BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000965-TA|BGIBMGA000965-PA|IPR013017|NHL, IPR000315|Zinc finger, B-box, IPR001841|Zinc finger, RING-type, IPR003649|B-box, C-terminal, IPR001258|NHL repeat (857 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 36 0.031 SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizo... 35 0.042 SPAPB17E12.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 33 0.17 SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 33 0.17 SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 33 0.17 SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 33 0.22 SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 31 0.51 SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 31 0.89 SPAC30C2.06c |dml1||mitochondrial genome maintenance protein |Sc... 30 1.2 SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1||... 30 1.6 SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 29 2.1 SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch... 29 3.6 SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 4.8 SPBP35G2.08c |air1||zinc knuckle TRAMP complex subunit Air1|Schi... 28 6.3 SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 28 6.3 SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe... 28 6.3 SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 27 8.3 >SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 897 Score = 35.5 bits (78), Expect = 0.031 Identities = 13/51 (25%), Positives = 27/51 (52%), Gaps = 1/51 (1%) Query: 33 DCKICDNKLCSPR-VLSCLHVFCEACIDKLMVNEAGDSLKFDLAVECPICK 82 +C +C + +P ++ C H C+ C+ L+ + G S + +CP+C+ Sbjct: 605 NCSVCLDPCLAPVFIIPCGHFTCQECMSMLVGQKYGSSSTSTIIAKCPMCR 655 >SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizosaccharomyces pombe|chr 3|||Manual Length = 963 Score = 35.1 bits (77), Expect = 0.042 Identities = 18/48 (37%), Positives = 21/48 (43%), Gaps = 5/48 (10%) Query: 34 CKICDNKLCSPRVLSCLHVFCEACIDKLMVNEAGDSLKFDLAVECPIC 81 CKICD C H FC C+ + +N AGD V CP C Sbjct: 711 CKICDEVAQDAIESRCHHTFCRLCVTE-YINAAGDGEN----VNCPSC 753 >SPAPB17E12.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 311 Score = 33.1 bits (72), Expect = 0.17 Identities = 14/25 (56%), Positives = 16/25 (64%) Query: 34 CKICDNKLCSPRVLSCLHVFCEACI 58 CKIC K+ +P VLS VFC CI Sbjct: 257 CKICGEKIKNPAVLSTGFVFCYPCI 281 >SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 456 Score = 33.1 bits (72), Expect = 0.17 Identities = 24/73 (32%), Positives = 31/73 (42%), Gaps = 12/73 (16%) Query: 33 DCKICDNKLCSPRVLSCLHVFCEACIDKLMVNEAGDSLKFDLAVECPICKQETKMLGGGA 92 +C IC N P L C HVFC C+ L K D CP+C+ + M A Sbjct: 359 ECAICSNVAYKPVRLGCSHVFCLHCLIIL------QKQKVDF---CPLCRAKEVM---KA 406 Query: 93 ASLPSDYVLTNIL 105 S D+ L N + Sbjct: 407 DSRNIDHALMNFM 419 >SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 486 Score = 33.1 bits (72), Expect = 0.17 Identities = 13/33 (39%), Positives = 17/33 (51%) Query: 26 DNGKHEQDCKICDNKLCSPRVLSCLHVFCEACI 58 +N E +C+IC L P V C H FC C+ Sbjct: 161 ENVSRELECQICFGMLYDPVVSPCGHTFCGPCL 193 >SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pombe|chr 2|||Manual Length = 387 Score = 32.7 bits (71), Expect = 0.22 Identities = 24/80 (30%), Positives = 39/80 (48%), Gaps = 8/80 (10%) Query: 93 ASLPSDYVLTNILDVSTMDQSVVCTCCKSKEPAVARCTDCSHFLCSNCNSAHEFMRCFEN 152 A+ PSD+ T I + +D S+ C C A T CSH CS C +++R Sbjct: 6 ATDPSDWNQTKIPSLKGLDSSLRCLICHEYFRAPL-ITSCSHTFCSFC--IRDYLR---E 59 Query: 153 HRVVPFDALRSSKEKTAVHK 172 H + P A R+ ++++ + K Sbjct: 60 HPMCP--ACRAPEQESRLRK 77 Score = 31.1 bits (67), Expect = 0.68 Identities = 11/25 (44%), Positives = 13/25 (52%) Query: 34 CKICDNKLCSPRVLSCLHVFCEACI 58 C IC +P + SC H FC CI Sbjct: 29 CLICHEYFRAPLITSCSHTFCSFCI 53 >SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 31.5 bits (68), Expect = 0.51 Identities = 24/63 (38%), Positives = 33/63 (52%), Gaps = 14/63 (22%) Query: 31 EQDCKIC--DNKLCSP--RVLSCLHVFCEACIDKLMVNEAGDSLKFDLAVECPICKQE-T 85 +++C IC + SP R L C H+F ACID ++ + DL CP+CKQ T Sbjct: 495 