BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001012-TA|BGIBMGA001012-PA|IPR011009|Protein kinase-like, IPR000719|Protein kinase, IPR008271|Serine/threonine protein kinase, active site, IPR001245|Tyrosine protein kinase, IPR002290|Serine/threonine protein kinase (655 letters) Database: mosquito 2123 sequences; 516,269 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CR954256-9|CAJ14150.1| 872|Anopheles gambiae putative calcium/c... 95 4e-21 AJ301655-1|CAC35008.1| 1433|Anopheles gambiae putative epidermal... 68 9e-13 AY578807-1|AAT07312.1| 438|Anopheles gambiae punt protein. 58 6e-10 AY578811-1|AAT07316.1| 565|Anopheles gambiae thickveins protein. 55 5e-09 AY578798-1|AAT07303.1| 356|Anopheles gambiae baboon protein. 52 5e-08 AY578808-1|AAT07313.1| 458|Anopheles gambiae saxophone protein. 50 3e-07 AY578812-1|AAT07317.1| 932|Anopheles gambiae wishful thinking p... 43 3e-05 AJ441131-7|CAD29636.1| 1977|Anopheles gambiae putative Tyr/Ser/T... 27 2.1 AB090821-2|BAC57918.1| 1168|Anopheles gambiae reverse transcript... 26 2.7 AY753540-1|AAV28543.1| 3320|Anopheles gambiae SGS3 protein. 25 6.3 >CR954256-9|CAJ14150.1| 872|Anopheles gambiae putative calcium/calmodulin-dependentprotein kinase, CAKI protein. Length = 872 Score = 95.5 bits (227), Expect = 4e-21 Identities = 81/278 (29%), Positives = 125/278 (44%), Gaps = 15/278 (5%) Query: 354 ACKVIDTS--TAPRDFVKKFLPREIEMLIKLSHPHLVHTHSIFQRR-YKYFIFMRYMERG 410 A K++D + TA L RE + L HPH+V + Y +F ME Sbjct: 19 AVKIVDVAKFTASPGLSTSDLKREATICHMLKHPHIVELLETYSSEGMLYMVFD--MEGS 76 Query: 411 DLLEFVLQRGAV----QEDQARIWTRQLALAIQYMHELEIAHRDIKCENVLLTANQN--- 463 D+ V++R E A + RQ+ A++Y HE +I HRD++ LL N Sbjct: 77 DICFEVVRRAVAGFVYSEAVACHYLRQILEALRYCHENDIIHRDVRPACALLATADNSAP 136 Query: 464 VKLSDFGFARMCVDKKLNEIRSETFCGSLSYTAPEILQGTPYLPKPTDVWSLGIVVYVML 523 VKL FG A + + + + + G Y APE++ Y KP DVW G++++V+L Sbjct: 137 VKLGGFGSAVQLPNGR-DSVETHGRVGCPHYMAPEVVARRVY-GKPCDVWGAGVMLHVLL 194 Query: 524 NRAMPFEDKHIKQLHQAQINKNWKFRSRYIDTISDNCKRLIGLMLEPNHQNRLKIYDIVN 583 + +PF K+L A + IS N K L+ ML PN +R I ++++ Sbjct: 195 SGRLPFHGSG-KRLQDAIARGRVTLDTPEWKHISSNAKDLVLKMLAPNPISRPTITEVLD 253 Query: 584 SEWIAMDSRLLEWTPQETLAYKKAREEKNKLHKTVETV 621 WI +L +T+ K + KL V+TV Sbjct: 254 HPWIRDRDKLQRIHLGDTVEELKRYNARRKLKAAVQTV 291 Score = 92.3 bits (219), Expect = 4e-20 Identities = 76/270 (28%), Positives = 120/270 (44%), Gaps = 13/270 (4%) Query: 50 ACKIIETAK--APKDFVVKFLPREIDVLVRLNHPHLIHVHSIFQRKTKYYIFMRYTENGD 107 A KI++ AK A L RE + L HPH++ + + + Y+ E D Sbjct: 19 AVKIVDVAKFTASPGLSTSDLKREATICHMLKHPHIVELLETYSSEGMLYMVFDM-EGSD 77 Query: 108 LLSYILKNGCV----SENQARVWTRQLALGLQYLHELEIAHRDIKCENVLLTANFN---V 160 + +++ SE A + RQ+ L+Y HE +I HRD++ LL N V Sbjct: 78 ICFEVVRRAVAGFVYSEAVACHYLRQILEALRYCHENDIIHRDVRPACALLATADNSAPV 137 Query: 161 KLSDFGFSRFCVESDNQPVLSETYCGSMSYAAPEILRGKPYCPKPTDLWSLGVVLFVMLN 220 KL FG S + + V + G Y APE++ + Y KP D+W GV+L V+L+ Sbjct: 138 KLGGFG-SAVQLPNGRDSVETHGRVGCPHYMAPEVVARRVY-GKPCDVWGAGVMLHVLLS 195 Query: 221 KSMPFDDTRMRKLYEQQMGKKYRFRSRVASILSLECKTVVKHLLEPDPGLRHSATNVLDS 280 +PF + R L + + + +S K +V +L P+P R + T VLD Sbjct: 196 GRLPFHGSGKR-LQDAIARGRVTLDTPEWKHISSNAKDLVLKMLAPNPISRPTITEVLDH 254 Query: 281 EWIAMDSRLTTLNAVEAAALKRAKEDRRKL 310 WI +L ++ + + RRKL Sbjct: 255 PWIRDRDKLQRIHLGDTVEELKRYNARRKL 284 >AJ301655-1|CAC35008.1| 1433|Anopheles gambiae putative epidermal growth factor receptorprotein. Length = 1433 Score = 67.7 bits (158), Expect = 9e-13 Identities = 48/213 (22%), Positives = 108/213 (50%), Gaps = 7/213 (3%) Query: 25 VGEGAYAKVYLTEYSSKEDSHKSTLACKIIETAKAPKDFVVKFLPREIDVLVRLNHPHLI 84 +G GA+ +V+ + + +S K +A K++ + +FL E ++ + HP+L+ Sbjct: 840 LGMGAFGRVFKGVWMPEGESVKIPVAIKVLMEMSGSESSK-EFL-EEAYIMASVEHPNLL 897 Query: 85 HVHSIFQRKTKYYIFMRYTENGDLLSYILKNGCVSENQARV-WTRQLALGLQYLHELEIA 143 + ++ ++ + + G LL Y+ N ++A + W+ Q+A G+ YL E + Sbjct: 898 KLLAVCMT-SQMMLITQLMPLGCLLDYVRNNKDKIGSKALLNWSTQIARGMAYLEERRLV 956 Query: 144 HRDIKCENVLLTANFNVKLSDFGFSRFCVESDNQPVLSETYCGSMSYAAPEILRGKPYCP 203 HRD+ NVL+ VK++ FG ++ ++ D+ + + + A E +R + + Sbjct: 957 HRDLAARNVLVQTPSCVKITVFGLAKL-LDFDSDEYRAAGGKMPIKWLALECIRHRVFTS 1015 Query: 204 KPTDLWSLGVVLFVMLN-KSMPFDDTRMRKLYE 235 K +D+W+ G+ ++ +L + P+++ + + E Sbjct: 1016 K-SDVWAFGITIWELLTYGARPYENVPAKDVPE 1047 Score = 64.1 bits (149), Expect = 1e-11 Identities = 45/171 (26%), Positives = 87/171 (50%), Gaps = 6/171 (3%) Query: 369 KKFLPREIEMLIKLSHPHLVHTHSIFQRRYKYFIFMRYMERGDLLEFVLQRGAVQEDQAR 