Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000972-TA|BGIBMGA000972-PA|IPR001452|Src homology-3
         (72 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g39880.1 68417.m05650 ribosomal protein L23 family protein co...    29   0.28 
At3g17470.1 68416.m02232 RelA/SpoT domain-containing protein / c...    28   0.66 
At4g12240.1 68417.m01941 zinc finger (C2H2 type) family protein ...    27   1.1  
At4g26900.1 68417.m03870 imidazole glycerol phosphate synthase h...    27   1.5  
At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3)...    27   2.0  
At3g49520.1 68416.m05412 F-box family protein contains weak hit ...    27   2.0  
At5g10840.1 68418.m01259 endomembrane protein 70, putative TM4 f...    26   3.5  
At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4 f...    25   4.6  
At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family pro...    25   4.6  
At2g05600.1 68415.m00596 hypothetical protein                          25   4.6  
At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family...    25   8.1  

>At4g39880.1 68417.m05650 ribosomal protein L23 family protein
           contains Pfam profile  PF00276: ribosomal protein L23
          Length = 178

 Score = 29.5 bits (63), Expect = 0.28
 Identities = 15/43 (34%), Positives = 22/43 (51%), Gaps = 1/43 (2%)

Query: 28  LYPFKAIESGDLSLEKGAEY-EVIDDSQDHWWKVKDENGYGDF 69
           L+P K IE    S  KG+ + E  DD + HW   K++   G +
Sbjct: 98  LFPVKYIEEDRKSKVKGSSFVEEEDDKKSHWLDRKEKREIGGY 140


>At3g17470.1 68416.m02232 RelA/SpoT domain-containing protein /
           calcium-binding EF-hand family protein contains
           INTERPRO:IPR002048 calcium-binding EF-hand domain, Pfam
           profile PF04607: Region found in RelA / SpoT proteins
          Length = 583

 Score = 28.3 bits (60), Expect = 0.66
 Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 1/53 (1%)

Query: 16  PKEKPRTK-IVVALYPFKAIESGDLSLEKGAEYEVIDDSQDHWWKVKDENGYG 67
           PKE  R K I++A     AI   D+S  K   ++   + +D  + + D+NG G
Sbjct: 436 PKEAKRLKAIMLAAADLAAIRLKDISSNKHQSFKTTTNQRDRVFCLLDKNGDG 488


>At4g12240.1 68417.m01941 zinc finger (C2H2 type) family protein
           contains Pfam domain, PF00096: Zinc finger, C2H2 type
          Length = 364

 Score = 27.5 bits (58), Expect = 1.1
 Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 3/32 (9%)

Query: 38  DLSLEKGAEYEVID--DSQDHWWKVKDENGYG 67
           D  +E G E E ++  D  D WWK+  E+G G
Sbjct: 328 DDEIENGTEVEPVEIGDGVD-WWKIDTEDGVG 358


>At4g26900.1 68417.m03870 imidazole glycerol phosphate synthase
           hisHF, chloroplast / IGP synthase / ImGPP synthase /
           IGPS identical to SP|Q9SZ30 Imidazole glycerol phosphate
           synthase hisHF, chloroplast precursor (IGP synthase)
           {Arabidopsis thaliana}
          Length = 592

 Score = 27.1 bits (57), Expect = 1.5
 Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 3/45 (6%)

Query: 15  KPKEKPRTKI---VVALYPFKAIESGDLSLEKGAEYEVIDDSQDH 56
           KP E   +K+   V+A    +  + GDL + KG +Y+V + S ++
Sbjct: 271 KPMEGKASKLAKRVIACLDVRTNDKGDLVVTKGDQYDVREQSNEN 315


>At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3)
           nearly identical to SH3 domain-containing protein 3
           [Arabidopsis thaliana] GI:16974680; contains Pfam
           profile PF00018: SH3 domain
          Length = 351

 Score = 26.6 bits (56), Expect = 2.0
 Identities = 14/46 (30%), Positives = 20/46 (43%)

