Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001014-TA|BGIBMGA001014-PA|IPR005178|Protein of unknown
function DUF300
(421 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g26740.2 68418.m03164 expressed protein contains Pfam profile... 171 7e-43
At5g26740.1 68418.m03163 expressed protein contains Pfam profile... 171 7e-43
At3g05940.1 68416.m00676 expressed protein contains Pfam profile... 168 7e-42
At1g77220.1 68414.m08994 expressed protein contains Pfam profile... 154 9e-38
At4g38360.2 68417.m05424 expressed protein contains Pfam profile... 136 2e-32
At4g38360.1 68417.m05423 expressed protein contains Pfam profile... 136 2e-32
At1g11200.1 68414.m01283 expressed protein contains Pfam profile... 128 9e-30
At4g21570.1 68417.m03120 expressed protein contains Pfam profile... 123 2e-28
At1g23070.1 68414.m02884 hypothetical protein contains Pfam prof... 120 2e-27
At5g35180.1 68418.m04169 expressed protein 32 0.83
At3g61670.1 68416.m06911 expressed protein weak similarity to ex... 30 2.5
At2g46160.1 68415.m05740 zinc finger (C3HC4-type RING finger) fa... 30 3.3
At3g12685.1 68416.m01583 expressed protein contains Pfam domain,... 29 4.4
At2g40860.1 68415.m05044 protein kinase family protein / protein... 29 7.7
At2g40140.1 68415.m04937 zinc finger (CCCH-type) family protein ... 29 7.7
At1g61215.1 68414.m06898 DNA-binding bromodomain-containing prot... 29 7.7
>At5g26740.2 68418.m03164 expressed protein contains Pfam profile
PF03619: Domain of unknown function
Length = 422
Score = 171 bits (416), Expect = 7e-43
Identities = 77/215 (35%), Positives = 132/215 (61%), Gaps = 1/215 (0%)
Query: 65 ISIWQITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLRECYEAYV 124
++I+ I +H+++YT+P+ Q++I+RI++MVP+YA + + L P+ SIY DS+RE YEA+V
Sbjct: 23 LAIFHIYRHLLNYTEPTYQRYIVRIIFMVPVYAFMSFLSLVLPKSSIYFDSIREVYEAWV 82
Query: 125 IYNFMKYLLNYLNDGHDLEAVLETKP-QVYHIFPLCCLAPWEMGREFVHNCKHGILQYTL 183
IYNF+ L ++ + L + + CC P + F+ CK G LQ+ +
Sbjct: 83 IYNFLSLCLAWVGGPGSVVLSLSGRSLKPSWSLMTCCFPPLTLDGRFIRRCKQGCLQFVI 142
Query: 184 VRPITTVISMICELNDVYGESDFKPNVAFPYIIAINNLSQFVAMYCLVLFYKANRTELKP 243
++PI ++++ Y + +F P+ A+ Y+ I +S VA+Y LVLFY A R L+P
Sbjct: 143 LKPILVAVTLVLYAKGKYKDGNFNPDQAYLYLTIIYTISYTVALYALVLFYMACRDLLQP 202
Query: 244 MKPIGKFLCIKAVVFFSFFQGVIITILVYCGVLKT 278
P+ KF+ IK+VVF +++QGV++ + G +K+
Sbjct: 203 FNPVPKFVIIKSVVFLTYWQGVLVFLAAKSGFIKS 237
