BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000982-TA|BGIBMGA000982-PA|IPR002290|Serine/threonine protein kinase, IPR000961|Protein kinase, C-terminal, IPR008271|Serine/threonine protein kinase, active site, IPR000719|Protein kinase, IPR011009|Protein kinase-like (330 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 251 7e-69 AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 108 8e-26 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 107 1e-25 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 72 8e-15 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 72 8e-15 AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 64 2e-12 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 50 4e-08 AB072429-1|BAB83990.1| 388|Apis mellifera IP3phosphatase protein. 22 8.5 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 251 bits (614), Expect = 7e-69 Identities = 123/300 (41%), Positives = 184/300 (61%), Gaps = 5/300 (1%) Query: 17 DKTVDDFDIVKAIGNGAYGEVFLVRDK-ITFTYHAMKVVEKSVVVERNHAKHLILEKKIL 75 D + D + +G G +G V LV+ + A+K ++K+ +VE +H++ EK+I+ Sbjct: 361 DLRLQDLRPLATLGVGGFGRVELVQIAGDSSRSFALKQMKKAQIVETRQQQHIMSEKRIM 420 Query: 76 QCIQFPFVVTLDVAFKDNLYLYFILPYIAGGEMFTYIQKYGIFSDSLTKFYASQVILALE 135 FVV L FKD YLY ++ GGE++T ++ G F D T+FY + V+ A + Sbjct: 421 GEADCDFVVKLFKTFKDRKYLYMLMEACLGGELWTVLRDKGHFDDGTTRFYTACVVEAFD 480 Query: 136 YLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVLK--KKTWTLCGTPEYLAPEVITSK 193 YLH +++RD+KPEN+L+DS GY+KL DFGF K L +KTWT CGTPEY+APEVI +K Sbjct: 481 YLHSRNIIYRDLKPENLLLDSQGYVKLVDFGFAKRLDHGRKTWTFCGTPEYVAPEVILNK 540 Query: 194 GYSFSVDWWALGDLSFEMEAGHPPFFASDPNKLYEKVLVG--SYKCPENMSPECKNLLKG 251 G+ S D+W+LG L FE+ G PPF DP K Y +L G + + P +++ L+K Sbjct: 541 GHDISADYWSLGVLMFELLTGTPPFTGGDPMKTYNIILKGIDAIEFPRSITRNATALIKK 600 Query: 252 LLQVDPSKRIGSLKGGIYEIKSHTWFNGLDWQAIFHQKLIAPFVPICSSPGDTSNFPEIP 311 L + +P++R+G KGGI EI+ H WF+G +W+ + + L P +P + DT+NF E P Sbjct: 601 LCRDNPAERLGYQKGGISEIQKHKWFDGFNWEGLRARTLEPPIMPRVQNATDTTNFDEYP 660 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 108 bits (259), Expect = 8e-26 Identities = 57/154 (37%), Positives = 91/154 (59%), Gaps = 10/154 (6%) Query: 118 FSDSLTKFYASQVILALEYLHYCEVVHRDIKPENILIDSNGY---LKLCDFGFCKVLK-- 172 +S++ Q++ ++ + H+ VVHRD+KPEN+L+ S +KL DFG ++ Sbjct: 6 YSEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGE 65 Query: 173 KKTWT-LCGTPEYLAPEVITSKGYSFSVDWWALGDLSFEMEAGHPPFFASDPNKLYEKVL 231 + W GTP YL+PEV+ + Y VD WA G + + + G+PPF+ D ++LY ++ Sbjct: 66 AQAWFGFAGTPGYLSPEVLKKEPYGKPVDIWACGVILYILLVGYPPFWDEDQHRLYAQIK 125 Query: 232 VGSYKCP----ENMSPECKNLLKGLLQVDPSKRI 261 GSY P + ++PE KNL+ +L V+PSKRI Sbjct: 126 TGSYDYPSPEWDTVTPEAKNLINQMLTVNPSKRI 159 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 107 bits (258), Expect = 1e-25 Identities = 52/148 (35%), Positives = 92/148 (62%), Gaps = 4/148 (2%) Query: 39 LVRDKITFTYHAMKVVEKSVVVERNHAKHLILEKKILQC-IQFPFVVTLDVAFKDNLYLY 97 L K T +A+K+++K ++++ + + ++EK++L + PF+V L F+ LY Sbjct: 2 LAERKGTDELYAIKILKKDIIIQDDDVECTMVEKRVLALSTKPPFLVQLHSCFQTMDRLY 61 Query: 98 FILPYIAGGEMFTYIQKYGIFSDSLTKFYASQVILALEYLHYCEVVHRDIKPENILIDSN 157 F++ Y+ GG++ IQ+ G F + + FYAS++ + L +LH +V+RD+K +N+L+D + Sbjct: 62 FVMEYVNGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVLLDQD 121 Query: 158 GYLKLCDFGFCK---VLKKKTWTLCGTP 182 G++K+ DFG CK K T T CGTP Sbjct: 122 GHIKIADFGMCKEGISGDKTTKTFCGTP 149 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 71.7 bits (168), Expect = 8e-15 Identities = 52/145 (35%), Positives = 74/145 (51%), Gaps = 12/145 (8%) Query: 