BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001011-TA|BGIBMGA001011-PA|IPR001382|Glycoside hydrolase, family 47 (686 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 324 2e-89 SPAC2E1P5.01c |mns1|SPAPB1E7.13c|mannosyl-oligosaccharide 1,2-al... 135 2e-32 SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 35 0.043 SPBC23G7.07c |||replication regulator |Schizosaccharomyces pombe... 31 0.40 SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 31 0.53 SPAC1751.03 ||SPAC31A2.01|translation initiation factor eIF3m|Sc... 31 0.70 SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual 30 1.2 SPCC962.02c |bir1|cut17, pbh1, SPCP31B10.10c|survivin homolog|Sc... 30 1.2 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 29 1.6 SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 29 2.8 SPBC216.01c ||SPBC713.13c|DNA damage response protein |Schizosac... 29 2.8 SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi... 29 2.8 SPCC63.08c |ppk36|atg1|serine/threonine protein kinase Ppk36|Sch... 28 4.9 SPBP8B7.28c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 28 4.9 SPCC965.05c |thp1||uracil DNA N-glycosylase Thp1 |Schizosaccharo... 28 4.9 SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1... 28 4.9 SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 28 4.9 SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyc... 27 6.5 SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 27 8.6 SPBC21H7.02 |taf10||transcription factor TFIID complex subunit T... 27 8.6 >SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 787 Score = 324 bits (797), Expect = 2e-89 Identities = 175/424 (41%), Positives = 255/424 (60%), Gaps = 22/424 (5%) Query: 1 MFQHAYDSYLRYAYPYDELRPLSCDGV-------------DTWGSYSLTLIDALDTLAIM 47 +F H Y++Y+++A+P DEL PLSC+G+ D G Y LTL+D LDTL ++ Sbjct: 42 LFYHGYNNYMQFAFPNDELAPLSCEGLGPDYENPNNIGVNDVRGDYLLTLVDVLDTLVVL 101 Query: 48 GNYSEFNRVVDIVLQKRHFDADINVSVFETNIRIVGGLLSAHLLS--HKTGMKLEPGWPC 105 G+ F VD V+ +F+ D V VFE IRI+GGLLS+H+ + K G ++ P + Sbjct: 102 GDREGFQDAVDKVIHHINFERDTKVQVFEATIRILGGLLSSHIFASEEKYGFQI-PLY-- 158 Query: 106 NGPLLRLAEDVAQRLIAAFDTTTGMPYGTINLRSGVPPGETSITCTAGVGTFIIEFGTLS 165 G LL LA ++A+RL+ AF T TG+P+ INL GV E + C A + ++EF L+ Sbjct: 159 KGELLTLATELAERLLPAFRTPTGIPFARINLMKGVAYREVTENCAAAASSLVLEFSMLT 218 Query: 166 RLTGDPLYEEVAYNALKALYHHRSPIGLLGNHIDVMTGRWTAQDAGIGGGIDSYYEYLVK 225 LTG+ ++ A NA +++ RS IGLLGN IDV++GRW +G+G GIDS+YEY K Sbjct: 219 ALTGNNKFKASAENAFFSVWKRRSGIGLLGNSIDVLSGRWIYPVSGVGAGIDSFYEYAFK 278 Query: 226 GAILLEKPELMSMFLEARQSIEKYLKKDDWFVWATM--LRGHVTLPVFQSLESYWPGLLS 283 I L P + ++ ++ +S+ Y + + + + G V SL +Y+PGLL Sbjct: 279 SYIFLGDPRYLEVWQKSLESLRHYTASLNEYYYQNVYSANGMVMSRWVDSLSAYFPGLLV 