Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000980-TA|BGIBMGA000980-PA|IPR007303|TIP41-like protein
         (131 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g34270.1 68417.m04872 TIP41-like family protein contains Pfam...    70   6e-13
At3g57790.1 68416.m06438 glycoside hydrolase family 28 protein /...    30   0.58 
At5g06720.1 68418.m00760 peroxidase, putative identical to perox...    28   1.8  
At4g16120.1 68417.m02443 phytochelatin synthetase-related contai...    28   2.3  
At5g06730.1 68418.m00761 peroxidase, putative similar to peroxid...    27   3.1  
At3g17520.1 68416.m02238 late embryogenesis abundant domain-cont...    27   3.1  
At3g20490.1 68416.m02595 expressed protein                             26   7.1  
At1g63810.1 68414.m07221 nucleolar RNA-associated family protein...    26   7.1  
At5g33410.1 68418.m03993 replication protein-related weak simila...    26   9.4  
At5g13070.1 68418.m01498 MSF1-like family protein similar to px1...    26   9.4  
At3g09970.1 68416.m01196 calcineurin-like phosphoesterase family...    26   9.4  
At1g72810.1 68414.m08417 threonine synthase, putative strong sim...    26   9.4  
At1g56510.1 68414.m06498 disease resistance protein (TIR-NBS-LRR...    26   9.4  

>At4g34270.1 68417.m04872 TIP41-like family protein contains Pfam
           PF04176: TIP41-like family; identical to cDNA putative
           cytoskeletal protein mRNA, partial cds GI:5031529
          Length = 290

 Score = 69.7 bits (163), Expect = 6e-13
 Identities = 53/142 (37%), Positives = 70/142 (49%), Gaps = 19/142 (13%)

Query: 1   MVFPKNRLTLAHK-SGASINFNPLDALKRVAST-VEPVEVSCSEVWMQARPYAEKLKKSF 58
           MVF +N L L H  S   I+FN  DAL       + PVEV  +  W      ++++   +
Sbjct: 60  MVFGENALVLKHLGSNTKIHFNAFDALAGWKQEGLPPVEVPAAAQWKFRSKPSQQVILDY 119

Query: 59  DWTFCTDYKGSI---SDNITV-------------WET-EESIDFELLKQKNQILFYHDLT 101
           D+TF T Y GS     D  TV             WE  E+ ID   L  K  ILFY ++ 
Sbjct: 120 DYTFTTPYCGSEVVEKDKETVEAKANPKGEATLQWENCEDQIDLAALSLKEPILFYDEVV 179

Query: 102 LFEDELHDHGVSKLSVKIVSTP 123
           L+EDEL D+GVS L+VK+   P
Sbjct: 180 LYEDELADNGVSLLTVKVRVMP 201


>At3g57790.1 68416.m06438 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein weak
           similarity to SP|P15922
           Exo-poly-alpha-D-galacturonosidase precursor (EC
           3.2.1.82) (Exo-PG) {Erwinia chrysanthemi}; contains
           PF00295: Glycosyl hydrolases family 28
          Length = 490

 Score = 29.9 bits (64), Expect = 0.58
 Identities = 14/50 (28%), Positives = 29/50 (58%), Gaps = 4/50 (8%)

Query: 38  VSCSEVWMQARPYAEKLKKSFDWTFCTDYKGSISDNITVWETEESIDFEL 87
           ++ ++ W++ +  A KL  S  W    D+KG + DNIT++E+   +  ++
Sbjct: 260 LTATDCWIRTKSSAIKLG-SASWF---DFKGLVFDNITIFESHRGLGMQI 305


>At5g06720.1 68418.m00760 peroxidase, putative identical to
           peroxidase [Arabidopsis thaliana]
           gi|1491617|emb|CAA68212
          Length = 335

 Score = 28.3 bits (60), Expect = 1.8
 Identities = 16/55 (29%), Positives = 24/55 (43%)

Query: 20  FNPLDALKRVASTVEPVEVSCSEVWMQARPYAEKLKKSFDWTFCTDYKGSISDNI 74
           FN +D +K       P  VSCS+V   A   +  L     WT     + S++ N+
Sbjct: 107 FNVVDNIKTALENACPGVVSCSDVLALASEASVSLAGGPSWTVLLGRRDSLTANL 161


>At4g16120.1 68417.m02443 phytochelatin synthetase-related contains
           Pfam PF04833: Phytochelatin synthetase-like conserved
           region; supporting cDNA gi|26449620|dbj|AK117261.1|
          Length = 661

 Score = 27.9 bits (59), Expect = 2.3
 Identities = 14/47 (29%), Positives = 23/47 (48%), Gaps = 3/47 (6%)

Query: 49  PYAEKLKKSFDWTFCTDYKGSISDNITV--WETEESID-FELLKQKN 92
           P  +    S +W   TDY+G  +  +TV  W   + +D F  ++ KN
Sbjct: 493 PCGDNCGVSINWHLATDYRGGWTARVTVFNWGETDFVDWFTAVQMKN 539


>At5g06730.1 68418.m00761 peroxidase, putative similar to peroxidase
           [Arabidopsis thaliana] gi|1491617|emb|CAA68212
          Length = 358

 Score = 27.5 bits (58), Expect = 3.1
 Identities = 14/56 (25%), Positives = 25/56 (44%)

Query: 20  FNPLDALKRVASTVEPVEVSCSEVWMQARPYAEKLKKSFDWTFCTDYKGSISDNIT 75
           FN +D++K       P  VSCS++   A   +  L     WT     +  ++ N++
Sbjct: 108 FNVVDSIKTALENACPGIVSCSDILALASEASVSLAGGPSWTVLLGRRDGLTANLS 163


