Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001014-TA|BGIBMGA001014-PA|IPR005178|Protein of unknown
function DUF300
(421 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces pombe... 101 1e-22
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 32 0.13
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 30 0.53
SPBC29A3.15c |rsm23||mitochondrial ribosomal protein subunit S23... 30 0.70
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 30 0.70
SPBC25D12.02c |dnt1||nucleolar protein Dnt1|Schizosaccharomyces ... 29 1.2
SPBC336.15 |pic1|SPBC685.01|INCENP-like|Schizosaccharomyces pomb... 28 2.1
SPCC24B10.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3|... 27 3.7
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 27 4.9
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 4.9
SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha su... 27 4.9
SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit Mts4|... 27 4.9
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 6.5
SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 27 6.5
SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|... 27 6.5
SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like protein|Schi... 26 8.6
SPBC800.09 |sum2||G2/M transition checkpoint protein Sum2|Schizo... 26 8.6
>SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 426
Score = 101 bits (243), Expect = 1e-22
Identities = 64/218 (29%), Positives = 116/218 (53%), Gaps = 9/218 (4%)
Query: 65 ISIWQITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLRECYEAYV 124
+S I H+ +Y KP LQ+ ++RIL M+ IY+ + + + + + RE YEA+
Sbjct: 19 LSCISIITHLKNYKKPVLQRSVVRILMMIVIYSSVSFLSVYNEKIGSIFEPFREIYEAFA 78
Query: 125 IYNFMKYLLNYLNDGHDLEAVLETKPQV--YHIFPLCCLA-PWEMGREFVH-NCKHGILQ 180
+Y F L++YL G + AV+ + ++PL L ++ + + K GILQ
Sbjct: 79 LYCFFCLLIDYL--GGERAAVISLHGHLPRPRLWPLNYLQDDIDLSDPYTFLSIKRGILQ 136
Query: 181 YTLVRPITTVISMICELNDVYGESDFKPNVAFP--YIIAINNLSQFVAMYCLVLFYKANR 238
YT ++P + ++ ++ VY D +P A +I + N+S +++Y L F+
Sbjct: 137 YTWLKPFLVIAVLLTKVTGVYDRED-QPVYASADLWIGLVYNISITLSLYSLTTFWVCLH 195
Query: 239 TELKPMKPIGKFLCIKAVVFFSFFQGVIITILVYCGVL 276
EL P +P KFL +KA++F S++Q +++I + G+L
Sbjct: 196 EELAPFRPFPKFLSVKAIIFASYWQQTVLSITNWLGLL 233
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 32.3 bits (70), Expect = 0.13
Identities = 18/69 (26%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Query: 335 SFSRRLRGKSMYHMTR-GYDESSRLMGEPVPSSSAPNLTTETETPSVEVRPSAYGSIENT 393
S +R R K ++ + +RL P SSS P++ + + TPS+ P S+ N
Sbjct: 85 STRKRWRQKRLWSFKNFPFRPPTRLTSTPSNSSSLPSIPSSSSTPSISSIPHTTSSVSND 144
Query: 394 VTNMSMTSE 402
+ ++ +S+
Sbjct: 145 IPSVLGSSD 153
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 30.3 bits (65), Expect = 0.53
Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Query: 355 SSRLMGEPVPSSSAPNLTTETETPSVEVRPSAYGSIENTVTNMSMTS 401
SS ++ P S P++ T+TE+ S S+ S E+T+ N +S
Sbjct: 193 SSTILSSPSVEQSTPSIITQTES-STTTEGSSVASSESTIANSDQSS 238
Score = 27.5 bits (58), Expect = 3.7
Identities = 15/46 (32%), Positives = 23/46 (50%)
Query: 365 SSSAPNLTTETETPSVEVRPSAYGSIENTVTNMSMTSETEPLLDAS 410
S+S PNLTT +P + S+ I + + TS T + +AS
Sbjct: 292 STSGPNLTTSNTSPQISSTISSSSFIVESPSVALSTSSTTTITNAS 337
>SPBC29A3.15c |rsm23||mitochondrial ribosomal protein subunit
S23|Schizosaccharomyces pombe|chr 2|||Manual
Length = 476
Score = 29.9 bits (64), Expect = 0.70
