BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000983-TA|BGIBMGA000983-PA|IPR002290|Serine/threonine protein kinase, IPR008271|Serine/threonine protein kinase, active site, IPR003280|K+ channel, two pore, IPR000719|Protein kinase, IPR011009|Protein kinase-like, IPR013099|Ion transport 2 (559 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 180 4e-47 AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 111 1e-26 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 87 3e-19 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 68 2e-13 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 68 2e-13 AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 64 2e-12 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 54 4e-09 DQ288391-1|ABC41341.1| 630|Apis mellifera vasa protein protein. 26 0.71 AY855337-1|AAW47987.1| 510|Apis mellifera tyrosine hydroxylase ... 23 5.0 AY331183-1|AAP94623.1| 953|Apis mellifera NMDA-type glutamate r... 23 6.7 L10430-1|AAA27731.1| 150|Apis mellifera transposase protein. 23 8.8 DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 23 8.8 DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 23 8.8 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 180 bits (437), Expect = 4e-47 Identities = 81/184 (44%), Positives = 122/184 (66%), Gaps = 4/184 (2%) Query: 6 TCKDNMYVYFILPYEAGGELYTLIKKLGGFSEPLSQFYAAQLVLALEYLHHCSVVHRDLK 65 T KD Y+Y ++ GGEL+T+++ G F + ++FY A +V A +YLH ++++RDLK Sbjct: 434 TFKDRKYLYMLMEACLGGELWTVLRDKGHFDDGTTRFYTACVVEAFDYLHSRNIIYRDLK 493 Query: 66 PENVLVSSSGYIKLADFGFCKIIK--SRTWTLCGTPEYLAPELILSKGYSFPVDWWALGV 123 PEN+L+ S GY+KL DFGF K + +TWT CGTPEY+APE+IL+KG+ D+W+LGV Sbjct: 494 PENLLLDSQGYVKLVDFGFAKRLDHGRKTWTFCGTPEYVAPEVILNKGHDISADYWSLGV 553 Query: 124 LIYEMIAAFPPFYSSEPIKLYEKILTG--QFKTPENMTQVCKALVKNLLEVDPTKRYGSL 181 L++E++ PPF +P+K Y IL G + P ++T+ AL+K L +P +R G Sbjct: 554 LMFELLTGTPPFTGGDPMKTYNIILKGIDAIEFPRSITRNATALIKKLCRDNPAERLGYQ 613 Query: 182 KAGV 185 K G+ Sbjct: 614 KGGI 617 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 111 bits (268), Expect = 1e-26 Identities = 58/159 (36%), Positives = 95/159 (59%), Gaps = 10/159 (6%) Query: 35 FSEPLSQFYAAQLVLALEYLHHCSVVHRDLKPENVLVSSSGY---IKLADFGFCKIIK-- 89 +SE + Q++ ++ + HH VVHRDLKPEN+L++S +KLADFG ++ Sbjct: 6 YSEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGE 65 Query: 90 SRTWT-LCGTPEYLAPELILSKGYSFPVDWWALGVLIYEMIAAFPPFYSSEPIKLYEKIL 148 ++ W GTP YL+PE++ + Y PVD WA GV++Y ++ +PPF+ + +LY +I Sbjct: 66 AQAWFGFAGTPGYLSPEVLKKEPYGKPVDIWACGVILYILLVGYPPFWDEDQHRLYAQIK 125 Query: 149 TGQFKTP----ENMTQVCKALVKNLLEVDPTKRYGSLKA 183 TG + P + +T K L+ +L V+P+KR + +A Sbjct: 126 TGSYDYPSPEWDTVTPEAKNLINQMLTVNPSKRITASEA 164 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 87.4 bits (207), Expect = 