BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000958-TA|BGIBMGA000958-PA|IPR002524|Cation efflux protein, IPR009061|Putative DNA binding (436 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_20432| Best HMM Match : No HMM Matches (HMM E-Value=.) 130 2e-30 SB_58769| Best HMM Match : XPA_C (HMM E-Value=0.01) 41 0.002 SB_47418| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.0 SB_58080| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 3.2 SB_14471| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 3.2 SB_25840| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 4.2 SB_50434| Best HMM Match : XPA_C (HMM E-Value=2.1e-05) 29 7.4 SB_8709| Best HMM Match : Sugar_tr (HMM E-Value=0) 29 9.8 >SB_20432| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 403 Score = 130 bits (315), Expect = 2e-30 Identities = 62/124 (50%), Positives = 88/124 (70%) Query: 216 VLGGAVVSEGATLMVALSAIRKGAREANMSLYEYVMRSSDPSVNVVLLEDTAAVAGVIVA 275 +LGG+ V EGATL+ A++ +RK A E+ MS EY++R DP+ VLLED AAV GV + Sbjct: 195 ILGGSFVLEGATLLAAVTQVRKSANESGMSFREYLLRGRDPTAIAVLLEDGAAVTGVSLG 254 Query: 276 ASCMAISQYTGNPLPDALGSILVGTILGGVASFIILSNVGALVGRSIPHEQLDEINSVLE 335 A C+ ++ YTG+P+ DA+GS+++G +LG VA F+I N L+GRSIP E+L +I VLE Sbjct: 255 AVCLGLTSYTGSPVFDAIGSLMIGGLLGTVAFFLIRRNADFLIGRSIPPERLRQIIEVLE 314 Query: 336 RDFM 339 D + Sbjct: 315 SDIV 318 Score = 41.9 bits (94), Expect = 0.001 Identities = 15/44 (34%), Positives = 33/44 (75%) Query: 379 LNALLEDMKKIDTIDDVETFLLKHGENIVDMLGGEIDRIELKLR 422 L + + +++K+ T ++E F+L++GE ++D+LG ++DRIE ++ Sbjct: 313 LESDIVEIQKLTTTAEMEQFMLEYGEQVIDVLGQQVDRIERNIK 356 Score = 38.3 bits (85), Expect = 0.012 Identities = 20/40 (50%), Positives = 24/40 (60%), Gaps = 3/40 (7%) Query: 118 NFLFKLC---AWFYTGSHSLFSECIHSLADTVNQLILAYG 154 NF LC A+ YTGS ++ SE IHSLAD +NQ G Sbjct: 158 NFAVMLCKFGAYVYTGSATMLSESIHSLADLLNQACAILG 197 >SB_58769| Best HMM Match : XPA_C (HMM E-Value=0.01) Length = 123 Score = 40.7 bits (91), Expect = 0.002 Identities = 18/49 (36%), Positives = 31/49 (63%), Gaps = 1/49 (2%) Query: 3 AMTEYLLKQSDLESLPKVLRRSPY-ESEPPITVYYRRDVEAKAIEIWGS 50 A +YLL DL+ L + +P +S PP+ ++ +V++KA+E+WGS Sbjct: 25 AKRDYLLSDEDLKELNHETKDNPINKSYPPMKIFKLSEVKSKAVEVWGS 73 >SB_47418| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 635 Score = 31.9 bits (69), Expect = 1.0 Identities = 26/107 (24%), Positives = 52/107 (48%), Gaps = 4/107 (3%) Query: 284 YTGNPLPDALGSILVGTILGGVASFIILSNVGALVGRSIPHEQLDE-INSVLERDFMI-R 341 + +PD + ++++G+ +GG+ +L+ G + H+Q ++ E+ F Sbjct: 77 FVAKKIPDEVDAVVIGSGIGGMTCASLLAKTGKKILVLEQHDQAGGCCHTFHEKGFEFDS 136 Query: 342 AIHDVKGIDIGSNLIRYKAEVDFDGRALTRSYLEKHDLNALLEDMKK 388 IH + G + ++ I++ + DG+ L S LEKH + DM K Sbjct: 137 GIHYI-GEMMSNSAIKFLIDQITDGQLLW-SPLEKHYDTVAIGDMAK 181 >SB_58080| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 687 Score = 30.3 bits (65), Expect = 3.2 Identities = 21/88 (23%), Positives = 40/88 (45%), Gaps = 1/88 (1%) Query: 142 LADTVNQLILAYGIHKSVQMADPDHPYGYTNMRYVSSLISGVGIFCVGSGLSFYHGVTGI 201 L + ILA ++ M+ D YGY N+R++ L+S + L + + Sbjct: 49 LGQEIRNDILAKVVNSEGLMSLLDDCYGYDNLRFLERLLSKANCLDLVEMLRDWKSSSNC 108 Query: 202 LDPQPLHDF-YWAYFVLGGAVVSEGATL 228 L+P+ +F Y+ ++ +E A+L Sbjct: 109 LNPRLFEEFGYFPLWIKVNCTFNEFASL 136 >SB_14471| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 327 Score = 30.3 bits (65), Expect = 3.2 Identities = 19/75 (25%), Positives = 36/75 (48%), Gaps = 1/75 (1%) Query: 132 HSLFSECIHSLADTVNQLILAYGIHKSVQMADPDHPYGYTNMRYVSSLISGVGIFCVGSG 191 H+ S I + ++V+ L A + + + + P H +T R VS++++ V +F G Sbjct: 97 HAAISVTISLMFNSVSNLS-AISLDRYLAIETPLHYRAFTTKRRVSAIVAFVWVFSTTVG 155 Query: 192 LSFYHGVTGILDPQP 206 Y G + P+P Sbjct: 156 FIPYMGWRKVRRPEP 170 >SB_25840| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 768 Score = 29.9 bits (64), Expect = 4.2 Identities = 20/65 (30%), Positives = 34/65 (52%), Gaps = 1/65 (1%) Query: 98 VGLKTRSGRVVLTAIGINGCNFLFKLCAWFYTGSHSLFSECIHSLADTVNQLILAY-GIH 156 +GL ++ +VV A ++ FL L A+F S S E + +A + +Q I+ G+ Sbjct: 662 IGLSHKTVKVVTVARIVSDNAFLASLLAYFTKLSSSGSKETVRKVAKSPSQTIVGMDGLQ 721 Query: 157 KSVQM 161 KS Q+ Sbjct: 722 KSAQL 726 >SB_50434| Best HMM Match : XPA_C (HMM E-Value=2.1e-05) Length = 830 Score = 29.1 bits (62), Expect = 7.4 Identities = 10/31 (32%), Positives = 21/31 (67%) Query: 21 LRRSPYESEPPITVYYRRDVEAKAIEIWGSK 51 L+++P+ + + +Y + V A+A E+WGS+ Sbjct: 96 LKKNPHYARGDMKLYLKSQVIARAYEVWGSE 126 >SB_8709| Best HMM Match : Sugar_tr (HMM E-Value=0) Length = 493 Score = 28.7 bits (61), Expect = 9.8 Identities = 21/85 (24%), Positives = 39/85 (45%), Gaps = 2/85 (2%) Query: 223 SEGATLMVALSAIRKGAREANMSLYEYVMRSSDPSVNVVLLEDTAAVAGVIVAAS-CMAI 281 SE +L+ S +G M+LY Y++ + DT V+G ++ S + Sbjct: 25 SEKVSLIRDGSPASQGKEGEKMTLYVYMLTFFAAIGGFLFGYDTGVVSGAMILISEVFHL 84 Query: 282 SQYTGNPLPDA-LGSILVGTILGGV 305 S + + +G+ +VG +LGG+ Sbjct: 85 SDFWHELIVSGTIGTAIVGAVLGGI 109 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.322 0.139 0.405 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,200,586 Number of Sequences: 59808 Number of extensions: 503636 Number of successful extensions: 1205 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 1198 Number of HSP's gapped (non-prelim): 12 length of query: 436 length of database: 16,821,457 effective HSP length: 84 effective length of query: 352 effective length of database: 11,797,585 effective search space: 4152749920 effective search space used: 4152749920 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 61 (28.7 bits)
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