BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001041-TA|BGIBMGA001041-PA|IPR000873|AMP-dependent synthetase and ligase, IPR002372|Pyrrolo-quinoline quinone, IPR011047|Quinonprotein alcohol dehydrogenase-like, IPR009081|Acyl carrier protein-like, IPR006163|Phosphopantetheine-binding (758 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 54 7e-08 SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 37 0.012 SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 36 0.016 SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 32 0.34 SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||... 31 0.45 SPBC887.04c |lub1||WD repeat protein Lub1|Schizosaccharomyces po... 31 0.59 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 31 0.78 SPAC2G11.13 |atg22||autophagy associated protein Atg22 |Schizosa... 31 0.78 SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 30 1.0 SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4 |S... 30 1.0 SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein Rrp12|Sch... 30 1.4 SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1... 29 2.4 SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 29 3.2 SPBC1306.02 ||SPBC4.08|WD repeat protein, human WDR6 family|Schi... 27 7.3 SPCC306.06c |||ER membrane protein, BIG1 family |Schizosaccharom... 27 9.6 SPAC212.03 |||hypothetical protein|Schizosaccharomyces pombe|chr... 27 9.6 SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|c... 27 9.6 SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||M... 27 9.6 SPCC5E4.03c |taf72||transcription factor TFIID complex subunit 5... 27 9.6 SPAC25H1.08c |||ribosome biogenesis protein Sqt1|Schizosaccharom... 27 9.6 >SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 512 Score = 54.0 bits (124), Expect = 7e-08 Identities = 38/137 (27%), Positives = 66/137 (48%), Gaps = 8/137 (5%) Query: 157 LNRKNGNKEE-NSIKFIDTGDIGEI-RNGTVFYRGRVDDTIKRFGHKINLHSIESTVMQC 214 LN NK +F TGD G++ ++G VF GR+ + + R G KI+ I++ +MQ Sbjct: 368 LNNPAANKSSFTKDRFFRTGDEGKLDKDGYVFITGRIKELVNRGGEKISPAEIDAVLMQH 427 Query: 215 PRVRSCSCV------WLQTPLLLIVYFSAETLSSQELLDFLKCKLDEKYWPDKVIRVDTL 268 P V C + Q I + +T++ ++L D+L+ K+ P K D + Sbjct: 428 PDVSEAVCFAVPDEKYGQDIQAAINPVAGKTVTPKQLHDYLEQKVAAFKIPKKFYFTDRI 487 Query: 269 PTNAHGKTSKEILVNIY 285 P A GK + ++ + + Sbjct: 488 PKTATGKVQRRLVCDAF 504 >SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces pombe|chr 1|||Manual Length = 4924 Score = 36.7 bits (81), Expect = 0.012 Identities = 32/118 (27%), Positives = 54/118 (45%), Gaps = 9/118 (7%) Query: 166 ENSIKFIDTGDIGEIRNGTVFYRGRV--DDTIKRFGHKINLHSIESTVMQCPRVRSCSCV 223 E ++ TGD+G I NG ++ GR+ D IK G +I + IES + P V S + Sbjct: 587 EQTLSIYRTGDLGRIINGKLYIFGRIAGDMQIKIRGRRIEIGEIESKL--APSVDSLAIE 644 Query: 224 WLQTPLLLIVYFSAETLSSQELLDFLKCKLDEKYWPDKVIRVDTLPTNAHGKTSKEIL 281 + L+ ++ + + +L + L P K + + P A GKT ++ L Sbjct: 645 KIGDN--LVAFYVGDEM---KLRKHAESHLASWMCPTKYVGLPGFPRLASGKTDRKAL 697 Score = 29.5 bits (63), Expect = 1.8 Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 2/40 (5%) Query: 