3e-19 Identities = 41/90 (45%), Positives = 61/90 (67%), Gaps = 3/90 (3%) Query: 13 VYFILPYEAGGELYTLIKKLGGFSEPLSQFYAAQLVLALEYLHHCSVVHRDLKPENVLVS 72 +YF++ Y GG+L I++ G F EP++ FYA+++ + L +LH +V+RDLK +NVL+ Sbjct: 60 LYFVMEYVNGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVLLD 119 Query: 73 SSGYIKLADFGFCK--IIKSRTW-TLCGTP 99 G+IK+ADFG CK I +T T CGTP Sbjct: 120 QDGHIKIADFGMCKEGISGDKTTKTFCGTP 149 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 68.1 bits (159), Expect = 2e-13 Identities = 54/177 (30%), Positives = 84/177 (47%), Gaps = 14/177 (7%) Query: 44 AAQLVLALEYLHHCSVVHRDLKPENVLVSSSGYIKLADFGFCKIIKSRTWTLCGTPEYLA 103 A ++ + YLH +VHRD+K +NVL+ KL DFGFC ++ GTP ++A Sbjct: 703 ALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCITEVMMLGSIVGTPVHMA 762 Query: 104 PELILSKGYSFPVDWWALGVLIYEMIA-------AFPPFYSSEPI-KLYEKILTGQFKTP 155 PEL LS Y VD +A G+L + + A F F++ E + +K L P Sbjct: 763 PEL-LSGHYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWTSVKKALMIVGIRP 821 Query: 156 ENMTQV---CKALVKNLLEVDPTKRYGSLKAGVFRALEYPAEMAKQDFHRERVLSER 209 E + C L++ +P+KR L + LE + AK+ + V S++ Sbjct: 822 ERLPSFDDECWRLMEQCWSGEPSKR--PLLGAIVPVLESIQQKAKRSKSLQEVSSDK 876 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 68.1 bits (159), Expect = 2e-13 Identities = 54/177 (30%), Positives = 84/177 (47%), Gaps = 14/177 (7%) Query: 44 AAQLVLALEYLHHCSVVHRDLKPENVLVSSSGYIKLADFGFCKIIKSRTWTLCGTPEYLA 103 A ++ + YLH +VHRD+K +NVL+ KL DFGFC ++ GTP ++A Sbjct: 741 ALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCITEVMMLGSIVGTPVHMA 800 Query: 104 PELILSKGYSFPVDWWALGVLIYEMIA-------AFPPFYSSEPI-KLYEKILTGQFKTP 155 PEL LS Y VD +A G+L + + A F F++ E + +K L P Sbjct: 801 PEL-LSGHYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWTSVKKALMIVGIRP 859 Query: 156 ENMTQV---CKALVKNLLEVDPTKRYGSLKAGVFRALEYPAEMAKQDFHRERVLSER 209 E + C L++ +P+KR L + LE + AK+ + V S++ Sbjct: 860 ERLPSFDDECWRLMEQCWSGEPSKR--PLLGAIVPVLESIQQKAKRSKSLQEVSSDK 914 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 64.5 bits (150), Expect = 2e-12 Identities = 32/91 (35%), Positives = 53/91 (58%), Gaps = 2/91 (2%) Query: 47 LVLALEYLHHCSVVHRDLKPENVLVSSSGYIKLADFGFCKII--KSRTWTLCGTPEYLAP 104 + AL++ H+ +VH D+KP+N+L+S +G KL DFG +I + GTP Y AP Sbjct: 164 ITCALQFCHNAGIVHADVKPKNILMSKNGQPKLTDFGSSVLIGAPNEIDKFYGTPGYTAP 223 Query: 105 ELILSKGYSFPVDWWALGVLIYEMIAAFPPF 135 E+I + D ++LG++ ++M+ PF Sbjct: 224 EVIKQNRPTPAADIYSLGIVAWQMLFRKLPF 254 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 53.6 bits (123), Expect = 4e-09 Identities = 36/125 (28%), Positives = 64/125 (51%), Gaps = 7/125 (5%) Query: 13 VYFILPYEAGGELYTLIKKLGGFSEPLSQFYAAQ-LVLALEYLHHCSVVHRDLKPENVLV 71 V I + G L T ++ G + L + + ++YL + VHRDL NVLV Sbjct: 709 VMIITEFMENGSLDTFLRANDGKFQVLQLVGMLRGIASGMQYLAEMNYVHRDLAARNVLV 768 