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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000991-TA|BGIBMGA000991-PA|IPR004154|Anticodon-binding,
IPR009068|S15/NS1, RNA-binding, IPR010987|Glutathione S-transferase,
C-terminal-like, IPR000738|WHEP-TRS
         (491 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch...   198   1e-51
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    34   0.052
SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb...    31   0.27 
SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr...    29   1.5  
SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1||...    29   1.9  
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha...    29   1.9  
SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe...    27   4.5  
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   4.5  
SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|...    27   4.5  
SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos...    27   4.5  
SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc...    27   5.9  
SPBC19C7.06 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch...    27   5.9  
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo...    27   5.9  
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha...    27   5.9  
SPCC1753.04 |tol1||3'|Schizosaccharomyces pombe|chr 3|||Manual         27   7.8  
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch...    27   7.8  
SPBC776.13 |cnd1||condensin subunit Cnd1|Schizosaccharomyces pom...    27   7.8  
SPAC4D7.04c |||cis-prenyltransferase |Schizosaccharomyces pombe|...    27   7.8  

>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 538

 Score =  198 bits (484), Expect = 1e-51
 Identities = 141/401 (35%), Positives = 200/401 (49%), Gaps = 29/401 (7%)

Query: 1   MADSQEDLLLKIQEQGDLVRKLKAAKDSNERGKGFKEVYNFEEIDKHLSHYSYICGYVPS 60
           MADS  D+  +I  QG+LVR LK+   S E            +IDK ++    +      
Sbjct: 1   MADSLVDINERITSQGNLVRSLKSQGASKE------------DIDKEVAKLLQLKNLKLG 48

Query: 61  KPDVDVAKALKSLDFSNYKYIKRWWNHMRSFSDTEVSLFPD-FRQPNFLKYNS-----KK 114
             +V   K   S      K  K W +      +   S   + F++   +  ++     K+
Sbjct: 49  GSEVSGKKKDTSFTLKTPKGTKDWCDKDVVLREHIFSTVTEIFKRHGGVTLDTPVFELKE 108

Query: 115 ILESQVKEEVSKLLALKAQLVTEDAGPQKFTLKTPKGT-RDYNPQQMTIRNNVLDKIITV 173
           IL  +  E+   +  LK Q    +    ++ L  P       NP+  +I+   + K   V
Sbjct: 109 ILSGKYGEDSKLIYDLKDQ--GGELCSLRYDLTVPFARWLAMNPKVTSIKRFHIAK---V 163

Query: 174 FRRHGAECIDTPVFELK--DFDIAGIYDPMVPDAECLKVVTEILEALDIGQYILKVNHRR 231
           +RR         + E    DFDIAG YDPM+PD+E LKV+ E L+AL++G Y +K+NHR+
Sbjct: 164 YRRDQPAMTKGRMREFYQCDFDIAGNYDPMIPDSEALKVLVEALDALEVGNYTIKLNHRK 223

Query: 232 LLDGMFEACGVPADKFRTTCSTVDKLDKSPWEEVRTEMINEKGVSPDAADRIGEYGRLNG 291
           +LDG+F  CGVP DK RT  S VDKLDK PWE+VR EM+ EKG+  + AD+I EY  L G
Sbjct: 224 ILDGIFTVCGVPDDKIRTISSAVDKLDKLPWEDVRREMVVEKGLKEEVADKIKEYVLLKG 283

Query: 292 STXXXXXXXXXXXXXXXXAALEGVEAIKTLLQYCELFGIKDKILFDLSLARGLDYYTGVI 351
                             +A+     ++ L  Y E FG+ D+  FD+SLARGLDYYTG+I
Sbjct: 284 DRSLLDKLEADSLLSSNSSAVAAFNDMRLLFDYLEAFGVLDRFSFDMSLARGLDYYTGII 343

Query: 352 YEAVLTRVSIGVERIFSVLEAKLAAGDITVRTSDIDVYVAS 392
           YEAV T  S    +I S  E K +A     R++D  + V S
Sbjct: 344 YEAV-TEAS--APKIKSSAEKKKSADPEADRSNDDSIGVGS 381



