BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001008-TA|BGIBMGA001008-PA|IPR007114|Major facilitator superfamily, IPR011701|Major facilitator superfamily MFS_1 (540 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17A2.01 |bsu1|SPAC1B1.05, bsu1|high-affinity import carrier ... 35 0.025 SPCC61.01c |str2|str1, SPCC622.20c|siderophore-iron transporter ... 32 0.23 SPCC63.10c |||dolichol kinase |Schizosaccharomyces pombe|chr 3||... 30 0.71 SPBC19F8.06c |meu22||amino acid permease, unknown 11|Schizosacch... 30 0.71 SPCC1235.13 |ght6|meu12|hexose transporter Ght6 |Schizosaccharom... 30 0.94 SPBC609.04 |caf5||spermine family transmembrane transporter Caf5... 29 1.2 SPCC1235.14 |ght5||hexose transporter Ght5 |Schizosaccharomyces ... 29 1.6 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 29 1.6 SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|c... 29 2.2 SPBC1734.12c |alg12||dolichyl pyrophosphate Man7GlcNAc2 alpha-1,... 28 3.8 SPAPB17E12.12c |||mitochondrial transporter|Schizosaccharomyces ... 27 5.0 SPAC1952.10c |||conserved fungal protein |Schizosaccharomyces po... 27 5.0 SPAC25B8.17 |||peptidase family A22|Schizosaccharomyces pombe|ch... 27 5.0 SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces pomb... 27 6.6 SPBC8D2.02c |||vacuolar sorting protein Vps68|Schizosaccharomyce... 27 6.6 >SPAC17A2.01 |bsu1|SPAC1B1.05, bsu1|high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1|Schizosaccharomyces pombe|chr 1|||Manual Length = 526 Score = 35.1 bits (77), Expect = 0.025 Identities = 41/166 (24%), Positives = 66/166 (39%), Gaps = 9/166 (5%) Query: 17 WVITFAYALNNVVVLPLISSFGLVFQEAFDETGLTPTQGTLVIILNHSIGMLLSFFGGPL 76 W I F + L + V+ + FG + + ++ TL + +N L F GPL Sbjct: 81 WSIVFMFCLMQIYVIWTSNGFGSIEYSVMAQFNVSAQVATLCLSMNILGSGLGPMFLGPL 140 Query: 77 LRRFGYRKVAAVGAILISAGLILTSLASSFWVFILSYSILNSMGVASVMAAFTLAINSFF 136 G + V + + I +L + I+S+ I+ G ++ + I F Sbjct: 141 SDIGGRKPVYFCSIFVYTVFNISCALPRNIVQMIISHFIIGVAG-STALTNVAGGIPDLF 199 Query: 137 KEKRSRAIGVGMSV----TGLGAIYMPLLMSTLIYA-YGWRYAVLI 177 E + GV MS+ GAI P+ I A YGWR+ I Sbjct: 200 PEDTA---GVPMSLFVWACAGGAIGAPMATGVDINAKYGWRWLYYI 242 >SPCC61.01c |str2|str1, SPCC622.20c|siderophore-iron transporter Str2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 597 Score = 31.9 bits (69), Expect = 0.23 Identities = 28/113 (24%), Positives = 50/113 (44%), Gaps = 5/113 (4%) Query: 83 RKVAAVGAILISAGLILTSLASSFWVFILSYSILNSMGVASVMAAFTLAINSFFKEKRSR 142 RK A G LI S +F L + L+ +G+ ++ FTL + F S+ Sbjct: 248 RKAKAAGLYREHHNLINHSTPEKLRLFYLFWQELDGLGIVLFVSGFTLLLLPF--SHSSQ 305 Query: 143 AIGVGMSVTGLGAIYM--PLLMSTLIYAYGW-RYAVLILSAICLHSLLAACLL 192 + ++ L I + LL++ Y + RY V L ++ ++L +C+L Sbjct: 306 VVSPDSTILTLFTITLSIALLVTLCFYDVKYARYPVFALKSLKDRTILGSCVL 358 >SPCC63.10c |||dolichol kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 465 Score = 30.3 bits (65), Expect = 0.71 Identities = 21/108 (19%), Positives = 53/108 (49%), Gaps = 10/108 (9%) Query: 367 LPIILKDMLKFETSEIAQIMSII--GFSDTLFRLVAPFIGEW----WNKSPRVMYMVSLT 420 +PI L + LK + + ++ ++ G D++ ++ G+ NKS ++ S++ Sbjct: 350 IPIWLSNALKGPVASVELLVGVLCLGCGDSMASIIGKRFGKHRISKTNKSIEGVFAFSIS 409 Query: 421 LVIIIRTIMLF----THSYLGMVFVAVAMGFTKGIRTVYMNLIIPCYV 