Query: 72 SSSGYIKLADFGFCKIIKSRT---WTLCG---TPEYLAPELILSKGYSFPVDWWALGVLI 125 +++ K+ADFG + I+S T +T G + APE I + ++ D W++G++ Sbjct: 769 NAALVCKIADFGLSREIESATEGAYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSMGIVC 828 Query: 126 YEMIA 130 +E+++ Sbjct: 829 WEVMS 833 >DQ288391-1|ABC41341.1| 630|Apis mellifera vasa protein protein. Length = 630 Score = 26.2 bits (55), Expect = 0.71 Identities = 16/66 (24%), Positives = 28/66 (42%) Query: 263 TEERWSILQAVFFSSTVLTTIGYGNIVPVTFWGRLFCIAYALIGIPFTLTVIADLGRVFA 322 T + W + +S + T + YG + G+L + L+ P L + GRV Sbjct: 286 TIQIWQQIVKFSLNSILKTVVAYGGTSVMHQRGKLSAGCHILVATPGRLLDFVEKGRVKF 345 Query: 323 TCVSFI 328 + V F+ Sbjct: 346 SSVQFL 351 >AY855337-1|AAW47987.1| 510|Apis mellifera tyrosine hydroxylase protein. Length = 510 Score = 23.4 bits (48), Expect = 5.0 Identities = 11/29 (37%), Positives = 15/29 (51%) Query: 303 ALIGIPFTLTVIADLGRVFATCVSFIAKH 331 A+ +P+T T RVF T V + KH Sbjct: 226 AIPTVPYTETETETWTRVFNTLVDLVPKH 254 >AY331183-1|AAP94623.1| 953|Apis mellifera NMDA-type glutamate receptor 1 protein. Length = 953 Score = 23.0 bits (47), Expect = 6.7 Identities = 14/54 (25%), Positives = 26/54 (48%) Query: 264 EERWSILQAVFFSSTVLTTIGYGNIVPVTFWGRLFCIAYALIGIPFTLTVIADL 317 E+ ++ AV+F+ VL G G P +F R+ + +A + + A+L Sbjct: 605 EDALNLSSAVWFAWGVLLNSGIGEGTPRSFSARVLGMVWAGFAMIIVASYTANL 658 >L10430-1|AAA27731.1| 150|Apis mellifera transposase protein. Length = 150 Score = 22.6 bits (46), Expect = 8.8 Identities = 11/31 (35%), Positives = 18/31 (58%) Query: 48 VLALEYLHHCSVVHRDLKPENVLVSSSGYIK 78 VL L + H +V+ +L P N ++S YI+ Sbjct: 67 VLLLVWWDHKGIVYFELLPPNRTINSVVYIE 97 >DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 22.6 bits (46), Expect = 8.8 Identities = 9/22 (40%), Positives = 14/22 (63%) Query: 527 ELEAAVEAVIRDITSPVQQKPP 548 +L + EA I ++ SPV + PP Sbjct: 452 DLSVSGEAGIEEVKSPVLRSPP 473 >DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 22.6 bits (46), Expect = 8.8 Identities = 9/22 (40%), Positives = 14/22 (63%) Query: 527 ELEAAVEAVIRDITSPVQQKPP 548 +L + EA I ++ SPV + PP Sbjct: 452 DLSVSGEAGIEEVKSPVLRSPP 473 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.323 0.139 0.427 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 163,337 Number of Sequences: 429 Number of extensions: 6640 Number of successful extensions: 36 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 15 Number of HSP's gapped (non-prelim): 15 length of query: 559 length of database: 140,377 effective HSP length: 61 effective length of query: 498 effective length of database: 114,208 effective search space: 56875584 effective search space used: 56875584 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (22.0 bits) S2: 46 (22.6 bits)
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