 Score = 91.9 bits (218), Expect = 2e-19
 Identities = 49/128 (38%), Positives = 75/128 (58%), Gaps = 11/128 (8%)

Query: 359 VSIGVERIFSVLEAKLAAGDITVRTSDIDVYVAS-----AQKNFLEERMKICAELWDAGI 413
           +S+G+ERIFS+L +K+   DI  R +D+DV+V +         FL+ERM +C +LW  GI
Sbjct: 408 ISLGLERIFSILRSKIPDEDI--RANDVDVFVMAFGGGKEWTGFLKERMSVCKDLWANGI 465

Query: 414 KTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDE----VPRN 469
           K E  YK  PK   Q    ++  +P AV+LG  E  +G V+I+ +  +E  +    VPR 
Sbjct: 466 KAEFLYKVKPKPRQQFDAADKFDVPFAVILGQDEFAKGSVRIKQMGLKENSDEGQLVPRE 525

Query: 470 DLVQEIKK 477
           ++V  +KK
Sbjct: 526 EMVSVLKK 533


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 33.9 bits (74), Expect = 0.052
 Identities = 27/106 (25%), Positives = 48/106 (45%), Gaps = 5/106 (4%)

Query: 386  IDVYVASAQKNFLEERMKICAELWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGD 445
            +DV V S  K+ + E+  +  ELW   I+ +   +    +   + H     I   +V+  
Sbjct: 1331 VDVLVTSIGKDSILEKCSLLQELWALNIQADIVLRGASSLEEIVTHYRSEGINWVLVVRQ 1390

Query: 446  SELK-RGVVKIRNITTREEDEVPRND----LVQEIKKRIDNLNLNQ 486
               +    VK RNI   E+DE+  ++    L+ EI +R  N ++ Q
Sbjct: 1391 KNTQMEHSVKARNILKNEDDEIRFDEVGMWLLGEINERKRNESMLQ 1436


>SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 695

 Score = 31.5 bits (68), Expect = 0.27
 Identities = 34/115 (29%), Positives = 54/115 (46%), Gaps = 8/115 (6%)

Query: 36  KEVYNFEEIDKHLSHYSYICGYVPSKPDVDVAKALKSLDFSNYKYI-KRWWNHMRSFSDT 94
           K V+  ++I+ HLS  S +    PS  D+ +    KS  F  +K + ++  N ++S    
Sbjct: 187 KRVFE-DQIEIHLSKSSLLGFNAPSPDDIVLMAQSKSKSFQKHKRLDEQLLNSVKSMKKV 245

Query: 95  EVSLFPDFRQPNFLKYNSKKILESQVK-EEVSKLLALKAQLVTEDAGPQKFTLKT 148
              L P   Q N    N+   L SQ +  E+SKL+  + +L+    GP K   KT
Sbjct: 246 SQQLKP---QKNTNDSNNDHTLLSQDQLIELSKLVKPRTKLLL--LGPPKSGKKT 295


>SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1516

 Score = 29.1 bits (62), Expect = 1.5
 Identities = 15/35 (42%), Positives = 21/35 (60%)

Query: 364 ERIFSVLEAKLAAGDITVRTSDIDVYVASAQKNFL 398
           E IFS+L A L  G+I V+ S  D Y+ S  +N +
Sbjct: 339 ECIFSLLAALLHIGNIEVKHSRNDAYIDSKNENLI 373


>SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 923

 Score = 28.7 bits (61), Expect = 1.9
 Identities = 27/99 (27%), Positives = 41/99 (41%), Gaps = 9/99 (9%)

Query: 16  GDLVRKLKAAKDSNERGKGFKEVYNFEEIDKHLSHYSYICGYVPSKPDVDVAKALKSLDF 75
           G L R L A KD     KG    +  +E    L  Y Y C       D+     +  ++ 
Sbjct: 352 GSLSRYLSAKKDK----KGGDSKFFQKEKTFALQRYDYYC----FMQDLHDGSIINDING 403

