BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001013-TA|BGIBMGA001013-PA|IPR002290|Serine/threonine protein kinase, IPR000719|Protein kinase, IPR008271|Serine/threonine protein kinase, active site, IPR001245|Tyrosine protein kinase, IPR011009|Protein kinase-like (360 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 89 7e-20 AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 88 1e-19 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 83 3e-18 AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 71 2e-14 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 64 2e-12 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 56 5e-10 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 56 5e-10 DQ667184-1|ABG75736.1| 489|Apis mellifera GABA-gated ion channe... 22 9.4 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 88.6 bits (210), Expect = 7e-20 Identities = 55/208 (26%), Positives = 97/208 (46%), Gaps = 6/208 (2%) Query: 30 IGEGSYAKVYKATHMVDETRHSIMACKVIDTAKAPRDYLTKFLPRELDVLIRINHPHIVH 89 +G G + +V D +R A K + A+ + + E ++ + +V Sbjct: 373 LGVGGFGRVELVQIAGDSSRS--FALKQMKKAQIVETRQQQHIMSEKRIMGEADCDFVVK 430 Query: 90 VSNIFQRRAKYFIFLRFAENGDLLDFLTQNGAIPENQSRLWMRQIISGLQYIHTMNIAHR 149 + F+ R ++ + G+L L G + +R + ++ Y+H+ NI +R Sbjct: 431 LFKTFKDRKYLYMLMEACLGGELWTVLRDKGHFDDGTTRFYTACVVEAFDYLHSRNIIYR 490 Query: 150 DLKCENVLVTANYNVKITDFGFARNVRQRDRDVLSETYCGSLSYAAPEVLKGVPYMPKLA 209 DLK EN+L+ + VK+ DFGFA+ + D + T+CG+ Y APEV+ + A Sbjct: 491 DLKPENLLLDSQGYVKLVDFGFAKRL---DHGRKTWTFCGTPEYVAPEVILNKGH-DISA 546 Query: 210 DMWSIGVILYTMLNKALPFNETSVKKLY 237 D WS+GV+++ +L PF K Y Sbjct: 547 DYWSLGVLMFELLTGTPPFTGGDPMKTY 574 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 88.2 bits (209), Expect = 1e-19 Identities = 53/175 (30%), Positives = 88/175 (50%), Gaps = 6/175 (3%) Query: 131 MRQIISGLQYIHTMNIAHRDLKCENVLVTANYN---VKITDFGFARNVRQRDRDVLSETY 187 ++QI+ + + H + HRDLK EN+L+ + VK+ DFG A V+ + + Sbjct: 15 IQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGEAQAWFG--F 72 Query: 188 CGSLSYAAPEVLKGVPYMPKLADMWSIGVILYTMLNKALPFNETSVKKLYEKQVMRKWRF 247 G+ Y +PEVLK PY K D+W+ GVILY +L PF + +LY + + + Sbjct: 73 AGTPGYLSPEVLKKEPY-GKPVDIWACGVILYILLVGYPPFWDEDQHRLYAQIKTGSYDY 131 Query: 248 RTTVVNQLSTECKEQVTQLMEPEPKSRPTASAVFNGTWIGMDSRLAKLTFLEESL 302 + + ++ E K + Q++ P R TAS WI R+A + +E++ Sbjct: 132 PSPEWDTVTPEAKNLINQMLTVNPSKRITASEALKHPWICQRERVASVVHRQETV 186 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 83.4 bits (197), Expect = 3e-18 Identities = 62/212 (29%), Positives = 100/212 (47%), Gaps = 16/212 (7%) Query: 21 EKGFI-LEKVIGEGSYAKVYKAT-HMVDETRHSI-MACKVIDTA---KAPRDYLTKFLPR 74 + G+I +E +IG G + V + + + R I +A K + KA D+LT Sbjct: 629 DAGYITIEAIIGGGEFGDVCRGKLKLPPDGRTEIDVAIKTLKPGSADKARNDFLT----- 683 Query: 75 ELDVLIRINHPHIVHVSNIFQRRAKYFIFLRFAENGDLLDFLTQN-GAIPENQSRLWMRQ 133 E ++ + HP+++ + + + I F ENG L FL N G Q +R Sbjct: 684 EASIMGQFEHPNVIFLQGVVTKSNPVMIITEFMENGSLDTFLRANDGKFQVLQLVGMLRG 743 Query: 134 IISGLQYIHTMNIAHRDLKCENVLVTANYNVKITDFGFARNVRQRDRDVLSETYCGSL-- 191 I SG+QY+ MN HRDL NVLV A KI DFG +R + + T G + Sbjct: 744 IASGMQYLAEMNYVHRDLAARNVLVNAALVCKIADFGLSREIESATEGAYT-TRGGKIPV 802 Query: 192 SYAAPEVLKGVPYMPKLADMWSIGVILYTMLN 223 + APE + +D+WS+G++ + +++ Sbjct: 803 RWTAPEAI-AFRKFTSASDVWSMGIVCWEVMS 833 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 70.9 bits (166), Expect = 2e-14 Identities = 56/199 (28%), Positives = 94/199 (47%), Gaps = 17/199 (8%) Query: 30 IGEGSYAKVYKATHMVDETRHSIMACKVIDTAKAPRDYLTKFLPRELDVLIRINHPHIVH 