Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000944-TA|BGIBMGA000944-PA|IPR001436|Alpha crystallin,
IPR002068|Heat shock protein Hsp20, IPR008978|HSP20-like chaperone
(187 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 36 0.017
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 35 0.029
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 30 1.1
At4g40060.1 68417.m05672 homeobox-leucine zipper protein 16 (HB-... 29 1.5
At2g22430.1 68415.m02660 homeobox-leucine zipper protein 6 (HB-6... 29 2.6
At1g65010.1 68414.m07368 expressed protein similar to endosome-a... 29 2.6
At1g79940.1 68414.m09342 DNAJ heat shock N-terminal domain-conta... 28 4.5
At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identica... 28 4.5
At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identica... 28 4.5
At5g18230.1 68418.m02139 transcription regulator NOT2/NOT3/NOT5 ... 27 7.8
At5g07170.1 68418.m00817 hypothetical protein 27 7.8
At5g02760.1 68418.m00218 protein phosphatase 2C family protein /... 27 7.8
At5g02100.1 68418.m00131 oxysterol-binding family protein simila... 27 7.8
At2g40020.2 68415.m04917 expressed protein 27 7.8
At2g40020.1 68415.m04918 expressed protein 27 7.8
At2g20950.4 68415.m02474 expressed protein 27 7.8
At2g20950.3 68415.m02473 expressed protein 27 7.8
At2g20950.2 68415.m02472 expressed protein 27 7.8
At2g20950.1 68415.m02471 expressed protein 27 7.8
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 27 7.8
At2g12940.1 68415.m01419 expressed protein 27 7.8
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 35.9 bits (79), Expect = 0.017
Identities = 26/77 (33%), Positives = 42/77 (54%), Gaps = 10/77 (12%)
Query: 104 DVSQYTPEEIVVKTVDNKLLV-----HAKHEEKSDT-KSVYR---EYNREFLLPKGTNPE 154
D+ EE+ V+ D+ +L H + EEK DT V R +++R+F LP+ +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 155 AIKSSLSRDGVLTVEAP 171
+K+S+ +GVLTV P
Sbjct: 124 QVKASM-ENGVLTVTVP 139
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 35.1 bits (77), Expect = 0.029
Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 5/77 (6%)
Query: 99 LKLRFDVSQYTPEEIVVKTVDNKLLV--HAKHEEKSDTKS--VYREYNREFLLPKGTNPE 154
+K+RFD+ + E++ + DN L++ K E+ D+ S Y LP +
Sbjct: 138 IKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKD 197
Query: 155 AIKSSLSRDGVLTVEAP 171
IK+ L ++GVL + P
Sbjct: 198 KIKAEL-KNGVLFITIP 213
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 29.9 bits (64), Expect = 1.1
Identities = 25/77 (32%), Positives = 40/77 (51%), Gaps = 10/77 (12%)
Query: 104 DVSQYTPEEIVVKTVDNKLLVHA-----KHEEKSDT-KSVYR---EYNREFLLPKGTNPE 154
D+ EE+ V+ D +L + ++EEK+D V R ++ R F LP+ E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 155 AIKSSLSRDGVLTVEAP 171
IK+S+ +GVL+V P
Sbjct: 126 EIKASM-ENGVLSVTVP 141
>At4g40060.1 68417.m05672 homeobox-leucine zipper protein 16 (HB-16)
/ HD-ZIP transcription factor 16 identical to
homeodomain leucine-zipper protein ATHB-16 (GP:5668909|)
{Arabidopsis thaliana}
Length = 294
Score = 29.5 bits (63), Expect = 1.5
Identities = 13/44 (29%), Positives = 26/44 (59%), Gaps = 3/44 (6%)
Query: 16 DFSVIDTEFSSIRERFDAEMR---KMEEEMSKFRSELMNRESNN 56
D+ V+ ++ S+R FD+ R + +E+SK ++++ E NN
Sbjct: 119 DYGVLKGQYDSLRHNFDSLRRDNDSLLQEISKIKAKVNGEEDNN 162
>At2g22430.1 68415.m02660 homeobox-leucine zipper protein 6 (HB-6) /
HD-ZIP transcription factor 6 identical to
