SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000982-TA|BGIBMGA000982-PA|IPR002290|Serine/threonine
protein kinase, IPR000961|Protein kinase, C-terminal,
IPR008271|Serine/threonine protein kinase, active site,
IPR000719|Protein kinase, IPR011009|Protein kinase-like
         (330 letters)

Database: bee 
           429 sequences; 140,377 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF469010-1|AAL93136.1|  678|Apis mellifera cGMP-dependent protei...   251   7e-69
AB013287-1|BAA87893.1|  190|Apis mellifera calmodulin kinase II ...   108   8e-26
AB013288-1|BAA87894.1|  149|Apis mellifera protein kinase C prot...   107   1e-25
DQ013068-1|AAY81956.1|  931|Apis mellifera dusty protein kinase ...    72   8e-15
DQ013067-1|AAY81955.1|  969|Apis mellifera dusty protein kinase ...    72   8e-15
AB183889-1|BAD86829.1|  316|Apis mellifera Mos protein.                64   2e-12
AY921579-1|AAX14899.1|  996|Apis mellifera ephrin receptor protein.    50   4e-08
AB072429-1|BAB83990.1|  388|Apis mellifera IP3phosphatase protein.     22   8.5  

>AF469010-1|AAL93136.1|  678|Apis mellifera cGMP-dependent protein
           kinase foraging protein.
          Length = 678

 Score =  251 bits (614), Expect = 7e-69
 Identities = 123/300 (41%), Positives = 184/300 (61%), Gaps = 5/300 (1%)

Query: 17  DKTVDDFDIVKAIGNGAYGEVFLVRDK-ITFTYHAMKVVEKSVVVERNHAKHLILEKKIL 75
           D  + D   +  +G G +G V LV+    +    A+K ++K+ +VE    +H++ EK+I+
Sbjct: 361 DLRLQDLRPLATLGVGGFGRVELVQIAGDSSRSFALKQMKKAQIVETRQQQHIMSEKRIM 420

Query: 76  QCIQFPFVVTLDVAFKDNLYLYFILPYIAGGEMFTYIQKYGIFSDSLTKFYASQVILALE 135
                 FVV L   FKD  YLY ++    GGE++T ++  G F D  T+FY + V+ A +
Sbjct: 421 GEADCDFVVKLFKTFKDRKYLYMLMEACLGGELWTVLRDKGHFDDGTTRFYTACVVEAFD 480

Query: 136 YLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVLK--KKTWTLCGTPEYLAPEVITSK 193
           YLH   +++RD+KPEN+L+DS GY+KL DFGF K L   +KTWT CGTPEY+APEVI +K
Sbjct: 481 YLHSRNIIYRDLKPENLLLDSQGYVKLVDFGFAKRLDHGRKTWTFCGTPEYVAPEVILNK 540

Query: 194 GYSFSVDWWALGDLSFEMEAGHPPFFASDPNKLYEKVLVG--SYKCPENMSPECKNLLKG 251
           G+  S D+W+LG L FE+  G PPF   DP K Y  +L G  + + P +++     L+K 
Sbjct: 541 GHDISADYWSLGVLMFELLTGTPPFTGGDPMKTYNIILKGIDAIEFPRSITRNATALIKK 600

Query: 252 LLQVDPSKRIGSLKGGIYEIKSHTWFNGLDWQAIFHQKLIAPFVPICSSPGDTSNFPEIP 311
           L + +P++R+G  KGGI EI+ H WF+G +W+ +  + L  P +P   +  DT+NF E P
Sbjct: 601 LCRDNPAERLGYQKGGISEIQKHKWFDGFNWEGLRARTLEPPIMPRVQNATDTTNFDEYP 660


>AB013287-1|BAA87893.1|  190|Apis mellifera calmodulin kinase II
           protein.
          Length = 190

 Score =  108 bits (259), Expect = 8e-26
 Identities = 57/154 (37%), Positives = 91/154 (59%), Gaps = 10/154 (6%)