174 TGDIGEIR--NGTVFYRGRVDDTIKRFGHKINLHSIESTV 211 TGD+G + N ++ + GR DD IK G +I + I + + Sbjct: 3098 TGDLGRLLKDNNSLEFCGRTDDQIKLRGQRIEIGEINAVI 3137 >SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 36.3 bits (80), Expect = 0.016 Identities = 36/125 (28%), Positives = 56/125 (44%), Gaps = 13/125 (10%) Query: 171 FIDTGDIGEIRN--GTVFYRGRVDDTIKRFGHKINLHSIESTVMQCPRVRSCSCVWLQTP 228 F TGD G R+ G ++ RGRVDD + GH+++ IE+ ++ V + V + Sbjct: 505 FYFTGD-GATRDKDGYIWIRGRVDDVVNISGHRLSTAEIEAALLSHDAVAESAVVGVHDE 563 Query: 229 L---------LLIVYFSAETLSSQELLDFLKCKLDEKYWPDKVIRVDTLPTNAHGKTSKE 279 L LL + A +EL+ ++ + P K+I D LP GK + Sbjct: 564 LTGQAVNAFILLKPGYEATVELEKELIMAVRSTIGPFASPRKLIFSD-LPKTRSGKIMRR 622 Query: 280 ILVNI 284 IL I Sbjct: 623 ILRKI 627 >SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1419 Score = 31.9 bits (69), Expect = 0.34 Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Query: 174 TGDIGE-IRNGTVFYRGRVDDTIKRFGHKINLHSIESTVMQCPRVR 218 +GD+G + G V GR DD IK G +I L I + + + P VR Sbjct: 723 SGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVR 768 Score = 30.3 bits (65), Expect = 1.0 Identities = 25/110 (22%), Positives = 51/110 (46%), Gaps = 9/110 (8%) Query: 244 ELLDFLKCKLDEKYWPDKVIRVDTLPTNAHGKTSKEIL-------VNIYKKSNILQNVEN 296 ++ ++LK KL P ++ + +P N +GK K L + +S+ V+ Sbjct: 821 DIREYLKTKLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDE 880 Query: 297 IKSYLFKELKALVNKDLNY-EDLQSK-SFFSLGGTSFLAVTICNKLSLTF 344 + ++++ + + + + D+ K SFF +GG S LA + +L F Sbjct: 881 TLTATERDIRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKF 930 >SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1101 Score = 31.5 bits (68), Expect = 0.45 Identities = 32/124 (25%), Positives = 56/124 (45%), Gaps = 15/124 (12%) Query: 268 LPTNAHGKTSKEILVNIYKKSNI----LQNVENIKSYLFKELKALVNKDLNYEDLQSKSF 323 LP K+SK++L +Y+ + + L + + S E +A+++ DLN+ED+ K F Sbjct: 258 LPLRDLAKSSKKLL-GLYELNTLFPPCLNKLIQLNSAFLDEFEAIMS-DLNFEDIDEKKF 315 Query: 324 FSLGGTSFLAVTICNKLSLTFPEVGKHILPHLLSHNKSIGDVLKTITQDTNCLENKFKKS 383 + L + C F +H P L + G+VLK ++ +E F Sbjct: 316 EEID----LRLACC--FESHFFAFSQH-YPRYLEQSNDFGNVLKMASKIPKFVE--FHDQ 366 Query: 384 IKRN 387 +K N Sbjct: 367 VKLN 370 >SPBC887.04c |lub1||WD repeat protein Lub1|Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 31.1 bits (67), Expect = 0.59 Identities = 44/192 (22%), Positives = 85/192 (44%), Gaps = 19/192 (9%) Query: 529 YIASYDGNIRCIDILTSTIKETVHVSDQA-ISADLVLAKNEFILFGTLSGVCACLHVNTK 587 + +++G + C+ + + K + D+ I ++ + LFG S +C+ +N++ Sbjct: 51 FYENHEGFVNCVCYVPAIDKNSRGGQDKCGILQEVGTNSPSYYLFGHESNICSASALNSE 110 Query: 588 TII---WQGSLK-GPIFACPVLYDNDKYVIFAEVN-GE--IHCRTVEKGIKIWTYNG--- 637 TII W + + + C + + ++A + GE + +K IKIW NG Sbjct: 111 TIITGSWDSTARVWALGQCKYVLKGHQSSVWAVLALGEDIFITGSADKLIKIW--NGEKL 168 Query: 638 AKGNIFSSLCVKEINKFKWQMYFGCHDNNVYSVNITN--FQPSLHWKTRVSSPVYSTPTI 695 K + + CV+ + + + C ++ V + ++ F LH T S VYS I Sbjct: 169 VKSILAHNDCVRSLCQIPGG-FASCSNDGVIKLWTSDGEFLYELHGHT---SFVYSLTYI 224 Query: 696 FNDKLILAASTD 707 N +LI + D Sbjct: 225 HNQQLIASCGED 236 