338 Query: 284 LIGESDAAMRIIHNYHSVWRQYGFTPEVYNLGTGEASSSRESYPLRPELIESIMYLYRDT 343 L GE + A ++ Y S++ ++G PE YNL T S YPLRPE ES YLYR T Sbjct: 339 LAGELELAKKMHLYYFSIYLKFGQLPERYNLYT--KSIELNGYPLRPEFAESTYYLYRAT 396 Query: 344 RDPILLQMGEDILRSIQHSARTPCGYATIKDVRDHRKEDRMESFFLAETTKYLYLLFDPD 403 +D L +GE +L +I++ TPCG+A ++++ + +RMESFFL+ET KYL+LLFD D Sbjct: 397 KDVFYLHVGELLLSNIENHLWTPCGFAAVENLEKYTLSNRMESFFLSETLKYLFLLFDDD 456 Query: 404 NFIH 407 N IH Sbjct: 457 NPIH 460 >SPAC2E1P5.01c |mns1|SPAPB1E7.13c|mannosyl-oligosaccharide 1,2-alpha-mannosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 135 bits (327), Expect = 2e-32 Identities = 155/490 (31%), Positives = 219/490 (44%), Gaps = 79/490 (16%) Query: 2 FQHAYDSYLRYAYPYDELRPLSCDGVDTWGS-YSLTLIDALDTLAIMGNYSEFNRVVDIV 60 F ++ Y Y + DE P+ G + +ID+LDT+ IMG + R + V Sbjct: 54 FIESWTDYETYGWGKDEYYPIIKRGRNYLRKGMGWMIIDSLDTMMIMGLDEQVLRAREWV 113 Query: 61 LQKRHFDADIN-VSVFETNIRIVGGLLSAHLLSHKTGMKLEPGWPCNGPLLRLAEDVAQR 119 ++ D VSVFET IRI+GGLLS++ LS KL L A D+A R Sbjct: 114 NNSLTWNQDDEEVSVFETTIRILGGLLSSYHLSQD---KL---------YLDRAVDLADR 161 Query: 120 LIAAFDTTTGMPYGTINL--RSGVPPGETSITCTAGVGTFIIEFGTLSRLTGDPLYEEVA 177 L+AA++T+TG+P +NL R TA GT +E LS LTGDP Y A Sbjct: 162 LLAAYNTSTGLPRSNVNLGTRKSRKRTREYFVSTAESGTVQMELRYLSYLTGDPKYWITA 221 Query: 178 YNALKALYHHR--SPIGLLGNHIDVMTGRWTAQDAGIGGGIDSYYEYLVKGAILLEKPEL 235 ++ L S GL+ ++++TG + ++ +G DSYYEYL+K + L Sbjct: 222 DKTMEVLLGDATWSHTGLVPITVNLITGAYVGRNIRLGSHGDSYYEYLLKQDLQLFSSGT 281 Query: 236 M--SMFLEARQSIEKYL---KKDDWFVWATMLRG---HVTLPVFQSLESYWPGLL---SL 284 + F + I +YL + F + L G H LP L + PG L + Sbjct: 282 VYRKAFDLSVDGIIEYLLNYTTPNHFAYIAELPGGLEHAQLPKMDHLVCFLPGTLMWGAT 341 Query: 285 IGESDAAMRIIHNY---------------HSVWRQY-----GFTPEVYNLGTGEA----- 319 G S A R N+ + + Y G PE+ + Sbjct: 342 NGTSLEAARTSKNWGTRQERDVKLAQELMRTCYEMYNMTATGLAPEIVFFDVDQTKNEIY 401 Query: 320 SSSRESYPL-RPELIESIMYLYRDTRDPILLQMGEDILRSIQHSARTPCGYA-TIKDVRD 377 S R+ + L RPE +ES+ LYR TRD I + G +I S +R P A T D + Sbjct: 402 SKRRDQHNLMRPETVESLFILYRITRDEIYREWGWNIFVSFLRYSRLPGRDAFTCLDSVE 461 Query: 378 HRK----EDRMESFFLAETTKYLYLLFDPDNFIHNPGVRGTVIDTPNGECVVDLGGYIFN 433 +K D+ ESF+ AET KYLYLLF+ D+F ++ L Y FN Sbjct: 462 SKKVKDQRDKTESFWFAETLKYLYLLFE-DDF-----------------SILPLTNYTFN 503 Query: 434 TEAHPIDPNM 443 TEAHP PN+ Sbjct: 504 TEAHPF-PNI 512 >SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 34.7 bits (76), Expect = 0.043 Identities = 29/113 (25%), Positives = 53/113 (46%), Gaps = 6/113 (5%) Query: 504 IDHTEPKERSDENEPEQNQVLIKTESRTGYVNLDGTERENANESNLSDLVKETINKYYND 563 IDHT+ + P+ + +K+E + V ++ E +++ ++ KE D Sbjct: 315 IDHTQEYMSPIKRTPDFDGNEVKSEELSQTVKVESQETPVHLKADEREIRKEVTETLKGD 374 Query: 564 