BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000997-TA|BGIBMGA000997-PA|IPR002048|Calcium-binding
EF-hand
(285 letters)
Database: bee
429 sequences; 140,377 total letters
Searching.....................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY375535-1|AAQ82648.1| 147|Apis mellifera doublesex protein. 27 0.14
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 26 0.33
DQ435325-1|ABD92640.1| 160|Apis mellifera OBP7 protein. 25 1.0
AB231585-1|BAE17127.1| 898|Apis mellifera Mahya protein. 23 2.3
AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein. 23 4.1
DQ325089-1|ABD14103.1| 185|Apis mellifera complementary sex det... 22 7.1
DQ325088-1|ABD14102.1| 185|Apis mellifera complementary sex det... 22 7.1
DQ325076-1|ABD14090.1| 191|Apis mellifera complementary sex det... 22 7.1
AJ517411-1|CAD56944.1| 1770|Apis mellifera vitellogenin precurso... 21 9.4
AF144379-1|AAD34586.1| 543|Apis mellifera glutamate transporter... 21 9.4
>AY375535-1|AAQ82648.1| 147|Apis mellifera doublesex protein.
Length = 147
Score = 27.5 bits (58), Expect = 0.14
Identities = 13/36 (36%), Positives = 19/36 (52%)
Query: 2 EEEPALPSSVIPTLSNTSQKDNNIEGSLEEKNTDHP 37
EE LP V T+S+ Q ++EGS + + D P
Sbjct: 40 EEVDPLPFGVENTISSVPQPPRSLEGSYDSSSGDSP 75
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 26.2 bits (55), Expect = 0.33
Identities = 16/71 (22%), Positives = 39/71 (54%), Gaps = 3/71 (4%)
Query: 186 IRQRDFEIEDELRSSLQVFDPGRTGTIDREYFREVLAKQGHKMPQKQLDNLIKEV-DMSN 244
I Q D E+ + ++ + F+ T I E ++ ++ + + + K +DN+ KE+ D+
Sbjct: 519 ITQSDIELANTFKAIIYGFNVNATKQIKDEANKKGVSLRFYNVVYKLIDNIKKEIYDILP 578
Query: 245 DGTIGIEDVVG 255
+ + +E+++G
Sbjct: 579 E--VDVEEILG 587
>DQ435325-1|ABD92640.1| 160|Apis mellifera OBP7 protein.
Length = 160
Score = 24.6 bits (51), Expect = 1.0
Identities = 22/89 (24%), Positives = 42/89 (47%), Gaps = 11/89 (12%)
Query: 175 ITFEQFLKMVTIRQRDFEIEDELRSSLQVFDPGRTGTIDREYFREVLAKQGHKMPQKQLD 234
+TF+ F+KM + Q + E ++ L + T +++ + + + QLD
Sbjct: 46 LTFKDFIKMQELLQEEDISEGNIKKYLTNYSCFITCALEKSHIIQ--------NDEIQLD 97
Query: 235 NLIKEVDMSNDGTIGIEDVVGTMCIDLNK 263
L V+M+N I I+ + + CI+ NK
Sbjct: 98 KL---VEMANRKNISIDVKMLSECINANK 123
>AB231585-1|BAE17127.1| 898|Apis mellifera Mahya protein.
Length = 898
Score = 23.4 bits (48), Expect = 2.3
Identities = 11/28 (39%), Positives = 15/28 (53%)
Query: 19 SQKDNNIEGSLEEKNTDHPDENRDNEEV 46
S D N G+LE + + EN D EE+
Sbjct: 243 SHYDRNNNGNLEREELEQFAENEDLEEL 270
>AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein.
Length = 1598
Score = 22.6 bits (46), Expect = 4.1
Identities = 13/45 (28%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Query: 9 SSVIPTLSNTSQKDNNIEGSLEEKNTDHPDENRDNEEVLEATEAE 53
++ IPT ++ N+ EG+ + D +EN + E+ +EAE
Sbjct: 258 TTTIPTRRLRKRRQNDGEGA---DDRDDDEENEEEEDGRGQSEAE 299
>DQ325089-1|ABD14103.1| 185|Apis mellifera complementary sex
determiner protein.
Length = 185
Score = 21.8 bits (44), Expect = 7.1
Identities = 10/40 (25%), Positives = 21/40 (52%)
Query: 2 EEEPALPSSVIPTLSNTSQKDNNIEGSLEEKNTDHPDENR 41
E E + +I +LSN + +NN + + N ++ + N+
Sbjct: 71 ERERSREPKIISSLSNNTIHNNNYKYNYNNNNYNNNNYNK 110
>DQ325088-1|ABD14102.1| 185|Apis mellifera complementary sex
determiner protein.
Length = 185
Score = 21.8 bits (44), Expect = 7.1
Identities = 10/40 (25%), Positives = 21/40 (52%)
Query: 2 EEEPALPSSVIPTLSNTSQKDNNIEGSLEEKNTDHPDENR 41
E E + +I +LSN + +NN + + N ++ + N+
Sbjct: 71 ERERSREPKIISSLSNNTIHNNNYKYNYNNNNYNNNNYNK 110
>DQ325076-1|ABD14090.1| 191|Apis mellifera complementary sex
determiner protein.
Length = 191
Score = 21.8 bits (44), Expect = 7.1
Identities = 12/46 (26%), Positives = 23/46 (50%)
Query: 2 EEEPALPSSVIPTLSNTSQKDNNIEGSLEEKNTDHPDENRDNEEVL 47
E E + +I +LSN + +NN + N ++ + N +N + L
Sbjct: 72 ERERSREPKIISSLSNKTIHNNNNYNNNNYNNYNYNNNNYNNYKKL 117
>AJ517411-1|CAD56944.1| 1770|Apis mellifera vitellogenin precursor
protein.
Length = 1770
Score = 21.4 bits (43), Expect = 9.4
Identities = 11/54 (20%), Positives = 26/54 (48%)
Query: 227 KMPQKQLDNLIKEVDMSNDGTIGIEDVVGTMCIDLNKEDLMMLRASVYPPDEPP 280
+M + +LI + D+ ++ +VG + +D E+ + + + P D+ P
Sbjct: 110 RMKHGLIRDLIVDRDVPTWEVNILKSIVGQLQVDTQGENAVKVNSVQVPTDDEP 163
>AF144379-1|AAD34586.1| 543|Apis mellifera glutamate transporter
Am-EAAT protein.
Length = 543
Score = 21.4 bits (43), Expect = 9.4
Identities = 9/32 (28%), Positives = 19/32 (59%)
Query: 132 IDGYITEKELVYMLKSLLLMTYTPEQLEELIA 163
+D ++ E++ L +++M Y+P + LIA
Sbjct: 265 VDFFMILNEIIMKLVGIIIMWYSPFGIMCLIA 296
Database: bee
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 140,377
Number of sequences in database: 429
Lambda K H
0.311 0.133 0.372
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 74,795
Number of Sequences: 429
Number of extensions: 2933
Number of successful extensions: 11
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3
Number of HSP's gapped (non-prelim): 10
length of query: 285
length of database: 140,377
effective HSP length: 57
effective length of query: 228
effective length of database: 115,924
effective search space: 26430672
effective search space used: 26430672
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)
S2: 43 (21.4 bits)
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