Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001044-TA|BGIBMGA001044-PA|IPR002048|Calcium-binding
EF-hand
(171 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 57 1e-09
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 48 7e-07
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 46 4e-06
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 33 0.030
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 32 0.053
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 31 0.092
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 29 0.37
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 1.1
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 2.0
SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces pomb... 26 2.6
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 57.2 bits (132), Expect = 1e-09
Identities = 39/127 (30%), Positives = 65/127 (51%), Gaps = 8/127 (6%)
Query: 11 FRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDFGALW-K 69
F D+D+ G I+++EL + + +P E ++ MI D G I F +F + +
Sbjct: 18 FSLFDRDQDGNITSNELGVVMRSLGQSPTAAE-LQDMINEVDADGNGTIDFTEFLTMMAR 76
Query: 70 YVSDWQN------CFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMVQKFDRFGRG 123
+ D N F+ FD+D +G I EL + LT+ G RLS + V M+++ D G G
Sbjct: 77 KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDG 136
Query: 124 TILFDDF 130
I +++F
Sbjct: 137 VINYEEF 143
Score = 42.7 bits (96), Expect = 3e-05
Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 7/94 (7%)
Query: 71 VSDWQNCFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMVQKFDRFGRGTILFDDF 130
+++++ F FDRD GNI EL + + G + + M+ + D G GTI F +F
Sbjct: 11 IAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEF 70
Query: 131 IQCCVTLYTLT-------SAFRQYDSDQDGVITI 157
+ T AF+ +D D +G IT+
Sbjct: 71 LTMMARKMKDTDNEEEVREAFKVFDKDGNGYITV 104
Score = 35.1 bits (77), Expect = 0.006
Identities = 33/138 (23%), Positives = 60/138 (43%), Gaps = 21/138 (15%)
Query: 45 RLMIGMFDKQNRGVISFEDFGALWKYV------SDWQNCFRSFDRDNSGNIDRVELKNAL 98
R +FD+ G I+ + G + + + ++ Q+ D D +G ID E L
Sbjct: 15 REAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEF---L 71
Query: 99 TAFGYRLSD----DVVGIMVQKFDRFGRGTILFDDFIQCCVTL------YTLTSAFRQYD 148
T ++ D + V + FD+ G G I ++ +L + R+ D
Sbjct: 72 TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREAD 131
Query: 149 SDQDGVITIHYEQFLKMV 166
+D DGVI +YE+F +++
Sbjct: 132 TDGDGVI--NYEEFSRVI 147
Score = 31.1 bits (67), Expect = 0.092
Identities = 26/105 (24%), Positives = 45/105 (42%), Gaps = 6/105 (5%)
Query: 2 PSRDFLWNIFRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISF 61
P+ L ++ VD D +G I E ++ + N E VR +FDK G I+
Sbjct: 45 PTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITV 104
Query: 62 ED----FGALWKYVSDWQ--NCFRSFDRDNSGNIDRVELKNALTA 100
E+ +L + +S + + R D D G I+ E +++
Sbjct: 105 EELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISS 149
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 48.0 bits (109), Expect = 7e-07
Identities = 22/60 (36%), Positives = 36/60 (60%)
Query: 73 DWQNCFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMVQKFDRFGRGTILFDDFIQ 132
++ FR FD+DNSG I+ + + + G +LSD+ V +MVQ+ D G+ + DF+Q
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Score = 30.3 bits (65), Expect = 0.16
Identities = 17/54 (31%), Positives = 23/54 (42%), Gaps = 1/54 (1%)
Query: 11 FRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDF 64
FR DKD SGYI + + + V+LM+ D N G + DF
Sbjct: 84 FRVFDKDNSGYIETAKFADYMKT-LGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 45.6 bits (103), Expect = 4e-06
Identities = 26/92 (28%), Positives = 44/92 (47%), Gaps = 7/92 (7%)
Query: 73 DWQNCFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMVQKFDRFGRGTILFDDFIQ 132
D F+ FD D ID EL+ A+ A G+ V +++ FD+ G+G + +DF++
Sbjct: 38 DINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVR 97
Query: 133 CCV-------TLYTLTSAFRQYDSDQDGVITI 157
L + AF +D D+ G I++
Sbjct: 98 VMTEKIVERDPLEEIKRAFELFDDDETGKISL 129
Score = 43.2 bits (97), Expect = 2e-05
Identities = 33/128 (25%), Positives = 55/128 (42%), Gaps = 8/128 (6%)
Query: 11 FRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDFGALW-- 68
F+ D D+ I EL+ A+ +N E ++++ FDK +G + EDF +
Sbjct: 43 FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD-FDKTGKGYLQMEDFVRVMTE 101
Query: 69 KYVS-----DWQNCFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMVQKFDRFGRG 123
K V + + F FD D +G I L+ + D + M+++FD G
Sbjct: 102 KIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDG 161