TINA-ETQENVKHSAEEPTAGSSQTVEVDDIIVPEKSEVQLPSVTKTKEVMNM 615 + Q NVK S EE T E++DI++ +K E+ +TKT+ M + Sbjct: 375 ELRKISLQVNVKFSEEEVTLEDVDDDEIEDILL-DKDEI----LTKTQVWMEL 422 >SPBC23G7.07c |||replication regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 277 Score = 31.5 bits (68), Expect = 0.40 Identities = 26/73 (35%), Positives = 36/73 (49%), Gaps = 5/73 (6%) Query: 549 LSDLVKETINKYYNDTINAETQENV-KHSAEEPTAGSSQTVEVDDIIVPEKSEVQLPSVT 607 LSDL+ I Y+D + E + V K S E T S+ VD+ PE + LP T Sbjct: 88 LSDLLNNYIKSIYSDLTDVELSDKVIKASYIEDTISFSKPKTVDN--YPEYIQ-HLPGFT 144 Query: 608 KTKEVMNMLPKVI 620 K K V N P+++ Sbjct: 145 K-KVVQNSNPEIL 156 >SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase Fab1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1932 Score = 31.1 bits (67), Expect = 0.53 Identities = 21/78 (26%), Positives = 36/78 (46%), Gaps = 7/78 (8%) Query: 514 DENEPEQNQVLIKTESRTGYVNLDGT-----EREN--ANESNLSDLVKETINKYYNDTIN 566 DEN ++N + E+R N G EREN +NE N + ++ + + Sbjct: 1461 DENSEDENGINDTKENRATESNFSGVDSMSKERENVSSNEDNSPEAFEDIFGILFKNESG 1520 Query: 567 AETQENVKHSAEEPTAGS 584 E Q+N++ S++ GS Sbjct: 1521 LEEQQNLEPSSQMDKEGS 1538 >SPAC1751.03 ||SPAC31A2.01|translation initiation factor eIF3m|Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 30.7 bits (66), Expect = 0.70 Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 1/49 (2%) Query: 415 VIDTPNGECVVDLGGYIFNTEAHPIDPNMLYCCHEARQGINISEVYSIY 463 V+D+ E V +L Y+ N EA+ D N+L C E N+ EV +++ Sbjct: 9 VVDSSVEEQVEELAMYLDNLEANT-DKNVLALCREYLASENVKEVLNLF 56 >SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual Length = 656 Score = 29.9 bits (64), Expect = 1.2 Identities = 18/71 (25%), Positives = 36/71 (50%), Gaps = 5/71 (7%) Query: 511 ERSDENEPEQNQVLIKT-----ESRTGYVNLDGTERENANESNLSDLVKETINKYYNDTI 565 E SDE E + N+ K+ ++ GY +G + E+A + + S L+K + Sbjct: 585 ELSDEEEDDTNKSTSKSPKTPKNTKFGYQEFEGIDEEDAQDYSPSPLIKSFNELMFEGEE 644 Query: 566 NAETQENVKHS 576 + E +E+V+++ Sbjct: 645 DEEGEEDVENA 655 >SPCC962.02c |bir1|cut17, pbh1, SPCP31B10.10c|survivin homolog|Schizosaccharomyces pombe|chr 3|||Manual Length = 997 Score = 29.9 bits (64), Expect = 1.2 Identities = 23/74 (31%), Positives = 37/74 (50%), Gaps = 4/74 (5%) Query: 508 EPKERSDENEPEQNQVLIKTESRTGYVNLD-GTERENANESNLSDLVKETINKYYNDTIN 566 +PK E PE+ + I ES+T V++D G + S +SD E +N + + + Sbjct: 647 KPKTEISEVLPEEKRKAICDESQTVRVSIDRGVTKTRDVSSPVSDEKSENVN--HEEANS 704 Query: 567 AETQENVKHSAEEP 580 T NV HS+ +P Sbjct: 705 GHTVMNV-HSSLDP 717 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 29.5 bits (63), Expect = 1.6 Identities = 13/34 (38%), Positives = 22/34 (64%) Query: 560 YYNDTINAETQENVKHSAEEPTAGSSQTVEVDDI 593 Y+ D++N ET+++V S E S++T E+D I Sbjct: 666 