BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000991-TA|BGIBMGA000991-PA|IPR004154|Anticodon-binding, IPR009068|S15/NS1, RNA-binding, IPR010987|Glutathione S-transferase, C-terminal-like, IPR000738|WHEP-TRS (491 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 198 1e-51 SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 34 0.052 SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 31 0.27 SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr... 29 1.5 SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1||... 29 1.9 SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 29 1.9 SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe... 27 4.5 SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 27 4.5 SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|... 27 4.5 SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 27 4.5 SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 27 5.9 SPBC19C7.06 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 27 5.9 SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 27 5.9 SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 27 5.9 SPCC1753.04 |tol1||3'|Schizosaccharomyces pombe|chr 3|||Manual 27 7.8 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 27 7.8 SPBC776.13 |cnd1||condensin subunit Cnd1|Schizosaccharomyces pom... 27 7.8 SPAC4D7.04c |||cis-prenyltransferase |Schizosaccharomyces pombe|... 27 7.8 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 198 bits (484), Expect = 1e-51 Identities = 141/401 (35%), Positives = 200/401 (49%), Gaps = 29/401 (7%) Query: 1 MADSQEDLLLKIQEQGDLVRKLKAAKDSNERGKGFKEVYNFEEIDKHLSHYSYICGYVPS 60 MADS D+ +I QG+LVR LK+ S E +IDK ++ + Sbjct: 1 MADSLVDINERITSQGNLVRSLKSQGASKE------------DIDKEVAKLLQLKNLKLG 48 Query: 61 KPDVDVAKALKSLDFSNYKYIKRWWNHMRSFSDTEVSLFPD-FRQPNFLKYNS-----KK 114 +V K S K K W + + S + F++ + ++ K+ Sbjct: 49 GSEVSGKKKDTSFTLKTPKGTKDWCDKDVVLREHIFSTVTEIFKRHGGVTLDTPVFELKE 108 Query: 115 ILESQVKEEVSKLLALKAQLVTEDAGPQKFTLKTPKGT-RDYNPQQMTIRNNVLDKIITV 173 IL + E+ + LK Q + ++ L P NP+ +I+ + K V Sbjct: 109 ILSGKYGEDSKLIYDLKDQ--GGELCSLRYDLTVPFARWLAMNPKVTSIKRFHIAK---V 163 Query: 174 FRRHGAECIDTPVFELK--DFDIAGIYDPMVPDAECLKVVTEILEALDIGQYILKVNHRR 231 +RR + E DFDIAG YDPM+PD+E LKV+ E L+AL++G Y +K+NHR+ Sbjct: 164 YRRDQPAMTKGRMREFYQCDFDIAGNYDPMIPDSEALKVLVEALDALEVGNYTIKLNHRK 223 Query: 232 LLDGMFEACGVPADKFRTTCSTVDKLDKSPWEEVRTEMINEKGVSPDAADRIGEYGRLNG 291 +LDG+F CGVP DK RT S VDKLDK PWE+VR EM+ EKG+ + AD+I EY L G Sbjct: 224 ILDGIFTVCGVPDDKIRTISSAVDKLDKLPWEDVRREMVVEKGLKEEVADKIKEYVLLKG 283 Query: 292 STXXXXXXXXXXXXXXXXAALEGVEAIKTLLQYCELFGIKDKILFDLSLARGLDYYTGVI 351 +A+ ++ L Y E FG+ D+ FD+SLARGLDYYTG+I Sbjct: 284 DRSLLDKLEADSLLSSNSSAVAAFNDMRLLFDYLEAFGVLDRFSFDMSLARGLDYYTGII 343 Query: 352 YEAVLTRVSIGVERIFSVLEAKLAAGDITVRTSDIDVYVAS 