Query: 76  SNYKYIKRWWNHMRSFSDTEVSLFPDFRQPNFLKYNSKK 114
              +Y  R ++H+  FS+   S+ P+ +Q N LK    K
Sbjct: 404 IFLQYFHRQYDHIALFSNLMNSVLPNLQQLN-LKLEKTK 441


>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
           Upf1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 925

 Score = 28.7 bits (61), Expect = 1.9
 Identities = 15/61 (24%), Positives = 28/61 (45%)

Query: 388 VYVASAQKNFLEERMKICAELWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSE 447
           + VA+A+K  L     IC     AG +    YK    ++++     E +  + +VLG  +
Sbjct: 525 ILVAAAEKELLRAAHVICCTCVGAGDRRISKYKFRSVLIDEATQASEPECMIPLVLGAKQ 584

Query: 448 L 448
           +
Sbjct: 585 V 585


>SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1465

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 15/67 (22%), Positives = 31/67 (46%)

Query: 74  DFSNYKYIKRWWNHMRSFSDTEVSLFPDFRQPNFLKYNSKKILESQVKEEVSKLLALKAQ 133
           +F++ K  +    H  S+  T++SL P         Y+S +  +S  K +  K   ++ +
Sbjct: 805 EFNDEKDTQPLPEHTTSYPSTQISLAPSIHVEGLETYSSSERKDSSNKYKSRKRNPIRQK 864

Query: 134 LVTEDAG 140
           +  +D G
Sbjct: 865 VTEDDKG 871


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta
          subunit |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)

Query: 42 EEIDKHLSHYSYICGYVPSKPDVDVAKAL-KSLDFSNYKYIKRWWNHMRSF 91
          ++++  L   S+I GY PS+ D  V KA+  + D + Y    RW+  + ++
Sbjct: 13 KQLNDFLLDKSFIEGYEPSQADAVVFKAVGVAPDTAKYPNGARWYKQIATY 63


>SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit
           Prp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 906

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 2/56 (3%)

Query: 437 IPLAVVLGDSELKRGVVKIRNITTREEDEVPRNDL--VQEIKKRIDNLNLNQLNGA 490
           IPL ++L   E K+ V++ R +  R + + P+N+   ++ IK  +   N++Q+  A
Sbjct: 713 IPLWLLLAKLEEKQSVIRARVVFDRAKVKNPKNEFLWLELIKMELRAGNISQVRAA 768


>SPBC11B10.05c |rsp1||random septum position protein
           Rsp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 494

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 16/66 (24%), Positives = 31/66 (46%), Gaps = 2/66 (3%)

Query: 96  VSLFPDFRQPNFLKYNSKKILES--QVKEEVSKLLALKAQLVTEDAGPQKFTLKTPKGTR 153
           +S  P F+ PN+ K+N+K  LES  + +   + L  +   L +        T  +P+ + 
Sbjct: 195 ISSSPRFKGPNYNKFNAKLYLESLREKRRTYTPLSEISNGLNSNGVENSSITKSSPRSSS 254

Query: 154 DYNPQQ 159
             N ++
Sbjct: 255 SSNNER 260


>SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 29/131 (22%), Positives = 58/131 (44%), Gaps = 11/131 (8%)

Query: 350 VIYEAVLTR-VSIGVERIFSVLEAKLAAGDITVRTSDIDVYVASAQ-KNFLEERMKICAE 407
           +++  VL + + + +ER+ + L A      + +  S     +   Q K+ L +      E
Sbjct: 430 LVFRKVLIKDLVLDIERLKNDLNATRKKNGVYLAESTYKELMDRVQNKDLLCQEQARKLE 489

Query: 408 LWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDEVP 467
           + D  +K+ +   +     NQ    E   + L +V   +EL+         + + E+E  
Sbjct: 490 VLDLNVKSSREQLQYVSKSNQEHKKEVEALQLQLVNSSTELE---------SVKSENEKL 540

Query: 468 RNDLVQEIKKR 478
           +N+LV EI+KR
Sbjct: 541 KNELVLEIEKR 551


>SPBC19C7.06 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 716

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 21/88 (23%), Positives = 36/88 (40%), Gaps = 1/88 (1%)