YFIDSLNLETKDSVLESNWERFISSTETYELDSI 699 >SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 396 Score = 28.7 bits (61), Expect = 2.8 Identities = 11/28 (39%), Positives = 20/28 (71%) Query: 599 SEVQLPSVTKTKEVMNMLPKVIQDFLNS 626 +EV++ +VT+ E+ N +PK I+D N+ Sbjct: 105 AEVKMMAVTEINEISNKIPKSIEDLENT 132 >SPBC216.01c ||SPBC713.13c|DNA damage response protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 836 Score = 28.7 bits (61), Expect = 2.8 Identities = 10/50 (20%), Positives = 30/50 (60%) Query: 566 NAETQENVKHSAEEPTAGSSQTVEVDDIIVPEKSEVQLPSVTKTKEVMNM 615 + + +E++ ++ +E + G ++ E+D+ I ++++PS+ + +V M Sbjct: 3 STKEEEDLNNNIDEISDGENEEEELDEAIAAALQKIEVPSIPRRVKVYEM 52 >SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schizosaccharomyces pombe|chr 1|||Manual Length = 695 Score = 28.7 bits (61), Expect = 2.8 Identities = 26/106 (24%), Positives = 40/106 (37%), Gaps = 5/106 (4%) Query: 564 TINAETQENVKHSAEEPTAGSSQTVEVD-DIIVPEKSEVQLPSVTKTKEVMNMLPKVIQD 622 T + E + E P+A S+TV D + E S V + K V +++ D Sbjct: 8 TSEYDETEGFTSNQEGPSAAPSKTVASDWHEVKTEDSRVYYYNSVTRKSVWEKPEELMND 67 Query: 623 FLNS----DWKSKPKCEPQKMLERIRREQKYPDHPDVYKYELLLTP 664 F WK + +K + + D PD YK L+ P Sbjct: 68 FEKKLSKLAWKEYATADGKKYWYNVNTRESVWDIPDEYKAALVDEP 113 >SPCC63.08c |ppk36|atg1|serine/threonine protein kinase Ppk36|Schizosaccharomyces pombe|chr 3|||Manual Length = 830 Score = 27.9 bits (59), Expect = 4.9 Identities = 14/34 (41%), Positives = 19/34 (55%) Query: 651 DHPDVYKYELLLTPAPSFLQRISLAGEFLNKKQL 684 DHP VYK L T F Q+++ A +FL + L Sbjct: 120 DHPPVYKAGLNETLVRHFTQQLASALQFLRSRSL 153 >SPBP8B7.28c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 215 Score = 27.9 bits (59), Expect = 4.9 Identities = 19/78 (24%), Positives = 38/78 (48%), Gaps = 7/78 (8%) Query: 522 QVLIKTESRTGYVNLDGTEREN---ANESNLSDLV----KETINKYYNDTINAETQENVK 574 Q+ + ++ G NL+ + + ++S+L D + K+TIN Y D ++ T E Sbjct: 118 QICVHSQRNDGDDNLESDKFVDPFIGDDSDLDDDIYIHDKQTINSEYADDVSDNTDEERT 177 Query: 575 HSAEEPTAGSSQTVEVDD 592 S + + S++ + DD Sbjct: 178 ESKGQQESNSAEEYDDDD 195 >SPCC965.05c |thp1||uracil DNA N-glycosylase Thp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 325 Score = 27.9 bits (59), Expect = 4.9 Identities = 19/49 (38%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Query: 38 IDALDTLAIMGNYSEFNRVVDIVLQKRHFDADINVSVFETNIRIVGGLL 86 I+ DT N SEFN V +KR FD D N+ + E+ GG+L Sbjct: 4 IETRDTGTKNDNSSEFNLSVKSHKRKRSFD-DENLELEESREETSGGIL 51 >SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1023 Score = 27.9 bits (59), Expect = 4.9 Identities = 20/63 (31%), Positives = 28/63 (44%), Gaps = 2/63 (3%) Query: 602 QLPSVTKTKEVMNMLPKVIQDFLNSDWKSKPKCEPQKMLERIRREQKYPDHPDVYKYELL 661 +LPS K + +P V +FL SD + PQ E +K P D+ E+L Sbjct: 