392 YEAV T S +I S E K +A R++D + V S Sbjct: 344 YEAV-TEAS--APKIKSSAEKKKSADPEADRSNDDSIGVGS 381 Score = 91.9 bits (218), Expect = 2e-19 Identities = 49/128 (38%), Positives = 75/128 (58%), Gaps = 11/128 (8%) Query: 359 VSIGVERIFSVLEAKLAAGDITVRTSDIDVYVAS-----AQKNFLEERMKICAELWDAGI 413 +S+G+ERIFS+L +K+ DI R +D+DV+V + FL+ERM +C +LW GI Sbjct: 408 ISLGLERIFSILRSKIPDEDI--RANDVDVFVMAFGGGKEWTGFLKERMSVCKDLWANGI 465 Query: 414 KTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDE----VPRN 469 K E YK PK Q ++ +P AV+LG E +G V+I+ + +E + VPR Sbjct: 466 KAEFLYKVKPKPRQQFDAADKFDVPFAVILGQDEFAKGSVRIKQMGLKENSDEGQLVPRE 525 Query: 470 DLVQEIKK 477 ++V +KK Sbjct: 526 EMVSVLKK 533 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 33.9 bits (74), Expect = 0.052 Identities = 27/106 (25%), Positives = 48/106 (45%), Gaps = 5/106 (4%) Query: 386 IDVYVASAQKNFLEERMKICAELWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGD 445 +DV V S K+ + E+ + ELW I+ + + + + H I +V+ Sbjct: 1331 VDVLVTSIGKDSILEKCSLLQELWALNIQADIVLRGASSLEEIVTHYRSEGINWVLVVRQ 1390 Query: 446 SELK-RGVVKIRNITTREEDEVPRND----LVQEIKKRIDNLNLNQ 486 + VK RNI E+DE+ ++ L+ EI +R N ++ Q Sbjct: 1391 KNTQMEHSVKARNILKNEDDEIRFDEVGMWLLGEINERKRNESMLQ 1436 >SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 695 Score = 31.5 bits (68), Expect = 0.27 Identities = 34/115 (29%), Positives = 54/115 (46%), Gaps = 8/115 (6%) Query: 36 KEVYNFEEIDKHLSHYSYICGYVPSKPDVDVAKALKSLDFSNYKYI-KRWWNHMRSFSDT 94 K V+ ++I+ HLS S + PS D+ + KS F +K + ++ N ++S Sbjct: 187 KRVFE-DQIEIHLSKSSLLGFNAPSPDDIVLMAQSKSKSFQKHKRLDEQLLNSVKSMKKV 245 Query: 95 EVSLFPDFRQPNFLKYNSKKILESQVK-EEVSKLLALKAQLVTEDAGPQKFTLKT 148 L P Q N N+ L SQ + E+SKL+ + +L+ GP K KT Sbjct: 246 SQQLKP---QKNTNDSNNDHTLLSQDQLIELSKLVKPRTKLLL--LGPPKSGKKT 295 >SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr 3|||Manual Length = 1516 Score = 29.1 bits (62), Expect = 1.5 Identities = 15/35 (42%), Positives = 21/35 (60%) Query: 364 ERIFSVLEAKLAAGDITVRTSDIDVYVASAQKNFL 398 E IFS+L A L G+I V+ S D Y+ S +N + Sbjct: 339 ECIFSLLAALLHIGNIEVKHSRNDAYIDSKNENLI 373 >SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 923 Score = 28.7 bits (61), Expect = 1.9 Identities = 27/99 (27%), Positives = 41/99 (41%), Gaps = 9/99 (9%) Query: 16 GDLVRKLKAAKDSNERGKGFKEVYNFEEIDKHLSHYSYICGYVPSKPDVDVAKALKSLDF 75 G L R L A KD KG + +E L Y Y C D+ + ++ Sbjct: 352 GSLSRYLSAKKDK----KGGDSKFFQKEKTFALQRYDYYC----FMQDLHDGSIINDING 403 Query: 76 SNYKYIKRWWNHMRSFSDTEVSLFPDFRQPNFLKYNSKK 114 +Y R ++H+ FS+ S+ P+ +Q N LK K Sbjct: 404 IFLQYFHRQYDHIALFSNLMNSVLPNLQQLN-LKLEKTK 441 >SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 925 Score = 28.7 bits (61), Expect = 1.9 Identities = 15/61 (24%), Positives = 28/61 (45%) Query: 388 VYVASAQKNFLEERMKICAELWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSE 447 + VA+A+K L IC AG + YK ++++ E + + +VLG + Sbjct: 525 ILVAAAEKELLRAAHVICCTCVGAGDRRISKYKFRSVLIDEATQASEPECMIPLVLGAKQ 584 Query: 448 L 448 + Sbjct: 585 V 585 >SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 1465 Score = 27.5 bits (58), Expect = 4.5 Identities = 15/67 (22%), Positives = 31/67 (46%) Query: 74 DFSNYKYIKRWWNHMRSFSDTEVSLFPDFRQPNFLKYNSKKILESQVKEEVSKLLALKAQ 133 +F++ K + H S+ T++SL P Y+S + +S K + K ++ + Sbjct: 805 EFNDEKDTQPLPEHTTSYPSTQISLAPSIHVEGLETYSSSERKDSSNKYKSRKRNPIRQK 864 Query: 134 LVTEDAG 140 + +D G Sbjct: 865 VTEDDKG 871 >SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 214 Score = 27.5 bits (58), Expect = 4.5 Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Query: 42 EEIDKHLSHYSYICGYVPSKPDVDVAKAL-KSLDFSNYKYIKRWWNHMRSF 91 ++++ L S+I GY PS+ D V KA+ + D + Y RW+ + ++ Sbjct: 13 KQLNDFLLDKSFIEGYEPSQADAVVFKAVGVAPDTAKYPNGARWYKQIATY 63 >SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 906 Score = 27.5 bits (58), Expect = 4.5 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 2/56 (3%) Query: 437 IPLAVVLGDSELKRGVVKIRNITTREEDEVPRNDL--VQEIKKRIDNLNLNQLNGA 490 IPL ++L E K+ V++ R + R + + P+N+ ++ IK + N++Q+ A Sbjct: 713 IPLWLLLAKLEEKQSVIRARVVFDRAKVKNPKNEFLWLELIKMELRAGNISQVRAA 768 >SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 494 Score = 27.5 bits (58), Expect = 4.5 Identities = 16/66 (24%), Positives = 31/66 (46%), Gaps = 2/66 (3%) Query: 96 VSLFPDFRQPNFLKYNSKKILES--QVKEEVSKLLALKAQLVTEDAGPQKFTLKTPKGTR 153 +S P F+ PN+ K+N+K LES + + + L + L + T +P+ + Sbjct: 195 ISSSPRFKGPNYNKFNAKLYLESLREKRRTYTPLSEISNGLNSNGVENSSITKSSPRSSS 254 Query: 154 DYNPQQ 159 N ++ Sbjct: 255 SSNNER 260 >SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 27.1 bits (57), Expect = 5.9 Identities = 29/131 (22%), Positives = 58/131 (44%), Gaps = 11/131 (8%) Query: 350 VIYEAVLTR-VSIGVERIFSVLEAKLAAGDITVRTSDIDVYVASAQ-KNFLEERMKICAE 407 +++ VL + + + +ER+ + L A + + S + Q K+ L + E Sbjct: 430 LVFRKVLIKDLVLDIERLKNDLNATRKKNGVYLAESTYKELMDRVQNKDLLCQEQARKLE 489 Query: 408 LWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDEVP 467 + D +K+ + + NQ E + L +V +EL+ + + E+E Sbjct: 490 VLDLNVKSSREQLQYVSKSNQEHKKEVEALQLQLVNSSTELE---------SVKSENEKL 540 Query: 468 RNDLVQEIKKR 478 +N+LV EI+KR Sbjct: 541 KNELVLEIEKR 551 >SPBC19C7.06 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 716 Score = 27.1 bits (57), Expect = 5.9 Identities = 21/88 (23%), Positives = 36/88 (40%), Gaps = 1/88 (1%) Query: 403 KICAELWDAGIKTEQSYKKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTRE 462 K+ L A I+TE + + H E +PL + G ++ K+ V T E Sbjct: 526 KLADRLNAADIRTEADLRAYTPGY-KFSHWEMKGVPLRLEYGPNDAKKNQVTAVRRDTFE 584 Query: 463 EDEVPRNDLVQEIKKRIDNLNLNQLNGA 490 + VP N+L + + + + N A Sbjct: 585 KIPVPLNNLEKGVSDLLAKIQTNMYETA 