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 30.7 bits (66), Expect = 0.78 Identities = 14/40 (35%), Positives = 28/40 (70%), Gaps = 2/40 (5%) Query: 265 VDTLPTNAHGKTSKEILVNIYKKSNILQNVENIKSYLFKE 304 VD+LPT+ H K S +IL +++ +++L ++ + +YL K+ Sbjct: 880 VDSLPTSKHIKNSNDILSSVFTINDML--IDEMTAYLSKK 917 >SPAC2G11.13 |atg22||autophagy associated protein Atg22 |Schizosaccharomyces pombe|chr 1|||Manual Length = 529 Score = 30.7 bits (66), Expect = 0.78 Identities = 18/43 (41%), Positives = 20/43 (46%), Gaps = 2/43 (4%) Query: 217 VRSCSCVW--LQTPLLLIVYFSAETLSSQELLDFLKCKLDEKY 257 V CSC W L TPL IV E SS +L L + E Y Sbjct: 275 VTVCSCWWLILSTPLCTIVTLPVENHSSDAILTLLYNSVKESY 317 >SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 30.3 bits (65), Expect = 1.0 Identities = 23/86 (26%), Positives = 41/86 (47%), Gaps = 2/86 (2%) Query: 243 QELLDFLKCKLDEKYWPDKVIRVDTLPTNAHGKTSKEILVNIYKKSNILQNVENIKS--Y 300 +EL LK ++ + + L + H S IYK +LQN E +KS Y Sbjct: 1774 EELFSSLKSSEEDFALRTRCVTSLMLVNSKHPSMSALCRFIIYKSVTMLQNGEVLKSGDY 1833 Query: 301 LFKELKALVNKDLNYEDLQSKSFFSL 326 + + + AL++ ++ + + KSF S+ Sbjct: 1834 VTEFIPALLDMHVHIPEEKKKSFLSM 1859 >SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 517 Score = 30.3 bits (65), Expect = 1.0 Identities = 23/54 (42%), Positives = 30/54 (55%), Gaps = 4/54 (7%) Query: 272 AHGKTSKEILVNIYKKS---NILQNVENIKSYLFKELKALVNKDLNYEDLQSKS 322 AHGK K IL K+S N QNV N+ S E +AL+ ++ N E+L S S Sbjct: 10 AHGKPKKVILSLSLKESSKINDSQNVSNVSSKEKCETEALLREE-NKENLSSDS 62 >SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein Rrp12|Schizosaccharomyces pombe|chr 2|||Manual Length = 1001 Score = 29.9 bits (64), Expect = 1.4 Identities = 27/91 (29%), Positives = 40/91 (43%), Gaps = 12/91 (13%) Query: 279 EILVNIYKKSNILQ----NVENIKSYLFKELKALVNKDLNYEDL--QSKSFFSLGGTSFL 332 + V++ K N+ Q N N+ L L + N D N EDL + S FS+ G L Sbjct: 537 QAFVDLTKIENVRQLISSNSSNLIGVLGNTLATMKNDDSNAEDLVMAATSIFSVAGDQQL 596 Query: 333 AV--TICNKLSLTFPEVGK----HILPHLLS 357 + +C L+ P +G + P LLS Sbjct: 597 QIITNLCESAPLSEPSLGSTGVIKLFPALLS 627 >SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1023 Score = 29.1 bits (62), Expect = 2.4 Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 2/61 (3%) Query: 651 INKFKWQMYFGCHDNNVYSVNITNFQPSLHWKTRVSSPVYSTPTIFNDKLILAASTDGKL 710 I F + FG H N + ++ +P L + T SP Y+ P + N K + A T + Sbjct: 639 IADFGFATTFG-HFNTLSEHSLPTEKPDL-FSTSCGSPCYAAPELVNCKSGMYAGTQADI 696 Query: 711 W 711 W Sbjct: 697 W 697 >SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 28.7 bits (61), Expect = 3.2 Identities = 10/29 (34%), Positives = 21/29 (72%) Query: 541 DILTSTIKETVHVSDQAISADLVLAKNEF 569 D++ + I+E +HVS++ + DL +A+ E+ Sbjct: 176 DVINANIQEAIHVSEEFRNWDLEMAEKEY 204 >SPBC1306.02 ||SPBC4.08|WD repeat protein, human WDR6 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 984 Score = 27.5 bits (58), Expect = 7.3 Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 12/72 (16%) Query: 633 WTYNGAKGNIFSSLCVKEINKFKWQMYFGCHDNNVYSVNITNFQPSLHWKTRVSSPVYST 692 +TY G +G SL V N +Y G +D +V S + +KTR+ S Sbjct: 274 