>At5g26740.1 68418.m03163 expressed protein contains Pfam profile
PF03619: Domain of unknown function
Length = 422
Score = 171 bits (416), Expect = 7e-43
Identities = 77/215 (35%), Positives = 132/215 (61%), Gaps = 1/215 (0%)
Query: 65 ISIWQITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLRECYEAYV 124
++I+ I +H+++YT+P+ Q++I+RI++MVP+YA + + L P+ SIY DS+RE YEA+V
Sbjct: 23 LAIFHIYRHLLNYTEPTYQRYIVRIIFMVPVYAFMSFLSLVLPKSSIYFDSIREVYEAWV 82
Query: 125 IYNFMKYLLNYLNDGHDLEAVLETKP-QVYHIFPLCCLAPWEMGREFVHNCKHGILQYTL 183
IYNF+ L ++ + L + + CC P + F+ CK G LQ+ +
Sbjct: 83 IYNFLSLCLAWVGGPGSVVLSLSGRSLKPSWSLMTCCFPPLTLDGRFIRRCKQGCLQFVI 142
Query: 184 VRPITTVISMICELNDVYGESDFKPNVAFPYIIAINNLSQFVAMYCLVLFYKANRTELKP 243
++PI ++++ Y + +F P+ A+ Y+ I +S VA+Y LVLFY A R L+P
Sbjct: 143 LKPILVAVTLVLYAKGKYKDGNFNPDQAYLYLTIIYTISYTVALYALVLFYMACRDLLQP 202
Query: 244 MKPIGKFLCIKAVVFFSFFQGVIITILVYCGVLKT 278
P+ KF+ IK+VVF +++QGV++ + G +K+
Sbjct: 203 FNPVPKFVIIKSVVFLTYWQGVLVFLAAKSGFIKS 237
>At3g05940.1 68416.m00676 expressed protein contains Pfam profile
PF03619: Domain of unknown function
Length = 422
Score = 168 bits (408), Expect = 7e-42
Identities = 76/216 (35%), Positives = 135/216 (62%), Gaps = 5/216 (2%)
Query: 65 ISIWQITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLRECYEAYV 124
++++ I +H+++YT+P Q++I+RI++MVP+YAL + + L P+ SIY +S+RE YEA+V
Sbjct: 23 LALFHIYKHLLNYTEPIYQRYIVRIVFMVPVYALMSFLALVLPKSSIYFNSIREVYEAWV 82
Query: 125 IYNFMKYLLNYLNDGHDLEAVL---ETKPQVYHIFPLCCLAPWEMGREFVHNCKHGILQY 181
IYNF+ L ++ + L KP +H+ CC+ P + F+ CK G LQ+
Sbjct: 83 IYNFLSLCLAWVGGPGSVVISLTGRSLKPS-WHLMT-CCIPPLPLDGRFIRRCKQGCLQF 140
Query: 182 TLVRPITTVISMICELNDVYGESDFKPNVAFPYIIAINNLSQFVAMYCLVLFYKANRTEL 241
+++PI ++++ Y + +F P+ ++ Y+ I +S VA+Y LVLFY A + L
Sbjct: 141 VILKPILVAVTLVLYAKGKYKDGNFSPDQSYLYLTIIYTISYTVALYALVLFYVACKDLL 200
Query: 242 KPMKPIGKFLCIKAVVFFSFFQGVIITILVYCGVLK 277
+P P+ KF+ IK+VVF +++QGV++ + G ++
Sbjct: 201 QPFNPVPKFVIIKSVVFLTYWQGVLVFLFAKSGFIR 236
>At1g77220.1 68414.m08994 expressed protein contains Pfam profile
PF03619: Domain of unknown function
Length = 484
Score = 154 bits (374), Expect = 9e-38
Identities = 80/231 (34%), Positives = 134/231 (58%), Gaps = 19/231 (8%)
Query: 65 ISIWQITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLRECYEAYV 124
+ ++ I +H+ Y +P QK +I ++ MVP+YA+ + + L E + + +R+CYEA+
Sbjct: 56 LPMYLIFEHLASYNQPEEQKFLIGLILMVPVYAVESFLSLVNSEAAFNCEVIRDCYEAFA 115