428 K+FL E ++ + HP+L+ ++ + + + M G LL++V +A Sbjct: 879 KEFL-EEAYIMASVEHPNLLKLLAVCMTS-QMMLITQLMPLGCLLDYVRNNKDKIGSKAL 936 Query: 429 I-WTRQLALAIQYMHELEIAHRDIKCENVLLTANQNVKLSDFGFARMCVDKKLNEIRSET 487 + W+ Q+A + Y+ E + HRD+ NVL+ VK++ FG A++ +D +E R+ Sbjct: 937 LNWSTQIARGMAYLEERRLVHRDLAARNVLVQTPSCVKITVFGLAKL-LDFDSDEYRAAG 995 Query: 488 FCGSLSYTAPEILQGTPYLPKPTDVWSLGIVVYVMLN-RAMPFEDKHIKQL 537 + + A E ++ + K +DVW+ GI ++ +L A P+E+ K + Sbjct: 996 GKMPIKWLALECIRHRVFTSK-SDVWAFGITIWELLTYGARPYENVPAKDV 1045 >AY578807-1|AAT07312.1| 438|Anopheles gambiae punt protein. Length = 438 Score = 58.4 bits (135), Expect = 6e-10 Identities = 54/195 (27%), Positives = 93/195 (47%), Gaps = 31/195 (15%) Query: 70 REIDVLVRLNHPHLIHVHSIFQRK----TKYYIFMRYTENGDLLSYILKNGCVSENQARV 125 ++I L R+NHP+++ +R T +++ Y ENG L + LK VS + Sbjct: 161 QDIFKLPRMNHPNILEFIGCEKRSDMASTDFWLITAYCENGSLCDF-LKAHTVSWTELCK 219 Query: 126 WTRQLALGLQYLHEL-----------EIAHRDIKCENVLLTANFNVKLSDFGFSRFCVES 174 +A GL +LHE IAHRD K +NVLL A+ ++DFG + Sbjct: 220 IATTMARGLTHLHEEIQSSRTDGLKPSIAHRDFKSKNVLLKADLTACIADFGLALVFTPG 279 Query: 175 DNQPVLSETY--CGSMSYAAPEILRGKPYCPKPT----DLWSLGVVLFVMLNKSM----P 224 + +T+ G+ Y APE+L G + D+++ G+VL+ ++++ P Sbjct: 280 KS---CGDTHGQVGTRRYMAPEVLEGAINFTRDAFLRIDVYACGLVLWELVSRCTVHGGP 336 Query: 225 FDDTRMRKLYEQQMG 239 D+ R+ +E ++G Sbjct: 337 VDEYRLP--FEAELG 349 Score = 51.2 bits (117), Expect = 8e-08 Identities = 43/172 (25%), Positives = 82/172 (47%), Gaps = 23/172 (13%) Query: 374 REIEMLIKLSHPHLVHTHSIFQRR----YKYFIFMRYMERGDLLEFVLQRGAVQEDQARI 429 ++I L +++HP+++ +R +++ Y E G L +F+ + +I Sbjct: 161 QDIFKLPRMNHPNILEFIGCEKRSDMASTDFWLITAYCENGSLCDFLKAHTVSWTELCKI 220 Query: 430 WTRQLALAIQYMHEL-----------EIAHRDIKCENVLLTANQNVKLSDFGFARMCV-D 477 T +A + ++HE IAHRD K +NVLL A+ ++DFG A + Sbjct: 221 ATT-MARGLTHLHEEIQSSRTDGLKPSIAHRDFKSKNVLLKADLTACIADFGLALVFTPG 279 Query: 478 KKLNEIRSETFCGSLSYTAPEILQGTPYLPKPT----DVWSLGIVVYVMLNR 525 K + + G+ Y APE+L+G + DV++ G+V++ +++R Sbjct: 280 KSCGDTHGQV--GTRRYMAPEVLEGAINFTRDAFLRIDVYACGLVLWELVSR 329 >AY578811-1|AAT07316.1| 565|Anopheles gambiae thickveins protein. Length = 565 Score = 55.2 bits (127), Expect = 5e-09 Identities = 40/142 (28%), Positives = 69/142 (48%), Gaps = 15/142 (10%) Query: 94 TKYYIFMRYTENGDLLSYILKNGCVSENQARVWTRQLALGLQYLH--------ELEIAHR 145 T+ + Y E G L Y+ K ++ + + LA G+ +LH + IAHR Sbjct: 328 TQMLLITDYHELGSLHDYLQKR-VLNPHMLKTLAHSLASGVAHLHTEIFGTPGKPSIAHR 386 Query: 146 DIKCENVLLTANFNVKLSDFGFS-RFCVESDNQPVLSETYCGSMSYAAPEILRGKPYCP- 203 DIK +N+L+ N ++DFG + ++ ESD + + + G+ Y APE+L Sbjct: 387 DIKSKNILVKRNGQCAIADFGLAVKYTSESDTIQIANNSRVGTRRYMAPEVLSETLDLNL 446 Query: 204 ----KPTDLWSLGVVLFVMLNK 221 K D++S+G+V + M + Sbjct: 447 FEGFKMADMYSVGLVFWEMARR 468 Score = 52.8 bits (121), Expect = 3e-08 Identities = 39/136 (28%), Positives = 66/136 (48%), Gaps = 15/136 (11%) Query: 406 YMERGDLLEFVLQRGAVQEDQARIWTRQLALAIQYMH--------ELEIAHRDIKCENVL 457 Y E G L ++ LQ+ + + LA + ++H + IAHRDIK +N+L Sbjct: 336 YHELGSLHDY-LQKRVLNPHMLKTLAHSLASGVAHLHTEIFGTPGKPSIAHRDIKSKNIL 394 Query: 458 LTANQNVKLSDFGFA-RMCVDKKLNEIRSETFCGSLSYTAPEILQGTPYLP-----KPTD 511 + N ++DFG A + + +I + + G+ Y APE+L T L K D Sbjct: 395 VKRNGQCAIADFGLAVKYTSESDTIQIANNSRVGTRRYMAPEVLSETLDLNLFEGFKMAD 454 Query: 512 VWSLGIVVYVMLNRAM 527 ++S+G+V + M R + Sbjct: 455 MYSVGLVFWEMARRCI 470 >AY578798-1|AAT07303.1| 356|Anopheles gambiae baboon protein. Length = 356 Score = 52.0 bits (119), Expect = 5e-08 Identities = 40/137 (29%), Positives = 64/137 (46%), Gaps = 15/137 (10%) Query: 94 TKYYIFMRYTENGDLLSYILKNGCVSENQARVWTRQLALGLQYLH--------ELEIAHR 145 T+ ++ Y ENG L ++ CV + +A GL +LH + IAHR Sbjct: 128 TQLWLVTDYHENGSLFDFLTAR-CVDPDTMLEMAFSIATGLAHLHMDIVGTRGKPAIAHR 186 Query: 146 DIKCENVLLTANFNVKLSDFGFS-RFCVESDNQPVLSETYCGSMSYAAPEILRGKPYCP- 203 D+K +N+L+ +N + D G + R V +D S G+ Y APE+L Sbjct: 187 DLKSKNILVKSNLTCCIGDLGLAVRHIVATDTVDQPSTHRVGTKRYMAPEVLDETINVSQ 246 Query: 204 ----KPTDLWSLGVVLF 216 K D+++LG+VL+ Sbjct: 247 FDSFKRADVYALGLVLW 263 Score = 46.8 bits (106), Expect = 2e-06 Identities = 39/134 (29%), Positives = 62/134 (46%), Gaps = 15/134 (11%) Query: 406 YMERGDLLEFVLQRGAVQEDQARIWTRQLALAIQYMH--------ELEIAHRDIKCENVL 457 Y E G L +F+ R V D +A + ++H + IAHRD+K +N+L Sbjct: 136 YHENGSLFDFLTAR-CVDPDTMLEMAFSIATGLAHLHMDIVGTRGKPAIAHRDLKSKNIL 194 Query: 458 LTANQNVKLSDFGFA-RMCVDKKLNEIRSETFCGSLSYTAPEILQGTPYLP-----KPTD 511 + +N + D G A R V + S G+ Y APE+L T + K D Sbjct: 195 VKSNLTCCIGDLGLAVRHIVATDTVDQPSTHRVGTKRYMAPEVLDETINVSQFDSFKRAD 