Identities = 16/74 (21%), Positives = 33/74 (44%)
Query: 346 YHMTRGYDESSRLMGEPVPSSSAPNLTTETETPSVEVRPSAYGSIENTVTNMSMTSETEP 405
Y+ + Y + R +G + SSS +E P + P + S+E + T + + +
Sbjct: 48 YYSSSQYQHTGRPVGGNIHSSSNQQRQKNSEAPRINEIPPSTSSVEKSTTIPNSSVVLDH 107
Query: 406 LLDASDKSNDNENP 419
LL+ + + + P
Sbjct: 108 LLNEGENTLEEVKP 121
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 29.9 bits (64), Expect = 0.70
Identities = 33/151 (21%), Positives = 54/151 (35%), Gaps = 3/151 (1%)
Query: 274 GVLKTIFDISDTDTVKIISSKLQPYVSPLDASPSCLGSFLAMWDVSDVKRDISEHLGVVG 333
GVL+T S + SS P S + S S + S S
Sbjct: 121 GVLQTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSS 180
Query: 334 SSFSRRLRGKSMYHMTRGYDESSRLMGEPVPSSSAPN---LTTETETPSVEVRPSAYGSI 390
SS S S + SS P+ SS++ + ++ + + S RPS+ S
Sbjct: 181 SSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSRPSSSSSF 240
Query: 391 ENTVTNMSMTSETEPLLDASDKSNDNENPQA 421
T+++ + S +S S +E P +
Sbjct: 241 ITTMSSSTFISTVTVTPSSSSSSTSSEVPSS 271
>SPBC25D12.02c |dnt1||nucleolar protein Dnt1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 599
Score = 29.1 bits (62), Expect = 1.2
Identities = 17/58 (29%), Positives = 32/58 (55%)
Query: 364 PSSSAPNLTTETETPSVEVRPSAYGSIENTVTNMSMTSETEPLLDASDKSNDNENPQA 421
PS++ P+ TE+ + + S+ SI ++ + S TS+ +D+ +KS N+N A
Sbjct: 346 PSTAVPSENDTTESENDTLSESSTTSISSSPSENSDTSDDLTKVDSPNKSLVNDNVSA 403
>SPBC336.15 |pic1|SPBC685.01|INCENP-like|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1018
Score = 28.3 bits (60), Expect = 2.1
Identities = 20/72 (27%), Positives = 37/72 (51%), Gaps = 6/72 (8%)
Query: 343 KSMYHMTRGYDESSRLMGEPVPSSSAPNLTTETETPSVEVRPSAYGSIENTVTNMSMTSE 402
K+M+H + + + +M +PSSS T+++ P +E + S + + ++M S
Sbjct: 838 KTMHHNVNPFTKQNGIMKGKLPSSS----TSQSNKPFIE-KASMHAPAKGRNSSMQEPSS 892
Query: 403 TEPLLDASDKSN 414
PLL + KSN
Sbjct: 893 KSPLL-KTPKSN 903
>SPCC24B10.06 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 156
Score = 27.5 bits (58), Expect = 3.7
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 4/76 (5%)
Query: 248 GKFLCIKAVVFFSFFQGVIITILVYCGVLKTIFDISDTDTVKIISSK--LQPYVSPLDAS 305
GK + F QG T++ G L+ + +I T +++ SK L P+V D S
Sbjct: 3 GKVSSLLVFASFLIIQGAFATLVAPIGDLEHLSEIELLYTNRVLPSKIPLTPFVKRDDDS 62
Query: 306 PSCLGSFLAMWDVSDV 321
S S+ VSDV
Sbjct: 63 SSTQASYST--TVSDV 76
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 27.1 bits (57), Expect = 4.9
Identities = 11/23 (47%), Positives = 15/23 (65%)
Query: 286 DTVKIISSKLQPYVSPLDASPSC 308
DT K+I +L P +SPL P+C
Sbjct: 1151 DTCKMIEKRLWPTMSPLRQFPNC 1173
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 4.9
Identities = 16/54 (29%), Positives = 24/54 (44%)
Query: 365 SSSAPNLTTETETPSVEVRPSAYGSIENTVTNMSMTSETEPLLDASDKSNDNEN 418
SSS N + TE+ E + S T+ + S TS T L +S S+ +
Sbjct: 239 SSSLTNTVSPTESTFYETKSSTSSVPTQTIDSSSFTSSTPVSLTSSSTSSSGSS 292
>SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha
subunit Tfa1 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 448
Score = 27.1 bits (57), Expect = 4.9
Identities = 22/103 (21%), Positives = 44/103 (42%), Gaps = 8/103 (7%)
Query: 317 DVSDVKRDISEHLGVVGSSFSRRLRGKSMYHMTRGYDESSRLMGEPVPSSSAPNLTTETE 376
+V D K DI+ + + ++ LR K +ES + E V + T
Sbjct: 312 EVQDTKTDITSEKSALDAYYAT-LRAKQK-------EESEFMDSENVDDEEDDDFLDVTT 363
Query: 377 TPSVEVRPSAYGSIENTVTNMSMTSETEPLLDASDKSNDNENP 419
S++ + + YGS++ N++ S+ + S + N++ P