QRECTICLCEYSEESPLYRELPCHHIFHPACIDPYLLKNS------DL---CPLCKQSVT 545 Query: 86 KML 88 ML Sbjct: 546 NML 548 >SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 30.7 bits (66), Expect = 0.89 Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 4/51 (7%) Query: 30 HEQDCKICDNKLCSPRV-LSCLHVFCEACIDKLMVNEAGDSLKFDLAVECP 79 HE C+IC ++ C P C H FC AC + + + + + ++CP Sbjct: 127 HEGTCEICYDEGCLPFFSAECDHEFCLACYRQYLDSRISEG---ESVIQCP 174 >SPAC30C2.06c |dml1||mitochondrial genome maintenance protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 465 Score = 30.3 bits (65), Expect = 1.2 Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 2/66 (3%) Query: 176 CTRHAGESLKFYCCECEVGACTECLTVDHKVGEHRCERIVDAEQNIRAELRNFIVEANTR 235 CT G+ L + ++ E T+ +V C R+ D E +++E R FI N + Sbjct: 280 CTISQGDDL--WASSAKINLAIESFTLPTRVYGSSCYRMKDIESKLQSEGRGFIHNLNFK 337 Query: 236 AATAGS 241 A S Sbjct: 338 AKNYSS 343 >SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 923 Score = 29.9 bits (64), Expect = 1.6 Identities = 15/48 (31%), Positives = 22/48 (45%) Query: 808 DSHGNKFHVAVFSRVGVLVTEFECPYVKFINSLDLRKLDFVQYSKLFY 855 D H + + +L F Y FI +D R+L+F + SK FY Sbjct: 304 DIHATFIQKLNYDLINLLYEPFHNIYSSFIKPIDDRRLEFDEQSKSFY 351 >SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 306 Score = 29.5 bits (63), Expect = 2.1 Identities = 14/49 (28%), Positives = 23/49 (46%), Gaps = 4/49 (8%) Query: 15 SLGDEGSLESNDN----GKHEQDCKICDNKLCSPRVLSCLHVFCEACID 59 S+ DE LE + + + C +C + P C H+FC +CI+ Sbjct: 233 SITDERDLEDKNKLPFIPEGNRKCSLCMEFIHCPAATECGHIFCWSCIN 281 >SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 28.7 bits (61), Expect = 3.6 Identities = 13/40 (32%), Positives = 18/40 (45%) Query: 18 DEGSLESNDNGKHEQDCKICDNKLCSPRVLSCLHVFCEAC 57 +EG +++ C IC SP +C H FCE C Sbjct: 238 EEGMVKNEKKEDIPFVCLICKKDYRSPIATTCGHHFCEQC 277 >SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 468 Score = 28.3 bits (60), Expect = 4.8 Identities = 12/37 (32%), Positives = 16/37 (43%) Query: 22 LESNDNGKHEQDCKICDNKLCSPRVLSCLHVFCEACI 58 LE+ K +C IC L P C H +C C+ Sbjct: 73 LETFQKIKKTLECPICTEALQRPFTTHCGHTYCYECL 109 >SPBP35G2.08c |air1||zinc knuckle TRAMP complex subunit Air1|Schizosaccharomyces pombe|chr 2|||Manual Length = 313 Score = 27.9 bits (59), Expect = 6.3 Identities = 15/44 (34%), Positives = 20/44 (45%), Gaps = 5/44 (11%) Query: 108 STMDQSVVCTCCKSKEPAVARCTDCSHFLCSNCNSA--HEFMRC 149 S +S+VC CK DC H LC+ C + H +RC Sbjct: 81 SDPSESIVCHNCKGNGHI---SKDCPHVLCTTCGAIDDHISVRC 121 >SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 425 Score = 27.9 bits (59), Expect = 6.3 Identities = 10/26 (38%), Positives = 13/26 (50%) Query: 127 ARCTDCSHFLCSNCNSAHEFMRCFEN 152 +RC HFL CNS H + C + Sbjct: 353 SRCDTKYHFLGHKCNSCHSYNTCISS 378 >SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe|chr 1|||Manual Length = 734 Score = 27.9 bits (59), Expect = 6.3 Identities = 17/30 (56%), Positives = 17/30 (56%), Gaps = 1/30 (3%) Query: 332 MLNKTVTSQFARLLEGAPEFDVNYSVEFIS 361 ML KT SQF LEG DVN S EF S Sbjct: 452 MLKKTCGSQFTHSLEGMFR-DVNISKEFTS 480 >SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 269 Score = 27.5 bits (58), Expect = 8.3 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 13/54 (24%) Query: 34 CKICDNKLCSPRVLSCL---HVFCEACIDKLMVNEAGDSLKFDLAVECPICKQE 84 C IC L SP LSC H+FC CI + A +CP+C+++ Sbjct: 206 CVIC---LDSPENLSCTPCGHIFCNFCILSALGTTAATQ-------KCPVCRRK 249 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.321 0.136 0.412 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,171,506 Number of Sequences: 5004 Number of extensions: 120190 Number of successful extensions: 302 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 286 Number of HSP's gapped (non-prelim): 23 length of query: 857 length of database: 2,362,478 effective HSP length: 79 effective length of query: 778 effective length of database: 1,967,162 effective search space: 1530452036 effective search space used: 1530452036 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 58 (27.5 bits)
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