Query: 12  PSAKPKEKPRTKIVVALYPFKAIESGDLSLEKGAEYEVIDDSQDHW 57
           P+    EK    +   ++PF A    +L L+KG    V   SQ  W
Sbjct: 273 PTENGSEKTSYFLAEVIHPFSAASEKELDLDKGDYIVVRKVSQTGW 318


>At3g49520.1 68416.m05412 F-box family protein contains weak hit to
           Pfam:PF00646 F-box domain and weak hit to TIGRFAM
           TIGR01640 : F-box protein interaction domain
          Length = 388

 Score = 26.6 bits (56), Expect = 2.0
 Identities = 13/32 (40%), Positives = 17/32 (53%)

Query: 39  LSLEKGAEYEVIDDSQDHWWKVKDENGYGDFL 70
           L+ E  +  +V D S D W  V D+N Y D L
Sbjct: 159 LNEENESHIDVYDFSSDSWRTVPDDNPYRDEL 190


>At5g10840.1 68418.m01259 endomembrane protein 70, putative TM4
           family;
          Length = 648

 Score = 25.8 bits (54), Expect = 3.5
 Identities = 13/48 (27%), Positives = 23/48 (47%)

Query: 13  SAKPKEKPRTKIVVALYPFKAIESGDLSLEKGAEYEVIDDSQDHWWKV 60
           SA P+E  + K ++  Y     ES      +   Y ++ D+Q HW+ +
Sbjct: 242 SATPQEVEQKKEIIFTYDVDFQESEVKWASRWDTYLLMSDNQIHWFSI 289


>At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4
           family;
          Length = 644

 Score = 25.4 bits (53), Expect = 4.6
 Identities = 13/48 (27%), Positives = 22/48 (45%)

Query: 13  SAKPKEKPRTKIVVALYPFKAIESGDLSLEKGAEYEVIDDSQDHWWKV 60
           SA P+E    K ++  Y     ES      +   Y ++ D+Q HW+ +
Sbjct: 238 SATPQEVENKKEIIFTYDVDFQESEVKWASRWDAYLLMSDNQIHWFSI 285


>At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family
           protein low similarity to ubiquitin-conjugating
           BIR-domain enzyme APOLLON [Homo sapiens] GI:8489831,
           ubiquitin-conjugating enzyme [Mus musculus] GI:3319990;
           contains Pfam profile PF00179: Ubiquitin-conjugating
           enzyme
          Length = 907

 Score = 25.4 bits (53), Expect = 4.6
 Identities = 10/24 (41%), Positives = 15/24 (62%)

Query: 32  KAIESGDLSLEKGAEYEVIDDSQD 55
           K +E   ++L+ G  YEV+ D QD
Sbjct: 123 KIVERVSVTLDDGTNYEVLVDGQD 146


>At2g05600.1 68415.m00596 hypothetical protein
          Length = 239

 Score = 25.4 bits (53), Expect = 4.6
 Identities = 8/21 (38%), Positives = 13/21 (61%)

Query: 51  DDSQDHWWKVKDENGYGDFLF 71
           D  Q+ WW+V+D N   + +F
Sbjct: 130 DCKQEFWWRVEDNNNTWEKIF 150


>At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family
           protein contains Pfam profile: PF00149 calcineurin-like
           phosphoesterase
          Length = 1012

 Score = 24.6 bits (51), Expect = 8.1
 Identities = 11/33 (33%), Positives = 17/33 (51%)

Query: 35  ESGDLSLEKGAEYEVIDDSQDHWWKVKDENGYG 67
           +SGD S  K   Y+ + + QD W+    + G G
Sbjct: 347 KSGDASGRKELLYDHLAEKQDFWFDFMADTGDG 379


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.314    0.136    0.415 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,751,987
Number of Sequences: 28952
Number of extensions: 59632
Number of successful extensions: 93
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 85
Number of HSP's gapped (non-prelim): 11
length of query: 72
length of database: 12,070,560
effective HSP length: 52
effective length of query: 20
effective length of database: 10,565,056
effective search space: 211301120
effective search space used: 211301120
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 51 (24.6 bits)