BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000938-TA|BGIBMGA000938-PA|IPR006575|RWD, IPR010541|Protein of unknown function DUF1115, IPR013881|Pre-mRNA-splicing factor 3, IPR004856|ALG6, ALG8 glycosyltransferase (537 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17C9.07 |alg8||glucosyltransferase Alg8|Schizosaccharomyces ... 109 1e-24 SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosac... 59 1e-09 SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 32 0.17 SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 32 0.17 SPCC14G10.01 ||SPCC18B5.12|dephospho-CoA kinase |Schizosaccharom... 31 0.40 SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 31 0.53 SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 30 0.93 SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces ... 29 2.2 SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 28 2.8 SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 28 3.8 SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 27 5.0 SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pomb... 27 5.0 SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|ch... 27 5.0 SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar... 27 6.6 >SPAC17C9.07 |alg8||glucosyltransferase Alg8|Schizosaccharomyces pombe|chr 1|||Manual Length = 501 Score = 109 bits (261), Expect = 1e-24 Identities = 53/95 (55%), Positives = 69/95 (72%), Gaps = 3/95 (3%) Query: 177 PSH-STDFEVHRNWLAITNNVSVEEWYYESTSEWTLDYPPLFAWLEYGLSIVAKY--FDP 233 PS+ STDFEVHRNWLAIT+++ + EWY S SEWTLDYPP FA++E LS +A + FD Sbjct: 23 PSYRSTDFEVHRNWLAITHSLPISEWYKSSISEWTLDYPPFFAYMECVLSWIAYFFGFDK 82 Query: 234 KMIKIENLNYKSDMAVLFQRLSVIFLDFVYIFSVK 268 M+ NLNY S V+FQR SVI L+ V +F+++ Sbjct: 83 AMLDPYNLNYVSPSTVVFQRGSVIVLELVLLFALR 117 Score = 103 bits (247), Expect = 6e-23 Identities = 76/230 (33%), Positives = 113/230 (49%), Gaps = 19/230 (8%) Query: 287 YGINIPKSEASMTGGLVQEYDHSVLPSITPSFTFIMTCLSM-MPALYKMWCLCADRRYRP 345 Y +N S + T GLV E +VLP+I P+ TF + CL + + L K++ R Sbjct: 287 YALNQGTSINAPTRGLVGESSFAVLPNIPPALTFYI-CLGLQITVLIKLFIKPTWR---- 341 Query: 346 ICFIRCIVVCATCSFMLGWHVHEKAILMIIIPLSFLSVLGDVDGRLFILLSTVGHYSLFP 405 F+ + +C SF+ GWHVHEKAILM+I+P S LS++ F L+ G+ SL P Sbjct: 342 -VFVGAVTLCGWISFLFGWHVHEKAILMVILPFSILSLIDRRYLEAFRPLAVSGYLSLLP 400 Query: 406 LLYPKDLLSIKIFLLLTHVAIVFGNVPALYTVETKATKKRRRRFMRLPMIGPFESIYIYG 465 LL+ + IK +A+ L T + A +R F ++ YI G Sbjct: 401 LLFTLNEAPIKYLFTGAWIAM-------LLTFDKCAPVPVKRVF----LLNRVNIAYISG 449 Query: 466 LILLCVYENMLHVAWGLDKTLPFLPLMMTSTYCALGVFYFWIKYYHYFLT 515 + L +Y +H DK FLPLM+ STY A G+F+ ++ + T Sbjct: 450 FVPLFIYNCFIHKLVMGDK-FEFLPLMLLSTYAAWGIFWSFVSLLWLYFT 498 >SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosaccharomyces pombe|chr 