Sbjct: 364 ATSLQNKSTDYGSVKRKTENLNSDSDIQNKRTKSIEENNSLPP 406
>SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit
Mts4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 27.1 bits (57), Expect = 4.9
Identities = 16/70 (22%), Positives = 29/70 (41%)
Query: 316 WDVSDVKRDISEHLGVVGSSFSRRLRGKSMYHMTRGYDESSRLMGEPVPSSSAPNLTTET 375
W S K +++ L V+G S+S + +S+ + +G L G A + E
Sbjct: 114 WPQSPQKTQLADILSVLGMSYSNTSKHESLKYRLQGVTTDPSLWGHEYVRHLASEIEEEF 173
Query: 376 ETPSVEVRPS 385
+ E P+
Sbjct: 174 ASRQEEEAPT 183
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.6 bits (56), Expect = 6.5
Identities = 32/132 (24%), Positives = 52/132 (39%), Gaps = 10/132 (7%)
Query: 283 SDTDTVKIISSKLQPYVSP------LDASPSCLGSFLAMWDVSDVKRDISEHLGVVGSSF 336
S T T ISS SP + +S S SF + S + S SS
Sbjct: 282 SPTSTSSTISSSSSSSSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSS 341
Query: 337 ----SRRLRGKSMYHMTRGYDESSRLMGEPVPSSSAPNLTTETETPSVEVRPSAYGSIEN 392
S S + T +SS V SSS+ + +T T + S RP++ S +
Sbjct: 342 TISSSSSSPSSSSFSSTTSSSKSSSSFSSTVSSSSSTSSSTLTSSSSSSSRPASSSSHSS 401
Query: 393 TVTNMSMTSETE 404
++++ +S ++
Sbjct: 402 SLSSHKSSSSSK 413
>SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 978
Score = 26.6 bits (56), Expect = 6.5
Identities = 13/64 (20%), Positives = 31/64 (48%), Gaps = 6/64 (9%)
Query: 78 TKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLRECYEAYVIYNFMKYLLNYLN 137
T P+ +H+ +++ + L+ ++ + + C ++ +YN YL+ YL+
Sbjct: 287 TDPNFDEHVAKLINAQGV----ELVAIKSDPSELSPELKENC--SFQLYNLFPYLIRYLS 340
Query: 138 DGHD 141
D +D
Sbjct: 341 DDYD 344
>SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|chr
3|||Manual
Length = 521
Score = 26.6 bits (56), Expect = 6.5
Identities = 13/44 (29%), Positives = 24/44 (54%)
Query: 74 IVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLR 117
++HY +P L I +++V I A+N L F E +L +++
Sbjct: 149 VIHYWRPDLNVGIWVAVFLVVILAINLLHVKYFGEVEFWLSAVK 192
>SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 308
Score = 26.2 bits (55), Expect = 8.6
Identities = 16/57 (28%), Positives = 25/57 (43%)
Query: 363 VPSSSAPNLTTETETPSVEVRPSAYGSIENTVTNMSMTSETEPLLDASDKSNDNENP 419
V + +A + TE PS PSA + EN V +S +EP + + +P
Sbjct: 31 VNTLTAHTVHPSTELPSATSFPSALPNSENPVIQNISSSSSEPHHTSQSTPGETSSP 87
>SPBC800.09 |sum2||G2/M transition checkpoint protein
Sum2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 26.2 bits (55), Expect = 8.6
Identities = 17/69 (24%), Positives = 31/69 (44%), Gaps = 1/69 (1%)
Query: 337 SRRLRGKSMYHMTRGYD-ESSRLMGEPVPSSSAPNLTTETETPSVEVRPSAYGSIENTVT 395
S+ G++ Y++ GY S++ P AP T + +PS++ P G I
Sbjct: 188 SQGFAGQAPYNVRPGYGMPSNQKPPNFAPGMPAPGPTAVSASPSLQSMPPTNGVIPGAQP 247
Query: 396 NMSMTSETE 404
++ + E E
Sbjct: 248 SIEASIEKE 256
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.322 0.137 0.420
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,894,113
Number of Sequences: 5004
Number of extensions: 79837
Number of successful extensions: 251
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 232
Number of HSP's gapped (non-prelim): 23
length of query: 421
length of database: 2,362,478
effective HSP length: 75
effective length of query: 346
effective length of database: 1,987,178
effective search space: 687563588
effective search space used: 687563588
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 55 (26.2 bits)
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