Query: 125 IYNFMKYLLNYLNDGHD-----LE---AVLETKPQ---------VYHIFPL-CCLAPWEM 166
+Y F +YL+ L DG + +E + ++ P V H FP+ C + W +
Sbjct: 116 LYCFERYLIACL-DGEERTIEFMEQQTVITQSTPLLEGTCSYGVVEHPFPMNCFVKDWSL 174
Query: 167 GREFVHNCKHGILQYTLVRPITTVISMICELNDVYGESDFKPNVAFPYIIAINNLSQFVA 226
G +F H K GI+QY +++ I +++MI E VYGE F N +PY+ + N SQ A
Sbjct: 175 GPQFYHAVKIGIVQYMILKMICALLAMILEAFGVYGEGKFAWNYGYPYLAVVLNFSQTWA 234
Query: 227 MYCLVLFYKANRTELKPMKPIGKFLCIKAVVFFSFFQGVIITILVYCGVLK 277
+YCLV FY + +L P+KP+ KFL K++VF +++QG+I+ L G++K
Sbjct: 235 LYCLVQFYNVIKDKLAPIKPLAKFLTFKSIVFLTWWQGIIVAFLFSMGLVK 285
>At4g38360.2 68417.m05424 expressed protein contains Pfam profile
PF03619: Domain of unknown function
Length = 485
Score = 136 bits (330), Expect = 2e-32
Identities = 75/231 (32%), Positives = 127/231 (54%), Gaps = 17/231 (7%)
Query: 65 ISIWQITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLRECYEAYV 124
+S++ + H+ Y P QK +I ++ MVP Y++ + L P S+ LR+CYE++
Sbjct: 34 LSLFLVFDHLSTYKNPEEQKFLIGVILMVPCYSIESFASLVKPSISVDCGILRDCYESFA 93
Query: 125 IYNFMKYLLNYLN-DGHDLE------------AVLETKPQ---VYHIFPL-CCLAPWEMG 167
+Y F +YL+ + + +E +L+ K + + H FP+ L PW +
Sbjct: 94 MYCFGRYLVACIGGEERTIEFMERQGRKSFKTPLLDHKDEKGIIKHPFPMNLFLKPWRLS 153
Query: 168 REFVHNCKHGILQYTLVRPITTVISMICELNDVYGESDFKPNVAFPYIIAINNLSQFVAM 227
F K GI+QY +++ +T + ++I E VY E +FK +PY+ + N SQ A+
Sbjct: 154 PWFYQVVKFGIVQYMIIKSLTALTALILEAFGVYCEGEFKWGCGYPYLAVVLNFSQSWAL 213
Query: 228 YCLVLFYKANRTELKPMKPIGKFLCIKAVVFFSFFQGVIITILVYCGVLKT 278
YCLV FY A + EL ++P+ KFL K++VF +++QGV I +L G+ K+
Sbjct: 214 YCLVQFYGATKDELAHIQPLAKFLTFKSIVFLTWWQGVAIALLSSLGLFKS 264
>At4g38360.1 68417.m05423 expressed protein contains Pfam profile
PF03619: Domain of unknown function
Length = 304
Score = 136 bits (330), Expect = 2e-32
Identities = 75/231 (32%), Positives = 127/231 (54%), Gaps = 17/231 (7%)
Query: 65 ISIWQITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLRECYEAYV 124
+S++ + H+ Y P QK +I ++ MVP Y++ + L P S+ LR+CYE++
Sbjct: 34 LSLFLVFDHLSTYKNPEEQKFLIGVILMVPCYSIESFASLVKPSISVDCGILRDCYESFA 93
Query: 125 IYNFMKYLLNYLN-DGHDLE------------AVLETKPQ---VYHIFPL-CCLAPWEMG 167
+Y F +YL+ + + +E +L+ K + + H FP+ L PW +
Sbjct: 94 MYCFGRYLVACIGGEERTIEFMERQGRKSFKTPLLDHKDEKGIIKHPFPMNLFLKPWRLS 153
Query: 168 REFVHNCKHGILQYTLVRPITTVISMICELNDVYGESDFKPNVAFPYIIAINNLSQFVAM 227
F K GI+QY +++ +T + ++I E VY E +FK +PY+ + N SQ A+
