICSLSLGAKISMDFRHPDSRHV 280

Query: 256 PVMVEARSMMIMQDEARYDWQHG---IQPRMWDPVL--DVRLIDNEKVKVITSDTVQ--R 308
            V++  RS+++M  E+RY W HG   I     D  +  D   I  +   V TS   Q  R
Sbjct: 281 SVLLPRRSLLVMSGESRYLWTHGKFDIIGSGLDTSIHEDQESIAADASNVSTSGVTQYER 340

Query: 309 EMRISLTFRWTRCGPCKCEY 328
           E RISLTFR     PC C+Y
Sbjct: 341 ERRISLTFRKILHVPCTCKY 360



 Score = 62.1 bits (144), Expect = 1e-09
 Identities = 30/70 (42%), Positives = 43/70 (61%), Gaps = 2/70 (2%)

Query: 519 HLPVHENRTQFQHKDLLVPWNLRSKAPPLQQ-PDTTFLRYYHVFDEGELDQLCRDARLAI 577
           H+ V+ +R +F+ +DL VPW  R  +       DT F R+YHVF EGEL +LCR     +
Sbjct: 480 HVEVNVSRGEFKQQDLFVPWKFRGASKKESNGADTVFHRFYHVFKEGELLELCRCLDNVV 539

Query: 578 VR-SFYEEGN 586
           V+ S+Y+ GN
Sbjct: 540 VKDSYYDRGN 549



 Score = 51.6 bits (118), Expect = 2e-06
 Identities = 21/49 (42%), Positives = 33/49 (67%)

Query: 446 KDECADFIICVAVVHHFSTQARRLHSLQTIHRLLRTNGQALITVWAKDQ 494
           K+   D  +C+AV+HH ST  RR+ +L+ + R++R  G AL+ VWA +Q
Sbjct: 363 KNNAFDVCLCIAVIHHLSTTERRIAALRELVRIIRPAGLALVYVWALEQ 411


>SB_55144| Best HMM Match : CMAS (HMM E-Value=0.72)
          Length = 529

 Score = 99.1 bits (236), Expect = 9e-21
 Identities = 48/145 (33%), Positives = 78/145 (53%), Gaps = 5/145 (3%)

Query: 350 NIEDIHVHQVYEQIAGHFSTTRHKPWPKVVDFMQHVPAGSIVLDLGCGNGKNILNRTDIL 409
           ++E   VH+ YE+IA  F   R+K WP V  F++  P GS++ D+GCG GK +   TD  
Sbjct: 12  DLEKRFVHETYEEIAPGFRNARYKAWPCVTQFIKAQPKGSVIADIGCGTGKYLSISTDAF 71

Query: 410 QVAGERSSGLLEECKGLTARISGADCIRLDLLNTGLKDECADFIICVAVVHHFSTQARRL 469
               +     +E  +     +S  D + L       +D+C D +I V V+HH ++  RRL
Sbjct: 72  ITGSDCCPKFVEIARERQHEVSLCDNLSLP-----YRDDCLDAVISVGVIHHLASSKRRL 126

Query: 470 HSLQTIHRLLRTNGQALITVWAKDQ 494
            ++  + R+LR  G+ ++ VWA +Q
Sbjct: 127 QAICELARVLRPGGKMMLCVWAMEQ 151



 Score = 39.9 bits (89), Expect = 0.006
 Identities = 18/41 (43%), Positives = 27/41 (65%), Gaps = 2/41 (4%)

Query: 556 RYYHVFDEGEL-DQLCRDARLA-IVRSFYEEGNWCVVCMKV 594
           R+YHVF +GEL D + R+   A +++ FY  GNW +V  K+
Sbjct: 489 RFYHVFRKGELTDLITRNISTAGVIQEFYHHGNWVIVVEKL 529


>SB_57673| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 508

 Score = 49.2 bits (112), Expect = 9e-06
 Identities = 41/162 (25%), Positives = 76/162 (46%), Gaps = 7/162 (4%)

Query: 123 PNPKGLHIIPDFLTEDEEKLFLNTFNIQHVETTLKNRQVKHYGYEFRYGSNDVDLSCPLQ 182
           P+  G+ +I DF+++ EE   +   +    + +   R+ + YG +  +    V +S    
Sbjct: 75  PSLDGITLIEDFVSQREEARIVQVIDETVWKPSQSGRRKQDYGPQVNFKKKKVKMSH--F 132

Query: 183 EKIPKICKLLWRRLQHY--GYDLGVPVQLTVNKY--LPGQGIPSHVDKHSPFGETILALS 238
             +P   + L RR+     G    VPV+L   +Y    G  I +H D    +GE ++ L+
Sbjct: 133 NGLPAFSEFLVRRMNDDVPGLKDFVPVELCNLEYDEARGSSIDAHFDDFWLWGERLVTLN 192

Query: 239 LGSNVVMDWKHHTGKYVPVMVEARSMMIMQDEARYDWQHGIQ 280
           L S   +     T + + V +  RS++I+   AR+ WQH ++
Sbjct: 193 LLSATRLTMTKDTYE-ISVPMPRRSLIIVSGAARHLWQHAVK 233


>SB_58733| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2060

 Score = 44.0 bits (99), Expect = 3e-04
 Identities = 38/138 (27%), Positives = 66/138 (47%), Gaps = 28/138 (20%)

Query: 206 PVQLTVNKYLPGQGIPSHVDKHSPFGETILALSLGSNVVMDWKH---------------- 249
           P  + VN+Y PGQGI  H D    F   +  +SLGS+ ++D+ H                
Sbjct: 60  PNHVLVNEYEPGQGIMPHED-GPLFFPVVSTISLGSHTLLDFYHPLKKNTESQPTGMSHE 118

Query: 250 HTGK--------YVPVMVEARSMMIMQDEARYDWQHGIQPRMWDPVLDVRLIDNEKVKVI 301
            T K        ++ +++E RS++++ ++    + HGI+ R  D +    +++ E+ K+ 
Sbjct: 119 ETSKVTTLQDRYFMSLLLEPRSLLLLTEDLYTSYLHGIEGRAHD-IFTTDIVNREQCKLS 177

Query: 302 --TSDTVQREMRISLTFR 317
                T+ R  RISLT R
Sbjct: 178 KELGSTLIRSTRISLTIR 195


>SB_49517| Best HMM Match : Hydrolase (HMM E-Value=2.7e-27)
          Length = 228

 Score = 29.9 bits (64), Expect = 6.1
 Identities = 15/59 (25%), Positives = 31/59 (52%), Gaps = 5/59 (8%)

Query: 323 PCKCEYKMLCDSIERNIPETISN-----DVASNIEDIHVHQVYEQIAGHFSTTRHKPWP 376
           P +C  K + ++ +R IP  +++      V   ++D  + ++++ I G+   T HKP P
Sbjct: 95  PIQCVLKYVHEARKRGIPVAVASGSSKKQVEKALKDTGILELFDVILGNEDYTNHKPHP 153


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.322    0.138    0.431 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,006,773
Number of Sequences: 59808
Number of extensions: 940991
Number of successful extensions: 1852
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1836
Number of HSP's gapped (non-prelim): 11
length of query: 594
length of database: 16,821,457
effective HSP length: 86
effective length of query: 508
effective length of database: 11,677,969
effective search space: 5932408252
effective search space used: 5932408252
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 63 (29.5 bits)