YTYTGHRGKHIWSLVVSSANPI---IYTGGNDGSVRSWD---------YKTRIQESCISV 321 Query: 693 PTIFNDKLILAA 704 ++F K +LA+ Sbjct: 322 SSLFTSKTLLAS 333 >SPCC306.06c |||ER membrane protein, BIG1 family |Schizosaccharomyces pombe|chr 3|||Manual Length = 311 Score = 27.1 bits (57), Expect = 9.6 Identities = 14/32 (43%), Positives = 20/32 (62%) Query: 223 VWLQTPLLLIVYFSAETLSSQELLDFLKCKLD 254 V LQ+P L++ +S E+L SQEL+ K D Sbjct: 88 VSLQSPYSLLLPYSKESLDSQELVHSAKQNCD 119 >SPAC212.03 |||hypothetical protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 129 Score = 27.1 bits (57), Expect = 9.6 Identities = 15/61 (24%), Positives = 27/61 (44%), Gaps = 1/61 (1%) Query: 85 NNKTRIFTLYGVTEMSCWASVAELDLNKVTTGDKEVPLGNCLSETELFVQPHEKNNT-MG 143 NN+ F Y + C +S+ ++ +T NC S + + Q ++ NT +G Sbjct: 50 NNRNLAFKAYDAVKQKCDSSIKVFNIQDITIKGATWQHHNCQSTGKWYSQLYDYQNTFIG 109 Query: 144 K 144 K Sbjct: 110 K 110 >SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 801 Score = 27.1 bits (57), Expect = 9.6 Identities = 17/53 (32%), Positives = 25/53 (47%) Query: 519 SKATYCKGVLYIASYDGNIRCIDILTSTIKETVHVSDQAISADLVLAKNEFIL 571 S AT G + +AS G+IR D + K + +AI V A +F+L Sbjct: 585 SAATTENGYIAVASNKGDIRLFDRIGVNAKTALPALGEAIIGVDVTASGDFVL 637 >SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 1115 Score = 27.1 bits (57), Expect = 9.6 Identities = 11/34 (32%), Positives = 18/34 (52%) Query: 426 CVDASPALYKSESVLYVTVGSHSGKIVVMNANTG 459 C+D Y+S + V +G + K+ V+NA G Sbjct: 126 CIDYRAMFYQSRNNPEVVIGGYHQKLTVVNAERG 159 >SPCC5E4.03c |taf72||transcription factor TFIID complex subunit 5 Taf72|Schizosaccharomyces pombe|chr 3|||Manual Length = 643 Score = 27.1 bits (57), Expect = 9.6 Identities = 20/78 (25%), Positives = 35/78 (44%), Gaps = 4/78 (5%) Query: 569 FILFGTLSGVCAC--LHVNTKTIIWQGSLKGPIFACPVLYDNDKYVIFAEVNGEIHCRTV 626 ++L G+ C +H ++ G + P+ A + D + A+ G IH + Sbjct: 477 YVLTGSSDKTCRLWDVHRGHSVRVFNGHTQ-PVTAVAIAPDGHT-MASADSEGLIHLWDI 534 Query: 627 EKGIKIWTYNGAKGNIFS 644 G +I T G +GNI+S Sbjct: 535 GTGRRIKTMRGHRGNIYS 552 >SPAC25H1.08c |||ribosome biogenesis protein Sqt1|Schizosaccharomyces pombe|chr 1|||Manual Length = 399 Score = 27.1 bits (57), Expect = 9.6 Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 3/60 (5%) Query: 444 VGSHSGKIVVMNANTGFIQGLIKLKSR-IEAPILCVH-DECV-TSPCGVVGTYDGTVICF 500 +G SGK+ V+N N+ I ++ ++ +EA LC C S G V YD + F Sbjct: 260 LGGSSGKVKVVNINSSHILASLEAQTESVEAIALCTALPICACASVDGTVALYDSASLKF 319 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.320 0.135 0.409 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,671,775 Number of Sequences: 5004 Number of extensions: 165428 Number of successful extensions: 433 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 412 Number of HSP's gapped (non-prelim): 31 length of query: 758 length of database: 2,362,478 effective HSP length: 78 effective length of query: 680 effective length of database: 1,972,166 effective search space: 1341072880 effective search space used: 1341072880 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 57 (27.1 bits)
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