BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001051-TA|BGIBMGA001051-PA|IPR004045|Glutathione S-transferase, N-terminal, IPR012336|Thioredoxin-like fold (68 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 37 0.001 At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 37 0.001 At2g02380.1 68415.m00176 glutathione S-transferase, putative sim... 36 0.004 At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 34 0.010 At2g30860.1 68415.m03761 glutathione S-transferase, putative ide... 34 0.013 At1g02930.1 68414.m00260 glutathione S-transferase, putative sim... 33 0.018 At3g62760.1 68416.m07050 glutathione S-transferase, putative Glu... 33 0.023 At1g02920.1 68414.m00259 glutathione S-transferase, putative sim... 31 0.071 At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden... 30 0.22 At2g02930.1 68415.m00241 glutathione S-transferase, putative 30 0.22 At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 29 0.29 At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 29 0.29 At4g02520.1 68417.m00345 glutathione S-transferase, putative 29 0.38 At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 29 0.38 At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 29 0.50 At5g16710.1 68418.m01956 dehydroascorbate reductase, putative St... 28 0.67 At2g45770.1 68415.m05693 signal recognition particle receptor pr... 26 2.7 At5g40720.1 68418.m04942 expressed protein contains Pfam profile... 25 6.2 At4g04950.1 68417.m00719 thioredoxin family protein similar to P... 25 8.2 At1g01410.1 68414.m00056 hypothetical protein 25 8.2 >At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 191 Score = 37.1 bits (82), Expect = 0.001 Identities = 23/65 (35%), Positives = 32/65 (49%), Gaps = 1/65 (1%) Query: 3 LTLYKMDASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTDDK 62 L LY S V + + AL + +Y+ V+LL D +F K+NP T+P L D Sbjct: 9 LKLYSYWRSSCAHRVRIAL-ALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPALVDGD 67 Query: 63 FVIWD 67 VI D Sbjct: 68 VVIND 72 >At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 221 Score = 37.1 bits (82), Expect = 0.001 Identities = 23/65 (35%), Positives = 32/65 (49%), Gaps = 1/65 (1%) Query: 3 LTLYKMDASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTDDK 62 L LY S V + + AL + +Y+ V+LL D +F K+NP T+P L D Sbjct: 9 LKLYSYWRSSCAHRVRIAL-ALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPALVDGD 67 Query: 63 FVIWD 67 VI D Sbjct: 68 VVIND 72 >At2g02380.1 68415.m00176 glutathione S-transferase, putative similar to gi:167970 gb:AAA72320 gb:AY052332 Length = 223 Score = 35.5 bits (78), Expect = 0.004 Identities = 16/38 (42%), Positives = 22/38 (57%) Query: 30 KYVDVDLLAEDHLKEEFLKLNPQHTIPMLTDDKFVIWD 67 +Y+ V+LL D +F K+NP T+P L D VI D Sbjct: 38 EYIPVNLLKGDQSDSDFKKINPMGTVPALVDGDVVIND 75 >At2g30870.1 68415.m03762 glutathione S-transferase, putative supported by cDNA GI:443698 GB:D17673 Length = 215 Score = 34.3 bits (75), Expect = 0.010 Identities = 21/67 (31%), Positives = 37/67 (55%), Gaps = 2/67 (2%) Query: 1 MVLTLYKMDASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTD 60 MVLT+Y + RAV ++E + + V+VDL+ + + E+L + P IP+L D Sbjct: 1 MVLTIYAPLFASSKRAVVTLVE--KGVSFETVNVDLMKGEQRQPEYLAIQPFGKIPVLVD 58 Query: 61 DKFVIWD 67 + I++ Sbjct: 59 GDYKIFE 65 >At2g30860.1 68415.m03761 glutathione S-transferase, putative identical to GB:Y12295 Length = 215 Score = 33.9 bits (74), Expect = 0.013 Identities = 20/67 (29%), Positives = 36/67 (53%), Gaps = 2/67 (2%) Query: 1 MVLTLYKMDASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTD 60 MVL +Y + P RA+ +IE + + + VDL+ +H + +L L P T+P + D Sbjct: 1 MVLKVYGPHFASPKRALVTLIEK-GVA-FETIPVDLMKGEHKQPAYLALQPFGTVPAVVD 58 Query: 61 DKFVIWD 67 + I++ Sbjct: 59 GDYKIFE 65 >At1g02930.1 68414.m00260 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 208 Score = 33.5 bits (73), Expect = 0.018 Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 1/58 (1%) Query: 10 ASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTDDKFVIWD 67 AS R V + + N+ + ++V V+L +H KE F+ NP +P D F I++ Sbjct: 11 ASTATRRVLIALHEKNV-DFEFVHVELKDGEHKKEPFILRNPFGKVPAFEDGDFKIFE 67 >At3g62760.1 68416.m07050 glutathione S-transferase, putative Glutathione transferase III(b) - Zea mays, EMBL:AJ010296 Length = 219 Score = 33.1 bits (72), Expect = 0.023 Identities = 20/67 (29%), Positives = 31/67 (46%), Gaps = 1/67 (1%) Query: 1 MVLTLYKMDASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTD 60 M + LY + S V V + + N + V V+L A H FL +NP +P L D Sbjct: 1 MAMKLYGDEMSACVARVLLCLHEKNT-EFELVPVNLFACHHKLPSFLSMNPFGKVPALQD 59 Query: 61 DKFVIWD 67 D +++ Sbjct: 60 DDLTLFE 66 >At1g02920.1 68414.m00259 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus]; supported by cDNA GI:443697. Length = 209 Score = 31.5 bits (68), Expect = 0.071 Identities = 16/58 (27%), Positives = 30/58 (51%), Gaps = 1/58 (1%) Query: 10 ASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTDDKFVIWD 67 AS R V + + N+ + ++V ++L +H KE F+ NP +P D F +++ Sbjct: 11 ASTATRRVLIALHEKNL-DFEFVHIELKDGEHKKEPFIFRNPFGKVPAFEDGDFKLFE 67 >At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) identical to GB:X95295. Based on identical cDNA hits, the translation is now 40 AAs longer at the N-terminal, and start of exon2 is also corrected. Length = 263 Score = 29.9 bits (64), Expect = 0.22 Identities = 18/69 (26%), Positives = 33/69 (47%), Gaps = 2/69 (2%) Query: 1 MVLTLYKMDASPPVRAVYMVIEALNIPNVKY--VDVDLLAEDHLKEEFLKLNPQHTIPML 58 +++ K+ P A V+ L ++++ + VD+ A H +E L LNP IP L Sbjct: 47 IIMASIKVHGVPMSTATMRVLATLYEKDLQFELIPVDMRAGAHKQEAHLALNPFGQIPAL 106 Query: 59 TDDKFVIWD 67 D +++ Sbjct: 107 EDGDLTLFE 115 >At2g02930.1 68415.m00241 glutathione S-transferase, putative Length = 212 Score = 29.9 bits (64), Expect = 0.22 Identities = 17/58 (29%), Positives = 28/58 (48%), Gaps = 1/58 (1%) Query: 10 ASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTDDKFVIWD 67 AS R V + + N+ + + V V+L +H KE FL NP +P D +++ Sbjct: 11 ASTSTRRVLIALHEKNL-DFELVHVELKDGEHKKEPFLSRNPFGQVPAFEDGDLKLFE 67 >At1g02950.2 68414.m00263 glutathione S-transferase, putative similar to glutathione-S-transferase GI:169887 from [Silene vulgaris] Length = 245 Score = 29.5 bits (63), Expect = 0.29 Identities = 17/64 (26%), Positives = 28/64 (43%), Gaps = 2/64 (3%) Query: 6 YKMDASPPVRAVYMVIEALNIPNVKY--VDVDLLAEDHLKEEFLKLNPQHTIPMLTDDKF 63 YK+ P V+ L+ + Y + V L +H E FL LNP +P+ D Sbjct: 27 YKVHGDPFSTNTRRVLAVLHEKRLSYEPITVKLQTGEHKTEPFLSLNPFGQVPVFEDGSV 86 Query: 64 VIWD 67 +++ Sbjct: 87 KLYE 90 >At1g02950.1 68414.m00262 glutathione S-transferase, putative similar to glutathione-S-transferase GI:169887 from [Silene vulgaris] Length = 243 Score = 29.5 bits (63), Expect = 0.29 Identities = 17/64 (26%), Positives = 28/64 (43%), Gaps = 2/64 (3%) Query: 6 YKMDASPPVRAVYMVIEALNIPNVKY--VDVDLLAEDHLKEEFLKLNPQHTIPMLTDDKF 63 YK+ P V+ L+ + Y + V L +H E FL LNP +P+ D Sbjct: 25 YKVHGDPFSTNTRRVLAVLHEKRLSYEPITVKLQTGEHKTEPFLSLNPFGQVPVFEDGSV 84 Query: 64 VIWD 67 +++ Sbjct: 85 KLYE 88 >At4g02520.1 68417.m00345 glutathione S-transferase, putative Length = 212 Score = 29.1 bits (62), Expect = 0.38 Identities = 17/58 (29%), Positives = 28/58 (48%), Gaps = 1/58 (1%) Query: 10 ASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLTDDKFVIWD 67 AS R V + + N+ + + V V+L +H KE FL NP +P D +++ Sbjct: 11 ASIATRRVLIALHEKNL-DFELVHVELKDGEHKKEPFLSRNPFGQVPAFEDGDLKLFE 67 >At1g02940.1 68414.m00261 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 