>At3g17520.1 68416.m02238 late embryogenesis abundant
           domain-containing protein / LEA domain-containing
           protein low similarity to PIR|S04045|S04045 embryonic
           abundant protein D-29 [Gossypium hirsutum]; contains
           Pfam profile PF02987: Late embryogenesis abundant
           protein
          Length = 298

 Score = 27.5 bits (58), Expect = 3.1
 Identities = 13/33 (39%), Positives = 21/33 (63%)

Query: 24  DALKRVASTVEPVEVSCSEVWMQARPYAEKLKK 56
           DA +++A TVE  +   S++   A+  AEKLK+
Sbjct: 195 DAKEKLAETVETAKEKASDMTSAAKEKAEKLKE 227


>At3g20490.1 68416.m02595 expressed protein
          Length = 458

 Score = 26.2 bits (55), Expect = 7.1
 Identities = 18/77 (23%), Positives = 31/77 (40%), Gaps = 3/77 (3%)

Query: 34  EPVEVSC-SEVWMQARPYAEKLKK-SFDWTFCTDYKGSISDNITVWETEESIDFELLKQK 91
           EP  + C  ++W    P + K++  +FD   C DY  SI    T  +   ++        
Sbjct: 216 EPGSIPCIKDLWKDFSPASSKIQTPAFD-DVCQDYFISIKTTSTAQKQSSAVASSSNSGN 274

Query: 92  NQILFYHDLTLFEDELH 108
           + +  +    LF D  H
Sbjct: 275 HNLTGFQQTELFHDFSH 291


>At1g63810.1 68414.m07221 nucleolar RNA-associated family protein /
           Nrap family protein contains Pfam profile PF03813: Nrap
           protein; similar to nucleolar RNA-associated protein
           alpha (GI:18539461) [Mus musculus]
          Length = 1053

 Score = 26.2 bits (55), Expect = 7.1
 Identities = 13/40 (32%), Positives = 24/40 (60%), Gaps = 1/40 (2%)

Query: 68  GSISDNITVWETEESIDFELLKQKNQILFYHDLTLFEDEL 107
           G IS++ TVWET++     ++KQ  + +    L+L  D++
Sbjct: 487 GRISES-TVWETQQWTKHLIMKQIVEYILKRHLSLTSDDI 525


>At5g33410.1 68418.m03993 replication protein-related weak
           similarity to Replication Protein A 70  kDa DNA-binding
           subunit (RP-A) (RF-A) (Replication factor-A protein 1)
           (Single-stranded DNA-binding protein) (DMRPA1)
           (GI:2498844)(SP:Q24492) [Drosophila melanogaster]
          Length = 251

 Score = 25.8 bits (54), Expect = 9.4
 Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 7/53 (13%)

Query: 28  RVASTVEPVEVSCSEVWMQAR------PYAEKLKKSF-DWTFCTDYKGSISDN 73
           RV S V  ++ +CS + ++ +      P  E  K  F DW F +D + S +DN
Sbjct: 79  RVISLVHTLKYTCSPLHIKFKWDTFVSPIPEVEKSDFSDWIFPSDLENSSTDN 131


>At5g13070.1 68418.m01498 MSF1-like family protein similar to px19
           [Gallus gallus] GI:969170; contains Pfam profile
           PF04707: MSF1-like conserved region
          Length = 183

 Score = 25.8 bits (54), Expect = 9.4
 Identities = 15/30 (50%), Positives = 17/30 (56%), Gaps = 3/30 (10%)

Query: 17  SINFNPLDALKRVASTVEPVEVSCSEVWMQ 46
           SI   PL AL   AS  E VE  C+E +MQ
Sbjct: 134 SIRIKPLSAL---ASMAEKVEQKCAEKFMQ 160


>At3g09970.1 68416.m01196 calcineurin-like phosphoesterase family
           protein contains Pfam profile: PF00149 Calcineurin-like
           phosphoesterase
          Length = 309

 Score = 25.8 bits (54), Expect = 9.4
 Identities = 13/38 (34%), Positives = 23/38 (60%)

Query: 42  EVWMQARPYAEKLKKSFDWTFCTDYKGSISDNITVWET 79
           ++ +Q R +A K+K +F+      YKGSI D  + +E+
Sbjct: 125 DMHLQGRRWAGKIKATFNSVKGMAYKGSIYDAGSTFES 162


>At1g72810.1 68414.m08417 threonine synthase, putative strong
           similarity to SP|Q9S7B5 Threonine synthase, chloroplast
           precursor (EC 4.2.3.1) (TS) {Arabidopsis thaliana}
          Length = 516

 Score = 25.8 bits (54), Expect = 9.4
 Identities = 13/29 (44%), Positives = 17/29 (58%)

Query: 6   NRLTLAHKSGASINFNPLDALKRVASTVE 34
           N L L +KSG   +FNPL A    AS ++
Sbjct: 362 NPLYLHYKSGFKEDFNPLKANTTFASAIQ 390


>At1g56510.1 68414.m06498 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1007

 Score = 25.8 bits (54), Expect = 9.4
 Identities = 12/33 (36%), Positives = 17/33 (51%)

Query: 78  ETEESIDFELLKQKNQILFYHDLTLFEDELHDH 110
           E+   + FE L +K+Q LF +    F  E  DH
Sbjct: 415 ESVLKVGFESLNEKDQALFLYITVFFNYECADH 447


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.319    0.133    0.400 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,117,370
Number of Sequences: 28952
Number of extensions: 114447
Number of successful extensions: 264
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 255
Number of HSP's gapped (non-prelim): 14
length of query: 131
length of database: 12,070,560
effective HSP length: 74
effective length of query: 57
effective length of database: 9,928,112
effective search space: 565902384
effective search space used: 565902384
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 54 (25.8 bits)