Query: 118 FSDSLTKFYASQVILALEYLHYCEVVHRDIKPENILIDSNGY---LKLCDFGFCKVLK-- 172
           +S++       Q++ ++ + H+  VVHRD+KPEN+L+ S      +KL DFG    ++  
Sbjct: 6   YSEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGE 65

Query: 173 KKTWT-LCGTPEYLAPEVITSKGYSFSVDWWALGDLSFEMEAGHPPFFASDPNKLYEKVL 231
            + W    GTP YL+PEV+  + Y   VD WA G + + +  G+PPF+  D ++LY ++ 
Sbjct: 66  AQAWFGFAGTPGYLSPEVLKKEPYGKPVDIWACGVILYILLVGYPPFWDEDQHRLYAQIK 125

Query: 232 VGSYKCP----ENMSPECKNLLKGLLQVDPSKRI 261
            GSY  P    + ++PE KNL+  +L V+PSKRI
Sbjct: 126 TGSYDYPSPEWDTVTPEAKNLINQMLTVNPSKRI 159


>AB013288-1|BAA87894.1|  149|Apis mellifera protein kinase C
           protein.
          Length = 149

 Score =  107 bits (258), Expect = 1e-25
 Identities = 52/148 (35%), Positives = 92/148 (62%), Gaps = 4/148 (2%)

Query: 39  LVRDKITFTYHAMKVVEKSVVVERNHAKHLILEKKILQC-IQFPFVVTLDVAFKDNLYLY 97
           L   K T   +A+K+++K ++++ +  +  ++EK++L    + PF+V L   F+    LY
Sbjct: 2   LAERKGTDELYAIKILKKDIIIQDDDVECTMVEKRVLALSTKPPFLVQLHSCFQTMDRLY 61

Query: 98  FILPYIAGGEMFTYIQKYGIFSDSLTKFYASQVILALEYLHYCEVVHRDIKPENILIDSN 157
           F++ Y+ GG++   IQ+ G F + +  FYAS++ + L +LH   +V+RD+K +N+L+D +
Sbjct: 62  FVMEYVNGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVLLDQD 121

Query: 158 GYLKLCDFGFCK---VLKKKTWTLCGTP 182
           G++K+ DFG CK      K T T CGTP
Sbjct: 122 GHIKIADFGMCKEGISGDKTTKTFCGTP 149


>DQ013068-1|AAY81956.1|  931|Apis mellifera dusty protein kinase
           isoform B protein.
          Length = 931

 Score = 71.7 bits (168), Expect = 8e-15
 Identities = 52/145 (35%), Positives = 74/145 (51%), Gaps = 12/145 (8%)

Query: 127 ASQVILALEYLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVLKKKTWTLCGTPEYLA 186
           A  V+  + YLH   +VHRD+K +N+L+D     KL DFGFC        ++ GTP ++A
Sbjct: 703 ALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCITEVMMLGSIVGTPVHMA 762

Query: 187 PEVITSKGYSFSVDWWALGDLSFEMEAGH---PPFFASDPNK-----LYEKVLVGSYKCP 238
           PE++ S  Y  SVD +A G L + + AGH   P  F    NK       +K L+     P
Sbjct: 763 PELL-SGHYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWTSVKKALMIVGIRP 821

Query: 239 ENM---SPECKNLLKGLLQVDPSKR 260
           E +     EC  L++     +PSKR
Sbjct: 822 ERLPSFDDECWRLMEQCWSGEPSKR 846


>DQ013067-1|AAY81955.1|  969|Apis mellifera dusty protein kinase
           isoform A protein.
          Length = 969

 Score = 71.7 bits (168), Expect = 8e-15
 Identities = 52/145 (35%), Positives = 74/145 (51%), Gaps = 12/145 (8%)

Query: 127 ASQVILALEYLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVLKKKTWTLCGTPEYLA 186
           A  V+  + YLH   +VHRD+K +N+L+D     KL DFGFC        ++ GTP ++A
Sbjct: 741 ALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCITEVMMLGSIVGTPVHMA 800

Query: 187 PEVITSKGYSFSVDWWALGDLSFEMEAGH---PPFFASDPNK-----LYEKVLVGSYKCP 238
           PE++ S  Y  SVD +A G L + + AGH   P  F    NK       +K L+     P
Sbjct: 801 PELL-SGHYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWTSVKKALMIVGIRP 859

Query: 239 ENM---SPECKNLLKGLLQVDPSKR 260
           E +     EC  L++     +PSKR
Sbjct: 860 ERLPSFDDECWRLMEQCWSGEPSKR 884


>AB183889-1|BAD86829.1|  316|Apis mellifera Mos protein.
          Length = 316

 Score = 64.1 bits (149), Expect = 2e-12
 Identities = 39/125 (31%), Positives = 61/125 (48%), Gaps = 2/125 (1%)

Query: 130 VILALEYLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVL--KKKTWTLCGTPEYLAP 187
           +  AL++ H   +VH D+KP+NIL+  NG  KL DFG   ++    +     GTP Y AP
Sbjct: 164 ITCALQFCHNAGIVHADVKPKNILMSKNGQPKLTDFGSSVLIGAPNEIDKFYGTPGYTAP 223

Query: 188 EVITSKGYSFSVDWWALGDLSFEMEAGHPPFFASDPNKLYEKVLVGSYKCPENMSPECKN 247
           EVI     + + D ++LG ++++M     PF     + +      G     +N+  E K 
Sbjct: 224 EVIKQNRPTPAADIYSLGIVAWQMLFRKLPFAGLHSHTIIYLSAKGHRPIDDNIDDEFKG 283

Query: 248 LLKGL 252
             K L
Sbjct: 284 TYKTL 288


>AY921579-1|AAX14899.1|  996|Apis mellifera ephrin receptor protein.
          Length = 996

 Score = 49.6 bits (113), Expect = 4e-08
 Identities = 42/185 (22%), Positives = 82/185 (44%), Gaps = 10/185 (5%)

Query: 69  ILEKKILQCIQFPFVVTLDVAFKDNLYLYFILPYIAGGEMFTYIQKY-GIFSDSLTKFYA 127
           + E  I+   + P V+ L      +  +  I  ++  G + T+++   G F         
Sbjct: 682 LTEASIMGQFEHPNVIFLQGVVTKSNPVMIITEFMENGSLDTFLRANDGKFQVLQLVGML 741

Query: 128 SQVILALEYLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVLKKKT---WTLCG---T 181
             +   ++YL     VHRD+   N+L+++    K+ DFG  + ++  T   +T  G    
Sbjct: 742 RGIASGMQYLAEMNYVHRDLAARNVLVNAALVCKIADFGLSREIESATEGAYTTRGGKIP 801

Query: 182 PEYLAPEVITSKGYSFSVDWWALGDLSFE-MEAGHPPFFASDPNKLYEKVLVGSYKCPEN 240
             + APE I  + ++ + D W++G + +E M  G  P++      + + +  G Y+ P  
Sbjct: 802 VRWTAPEAIAFRKFTSASDVWSMGIVCWEVMSYGERPYWNWSNQDVIKSIEKG-YRLPAP 860

Query: 241 MS-PE 244
           M  PE
Sbjct: 861 MDCPE 865


>AB072429-1|BAB83990.1|  388|Apis mellifera IP3phosphatase protein.
          Length = 388

 Score = 21.8 bits (44), Expect = 8.5
 Identities = 9/19 (47%), Positives = 11/19 (57%)

Query: 236 KCPENMSPECKNLLKGLLQ 254
           K P+   PECK   KG L+
Sbjct: 146 KFPQEFFPECKWSRKGFLR 164


  Database: bee
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 140,377
  Number of sequences in database:  429
  
Lambda     K      H
   0.322    0.141    0.439 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 103,475
Number of Sequences: 429
Number of extensions: 4473
Number of successful extensions: 16
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8
length of query: 330
length of database: 140,377
effective HSP length: 58
effective length of query: 272
effective length of database: 115,495
effective search space: 31414640
effective search space used: 31414640
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 44 (21.8 bits)

- SilkBase 1999-2023 -