Sbjct: 154 PWFYQVVKFGIVQYMIIKSLTALTALILEAFGVYCEGEFKWGCGYPYLAVVLNFSQSWAL 213
Query: 228 YCLVLFYKANRTELKPMKPIGKFLCIKAVVFFSFFQGVIITILVYCGVLKT 278
YCLV FY A + EL ++P+ KFL K++VF +++QGV I +L G+ K+
Sbjct: 214 YCLVQFYGATKDELAHIQPLAKFLTFKSIVFLTWWQGVAIALLSSLGLFKS 264
>At1g11200.1 68414.m01283 expressed protein contains Pfam profile
PF03619: Domain of unknown function
Length = 295
Score = 128 bits (308), Expect = 9e-30
Identities = 67/217 (30%), Positives = 125/217 (57%), Gaps = 14/217 (6%)
Query: 70 ITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQS----IYLDSLRECYEAYVI 125
++QH+ ++ KP+ Q+ I+ I+ M P+YA+N+ +GL + S ++LD+++ECYEA VI
Sbjct: 32 VSQHLFYWKKPNEQRAILIIVLMAPVYAINSFVGLLDAKGSKPFFMFLDAVKECYEALVI 91
Query: 126 YNFMKYLLNYLNDGHDLEAVLETKP--QVYHIFPLCCLAPWEMGREFV--HNCKHGILQY 181
F+ + +Y+N + + +++H FP+ P +++ K Q+
Sbjct: 92 AKFLALMYSYVNISMSARIIPDEFKGREIHHSFPMTLFVPRTTHLDYLTLKQLKQWTWQF 151
Query: 182 TLVRPITTVISMICELNDVYGESDFKPNVAFPYIIAINNLSQFVAMYCLVLFYKANRTEL 241
++RP+ +++ + ++ +Y P AI N+S +A+Y LV FY EL
Sbjct: 152 CIIRPVCSILMITLQILGIY------PVWLSWIFTAILNVSVSLALYSLVKFYHVFAKEL 205
Query: 242 KPMKPIGKFLCIKAVVFFSFFQGVIITILVYCGVLKT 278
+P KP+ KF+C+K +VFF F+QG+++ ILV G++K+
Sbjct: 206 EPHKPLTKFMCVKGIVFFCFWQGIVLKILVGLGLIKS 242
>At4g21570.1 68417.m03120 expressed protein contains Pfam profile
PF03619: Domain of unknown function
Length = 294
Score = 123 bits (297), Expect = 2e-28
Identities = 72/221 (32%), Positives = 122/221 (55%), Gaps = 14/221 (6%)
Query: 66 SIWQITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQS----IYLDSLRECYE 121
+I ++QH+ H+ P QK I+ I+ M PIYA+ + IGL + S ++L+S++ECYE
Sbjct: 28 TIQLVSQHLFHWKNPKEQKAILIIVLMAPIYAVVSFIGLLEVKGSETFFLFLESIKECYE 87
Query: 122 AYVIYNFMKYLLNYLNDGHDLEAVLE--TKPQVYHIFPLCCLAPW--EMGREFVHNCKHG 177
A VI F+ + +YLN + + +++H FP+ P + R + K+
Sbjct: 88 ALVIAKFLALMYSYLNISMSKNILPDGIKGREIHHSFPMTLFQPHVVRLDRHTLKLLKYW 147
Query: 178 ILQYTLVRPITTVISMICELNDVYGESDFKPNVAFPYIIAINNLSQFVAMYCLVLFYKAN 237
Q+ ++RP+ + + + +L Y +++ + I +N S +A+Y LV+FY
Sbjct: 148 TWQFVVIRPVCSTLMIALQLIGFYPSW-----LSWTFTIIVN-FSVSLALYSLVIFYHVF 201
Query: 238 RTELKPMKPIGKFLCIKAVVFFSFFQGVIITILVYCGVLKT 278
EL P P+ KFLCIK +VFF F+QG+ + ILV G +K+
Sbjct: 202 AKELAPHNPLAKFLCIKGIVFFVFWQGIALDILVAMGFIKS 242
>At1g23070.1 68414.m02884 hypothetical protein contains Pfam profile
PF03619: Domain of unknown function
Length = 414
Score = 120 bits (289), Expect = 2e-27
