Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000990-TA|BGIBMGA000990-PA|IPR012337|Polynucleotidyl
transferase, Ribonuclease H fold
         (290 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g25910.1 68415.m03109 3'-5' exonuclease domain-containing pro...    46   4e-05
At2g40210.1 68415.m04945 MADS-box family protein contains Pfam p...    35   0.073
At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--t...    30   2.1  
At3g13000.2 68416.m01620 expressed protein contains Pfam profile...    29   2.8  
At3g13000.1 68416.m01619 expressed protein contains Pfam profile...    29   2.8  
At1g05320.1 68414.m00539 myosin-related similar to non-muscle my...    29   2.8  
At1g18410.1 68414.m02299 kinesin motor protein-related similar t...    28   8.4  

>At2g25910.1 68415.m03109 3'-5' exonuclease domain-containing
           protein / K homology domain-containing protein / KH
           domain-containing protein contains Pfam profiles
           PF01612: 3'-5' exonuclease, PF00013: KH domain
          Length = 341

 Score = 45.6 bits (103), Expect = 4e-05
 Identities = 22/87 (25%), Positives = 46/87 (52%), Gaps = 1/87 (1%)

Query: 120 DGANMGRKCKMPFLVLSTDHQIYIFDIQVMQYHAFESGLKKILEGDSPKKIAHDCRKLSD 179
           +G ++ R  K+  + ++  + IY+ D+ +          K  LE +   K+ HDC++ S+
Sbjct: 63  EGVDLCRHGKLCIMQIAFSNAIYLVDV-IEGGEVIMKACKPALESNYITKVIHDCKRDSE 121

Query: 180 CLYHKHNVKLKSVFDTQVGDLIITKNK 206
            LY +  ++L +V DTQ+   +I + +
Sbjct: 122 ALYFQFGIRLHNVVDTQIAYSLIEEQE 148


>At2g40210.1 68415.m04945 MADS-box family protein contains Pfam
           profile: PF00319 SRF-type transcription factor
           (DNA-binding and dimerisation domain)
          Length = 371

 Score = 34.7 bits (76), Expect = 0.073
 Identities = 34/118 (28%), Positives = 57/118 (48%), Gaps = 11/118 (9%)

Query: 44  PHGDANDGVLHYYDSEIREVVKLQESTEKKVLKISQTKYEEILKISKKYIFINQVDKSFH 103
           P   A  G+L  + +  + V K +ES  +  LK    K++E LK SKK    + +D+   
Sbjct: 59  PSPQATHGLLDEFFALPKSVQKKKESNVESYLKEKTHKFQEQLKKSKKKNKEHVIDELMM 118

Query: 104 E-----AVDDLNQQDFIAV---SGDGANMGRKCKMPFLVLS--TDHQIYIFDIQVMQY 151
           +      + DLNQ +  A+   S D   + RK K+ F+      D  ++ F+IQV ++
Sbjct: 119 QLQSGREIADLNQSEMYALLSFSRDTILLCRK-KLAFMQFPPLRDPPVFPFEIQVEEF 175


>At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--tRNA
           ligase identical to histidyl-tRNA synthetase
           [Arabidopsis thaliana] GI:3659909
          Length = 486

 Score = 29.9 bits (64), Expect = 2.1
 Identities = 28/123 (22%), Positives = 54/123 (43%), Gaps = 5/123 (4%)

Query: 147 QVMQYHAFESGLKKILEGDSP--KKIAHDCRKLSDCLYHKHN--VKLKSVFDTQVGDLII 202
           +V + + +E     +LE ++   +K   + R    C   + N  V L+      +  L+I
Sbjct: 95  EVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVI 154

Query: 203 TKNKKVTLPNKVRSLGECLTNYLGLQQNTIDEKLKGLLHLAKFSRLNPEANLEASGYTVL 262
            K K V+LP K  ++G+C   Y  + +    E  +  + +    ++  EA L +S  T  
Sbjct: 155 QKGKSVSLPLKWFAIGQC-WRYERMTRGRRREHYQWNMDIIGVPQVTAEAELISSIVTFF 213