612 >SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1163 Score = 27.1 bits (57), Expect = 5.9 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 4/58 (6%) Query: 425 MLNQLQHCEENKIPLAVVLGDSELKRGV---VKIRNITTREE-DEVPRNDLVQEIKKR 478 + N QH E N+ L V G +RG+ VK N R++ DE V E+ ++ Sbjct: 1090 LANAQQHAEVNRTYLEAVAGKESFRRGLNNRVKFSNKRVRDDYDEEMEEVEVPEVTRK 1147 >SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 4196 Score = 27.1 bits (57), Expect = 5.9 Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 6/43 (13%) Query: 422 NPKMLNQLQHCEENKIPLAV------VLGDSELKRGVVKIRNI 458 +P +L+ ++H EEN + L+V VL DSE + + + N+ Sbjct: 217 HPLILSTIKHAEENNLDLSVRLLPSKVLSDSEFYKSLSNLVNV 259 >SPCC1753.04 |tol1||3'|Schizosaccharomyces pombe|chr 3|||Manual Length = 353 Score = 26.6 bits (56), Expect = 7.8 Identities = 14/48 (29%), Positives = 23/48 (47%), Gaps = 1/48 (2%) Query: 387 DVYVASAQKNFLEERMKICAELWDAGIKTEQSYKKNPKMLNQLQHCEE 434 D V +FL E + C+ +W+ +T Q + K L Q++ EE Sbjct: 68 DPIVGEEDSDFLRENTQTCSRVWELVQETIQ-HATEYKELGQIKSAEE 114 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 26.6 bits (56), Expect = 7.8 Identities = 21/72 (29%), Positives = 36/72 (50%), Gaps = 1/72 (1%) Query: 314 GVEAIKTLLQYCELFGIKDKILFDLSLARGLDYYTGVIYEAVLTRVSIGVERIFSVLEAK 373 G+E L+ EL +++ ILF L+ G D + V+ V R ++G E F V + + Sbjct: 955 GIEEKAISLKLNELPFLENWILFQCLLSFGGDDFLKVVAYMVYYRRTVG-EMSFRVSDFE 1013 Query: 374 LAAGDITVRTSD 385 LA ++ +D Sbjct: 1014 LALSRLSTNDAD 1025 >SPBC776.13 |cnd1||condensin subunit Cnd1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1158 Score = 26.6 bits (56), Expect = 7.8 Identities = 13/54 (24%), Positives = 26/54 (48%) Query: 420 KKNPKMLNQLQHCEENKIPLAVVLGDSELKRGVVKIRNITTREEDEVPRNDLVQ 473 K+ K+++ +EN + A ++ D E G + N + D VP ++V+ Sbjct: 460 KQQEKVVDSELEVDENLLEDATMIQDDESHEGESHLENSLSEYVDSVPAEEIVK 513 >SPAC4D7.04c |||cis-prenyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 264 Score = 26.6 bits (56), Expect = 7.8 Identities = 11/27 (40%), Positives = 17/27 (62%) Query: 314 GVEAIKTLLQYCELFGIKDKILFDLSL 340 G EA+K+LL+ C G+K+ F S+ Sbjct: 63 GFEALKSLLKVCLKLGVKEVSAFTFSI 89 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.136 0.389 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,149,616 Number of Sequences: 5004 Number of extensions: 91638 Number of successful extensions: 277 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 263 Number of HSP's gapped (non-prelim): 24 length of query: 491 length of database: 2,362,478 effective HSP length: 76 effective length of query: 415 effective length of database: 1,982,174 effective search space: 822602210 effective search space used: 822602210 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 56 (26.6 bits)
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