127 ASQVILALEYLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVLKKKTWTLCGTPEYLA 186 A V+ + YLH +VHRD+K +N+L+D KL DFGFC ++ GTP ++A Sbjct: 703 ALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCITEVMMLGSIVGTPVHMA 762 Query: 187 PEVITSKGYSFSVDWWALGDLSFEMEAGH---PPFFASDPNK-----LYEKVLVGSYKCP 238 PE++ S Y SVD +A G L + + AGH P F NK +K L+ P Sbjct: 763 PELL-SGHYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWTSVKKALMIVGIRP 821 Query: 239 ENM---SPECKNLLKGLLQVDPSKR 260 E + EC L++ +PSKR Sbjct: 822 ERLPSFDDECWRLMEQCWSGEPSKR 846 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 71.7 bits (168), Expect = 8e-15 Identities = 52/145 (35%), Positives = 74/145 (51%), Gaps = 12/145 (8%) Query: 127 ASQVILALEYLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVLKKKTWTLCGTPEYLA 186 A V+ + YLH +VHRD+K +N+L+D KL DFGFC ++ GTP ++A Sbjct: 741 ALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCITEVMMLGSIVGTPVHMA 800 Query: 187 PEVITSKGYSFSVDWWALGDLSFEMEAGH---PPFFASDPNK-----LYEKVLVGSYKCP 238 PE++ S Y SVD +A G L + + AGH P F NK +K L+ P Sbjct: 801 PELL-SGHYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWTSVKKALMIVGIRP 859 Query: 239 ENM---SPECKNLLKGLLQVDPSKR 260 E + EC L++ +PSKR Sbjct: 860 ERLPSFDDECWRLMEQCWSGEPSKR 884 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 64.1 bits (149), Expect = 2e-12 Identities = 39/125 (31%), Positives = 61/125 (48%), Gaps = 2/125 (1%) Query: 130 VILALEYLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVL--KKKTWTLCGTPEYLAP 187 + AL++ H +VH D+KP+NIL+ NG KL DFG ++ + GTP Y AP Sbjct: 164 ITCALQFCHNAGIVHADVKPKNILMSKNGQPKLTDFGSSVLIGAPNEIDKFYGTPGYTAP 223 Query: 188 EVITSKGYSFSVDWWALGDLSFEMEAGHPPFFASDPNKLYEKVLVGSYKCPENMSPECKN 247 EVI + + D ++LG ++++M PF + + G +N+ E K Sbjct: 224 EVIKQNRPTPAADIYSLGIVAWQMLFRKLPFAGLHSHTIIYLSAKGHRPIDDNIDDEFKG 283 Query: 248 LLKGL 252 K L Sbjct: 284 TYKTL 288 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 49.6 bits (113), Expect = 4e-08 Identities = 42/185 (22%), Positives = 82/185 (44%), Gaps = 10/185 (5%) Query: 69 ILEKKILQCIQFPFVVTLDVAFKDNLYLYFILPYIAGGEMFTYIQKY-GIFSDSLTKFYA 127 + E I+ + P V+ L + + I ++ G + T+++ G F Sbjct: 682 LTEASIMGQFEHPNVIFLQGVVTKSNPVMIITEFMENGSLDTFLRANDGKFQVLQLVGML 741 Query: 128 SQVILALEYLHYCEVVHRDIKPENILIDSNGYLKLCDFGFCKVLKKKT---WTLCG---T 181 + ++YL VHRD+ N+L+++ K+ DFG + ++ T +T G Sbjct: 742 RGIASGMQYLAEMNYVHRDLAARNVLVNAALVCKIADFGLSREIESATEGAYTTRGGKIP 801 Query: 182 PEYLAPEVITSKGYSFSVDWWALGDLSFE-MEAGHPPFFASDPNKLYEKVLVGSYKCPEN 240 + APE I + ++ + D W++G + +E M G P++ + + + G Y+ P Sbjct: 802 VRWTAPEAIAFRKFTSASDVWSMGIVCWEVMSYGERPYWNWSNQDVIKSIEKG-YRLPAP 860 Query: 241 MS-PE 244 M PE Sbjct: 861 MDCPE 865 >AB072429-1|BAB83990.1| 388|Apis mellifera IP3phosphatase protein. Length = 388 Score = 21.8 bits (44), Expect = 8.5 Identities = 9/19 (47%), Positives = 11/19 (57%) Query: 236 KCPENMSPECKNLLKGLLQ 254 K P+ PECK KG L+ Sbjct: 146 KFPQEFFPECKWSRKGFLR 164 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.322 0.141 0.439 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 103,475 Number of Sequences: 429 Number of extensions: 4473 Number of successful extensions: 16 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 8 length of query: 330 length of database: 140,377 effective HSP length: 58 effective length of query: 272 effective length of database: 115,495 effective search space: 31414640 effective search space used: 31414640 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 44 (21.8 bits)
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