Query: 263 KSV 265
           K +
Sbjct: 214 KRI 216


>At3g13000.2 68416.m01620 expressed protein contains Pfam profile
           PF04784: Protein of unknown function, DUF547
          Length = 582

 Score = 29.5 bits (63), Expect = 2.8
 Identities = 14/49 (28%), Positives = 23/49 (46%)

Query: 67  QESTEKKVLKISQTKYEEILKISKKYIFINQVDKSFHEAVDDLNQQDFI 115
           QE  E+++ +   T     L  S    ++NQ D   H++ +D   QD I
Sbjct: 124 QERNERRLAEYQLTHSASPLNSSSSLRYLNQSDSELHQSAEDSPSQDQI 172


>At3g13000.1 68416.m01619 expressed protein contains Pfam profile
           PF04784: Protein of unknown function, DUF547
          Length = 553

 Score = 29.5 bits (63), Expect = 2.8
 Identities = 14/49 (28%), Positives = 23/49 (46%)

Query: 67  QESTEKKVLKISQTKYEEILKISKKYIFINQVDKSFHEAVDDLNQQDFI 115
           QE  E+++ +   T     L  S    ++NQ D   H++ +D   QD I
Sbjct: 95  QERNERRLAEYQLTHSASPLNSSSSLRYLNQSDSELHQSAEDSPSQDQI 143


>At1g05320.1 68414.m00539 myosin-related similar to non-muscle
           myosin II heavy chain (GI:19879404) [Loligo pealei];
           ESTs gb|AA042402,gb|ATTS1380 come from this gene
          Length = 828

 Score = 29.5 bits (63), Expect = 2.8
 Identities = 33/148 (22%), Positives = 65/148 (43%), Gaps = 12/148 (8%)

Query: 134 VLSTDHQIYIFDIQVMQYHAFESGLKKILEGDSPKKIAHDCRKLSDCLY-----HKHNVK 188
           V+  + +  IFD   ++    + G+ K  + D  +K     + L D L      HK   +
Sbjct: 23  VMEKEEEDTIFDGGFVKVE--KEGINKKYDDDDDEKAEKQLKSLEDALQLHDVKHKELTE 80

Query: 189 LKSVFDTQVGDLIITKNKKVTLPNKVRSL---GECLTNYLGLQQNTIDEKLKGLLHLAKF 245
           +K  FD    +L  ++ K + L +++R      E L        + ++EKLK  +   ++
Sbjct: 81  VKEAFDGLGLELENSRKKMIELEDRIRISALEAEKLEELQKQSASELEEKLK--ISDERY 138

Query: 246 SRLNPEANLEASGYTVLKSVTESIVSLS 273
           S+ +   +   S  +VL+   +S+  LS
Sbjct: 139 SKTDALLSQALSQNSVLEQKLKSLEELS 166


>At1g18410.1 68414.m02299 kinesin motor protein-related similar to
           kinesin-related protein GB:AAF24855 GI:6692749 from
           [Arabidopsis thaliana]
          Length = 1140

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 17/75 (22%), Positives = 39/75 (52%), Gaps = 2/75 (2%)

Query: 60  IREVVKLQESTEKKVLKISQTKYEEILKISKKYIFINQVDKSFHEAVDDLNQQDFIAVSG 119
           ++E+    +S ++++LK+      E  ++ KK + +     ++HE + + NQ+ F  +  
Sbjct: 570 LQELRFYSKSIKQEILKVQDKYTVEFSQLGKKLLELGDAAANYHEVLTE-NQKLFNELQE 628

Query: 120 DGANMGRKCKM-PFL 133
              N+   C++ PFL
Sbjct: 629 LKGNIRVYCRVRPFL 643


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.317    0.135    0.378 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,692,547
Number of Sequences: 28952
Number of extensions: 271884
Number of successful extensions: 796
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 792
Number of HSP's gapped (non-prelim): 8
length of query: 290
length of database: 12,070,560
effective HSP length: 80
effective length of query: 210
effective length of database: 9,754,400
effective search space: 2048424000
effective search space used: 2048424000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 59 (27.9 bits)