Silkworm BLAST Result against tribolium

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001001-TA|BGIBMGA001001-PA|IPR001092|Basic
helix-loop-helix dimerisation region bHLH, IPR011598|Helix-loop-helix
DNA-binding
         (193 letters)

Database: tribolium 
           317 sequences; 114,650 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY321476-1|AAQ23386.1|  253|Tribolium castaneum Ash protein.          100   9e-24
AY321476-2|AAQ23387.1|  257|Tribolium castaneum Ase protein.           75   3e-16
AJ536347-1|CAD60438.1|   40|Tribolium castaneum putative Ash pro...    39   2e-05
AJ829922-1|CAH25640.1|  193|Tribolium castaneum twist bHLH trans...    31   0.005
U61151-1|AAB41306.1|   45|Tribolium castaneum TATH1 protein.           26   0.23 
U09586-2|AAC47271.1|  712|Tribolium castaneum protease, reverse ...    21   6.5  

>AY321476-1|AAQ23386.1|  253|Tribolium castaneum Ash protein.
          Length = 253

 Score =  100 bits (239), Expect = 9e-24
 Identities = 77/201 (38%), Positives = 101/201 (50%), Gaps = 33/201 (16%)

Query: 24  PAPRRLAPAPVDTT--RCKKRSYLHQPY--PTQPASXXXXXXXXXXXXKQVNNGFAALRQ 79
           PAP R +    + +  RCK++     PY    QPAS            KQVNNGFA LRQ
Sbjct: 52  PAPERTSVLVTNNSDLRCKRKIQF-MPYGPQQQPASVARRNARERNRVKQVNNGFATLRQ 110

Query: 80  HIPSAVTAALAG-----GRGSSRKLSKVDTLRLAVEYIKSLKRLL-DESDDGCSDTQLG- 132
           HIP++V AA A      GRG+S+KLSKV+TLRLAVEYI+SLK+++ D  +D   ++  G 
Sbjct: 111 HIPASVAAAFAPQGPSTGRGASKKLSKVETLRLAVEYIRSLKQMIEDHENDTTGNSGEGS 170

Query: 133 -------------------LGLSTTGSQTXXXXXXXXXXXXXFVSESSAGPNCH--DVYD 171
                              L  + T S+              F + SS     +  + ++
Sbjct: 171 LQENRYYNNSPDQYQSYQILLPTPTCSEASSSPTPSHSSESSFSAASSYTNTIYHQENFE 230

Query: 172 AYEPMSPEDEELLDVISWWQQ 192
            YEP SPEDEELLD I  WQQ
Sbjct: 231 NYEPKSPEDEELLDAIFSWQQ 251


>AY321476-2|AAQ23387.1|  257|Tribolium castaneum Ase protein.
          Length = 257

 Score = 75.4 bits (177), Expect = 3e-16
 Identities = 45/100 (45%), Positives = 58/100 (58%), Gaps = 3/100 (3%)

Query: 35  DTTRCKKRSYLHQPYPTQPASXXXXXXXXXXXXKQVNNGFAALRQHIPSAVTAAL-AGGR 93
           D    KK+S        QP +            KQVNNGFA LRQHIP+ + AA  +  R
Sbjct: 33  DILLTKKKSKPGGKTAPQPVAVARRNARERNRVKQVNNGFANLRQHIPNFIAAAFESNSR 92

Query: 94  GSSRKLSKVDTLRLAVEYIKSLKRL--LDESDDGCSDTQL 131
           G ++KLSKV+TLR+AVEYI+SL+ L  LDES    S + +
Sbjct: 93  GGNKKLSKVETLRMAVEYIRSLEDLLALDESTVSSSTSSI 132



 Score = 23.4 bits (48), Expect = 1.2
 Identities = 6/11 (54%), Positives = 10/11 (90%)

Query: 183 LLDVISWWQQQ 193
           ++DV+ WW+QQ
Sbjct: 239 VMDVMQWWEQQ 249


>AJ536347-1|CAD60438.1|   40|Tribolium castaneum putative Ash
          protein protein.
          Length = 40

 Score = 39.1 bits (87), Expect = 2e-05
 Identities = 18/32 (56%), Positives = 24/32 (75%), Gaps = 1/32 (3%)

Query: 69 QVNNGFAALRQHIPSAVTAAL-AGGRGSSRKL 99
          +VNNGFA LRQHIP+ + AA  +  RG ++KL
Sbjct: 7  KVNNGFANLRQHIPNFIAAAFESNSRGGNKKL 38


>AJ829922-1|CAH25640.1|  193|Tribolium castaneum twist bHLH
           transcription factor protein.
          Length = 193

 Score = 31.5 bits (68), Expect = 0.005
 Identities = 23/64 (35%), Positives = 33/64 (51%), Gaps = 12/64 (18%)

Query: 68  KQVNNGFAALRQHIPSAVTAALAGGRGSSRKLSKVDTLRLAVEYIKSLKRLLDESDDGCS 127
           + +N  FA+LR+ IP+            S KLSK+ TL+LA  YI  L  +L  S++   
Sbjct: 110 QSLNEAFASLRKSIPTM----------PSDKLSKIQTLKLAARYIDFLYHVL--SNENAL 157

Query: 128 DTQL 131
           D  L
Sbjct: 158 DVDL 161


>U61151-1|AAB41306.1|   45|Tribolium castaneum TATH1 protein.
          Length = 45

 Score = 25.8 bits (54), Expect = 0.23
 Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 9/42 (21%)

Query: 70  VNNGFAALRQHIPSAVTAALAGGRGSSRKLSKVDTLRLAVEY 111
           +N+ F  LR  +PS          G+ RKLSK +TL++A  Y
Sbjct: 13  LNDAFDRLRDVVPSL---------GNDRKLSKFETLQMAQTY 45


>U09586-2|AAC47271.1|  712|Tribolium castaneum protease, reverse
           transcriptase andRNase H protein.
          Length = 712

 Score = 21.0 bits (42), Expect = 6.5
 Identities = 8/30 (26%), Positives = 15/30 (50%)

Query: 17  KLTHLQTPAPRRLAPAPVDTTRCKKRSYLH 46
           K  HL++P+  +    P+     +KR  +H
Sbjct: 246 KKQHLESPSALQAKVDPLKIPEAEKRKLIH 275


  Database: tribolium
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 114,650
  Number of sequences in database:  317
  
Lambda     K      H
   0.314    0.129    0.384 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 39,478
Number of Sequences: 317
Number of extensions: 1286
Number of successful extensions: 10
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8
length of query: 193
length of database: 114,650
effective HSP length: 53
effective length of query: 140
effective length of database: 97,849
effective search space: 13698860
effective search space used: 13698860
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.5 bits)
S2: 41 (20.6 bits)