Query: 403 KICAELWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTRE 462
           K+   L  A I+TE   +       +  H E   +PL +  G ++ K+  V      T E
Sbjct: 526 KLADRLNAADIRTEADLRAYTPGY-KFSHWEMKGVPLRLEYGPNDAKKNQVTAVRRDTFE 584

Query: 463 EDEVPRNDLVQEIKKRIDNLNLNQLNGA 490
           +  VP N+L + +   +  +  N    A
Sbjct: 585 KIPVPLNNLEKGVSDLLAKIQTNMYETA 612


>SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1163

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 4/58 (6%)

Query: 425  MLNQLQHCEENKIPLAVVLGDSELKRGV---VKIRNITTREE-DEVPRNDLVQEIKKR 478
            + N  QH E N+  L  V G    +RG+   VK  N   R++ DE      V E+ ++
Sbjct: 1090 LANAQQHAEVNRTYLEAVAGKESFRRGLNNRVKFSNKRVRDDYDEEMEEVEVPEVTRK 1147


>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 4196

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 6/43 (13%)

Query: 422 NPKMLNQLQHCEENKIPLAV------VLGDSELKRGVVKIRNI 458
           +P +L+ ++H EEN + L+V      VL DSE  + +  + N+
Sbjct: 217 HPLILSTIKHAEENNLDLSVRLLPSKVLSDSEFYKSLSNLVNV 259


>SPCC1753.04 |tol1||3'|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 353

 Score = 26.6 bits (56), Expect = 7.8
 Identities = 14/48 (29%), Positives = 23/48 (47%), Gaps = 1/48 (2%)

Query: 387 DVYVASAQKNFLEERMKICAELWDAGIKTEQSYKKNPKMLNQLQHCEE 434
           D  V     +FL E  + C+ +W+   +T Q +    K L Q++  EE
Sbjct: 68  DPIVGEEDSDFLRENTQTCSRVWELVQETIQ-HATEYKELGQIKSAEE 114


>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1318

 Score = 26.6 bits (56), Expect = 7.8
 Identities = 21/72 (29%), Positives = 36/72 (50%), Gaps = 1/72 (1%)

Query: 314  GVEAIKTLLQYCELFGIKDKILFDLSLARGLDYYTGVIYEAVLTRVSIGVERIFSVLEAK 373
            G+E     L+  EL  +++ ILF   L+ G D +  V+   V  R ++G E  F V + +
Sbjct: 955  GIEEKAISLKLNELPFLENWILFQCLLSFGGDDFLKVVAYMVYYRRTVG-EMSFRVSDFE 1013

Query: 374  LAAGDITVRTSD 385
            LA   ++   +D
Sbjct: 1014 LALSRLSTNDAD 1025


>SPBC776.13 |cnd1||condensin subunit Cnd1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1158

 Score = 26.6 bits (56), Expect = 7.8
 Identities = 13/54 (24%), Positives = 26/54 (48%)

Query: 420 KKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDEVPRNDLVQ 473
           K+  K+++     +EN +  A ++ D E   G   + N  +   D VP  ++V+
Sbjct: 460 KQQEKVVDSELEVDENLLEDATMIQDDESHEGESHLENSLSEYVDSVPAEEIVK 513


>SPAC4D7.04c |||cis-prenyltransferase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 264

 Score = 26.6 bits (56), Expect = 7.8
 Identities = 11/27 (40%), Positives = 17/27 (62%)

Query: 314 GVEAIKTLLQYCELFGIKDKILFDLSL 340
           G EA+K+LL+ C   G+K+   F  S+
Sbjct: 63  GFEALKSLLKVCLKLGVKEVSAFTFSI 89


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.136    0.389 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,149,616
Number of Sequences: 5004
Number of extensions: 91638
Number of successful extensions: 277
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 263
Number of HSP's gapped (non-prelim): 24
length of query: 491
length of database: 2,362,478
effective HSP length: 76
effective length of query: 415
effective length of database: 1,982,174
effective search space: 822602210
effective search space used: 822602210
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 56 (26.6 bits)

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