Silkworm BLAST Result against tribolium

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001000-TA|BGIBMGA001000-PA|IPR001092|Basic
helix-loop-helix dimerisation region bHLH, IPR011598|Helix-loop-helix
DNA-binding
         (187 letters)

Database: tribolium 
           317 sequences; 114,650 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY321476-1|AAQ23386.1|  253|Tribolium castaneum Ash protein.           72   3e-15
AY321476-2|AAQ23387.1|  257|Tribolium castaneum Ase protein.           69   3e-14
AJ829922-1|CAH25640.1|  193|Tribolium castaneum twist bHLH trans...    33   0.001
U61151-1|AAB41306.1|   45|Tribolium castaneum TATH1 protein.           29   0.018
S73230-1|AAB30812.1|   35|Tribolium castaneum twist protein.           29   0.018
DQ414248-1|ABD63010.2|  311|Tribolium castaneum sloppy-paired pr...    21   8.2  

>AY321476-1|AAQ23386.1|  253|Tribolium castaneum Ash protein.
          Length = 253

 Score = 71.7 bits (168), Expect = 3e-15
 Identities = 57/172 (33%), Positives = 77/172 (44%), Gaps = 20/172 (11%)

Query: 32  QAASIARRNARERNRVKQVNDGFNALRKRLPXXXXXXXX---XXXXXXXXXXXSKVDTLR 88
           Q AS+ARRNARERNRVKQVN+GF  LR+ +P                      SKV+TLR
Sbjct: 83  QPASVARRNARERNRVKQVNNGFATLRQHIPASVAAAFAPQGPSTGRGASKKLSKVETLR 142

Query: 89  MVVEYIRYLQNMI-DESDAALGIPKQPSI------DLSTISYEADDGVF-------ERSS 134
           + VEYIR L+ MI D  +   G   + S+      + S   Y++   +          SS
Sbjct: 143 LAVEYIRSLKQMIEDHENDTTGNSGEGSLQENRYYNNSPDQYQSYQILLPTPTCSEASSS 202

Query: 135 PYTDXXXXXXXXXXXXXXXXNYSQGYIPNFQIEEQITPMDDDLLNTISWWQE 186
           P                    Y Q    NF+  E  +P D++LL+ I  WQ+
Sbjct: 203 PTPSHSSESSFSAASSYTNTIYHQ---ENFENYEPKSPEDEELLDAIFSWQQ 251


>AY321476-2|AAQ23387.1|  257|Tribolium castaneum Ase protein.
          Length = 257

 Score = 68.5 bits (160), Expect = 3e-14
 Identities = 39/86 (45%), Positives = 50/86 (58%), Gaps = 3/86 (3%)

Query: 32  QAASIARRNARERNRVKQVNDGFNALRKRLPXXXXXXXXXXXXXXXXXXXSKVDTLRMVV 91
           Q  ++ARRNARERNRVKQVN+GF  LR+ +P                   SKV+TLRM V
Sbjct: 50  QPVAVARRNARERNRVKQVNNGFANLRQHIP-NFIAAAFESNSRGGNKKLSKVETLRMAV 108

Query: 92  EYIRYLQNM--IDESDAALGIPKQPS 115
           EYIR L+++  +DES  +      PS
Sbjct: 109 EYIRSLEDLLALDESTVSSSTSSIPS 134


>AJ829922-1|CAH25640.1|  193|Tribolium castaneum twist bHLH
           transcription factor protein.
          Length = 193

 Score = 33.5 bits (73), Expect = 0.001
 Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 12/67 (17%)

Query: 40  NARERNRVKQVNDGFNALRKRLPXXXXXXXXXXXXXXXXXXXSKVDTLRMVVEYIRYLQN 99
           N RER R + +N+ F +LRK +P                   SK+ TL++   YI +L +
Sbjct: 102 NVRERQRTQSLNEAFASLRKSIP------------TMPSDKLSKIQTLKLAARYIDFLYH 149

Query: 100 MIDESDA 106
           ++   +A
Sbjct: 150 VLSNENA 156


>U61151-1|AAB41306.1|   45|Tribolium castaneum TATH1 protein.
          Length = 45

 Score = 29.5 bits (63), Expect = 0.018
 Identities = 12/23 (52%), Positives = 16/23 (69%)

Query: 40 NARERNRVKQVNDGFNALRKRLP 62
          NARER R+  +ND F+ LR  +P
Sbjct: 3  NARERRRMNSLNDAFDRLRDVVP 25


>S73230-1|AAB30812.1|   35|Tribolium castaneum twist protein.
          Length = 35

 Score = 29.5 bits (63), Expect = 0.018
 Identities = 11/23 (47%), Positives = 16/23 (69%)

Query: 40 NARERNRVKQVNDGFNALRKRLP 62
          N RER R + +N+ F +LRK +P
Sbjct: 2  NVRERQRTQSLNEAFASLRKSIP 24


>DQ414248-1|ABD63010.2|  311|Tribolium castaneum sloppy-paired
           protein.
          Length = 311

 Score = 20.6 bits (41), Expect = 8.2
 Identities = 9/14 (64%), Positives = 11/14 (78%)

Query: 1   MVAIAPAPETRLTL 14
           M+AI  +PE RLTL
Sbjct: 93  MMAIRNSPEKRLTL 106


  Database: tribolium
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 114,650
  Number of sequences in database:  317
  
Lambda     K      H
   0.314    0.131    0.378 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 34,667
Number of Sequences: 317
Number of extensions: 1056
Number of successful extensions: 10
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8
length of query: 187
length of database: 114,650
effective HSP length: 53
effective length of query: 134
effective length of database: 97,849
effective search space: 13111766
effective search space used: 13111766
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.5 bits)
S2: 41 (20.6 bits)