Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000994-TA|BGIBMGA000994-PA|IPR010097|Malate
dehydrogenase, NAD-dependent, eukaryotes and gamma proteobacteria,
IPR001236|Lactate/malate dehydrogenase
(329 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|ch... 163 2e-41
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 46 6e-06
SPAC144.07c |||conserved eukaryotic protein|Schizosaccharomyces ... 28 1.6
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 27 4.9
SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase I|Schizosacchar... 27 4.9
SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit S... 26 8.5
>SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 341
Score = 163 bits (397), Expect = 2e-41
Identities = 96/320 (30%), Positives = 172/320 (53%), Gaps = 9/320 (2%)
Query: 13 TTTRSYQVTVVGATSEVGQVXXXXXXXXXXITKLVVHDNKEFTPGVTLDLSQIPSPSRIL 72
T++R+++V V+GA +GQ +++L + D + PGV D+ I + S ++
Sbjct: 24 TSSRAFKVAVLGAGGGIGQPLSMLLKLNDKVSELALFDIRG-APGVAADIGHINTTSNVV 82
Query: 73 GLTGEDT-LERALKNSDLIIAAGGLSQRPDISEKALLTANSQFIKSIISKLGRSCPIPFV 131
G +D LE+AL +D++I G+ ++P ++ L N+ ++ + G +CP
Sbjct: 83 GYAPDDKGLEKALNGADVVIIPAGVPRKPGMTRDDLFATNASIVRDLAFAAGETCPEAKY 142
Query: 132 GIVTEPINYLIPMTAEIMRNHGVYDEKKLFGITEIDAIRSQCLYATENNLKANDCYVPVI 191
+VT P+N +P+ + + GV+ K LFG+T +D++R+ + N KA ++PV+
Sbjct: 143 LVVTNPVNSTVPIFKKALERVGVHQPKHLFGVTTLDSVRASRFTSQVTNGKAELLHIPVV 202
Query: 192 GGHSDKTIVPLLSQAKPTVEMKEKDLEEFTIKLRNCDGMITKAKRGC-IPNLSVAYSSFL 250
GGHS TIVPLLSQ VE+ + + +++ + KAK G LS+AY+
Sbjct: 203 GGHSGATIVPLLSQG--GVELTGEKRDALIHRIQFGGDEVVKAKAGAGSATLSMAYAGAR 260
Query: 251 FTKSILDALEG-SPAKIHAFVDN---NDFGTSYFSSLVNLDKNGVKEMVRYSEFSKFECD 306
S+L AL G S + FV++ D G +F+S V L K+GV + + + +E
Sbjct: 261 MASSVLRALAGESGVEECTFVESPLYKDQGIDFFASRVTLGKDGVDTIHPVGKINDYEES 320
Query: 307 LIEKSLQQLRKDVSKGRKIL 326
L++ +L +L+K ++KG + +
Sbjct: 321 LLKVALGELKKSITKGEQFV 340
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 46.4 bits (105), Expect = 6e-06
Identities = 42/185 (22%), Positives = 75/185 (40%), Gaps = 7/185 (3%)
Query: 16 RSYQVTVVGATSEVGQVXXXXXXXXXXITKLVVHD-NKEFTPGVTLDLSQIPSPSRILGL 74
+S ++ +VGA VG ++V+ D NK+ G +DL+ S +
Sbjct: 19 KSIKIVIVGA-GNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLNHAAPLSHETRV 77
Query: 75 TGEDTLERALKNSDLIIAAGGLSQRPDISEKALLTANSQFIKSIISKLGRSCPIPFVGIV 134
D + K++ ++ G +Q+P + LL AN K I+ ++ + + +
Sbjct: 78 YLGDYKD--CKDATAVVITAGKNQKPGETRMDLLKANISIFKEILREVTKYTKDAILLVA 135
Query: 135 TEPINYLIPMTAEIMRNHGVYDEKKLFGITEIDAIRSQCLYATENNLKANDCYVPVIGGH 194
T P++ L T ++ G E+ + T ID R Q L L +IG H
Sbjct: 136 TNPVDVLTYATLKLT---GFPAERVIGSGTIIDTARFQYLIGKLYGLDPQSVNADIIGEH 192
Query: 195 SDKTI 199
D +
Sbjct: 193 GDSEL 197
>SPAC144.07c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 315
Score = 28.3 bits (60), Expect = 1.6
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 6/68 (8%)
Query: 242 LSVAYSSFLFTKSILDALEGSPAKIHAFVDNNDFGTSYFSSLVNLDKNGVKEMVRYSEFS 301
L + + + L +L P K+ F + D SY + L++ DK + RYS+ +
Sbjct: 163 LDMPHVNILSKADLLCTYGTLPMKLDFFTEVQDL--SYLAPLLDRDKR----LQRYSDLN 216
Query: 302 KFECDLIE 309
K C+L+E
Sbjct: 217 KAICELVE 224
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 26.6 bits (56), Expect = 4.9
Identities = 15/55 (27%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Query: 86 NSDLIIAAGGLSQRPDISEKALLTANSQFIKSIISKLGRSCPIPFVGIVTEPINY 140
N +AA L P+ S + L T ++QF S+ S + P+PF+ + + +
Sbjct: 976 NDPYPVAARFLDAYPEASTQDLNTQDAQFFYSLCSNPFQK-PVPFIPAIDDTFEF 1029
>SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase
I|Schizosaccharomyces pombe|chr 1|||Manual
Length = 859
Score = 26.6 bits (56), Expect = 4.9
Identities = 20/65 (30%), Positives = 31/65 (47%), Gaps = 5/65 (7%)
Query: 156 DEKKLFGITEIDAIRSQCLYATENNLKANDCYVPVIGGHSDKTIVPLLSQAKPTVEMKEK 215
DE++ G+T D + + LY T NL N ++ DK PL + K + KE
Sbjct: 14 DEEESAGLTNKDIVETNHLYPTITNLSLNSDTSSIL---FDKNKKPL--KPKIIIPDKEF 68
Query: 216 DLEEF 220
DL+ +
Sbjct: 69 DLDYY 73
>SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit
Sfc6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 582
Score = 25.8 bits (54), Expect = 8.5
Identities = 10/24 (41%), Positives = 14/24 (58%)
Query: 262 SPAKIHAFVDNNDFGTSYFSSLVN 285
SP K H FV+ DF + SL++
Sbjct: 309 SPVKFHVFVEQADFTFGFNDSLIS 332
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.135 0.377
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,393,225
Number of Sequences: 5004
Number of extensions: 58233
Number of successful extensions: 157
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 151
Number of HSP's gapped (non-prelim): 6
length of query: 329
length of database: 2,362,478
effective HSP length: 73
effective length of query: 256
effective length of database: 1,997,186
effective search space: 511279616
effective search space used: 511279616
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 54 (25.8 bits)
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