Identities = 74/238 (31%), Positives = 123/238 (51%), Gaps = 26/238 (10%)
Query: 65 ISIWQITQHIVHYTKPSLQKHIIRILWMVPIYALNALIGLEFPEQSIYLDSLR---ECYE 121
+S++ I QH+ YT P+ QK I+ +L+MVP+YA ++I L + S+ D LR E +
Sbjct: 30 LSLYSILQHLRFYTNPAEQKWIVSVLFMVPVYATESIISLSNSKFSLPCDILRNCYEAFA 89
Query: 122 AYVIYNFMKYLLNYLN-----------------DGHDLEAVLET------KPQVYHIFPL 158
Y +++ L L+ + + +LE K + + F
Sbjct: 90 LYSFGSYLVACLGELSKIPNEWSGGERRVVEYLENESKKPLLEEGANESKKKKKKNSFWK 149
Query: 159 CCLAPWEMGREFVHNCKHGILQYTLVRPITTVISMICELNDVYGESDFKPNVAFPYIIAI 218
P+ +GRE K G++QY +++ ++ + EL VYG+ +FK +PYI+ +
Sbjct: 150 FLCDPYVLGRELFVIEKFGLVQYMILKTFCAFLTFLLELLGVYGDGEFKWYYGYPYIVVV 209
Query: 219 NNLSQFVAMYCLVLFYKANRTELKPMKPIGKFLCIKAVVFFSFFQGVIITILVYCGVL 276
N SQ A++CLV FY LK +KP+ KF+ KA+VF +++QG I +L Y G+L
Sbjct: 210 LNFSQMWALFCLVQFYNVTHERLKEIKPLAKFISFKAIVFATWWQGFGIALLCYYGIL 267
>At5g35180.1 68418.m04169 expressed protein
Length = 778
Score = 31.9 bits (69), Expect = 0.83
Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Query: 207 KPNVAFPYIIAINNLSQFVAMYCLVLFYKANRTELKPMKPIGKFL 251
+P P+I+ IN YCLVL+Y A+R + +GKF+
Sbjct: 620 EPGKKLPFILVINLQVPAKPNYCLVLYYAADR-PVNKTSSLGKFV 663
>At3g61670.1 68416.m06911 expressed protein weak similarity to
extra-large G-protein [Arabidopsis thaliana] GI:3201682
Length = 790
Score = 30.3 bits (65), Expect = 2.5
Identities = 17/48 (35%), Positives = 29/48 (60%), Gaps = 5/48 (10%)
Query: 372 TTETETPSVEVRPSAYGSIENTVTNMSMTSETEPLLDASDKSNDNENP 419
T E E SV V+ S+E+T +S +S + +LD+S+ S+D++ P
Sbjct: 41 TKEREADSVSVK-----SVEDTAKPVSASSPEKAILDSSETSSDSDVP 83
>At2g46160.1 68415.m05740 zinc finger (C3HC4-type RING finger)
family protein similar to RING-H2 zinc finger protein
ATL6 [Arabidopsis thaliana] GI:4928403; contains Pfam
profile PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 214
Score = 29.9 bits (64), Expect = 3.3
Identities = 15/54 (27%), Positives = 24/54 (44%)
Query: 145 VLETKPQVYHIFPLCCLAPWEMGREFVHNCKHGILQYTLVRPITTVISMICELN 198
+L P+ H F LCCL W C++ L P++T +S + L+
Sbjct: 150 MLRMMPECKHYFHLCCLDAWLKLNGSCPVCRNSPLPTPTSTPLSTPLSEVVPLS 203
>At3g12685.1 68416.m01583 expressed protein contains Pfam domain,
PF02681: Uncharacterized BCR, COG1963
Length = 213
Score = 29.5 bits (63), Expect = 4.4
Identities = 18/70 (25%), Positives = 30/70 (42%), Gaps = 2/70 (2%)
Query: 313 LAMWDVSDVKRDISEHLGVVGSSFSRRLRGKSMYHMTRGYDESSRLMGEPVPSSSAPNLT 372
L M+D V+R++ +H V+ + R + M +G + + L E + AP L