homeobox-leucine zipper protein ATHB-6 (HD-ZIP protein
ATHB-6) (SP:P46668) [Arabidopsis thaliana]
Length = 311
Score = 28.7 bits (61), Expect = 2.6
Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Query: 16 DFSVIDTEFSSIRERFDAEMRKME---EEMSKFRSEL 49
D+ V+ T++ S+R FD+ R E +E+SK +++L
Sbjct: 122 DYGVLKTQYDSLRHNFDSLRRDNESLLQEISKLKTKL 158
>At1g65010.1 68414.m07368 expressed protein similar to
endosome-associated protein (GI:1016368) [Homo sapiens];
similar to Centromeric protein E (CENP-E protein)
(Swiss-Prot:Q02224) [Homo sapiens]
Length = 1318
Score = 28.7 bits (61), Expect = 2.6
Identities = 16/49 (32%), Positives = 29/49 (59%), Gaps = 1/49 (2%)
Query: 6 LKRNIPIKLGDFSVIDTEFSSIRERFDAEMRKMEEEMSKFRSELMNRES 54
LK ++ K D + E SS+RE + + K+ EE+SK + L+++E+
Sbjct: 587 LKESLLDKEEDLKNVTAEISSLREWEGSVLEKI-EELSKVKESLVDKET 634
>At1g79940.1 68414.m09342 DNAJ heat shock N-terminal
domain-containing protein / sec63 domain-containing
protein similar to SP|Q9UGP8 Translocation protein SEC63
homolog {Homo sapiens}; contains Pfam profiles PF00226
DnaJ domain, PF02889 Sec63 domain
Length = 702
Score = 27.9 bits (59), Expect = 4.5
Identities = 10/36 (27%), Positives = 21/36 (58%)
Query: 10 IPIKLGDFSVIDTEFSSIRERFDAEMRKMEEEMSKF 45
IP++ D + F S+R + +++ M++E +KF
Sbjct: 258 IPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKF 293
>At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identical
to auxin response factor 1 GI:2245378 from [Arabidopsis
thaliana]
Length = 662
Score = 27.9 bits (59), Expect = 4.5
Identities = 12/28 (42%), Positives = 19/28 (67%)
Query: 108 YTPEEIVVKTVDNKLLVHAKHEEKSDTK 135
YTPEE+ + NKL V+A+ + K+D +
Sbjct: 619 YTPEEVKKLSPKNKLAVNARMQLKADAE 646
>At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identical
to auxin response factor 1 GI:2245378 from [Arabidopsis
thaliana]
Length = 665
Score = 27.9 bits (59), Expect = 4.5
Identities = 12/28 (42%), Positives = 19/28 (67%)
Query: 108 YTPEEIVVKTVDNKLLVHAKHEEKSDTK 135
YTPEE+ + NKL V+A+ + K+D +
Sbjct: 622 YTPEEVKKLSPKNKLAVNARMQLKADAE 649
>At5g18230.1 68418.m02139 transcription regulator NOT2/NOT3/NOT5
family protein contains Pfam domain PF04153: NOT2 /
NOT3 / NOT5 family
Length = 843
Score = 27.1 bits (57), Expect = 7.8
Identities = 14/61 (22%), Positives = 33/61 (54%), Gaps = 1/61 (1%)
Query: 19 VIDTEFSSIRERFDAEMRKMEEEMSKFRSELMNRESNNFFKXXXXXXXXXQH-SDSRQLA 77
V DT+ + +E+F+A+++K +++ ++R ++ ++ K Q D+R+L
Sbjct: 32 VYDTDNVNQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQSLVDARKLI 91
Query: 78 E 78
E
Sbjct: 92 E 92
>At5g07170.1 68418.m00817 hypothetical protein
Length = 542
Score = 27.1 bits (57), Expect = 7.8
Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 2/46 (4%)
Query: 124 VHAKHEEK--SDTKSVYREYNREFLLPKGTNPEAIKSSLSRDGVLT 167
+H ++EEK K V +YN +PK T+ K+ L ++ LT
Sbjct: 230 IHLRYEEKHLEIPKPVSDQYNAPKDVPKATHKSKTKTYLRKNSTLT 275
>At5g02760.1 68418.m00218 protein phosphatase 2C family protein /
PP2C family protein similar to Ser/Thr protein
phosphatase 2C (PP2C6) (GI:15020818) [Arabidopsis
thaliana]; similar to protein phosphatase 2C
(GI:3608412) [Mesembryanthemum crystallinum]; contains
Pfam PF00481 : Protein phosphatase 2C domain
Length = 370
Score = 27.1 bits (57), Expect = 7.8
Identities = 17/36 (47%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Query: 140 EYNREFLLPKGTNPEAI-KSSLSRDGVLTVEAPLPQ 174