256 Score = 29.1 bits (62), Expect = 0.38 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 2/57 (3%) Query: 6 YKMDASPPVRAVYMVIEALNIPNVKY--VDVDLLAEDHLKEEFLKLNPQHTIPMLTD 60 YK+ P V+ L+ + Y + V+L+A D K FL +NP +P+ D Sbjct: 39 YKIYGYPYSTNTRRVLAVLHEKGLSYDPITVNLIAGDQKKPSFLAINPFGQVPVFLD 95 >At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 228 Score = 28.7 bits (61), Expect = 0.50 Identities = 23/72 (31%), Positives = 33/72 (45%), Gaps = 8/72 (11%) Query: 3 LTLYKMDASPPVRAVYMVIEALNIPNVKYVDVDLLAEDHLK-------EEFLKLNPQHTI 55 L LY S V + + AL + +Y+ V+LL D ++F K+NP T+ Sbjct: 9 LKLYSYWRSSCAHRVRIAL-ALKGLDYEYIPVNLLKGDQFDSVYRFDLQDFKKINPMGTV 67 Query: 56 PMLTDDKFVIWD 67 P L D VI D Sbjct: 68 PALVDGDVVIND 79 >At5g16710.1 68418.m01956 dehydroascorbate reductase, putative Strong similarity to dehydroascorbate reductase [Spinacia oleracea] gi:10952512 gb:AAG24945 Length = 258 Score = 28.3 bits (60), Expect = 0.67 Identities = 19/65 (29%), Positives = 37/65 (56%), Gaps = 7/65 (10%) Query: 2 VLTLYKMDASPPVRAVYMVIEALNIP-NVKYVDVDLLAEDHLKEEFLKLNPQHTIPMLT- 59 + T K+ P + V + +E N+P ++K VD+ + E FLK++P+ +P++ Sbjct: 57 ITTPNKLGDCPFCQKVLLTMEEKNVPYDMKMVDLS-----NKPEWFLKISPEGKVPVVKF 111 Query: 60 DDKFV 64 D+K+V Sbjct: 112 DEKWV 116 >At2g45770.1 68415.m05693 signal recognition particle receptor protein, chloroplast (FTSY) similar to Cell division protein ftsY homolog (SP:O67066) {Aquifex aeolicus}; contains Pfam PF00448: SRP54-type protein, GTPase domain contains TIGRFAM TIGR00064: signal recognition particle-docking protein FtsY contains Pfam PF02881: SRP54-type protein, helical bundle domain; identical to cDNA chloroplast FtsY homolog GI:4583547 Length = 366 Score = 26.2 bits (55), Expect = 2.7 Identities = 16/38 (42%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Query: 3 LTLYKMDASPPVRAVYMVIEALNIPNVKYVDVDLLAED 40 L L K+D S V V+E L IP VK++ V ED Sbjct: 315 LILTKLDGSARGGCVVSVVEELGIP-VKFIGVGEAVED 351 >At5g40720.1 68418.m04942 expressed protein contains Pfam profile PF01697: Domain of unknown function Length = 583 Score = 25.0 bits (52), Expect = 6.2 Identities = 9/34 (26%), Positives = 20/34 (58%) Query: 34 VDLLAEDHLKEEFLKLNPQHTIPMLTDDKFVIWD 67 ++++ HLKEEF ++ + ++ K+ +WD Sbjct: 444 INVVHHFHLKEEFAFVDVDKSTMVINHYKYQVWD 477 >At4g04950.1 68417.m00719 thioredoxin family protein similar to PKCq-interacting protein PICOT from [Mus musculus] GI:6840949, [Rattus norvegicus] GI:6840951; contains Pfam profile PF00085: Thioredoxin Length = 488 Score = 24.6 bits (51), Expect = 8.2 Identities = 11/39 (28%), Positives = 21/39 (53%) Query: 20 VIEALNIPNVKYVDVDLLAEDHLKEEFLKLNPQHTIPML 58 V++ L NV + D+L+++ ++E K + T P L Sbjct: 185 VVDILKEVNVDFGSFDILSDNEVREGLKKFSNWPTFPQL 223 >At1g01410.1 68414.m00056 hypothetical protein Length = 361 Score = 24.6 bits (51), Expect = 8.2 Identities = 10/17 (58%), Positives = 13/17 (76%) Query: 37 LAEDHLKEEFLKLNPQH 53 +A+D LK+EFL L QH Sbjct: 52 IADDFLKDEFLDLIAQH 68 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.323 0.140 0.417 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,633,763 Number of Sequences: 28952 Number of extensions: 51003 Number of successful extensions: 141 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 18 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 121 Number of HSP's gapped (non-prelim): 22 length of query: 68 length of database: 12,070,560 effective HSP length: 48 effective length of query: 20 effective length of database: 10,680,864 effective search space: 213617280 effective search space used: 213617280 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (22.0 bits) S2: 51 (24.6 bits)
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