BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000972-TA|BGIBMGA000972-PA|IPR001452|Src homology-3
(72 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g39880.1 68417.m05650 ribosomal protein L23 family protein co... 29 0.28
At3g17470.1 68416.m02232 RelA/SpoT domain-containing protein / c... 28 0.66
At4g12240.1 68417.m01941 zinc finger (C2H2 type) family protein ... 27 1.1
At4g26900.1 68417.m03870 imidazole glycerol phosphate synthase h... 27 1.5
At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3)... 27 2.0
At3g49520.1 68416.m05412 F-box family protein contains weak hit ... 27 2.0
At5g10840.1 68418.m01259 endomembrane protein 70, putative TM4 f... 26 3.5
At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4 f... 25 4.6
At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family pro... 25 4.6
At2g05600.1 68415.m00596 hypothetical protein 25 4.6
At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family... 25 8.1
>At4g39880.1 68417.m05650 ribosomal protein L23 family protein
contains Pfam profile PF00276: ribosomal protein L23
Length = 178
Score = 29.5 bits (63), Expect = 0.28
Identities = 15/43 (34%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Query: 28 LYPFKAIESGDLSLEKGAEY-EVIDDSQDHWWKVKDENGYGDF 69
L+P K IE S KG+ + E DD + HW K++ G +
Sbjct: 98 LFPVKYIEEDRKSKVKGSSFVEEEDDKKSHWLDRKEKREIGGY 140
>At3g17470.1 68416.m02232 RelA/SpoT domain-containing protein /
calcium-binding EF-hand family protein contains
INTERPRO:IPR002048 calcium-binding EF-hand domain, Pfam
profile PF04607: Region found in RelA / SpoT proteins
Length = 583
Score = 28.3 bits (60), Expect = 0.66
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Query: 16 PKEKPRTK-IVVALYPFKAIESGDLSLEKGAEYEVIDDSQDHWWKVKDENGYG 67
PKE R K I++A AI D+S K ++ + +D + + D+NG G
Sbjct: 436 PKEAKRLKAIMLAAADLAAIRLKDISSNKHQSFKTTTNQRDRVFCLLDKNGDG 488
>At4g12240.1 68417.m01941 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 364
Score = 27.5 bits (58), Expect = 1.1
Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 3/32 (9%)
Query: 38 DLSLEKGAEYEVID--DSQDHWWKVKDENGYG 67
D +E G E E ++ D D WWK+ E+G G
Sbjct: 328 DDEIENGTEVEPVEIGDGVD-WWKIDTEDGVG 358
>At4g26900.1 68417.m03870 imidazole glycerol phosphate synthase
hisHF, chloroplast / IGP synthase / ImGPP synthase /
IGPS identical to SP|Q9SZ30 Imidazole glycerol phosphate
synthase hisHF, chloroplast precursor (IGP synthase)
{Arabidopsis thaliana}
Length = 592
Score = 27.1 bits (57), Expect = 1.5
Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Query: 15 KPKEKPRTKI---VVALYPFKAIESGDLSLEKGAEYEVIDDSQDH 56
KP E +K+ V+A + + GDL + KG +Y+V + S ++
Sbjct: 271 KPMEGKASKLAKRVIACLDVRTNDKGDLVVTKGDQYDVREQSNEN 315
>At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3)
nearly identical to SH3 domain-containing protein 3
[Arabidopsis thaliana] GI:16974680; contains Pfam
profile PF00018: SH3 domain
Length = 351
Score = 26.6 bits (56), Expect = 2.0
Identities = 14/46 (30%), Positives = 20/46 (43%)
Query: 12 PSAKPKEKPRTKIVVALYPFKAIESGDLSLEKGAEYEVIDDSQDHW 57
P+ EK + ++PF A +L L+KG V SQ W
Sbjct: 273 PTENGSEKTSYFLAEVIHPFSAASEKELDLDKGDYIVVRKVSQTGW 318
>At3g49520.1 68416.m05412 F-box family protein contains weak hit to
Pfam:PF00646 F-box domain and weak hit to TIGRFAM
TIGR01640 : F-box protein interaction domain
Length = 388
Score = 26.6 bits (56), Expect = 2.0
Identities = 13/32 (40%), Positives = 17/32 (53%)
Query: 39 LSLEKGAEYEVIDDSQDHWWKVKDENGYGDFL 70
L+ E + +V D S D W V D+N Y D L
Sbjct: 159 LNEENESHIDVYDFSSDSWRTVPDDNPYRDEL 190
>At5g10840.1 68418.m01259 endomembrane protein 70, putative TM4
family;
Length = 648
Score = 25.8 bits (54), Expect = 3.5
Identities = 13/48 (27%), Positives = 23/48 (47%)
Query: 13 SAKPKEKPRTKIVVALYPFKAIESGDLSLEKGAEYEVIDDSQDHWWKV 60
SA P+E + K ++ Y ES + Y ++ D+Q HW+ +
Sbjct: 242 SATPQEVEQKKEIIFTYDVDFQESEVKWASRWDTYLLMSDNQIHWFSI 289
>At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4
family;
Length = 644
Score = 25.4 bits (53), Expect = 4.6
Identities = 13/48 (27%), Positives = 22/48 (45%)
Query: 13 SAKPKEKPRTKIVVALYPFKAIESGDLSLEKGAEYEVIDDSQDHWWKV 60
SA P+E K ++ Y ES + Y ++ D+Q HW+ +
Sbjct: 238 SATPQEVENKKEIIFTYDVDFQESEVKWASRWDAYLLMSDNQIHWFSI 285
>At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family
protein low similarity to ubiquitin-conjugating
BIR-domain enzyme APOLLON [Homo sapiens] GI:8489831,
ubiquitin-conjugating enzyme [Mus musculus] GI:3319990;
contains Pfam profile PF00179: Ubiquitin-conjugating
enzyme
Length = 907
Score = 25.4 bits (53), Expect = 4.6
Identities = 10/24 (41%), Positives = 15/24 (62%)
Query: 32 KAIESGDLSLEKGAEYEVIDDSQD 55
K +E ++L+ G YEV+ D QD
Sbjct: 123 KIVERVSVTLDDGTNYEVLVDGQD 146
>At2g05600.1 68415.m00596 hypothetical protein
Length = 239
Score = 25.4 bits (53), Expect = 4.6
Identities = 8/21 (38%), Positives = 13/21 (61%)
Query: 51 DDSQDHWWKVKDENGYGDFLF 71
D Q+ WW+V+D N + +F
Sbjct: 130 DCKQEFWWRVEDNNNTWEKIF 150
>At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 1012
Score = 24.6 bits (51), Expect = 8.1
Identities = 11/33 (33%), Positives = 17/33 (51%)
Query: 35 ESGDLSLEKGAEYEVIDDSQDHWWKVKDENGYG 67
+SGD S K Y+ + + QD W+ + G G
Sbjct: 347 KSGDASGRKELLYDHLAEKQDFWFDFMADTGDG 379
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.314 0.136 0.415
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,751,987
Number of Sequences: 28952
Number of extensions: 59632
Number of successful extensions: 93
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 85
Number of HSP's gapped (non-prelim): 11
length of query: 72
length of database: 12,070,560
effective HSP length: 52
effective length of query: 20
effective length of database: 10,565,056
effective search space: 211301120
effective search space used: 211301120
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 51 (24.6 bits)
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