254 Query: 512 VWSLGIVVYVMLNR 525 V++LG+V++ + R Sbjct: 255 VYALGLVLWEIARR 268 >AY578808-1|AAT07313.1| 458|Anopheles gambiae saxophone protein. Length = 458 Score = 49.6 bits (113), Expect = 3e-07 Identities = 33/112 (29%), Positives = 55/112 (49%), Gaps = 10/112 (8%) Query: 94 TKYYIFMRYTENGDLLSYILKNGCVSENQARVWTRQLALGLQYLH--------ELEIAHR 145 T+ ++ Y G L Y L +S +Q +A G+ +LH + IAHR Sbjct: 220 TQLWLITHYYPQGSLFDY-LNRTAISTHQMITICLSIANGMVHLHTEIFGTEGKPAIAHR 278 Query: 146 DIKCENVLLTANFNVKLSDFGFSRFCVESDNQPVLSET-YCGSMSYAAPEIL 196 D+K +N+L+ AN ++DFG + ++ N+ + T G+ Y APE+L Sbjct: 279 DLKTKNILIRANGTCVIADFGLAVMHSQTTNKIDIGNTARVGTKRYMAPEVL 330 Score = 45.6 bits (103), Expect = 4e-06 Identities = 34/141 (24%), Positives = 67/141 (47%), Gaps = 15/141 (10%) Query: 401 FIFMRYMERGDLLEFVLQRGAVQEDQARIWTRQLALAIQYMH--------ELEIAHRDIK 452 ++ Y +G L ++ L R A+ Q +A + ++H + IAHRD+K Sbjct: 223 WLITHYYPQGSLFDY-LNRTAISTHQMITICLSIANGMVHLHTEIFGTEGKPAIAHRDLK 281 Query: 453 CENVLLTANQNVKLSDFGFARM-CVDKKLNEIRSETFCGSLSYTAPEILQGTPYLP---- 507 +N+L+ AN ++DFG A M +I + G+ Y APE+L + + Sbjct: 282 TKNILIRANGTCVIADFGLAVMHSQTTNKIDIGNTARVGTKRYMAPEVLDESISMECFDA 341 Query: 508 -KPTDVWSLGIVVYVMLNRAM 527 + D++++G++ + + R + Sbjct: 342 LRKADIYAIGLIFWEVCRRTI 362 >AY578812-1|AAT07317.1| 932|Anopheles gambiae wishful thinking protein. Length = 932 Score = 42.7 bits (96), Expect = 3e-05 Identities = 29/98 (29%), Positives = 48/98 (48%), Gaps = 14/98 (14%) Query: 446 IAHRDIKCENVLLTANQNVKLSDFGFARMCVDKK--------LNEIRSETFCGSLSYTAP 497 I HRD+ N+L+ ++ + + D GFA + L E +S G++ Y AP Sbjct: 369 ICHRDLNSRNILVKSDLSCCIGDLGFALKTFGARYEYRGEITLAETKSINEVGTVRYMAP 428 Query: 498 EILQGTPYLP------KPTDVWSLGIVVYVMLNRAMPF 529 E+L+G L K DV++L +V++ + NR F Sbjct: 429 EVLEGAVNLRDCESALKQIDVYTLALVLWELANRCEDF 466 Score = 41.5 bits (93), Expect = 7e-05 Identities = 64/258 (24%), Positives = 114/258 (44%), Gaps = 44/258 (17%) Query: 20 KLTKFVGEGAYAKVYLTEYSSKEDSHKSTLACKIIETAKAPKDFVVKFLPREIDVLVRLN 79 KL +G+G Y V+ K ++ +A KI +A+ + F+ + R+I + + Sbjct: 244 KLVSMIGQGKYGTVW------KGIVNEKPVAVKIF-SAQHRQYFLNE---RDIYTVPLME 293 Query: 80 HPHLIHVHSIFQRKT-----KYYIFMRYTENGDLLSYILKNGCVSENQARVWTRQLALGL 134 P L+ +R+T +Y + + G L ++ N R+ + +A GL Sbjct: 294 SPSLLAYFGSDERRTLDDRIEYMLVLSLAPLGCLQDWLTDNSVPFSTFCRMG-KSIANGL 352 Query: 135 QYLH------ELE---IAHRDIKCENVLLTANFNVKLSDFGFSRFC----VESDNQPVLS 181 +LH EL I HRD+ N+L+ ++ + + D GF+ E + L+ Sbjct: 353 AHLHTEIRKGELVKPCICHRDLNSRNILVKSDLSCCIGDLGFALKTFGARYEYRGEITLA 412 Query: 182 ET----YCGSMSYAAPEILRGKPY---CP---KPTDLWSLGVVLFVMLNKSMPF--DDTR 229 ET G++ Y APE+L G C K D+++L +VL+ + N+ F + T Sbjct: 413 ETKSINEVGTVRYMAPEVLEGAVNLRDCESALKQIDVYTLALVLWELANRCEDFYPEGTT 472 Query: 230 M---RKLYEQQMGKKYRF 244 + R YE+ +G F Sbjct: 473 VPEYRAPYEEYVGSNPNF 490 >AJ441131-7|CAD29636.1| 1977|Anopheles gambiae putative Tyr/Ser/Thr phosphatase protein. Length = 1977 Score = 26.6 bits (56), Expect = 2.1 Identities = 13/36 (36%), Positives = 24/36 (66%), Gaps = 1/36 (2%) Query: 609 EEKNKLHKTVETVELPGSSKTEHKV-CLPDSLVENN 643 +E+N++ K E +PGSS T K CL ++++++N Sbjct: 1560 DEENEVEKDKEKEGMPGSSVTAAKERCLYEAVLKHN 1595 >AB090821-2|BAC57918.1| 1168|Anopheles gambiae reverse transcriptase protein. Length = 1168 Score = 26.2 bits (55), Expect = 2.7 Identities = 11/23 (47%), Positives = 13/23 (56%) Query: 149 CENVLLTANFNVKLSDFGFSRFC 171 C VLL +FN D+G SR C Sbjct: 114 CSRVLLAGDFNAMHLDWGSSRTC 136 >AY753540-1|AAV28543.1| 3320|Anopheles gambiae SGS3 protein. Length = 3320 Score = 25.0 bits (52), Expect = 6.3 Identities = 10/30 (33%), Positives = 16/30 (53%) Query: 433 QLALAIQYMHELEIAHRDIKCENVLLTANQ 462 Q L + + E E+ H + ++VLL NQ Sbjct: 11 QQLLNVSFGDEFEVFHTQVSAQHVLLVRNQ 40 Database: mosquito Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 516,269 Number of sequences in database: 2123 Lambda K H 0.320 0.135 0.400 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 681,272 Number of Sequences: 2123 Number of extensions: 27744 Number of successful extensions: 60 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 32 Number of HSP's gapped (non-prelim): 19 length of query: 655 length of database: 516,269 effective HSP length: 68 effective length of query: 587 effective length of database: 371,905 effective search space: 218308235 effective search space used: 218308235 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 51 (24.6 bits)
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