464 + +++ F + ++ +F+++ +G+ T NLI+P Y+ Sbjct: 410 VFLVLHLTQAFHVCPSVTFWKTLFMSLCTAILEGVSTENDNLILPMYM 457 >SPBC19F8.06c |meu22||amino acid permease, unknown 11|Schizosaccharomyces pombe|chr 2|||Manual Length = 574 Score = 30.3 bits (65), Expect = 0.71 Identities = 21/62 (33%), Positives = 29/62 (46%) Query: 399 VAPFIGEWWNKSPRVMYMVSLTLVIIIRTIMLFTHSYLGMVFVAVAMGFTKGIRTVYMNL 458 VAP I +WN S V +S+ VIII + T Y + F M I V +++ Sbjct: 161 VAPLIINFWNASGPVSIWISVFYVIIIAINIWGTEGYGEVEFFLSIMKVISVIGFVILSI 220 Query: 459 II 460 II Sbjct: 221 II 222 >SPCC1235.13 |ght6|meu12|hexose transporter Ght6 |Schizosaccharomyces pombe|chr 3|||Manual Length = 535 Score = 29.9 bits (64), Expect = 0.94 Identities = 22/73 (30%), Positives = 36/73 (49%), Gaps = 5/73 (6%) Query: 54 QGTLVIILNH--SIGMLLSFFGGPLLRRFGYRKVAAVGAILISAGLILTSLASSFWVFIL 111 QG LV ++N ++G LLS PL RFG RK ++ G+I+ WV ++ Sbjct: 58 QGLLVGMVNTGTTVGCLLS---SPLGDRFGKRKCIMGWTLVYITGVIVQLTTIPSWVQMM 114 Query: 112 SYSILNSMGVASV 124 I +G+ ++ Sbjct: 115 VAKIWTGLGIGAL 127 >SPBC609.04 |caf5||spermine family transmembrane transporter Caf5|Schizosaccharomyces pombe|chr 2|||Manual Length = 531 Score = 29.5 bits (63), Expect = 1.2 Identities = 37/186 (19%), Positives = 76/186 (40%), Gaps = 13/186 (6%) Query: 19 ITFAYALNNVVVLPLISSFGLVFQEAFDETGLTPTQGTLVIILNHSIGMLLSFFGGPL-- 76 +TFA + + + P IS QE + G + +L ++IG L+ PL Sbjct: 92 LTFAVYVGSAIFTPGISEM----QETMHVGTVPVVLGLTLFVLGYAIGPLIL---SPLSE 144 Query: 77 LRRFGYRKVAAVGAILISAGLILTSLASSFWVFILSYSILNSMGVASVMAAFTLAINSFF 136 + + G +K+ + ++ I T+L SS V + + G + +A + + Sbjct: 145 IPQVGRQKIYVLSLLVFVCLQIPTALGSSLGVLLPMRFLAGFFG-SPALATGGATLADIW 203 Query: 137 KEKRSRAIGVGMSVTGLGA-IYMPLLMSTLIYAYGWRYAVLILSAICLHSLLAACLLRP- 194 + ++ +G + PLL ++ A WR+ +L I +L+ P Sbjct: 204 QPWLLPYFMCFWAIGAIGGPVLGPLLGGAMVVAESWRWQFWLLMMISGFALIVIFFFMPE 263 Query: 195 -AKWYL 199 ++W++ Sbjct: 264 TSEWHI 269 >SPCC1235.14 |ght5||hexose transporter Ght5 |Schizosaccharomyces pombe|chr 3|||Manual Length = 546 Score = 29.1 bits (62), Expect = 1.6 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 2/58 (3%) Query: 71 FFGGPLLRRFGYRKVAAVGAILISAGLILTSLASSFWVFILSYSILN--SMGVASVMA 126 F PL+ R G R I+ G+IL A WV I+ I S+G SV+A Sbjct: 74 FLSSPLMDRIGKRTSIMFWTIVYLIGIILQVTAVPSWVQIMVAKIWTGLSIGALSVLA 131 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 29.1 bits (62), Expect = 1.6 Identities = 27/100 (27%), Positives = 47/100 (47%), Gaps = 4/100 (4%) Query: 75 PLLRRFGYRKVAAVGAIL-ISAGLILTSLASSFWVFILSYSILNSMGVASVMAAFTLAIN 133 PL RF + + VG+ L + AGL++T+ S+ W + IL +V T + Sbjct: 474 PLHDRFHKKMLFFVGSCLFVLAGLLITTFVSNVWGRYVGLLILGFGLGPTVPIIMTWVSS 533 Query: 134 SFFKEKRSRAIGVGMS-VTGLGAIYMPLLMSTLIYAYGWR 172 + + G++ V+GLG + + S L Y+ GW+ Sbjct: 534 AMGPSHGDVGVAAGLAIVSGLGNLGSVVATSAL-YS-GWK 571 >SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 581 Score = 28.7 bits (61), Expect = 2.2 Identities = 15/56 (26%), Positives = 27/56 (48%), Gaps = 1/56 (1%) Query: 118 SMGVASVMAAFTLAINSFFKEKRSRAIGVGMSVTGLGAIYMPLLMSTLIYAYGWRY 173 S A + F+++ FF+ +R IG+ + T +G PL + + GWR+ Sbjct: 160 SEACAEALTQFSVS-EVFFEHERGIKIGIYILSTSVGTYLGPLAAGYIASSQGWRW 214 >SPBC1734.12c |alg12||dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase Alg12 |Schizosaccharomyces pombe|chr 2|||Manual Length = 546 Score = 27.9 bits (59), Expect = 3.8 Identities = 18/64 (28%), Positives = 36/64 (56%), Gaps = 1/64 (1%) Query: 340 LLKDPIFVSILIGLSVAACVETNFSLLLPIILKDMLKFETSEIAQIMSIIGFSDTLFRLV 399 LLK+ + SI I + AA V + +LLL ++ +L +++++ ++G S +L + Sbjct: 155 LLKNNYYGSISILVFAAAIVRSEIALLLMCLILPLLLQRRITLSKLL-LVGISSSLAAVG 213 Query: 400 APFI 403 A F+ Sbjct: 214 ASFL 217 >SPAPB17E12.12c |||mitochondrial transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 317 Score = 27.5 bits (58), Expect = 5.0 Identities = 27/86 (31%), Positives = 35/86 (40%), Gaps = 3/86 (3%) Query: 7 YKVPPDGGYGWVITFAYA-LNNVVVLPLISSFGLVF--QEAFDETGLTPTQGTLVIILNH 63 Y V +GGY + A L NVV I F F Q+ F LT Q + Sbjct: 178 YIVQHEGGYPALYNGLPATLLNVVPYVSICFFTFEFCKQKFFSNADLTAFQKLFLGGFTG 237 Query: 64 SIGMLLSFFGGPLLRRFGYRKVAAVG 89 IG L+F L RRF ++ +G Sbjct: 238 IIGQTLTFPADVLRRRFQVNRIPGIG 263 >SPAC1952.10c |||conserved fungal protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 361 Score = 27.5 bits (58), Expect = 5.0 Identities = 22/103 (21%), Positives = 50/103 (48%), Gaps = 6/103 (5%) Query: 332 FVDFFDLTLLKDPIFVSILIGLSVAACVETNFSLLLPIILKDMLK-FETSEIAQIMSIIG 390 F F +T+ K +F++ ++ + A F + P +L + ++ Q+ +++ Sbjct: 43 FHHFRLITVEKIDVFINAMVPSTFAL-----FCMACPYLLYTAVDALLNKDVIQMSALLI 97 Query: 391 FSDTLFRLVAPFIGEWWNKSPRVMYMVSLTLVIIIRTIMLFTH 433 F +LF L + ++K PR +++ +L L+ IR + F + Sbjct: 98 FHVSLFCLSVVQYFQIFSKDPRAIFLSALYLIDPIRLVTSFNY 140 >SPAC25B8.17 |||peptidase family A22|Schizosaccharomyces pombe|chr 1|||Manual Length = 295 Score = 27.5 bits (58), Expect = 5.0 Identities = 15/38 (39%), Positives = 20/38 (52%) Query: 148 MSVTGLGAIYMPLLMSTLIYAYGWRYAVLILSAICLHS 185 +S+ GLG I MP LM L+Y + Y + S HS Sbjct: 183 LSMLGLGDIVMPGLMLALMYRFDLHYYINSTSQPKKHS 220 >SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces pombe|chr 2|||Manual Length = 574 Score = 27.1 bits (57), Expect = 6.6 Identities = 16/51 (31%), Positives = 27/51 (52%), Gaps = 2/51 (3%) Query: 14 GYGWVITFAYALNNVVVLPLISSFGLVFQEAFDETGLTPTQGTLVIILNHS 64 GY FA++ + +VV+ S+FG E ET + LVI++N++ Sbjct: 427 GYAIASAFAHSSDKIVVVEGDSAFGFSAMEL--ETAIRNQLDLLVIVINNN 475 >SPBC8D2.02c |||vacuolar sorting protein Vps68|Schizosaccharomyces pombe|chr 2|||Manual Length = 170 Score = 27.1 bits (57), Expect = 6.6 Identities = 14/46 (30%), Positives = 21/46 (45%) Query: 91 ILISAGLILTSLASSFWVFILSYSILNSMGVASVMAAFTLAINSFF 136 + I L+ L SF VFIL Y + G + +M + + N F Sbjct: 102 LFIGFALLAGGLGGSFTVFILKYVVAGYEGKSLLMGSANIISNILF 147 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.324 0.139 0.417 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,103,208 Number of Sequences: 5004 Number of extensions: 78811 Number of successful extensions: 244 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 233 Number of HSP's gapped (non-prelim): 20 length of query: 540 length of database: 2,362,478 effective HSP length: 76 effective length of query: 464 effective length of database: 1,982,174 effective search space: 919728736 effective search space used: 919728736 T: 11 A: 40 X1: 15 ( 7.0 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.6 bits) S2: 56 (26.6 bits)
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