89 +G G + VYKA + ++ +A K+I T K + L E + H +IV Sbjct: 73 LGSGGFGIVYKALYKGEQ-----VAAKIIQTEK-----YSNMLNSEKHASF-LKHSNIVK 121 Query: 90 VSNIFQRRAKYFIFLRFAENGDLLDFLTQNGAIPENQSRLWMRQIISGLQYIHTMNIAHR 149 V I Q + I + G L + +N+ ++ I LQ+ H I H Sbjct: 122 VLMIEQGASLSLITMELC--GTTLQNRLDEAILIKNERICILKSITCALQFCHNAGIVHA 179 Query: 150 DLKCENVLVTANYNVKITDFGFARNVRQRDRDVLSETYCGSLSYAAPEVLKGVPYMPKLA 209 D+K +N+L++ N K+TDFG + + + + + G+ Y APEV+K P A Sbjct: 180 DVKPKNILMSKNGQPKLTDFGSSVLIGAPNE---IDKFYGTPGYTAPEVIKQNRPTP-AA 235 Query: 210 DMWSIGVILYTMLNKALPF 228 D++S+G++ + ML + LPF Sbjct: 236 DIYSLGIVAWQMLFRKLPF 254 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 63.7 bits (148), Expect = 2e-12 Identities = 31/106 (29%), Positives = 57/106 (53%), Gaps = 2/106 (1%) Query: 85 PHIVHVSNIFQRRAKYFIFLRFAENGDLLDFLTQNGAIPENQSRLWMRQIISGLQYIHTM 144 P +V + + FQ + + + + GDL+ + Q G E + + +I GL ++H Sbjct: 45 PFLVQLHSCFQTMDRLYFVMEYVNGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGR 104 Query: 145 NIAHRDLKCENVLVTANYNVKITDFGFARNVRQRDRDVLSETYCGS 190 I +RDLK +NVL+ + ++KI DFG + D+ ++T+CG+ Sbjct: 105 GIVYRDLKLDNVLLDQDGHIKIADFGMCKEGISGDK--TTKTFCGT 148 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 56.0 bits (129), Expect = 5e-10 Identities = 39/154 (25%), Positives = 72/154 (46%), Gaps = 14/154 (9%) Query: 134 IISGLQYIHTMNIAHRDLKCENVLVTANYNVKITDFGFARNVRQRDRDVLSETYCGSLSY 193 ++ G++Y+H+ + HRD+K +NVL+ K+TDFGF ++ + G+ + Sbjct: 706 VLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCIT-----EVMMLGSIVGTPVH 760 Query: 194 AAPEVLKGVPYMPKLADMWSIGVILYTML--NKALP--FNETSVKKLY---EKQVMRKWR 246 APE+L G + D+++ G++ + + + LP F + K+L K+ + Sbjct: 761 MAPELLSG--HYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWTSVKKALMIVG 818 Query: 247 FRTTVVNQLSTECKEQVTQLMEPEPKSRPTASAV 280 R + EC + Q EP RP A+ Sbjct: 819 IRPERLPSFDDECWRLMEQCWSGEPSKRPLLGAI 852 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 56.0 bits (129), Expect = 5e-10 Identities = 39/154 (25%), Positives = 72/154 (46%), Gaps = 14/154 (9%) Query: 134 IISGLQYIHTMNIAHRDLKCENVLVTANYNVKITDFGFARNVRQRDRDVLSETYCGSLSY 193 ++ G++Y+H+ + HRD+K +NVL+ K+TDFGF ++ + G+ + Sbjct: 744 VLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCIT-----EVMMLGSIVGTPVH 798 Query: 194 AAPEVLKGVPYMPKLADMWSIGVILYTML--NKALP--FNETSVKKLY---EKQVMRKWR 246 APE+L G + D+++ G++ + + + LP F + K+L K+ + Sbjct: 799 MAPELLSG--HYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWTSVKKALMIVG 856 Query: 247 FRTTVVNQLSTECKEQVTQLMEPEPKSRPTASAV 280 R + EC + Q EP RP A+ Sbjct: 857 IRPERLPSFDDECWRLMEQCWSGEPSKRPLLGAI 890 >DQ667184-1|ABG75736.1| 489|Apis mellifera GABA-gated ion channel protein. Length = 489 Score = 21.8 bits (44), Expect = 9.4 Identities = 8/15 (53%), Positives = 11/15 (73%) Query: 128 RLWMRQIISGLQYIH 142 R+W++QI LQ IH Sbjct: 4 RMWLQQIFILLQMIH 18 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.319 0.134 0.387 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 101,049 Number of Sequences: 429 Number of extensions: 4269 Number of successful extensions: 19 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 5 Number of HSP's gapped (non-prelim): 8 length of query: 360 length of database: 140,377 effective HSP length: 59 effective length of query: 301 effective length of database: 115,066 effective search space: 34634866 effective search space used: 34634866 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 44 (21.8 bits)
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