177 RLPSQAKKTSAIERVP-VNLNFLQSD-NLVVQSSPQTNFENFEFPKKIPSKEDLETREVL 234 Query: 662 LTP 664 L P Sbjct: 235 LLP 237 >SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 4196 Score = 27.9 bits (59), Expect = 4.9 Identities = 26/108 (24%), Positives = 52/108 (48%), Gaps = 15/108 (13%) Query: 321 SSRESYPLRPELIESIMYLYRDTRDPILLQMGEDILRSIQHSARTPCGYATIKDVRDHRK 380 +S ++Y L++ YLY+ + P LL+ + +++ S ++ Y ++ K Sbjct: 1568 NSSKTYMHLVTLLKERSYLYKVSNIPSLLESAAKLSTTLEDSKKSLLKYFELQ----RHK 1623 Query: 381 EDRMESFFLAETTKYLYLLFDP-DNFIHN-------PGVRGTVIDTPN 420 R+ +FL + + L+ +P D F+ N PG+R ++DT N Sbjct: 1624 ISRL--YFLGDDD-LMELISNPCDPFVINKQIIKLYPGIRSLIVDTEN 1668 >SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyces pombe|chr 2|||Manual Length = 503 Score = 27.5 bits (58), Expect = 6.5 Identities = 15/94 (15%), Positives = 46/94 (48%), Gaps = 4/94 (4%) Query: 510 KERSDENEPEQNQVLIKTESRTGYVNLDGTERENANESNLSDLVKETINKYYNDTINAET 569 + ++D + ++ + + + +N + E+ +NLSD + I + ++A++ Sbjct: 8 ESKTDWASLDSDEEVQRISDKVNQLNTSENKNEDQKATNLSDRLGPKIT----ENVDAKS 63 Query: 570 QENVKHSAEEPTAGSSQTVEVDDIIVPEKSEVQL 603 +++ + A + Q+ + ++P K+EV++ Sbjct: 64 EQDKATNTIAEDANTKQSENDESNLIPNKNEVRV 97 >SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1147 Score = 27.1 bits (57), Expect = 8.6 Identities = 17/82 (20%), Positives = 43/82 (52%), Gaps = 1/82 (1%) Query: 546 ESNLSDLVKETINKYYNDTINAETQENVKHSAEEPTAGSSQTVEVDDIIVPEKSEVQLPS 605 ++N + K T + +T++A+ +N+K + ++ GSS + ++ ++ + Sbjct: 837 DANANIYDKLTADHTNYETVSADINQNLKETLDKLLNGSSDFKNNEIELLHDQIRITNAK 896 Query: 606 VTKTKEVMNMLPKVIQDFLNSD 627 + K ++++N K I+D L S+ Sbjct: 897 LEKREKLIN-ASKYIEDTLRSE 917 >SPBC21H7.02 |taf10||transcription factor TFIID complex subunit Taf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 215 Score = 27.1 bits (57), Expect = 8.6 Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 3/44 (6%) Query: 562 NDTINAETQENVKHSAEEPTAGSSQTVEVDDIIVPEKSEVQLPS 605 +D N E EN++ +EP G++ ++ VD+ PE S LP+ Sbjct: 2 SDINNNEPAENIEQPKQEPEDGNN-SMSVDE--QPETSSTNLPT 42 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.317 0.136 0.404 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,216,333 Number of Sequences: 5004 Number of extensions: 141063 Number of successful extensions: 416 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 394 Number of HSP's gapped (non-prelim): 23 length of query: 686 length of database: 2,362,478 effective HSP length: 78 effective length of query: 608 effective length of database: 1,972,166 effective search space: 1199076928 effective search space used: 1199076928 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 57 (27.1 bits)
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