E+NRE LLPK PE K LS D +T+ PQ
Sbjct: 232 EFNREPLLPKFRLPEHFTKPILSADPSVTITRLSPQ 267
>At5g02100.1 68418.m00131 oxysterol-binding family protein similar
to SWH1 [Saccharomyces cerevisiae] GI:402658; contains
Pfam profile PF01237: Oxysterol-binding protein
Length = 453
Score = 27.1 bits (57), Expect = 7.8
Identities = 16/45 (35%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Query: 2 ADSGLKRN-IPIKLGDFSVIDTEFSSIRERFDAEMRKMEEEMSKF 45
+DS L+ + +++GD S E SS+ +R AE + EE+ KF
Sbjct: 350 SDSRLRPDRYALEMGDMSKSGFEKSSLEDRQRAEKKSREEKGQKF 394
>At2g40020.2 68415.m04917 expressed protein
Length = 182
Score = 27.1 bits (57), Expect = 7.8
Identities = 14/46 (30%), Positives = 22/46 (47%)
Query: 111 EEIVVKTVDNKLLVHAKHEEKSDTKSVYREYNREFLLPKGTNPEAI 156
EE+ + N+L V EEK + ++ EY R+ N EA+
Sbjct: 87 EEMAEEEQPNQLAVEIHEEEKEQEQDMHIEYGRDQADELSINSEAV 132
>At2g40020.1 68415.m04918 expressed protein
Length = 228
Score = 27.1 bits (57), Expect = 7.8
Identities = 14/46 (30%), Positives = 22/46 (47%)
Query: 111 EEIVVKTVDNKLLVHAKHEEKSDTKSVYREYNREFLLPKGTNPEAI 156
EE+ + N+L V EEK + ++ EY R+ N EA+
Sbjct: 75 EEMAEEEQPNQLAVEIHEEEKEQEQDMHIEYGRDQADELSINSEAV 120
>At2g20950.4 68415.m02474 expressed protein
Length = 530
Score = 27.1 bits (57), Expect = 7.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Query: 27 IRERFDAEMRKMEEEMSKFRSELMNRE 53
+RE D EM ++EM + R EL RE
Sbjct: 455 VREECDREMTAGKKEMEEMREELRRRE 481
>At2g20950.3 68415.m02473 expressed protein
Length = 505
Score = 27.1 bits (57), Expect = 7.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Query: 27 IRERFDAEMRKMEEEMSKFRSELMNRE 53
+RE D EM ++EM + R EL RE
Sbjct: 430 VREECDREMTAGKKEMEEMREELRRRE 456
>At2g20950.2 68415.m02472 expressed protein
Length = 503
Score = 27.1 bits (57), Expect = 7.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Query: 27 IRERFDAEMRKMEEEMSKFRSELMNRE 53
+RE D EM ++EM + R EL RE
Sbjct: 428 VREECDREMTAGKKEMEEMREELRRRE 454
>At2g20950.1 68415.m02471 expressed protein
Length = 520
Score = 27.1 bits (57), Expect = 7.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Query: 27 IRERFDAEMRKMEEEMSKFRSELMNRE 53
+RE D EM ++EM + R EL RE
Sbjct: 445 VREECDREMTAGKKEMEEMREELRRRE 471
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 27.1 bits (57), Expect = 7.8
Identities = 12/26 (46%), Positives = 17/26 (65%)
Query: 28 RERFDAEMRKMEEEMSKFRSELMNRE 53
R++ + E RK EEEM+K R E R+
Sbjct: 625 RKKREEEARKREEEMAKIREEERQRK 650
>At2g12940.1 68415.m01419 expressed protein
Length = 294
Score = 27.1 bits (57), Expect = 7.8
Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Query: 79 PSHWDSLNSPLIQDEGDGKTLKLRFDVSQYTPEEIVVKTVDNKL 122
PS ++ +S L+ +GD +L FD + YT +E+ NKL
Sbjct: 101 PSPGNTPSSRLV--DGDQNASRLEFDANDYTDDELNKIAKSNKL 142
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.313 0.130 0.361
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,222,353
Number of Sequences: 28952
Number of extensions: 169867
Number of successful extensions: 468
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 453
Number of HSP's gapped (non-prelim): 26
length of query: 187
length of database: 12,070,560
effective HSP length: 77
effective length of query: 110
effective length of database: 9,841,256
effective search space: 1082538160
effective search space used: 1082538160
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 57 (27.1 bits)
- SilkBase 1999-2023 -