Sbjct: 128 LIMYDAQGVRREVGKHAKVLNKLTANARRSEVM--SLKGNESNKALTSEEISEEIAPPLK 185
Query: 373 TETETPSVEV 382
VEV
Sbjct: 186 ESIGHTEVEV 195
>At2g40860.1 68415.m05044 protein kinase family protein / protein
phosphatase 2C ( PP2C) family protein contains Pfam
PF00481: Protein phosphatase 2C domain; contains Pfam
PF00069: Protein kinase domain; similar to partner of
PIX 1 (GI:21702695) [Homo sapiens]
Length = 658
Score = 28.7 bits (61), Expect = 7.7
Identities = 21/76 (27%), Positives = 40/76 (52%), Gaps = 5/76 (6%)
Query: 278 TIFDISDTD---TVKIISSKLQPYVSPLDAS-PSCLGSFLA-MWDVSDVKRDISEHLGVV 332
T+ +++ T+ TV I+SS L+P ++ + P L S + W+ KR S+++ +
Sbjct: 251 TVLEMNYTEQQLTVAIVSSGLRPALAEIGLHLPKSLLSLIQNCWESDPSKRPSSDNVVLE 310
Query: 333 GSSFSRRLRGKSMYHM 348
S ++RGK H+
Sbjct: 311 LESIWEQVRGKQQGHL 326
>At2g40140.1 68415.m04937 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar) and Pfam domain,
PF00023: Ankyrin repeat
Length = 597
Score = 28.7 bits (61), Expect = 7.7
Identities = 22/60 (36%), Positives = 31/60 (51%), Gaps = 9/60 (15%)
Query: 304 ASPSCLGSFLAMWDVSDVKRDISEHLGV----VGSSFSRRLRGKSMYHMTRGYDESSRLM 359
AS +CL F A D+S KR+I E+ V G + RR+ K M G++E + LM
Sbjct: 28 ASATCLLEFAACDDLSSFKREIEENPSVEIDESGFWYCRRVGSKKM-----GFEERTPLM 82
>At1g61215.1 68414.m06898 DNA-binding bromodomain-containing protein
contains Pfam profile PF00439: Bromodomain
Length = 475
Score = 28.7 bits (61), Expect = 7.7
Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 1/65 (1%)
Query: 352 YDESSRLMGEPVPSSSAPNLTTETETPSVEVRPSAYGSIENTVTNMSMTSETE-PLLDAS 410
YD S L EP P S T +T ++ E T TN S +++E P++
Sbjct: 118 YDSSRTLSLEPSPKSEGGGECTSKDTSKDLSSVGSFTQQELTTTNWSPEAKSEAPVVIEQ 177
Query: 411 DKSND 415
+K+ D
Sbjct: 178 EKTKD 182
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.322 0.137 0.420
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,487,069
Number of Sequences: 28952
Number of extensions: 386209
Number of successful extensions: 980
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 959
Number of HSP's gapped (non-prelim): 20
length of query: 421
length of database: 12,070,560
effective HSP length: 83
effective length of query: 338
effective length of database: 9,667,544
effective search space: 3267629872
effective search space used: 3267629872
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 61 (28.7 bits)
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