Query: 124 TILFDDFI 131
I +FI
Sbjct: 162 EINEQEFI 169
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 32.7 bits (71), Expect = 0.030
Identities = 26/102 (25%), Positives = 42/102 (41%), Gaps = 6/102 (5%)
Query: 51 FDKQNRGVISFEDFGALWKYVSDWQNCFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVV 110
F K + F D A +YV F FD D +G ID E AL+ +D +
Sbjct: 48 FQKIYKQFFPFGDPSAFAEYV------FNVFDADKNGYIDFKEFICALSVTSRGELNDKL 101
Query: 111 GIMVQKFDRFGRGTILFDDFIQCCVTLYTLTSAFRQYDSDQD 152
Q +D G I +D+ ++ +Y + + + D+D
Sbjct: 102 IWAFQLYDLDNNGLISYDEMLRIVDAIYKMVGSMVKLPEDED 143
Score = 31.1 bits (67), Expect = 0.092
Identities = 17/59 (28%), Positives = 33/59 (55%), Gaps = 4/59 (6%)
Query: 5 DFLWNIFRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFED 63
++++N+F D D++GYI E ALS + N + + ++D N G+IS+++
Sbjct: 66 EYVFNVF---DADKNGYIDFKEFICALSVTSRGELNDKLI-WAFQLYDLDNNGLISYDE 120
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 31.9 bits (69), Expect = 0.053
Identities = 13/48 (27%), Positives = 24/48 (50%)
Query: 71 VSDWQNCFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMVQKFD 118
+ + + F D+D GNI R ++K LT+ S+D + M + +
Sbjct: 47 IQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 31.1 bits (67), Expect = 0.092
Identities = 18/55 (32%), Positives = 32/55 (58%), Gaps = 1/55 (1%)
Query: 78 FRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMVQKFDRFGRGTILFDDFIQ 132
F+ FD+D +G I EL+ LT+ G +LS++ + ++ K G + + DF+Q
Sbjct: 83 FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL-KGVPVKDGMVNYHDFVQ 136
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 29.1 bits (62), Expect = 0.37
Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 4/58 (6%)
Query: 11 FRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGM----FDKQNRGVISFEDF 64
F+ D DR GYIS EL L + ++ ++ DK G ISFE+F
Sbjct: 99 FKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEF 156
Score = 28.7 bits (61), Expect = 0.49
Identities = 24/86 (27%), Positives = 37/86 (43%), Gaps = 11/86 (12%)
Query: 78 FRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMVQKFDRFGRGTILFDDFIQCCVTL 137
F D + SG+IDR E + + L+ + ++ D G G + F +FI
Sbjct: 30 FIKIDANQSGSIDRNEFLSIPSVASNPLASRLFSVV----DEDGGGDVDFQEFINSLSVF 85
Query: 138 YT-------LTSAFRQYDSDQDGVIT 156
L AF+ YD D+DG I+
Sbjct: 86 SVHGNKEEKLKFAFKIYDIDRDGYIS 111
Score = 27.1 bits (57), Expect = 1.5
Identities = 19/84 (22%), Positives = 34/84 (40%), Gaps = 2/84 (2%)
Query: 20 GYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDFGALWKYVSD--WQNC 77
G + + +SN +++ E +R D G I +F ++ S+
Sbjct: 2 GQSQSQIFEDLISNSSFSNEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLASRL 61
Query: 78 FRSFDRDNSGNIDRVELKNALTAF 101
F D D G++D E N+L+ F
Sbjct: 62 FSVVDEDGGGDVDFQEFINSLSVF 85
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.5 bits (58), Expect = 1.1
Identities = 17/62 (27%), Positives = 31/62 (50%), Gaps = 3/62 (4%)
Query: 11 FRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDFGALWKY 70
F D GYI ++L+++ + N E ++LM+ + G +S E+F LW +
Sbjct: 18 FDLFDVTHKGYIDFEDLRRSCAQLGEN-LTKEQLQLMLDLAG--TNGKVSREEFAELWIH 74
Query: 71 VS 72
+S
Sbjct: 75 IS 76
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 2.0
Identities = 12/30 (40%), Positives = 17/30 (56%)
Query: 3 SRDFLWNIFRSVDKDRSGYISADELQQALS 32
S +FL F+ D D SG +S +E Q +S
Sbjct: 331 SMEFLEETFQKADADHSGKLSFEEFQHFVS 360
>SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 510
Score = 26.2 bits (55), Expect = 2.6
Identities = 21/80 (26%), Positives = 37/80 (46%), Gaps = 7/80 (8%)
Query: 30 ALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDFGALWKYVS-DWQNCFRSFDRDNSGN 88
A+ NG NP + L + +G++ FE L +Y+ W C +FD+ SG+
Sbjct: 200 AIGNGWINPLSHYETYLNYLV----EKGMVDFES--ELGQYLHHSWAECLLAFDKIGSGS 253
Query: 89 IDRVELKNALTAFGYRLSDD 108
D + ++ L Y +S +
Sbjct: 254 GDLSKCESFLGDILYMVSKE 273
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.326 0.141 0.445
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 837,464
Number of Sequences: 5004
Number of extensions: 35136
Number of successful extensions: 136
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 112
Number of HSP's gapped (non-prelim): 21
length of query: 171
length of database: 2,362,478
effective HSP length: 68
effective length of query: 103
effective length of database: 2,022,206
effective search space: 208287218
effective search space used: 208287218
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.6 bits)
S2: 51 (24.6 bits)
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