2|||Manual Length = 506 Score = 59.3 bits (137), Expect = 1e-09 Identities = 29/97 (29%), Positives = 49/97 (50%), Gaps = 2/97 (2%) Query: 182 DFEVHRNWLAITNNVSVEEWYYESTSEWTLDYPPLFAWLEYGLSIVAKY-FDPK-MIKIE 239 DFE R+W+ +T + V +WY+ W LDYPPL A++ + I+ Y F+P+ + Sbjct: 58 DFEAQRHWMELTLHTPVSQWYFRDLQWWGLDYPPLTAYVSWFFGIIGHYFFNPEWFADVT 117 Query: 240 NLNYKSDMAVLFQRLSVIFLDFVYIFSVKSIYFIVWT 276 + ++S LF R +VI + + Y W+ Sbjct: 118 SRGFESLELKLFMRSTVIASHLLILVPPLMFYSKWWS 154 Score = 40.3 bits (90), Expect = 7e-04 Identities = 22/63 (34%), Positives = 40/63 (63%), Gaps = 2/63 (3%) Query: 360 FMLGWHVHEKAILMIIIPLSFLSVLGDVDGRLFI-LLSTVGHYSLFPLLYPKDLLSIKIF 418 F+ + VHEK++L+ ++P S L G++ + +I L + + +SL+PLL KD L ++ F Sbjct: 356 FLFSFQVHEKSVLLPLLPTSILLCHGNITTKPWIALANNLAVFSLWPLL-KKDGLGLQYF 414 Query: 419 LLL 421 L+ Sbjct: 415 TLV 417 >SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 1887 Score = 32.3 bits (70), Expect = 0.17 Identities = 47/220 (21%), Positives = 87/220 (39%), Gaps = 8/220 (3%) Query: 135 ENDRKFTNFEELQFQSNNNTKFSNMKPNWPVAVLHLEAAAKQP-SHSTDFEVHRNWLAIT 193 E+D N + +NNN +N + +A L L A K DF + L I Sbjct: 526 EDDNDNDNDNDRDSNNNNNNNNTNTDDDDKLAYLELHEALKLAFLQQYDFSKNVQDLEIM 585 Query: 194 NNVSVEEWYYESTSEWTLDYPPLFAWLEYGLSIVAKYFDPKMIKIENLNYKSDMAVLFQR 253 ++ + + TS++ + FA L Y +VA ++I + ++ S + FQ Sbjct: 586 EFLACMSLHKDGTSKYAYEISACFAPLIYTCRLVAACELQRLIDEKQIDLLSIPS--FQT 643 Query: 254 LSVIFLDFVYIF---SVKSIYFIVWTIFRPFESVTK-YGINIPKSEASMTGGLVQEYDHS 309 I V+ F +++Y +++ + + + G S + L Q +S Sbjct: 644 AGSIAYAHVFCFITLGQRNLYDVLYETQKVVRDIIRTEGYANTLQGLSPSTVLFQPRSNS 703 Query: 310 VLPSITPSF-TFIMTCLSMMPALYKMWCLCADRRYRPICF 348 + P I +F + LS + ALY+ + +CF Sbjct: 704 MYPCIGDAFNNMVRLDLSELTALYEGMFAKVQDLLKELCF 743 >SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 32.3 bits (70), Expect = 0.17 Identities = 47/220 (21%), Positives = 87/220 (39%), Gaps = 8/220 (3%) Query: 135 ENDRKFTNFEELQFQSNNNTKFSNMKPNWPVAVLHLEAAAKQP-SHSTDFEVHRNWLAIT 193 E+D N + +NNN +N + +A L L A K DF + L I Sbjct: 526 EDDNDNDNDNDRDSNNNNNNNNTNTDDDDKLAYLELHEALKLAFLQQYDFSKNVQDLEIM 585 Query: 194 NNVSVEEWYYESTSEWTLDYPPLFAWLEYGLSIVAKYFDPKMIKIENLNYKSDMAVLFQR 253 ++ + + TS++ + FA L Y +VA ++I + ++ S + FQ Sbjct: 586 EFLACMSLHKDGTSKYAYEISACFAPLIYTCRLVAACELQRLIDEKQIDLLSIPS--FQT 643 Query: 254 LSVIFLDFVYIF---SVKSIYFIVWTIFRPFESVTK-YGINIPKSEASMTGGLVQEYDHS 309 I V+ F +++Y +++ + + + G S + L Q +S Sbjct: 644 AGSIAYAHVFCFITLGQRNLYDVLYETQKVVRDIIRTEGYANTLQGLSPSTVLFQPRSNS 703 Query: 310 VLPSITPSF-TFIMTCLSMMPALYKMWCLCADRRYRPICF 348 + P I +F + LS + ALY+ + +CF Sbjct: 704 MYPCIGDAFNNMVRLDLSELTALYEGMFAKVQDLLKELCF 743 >SPCC14G10.01 ||SPCC18B5.12|dephospho-CoA kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 236 Score = 31.1 bits (67), Expect = 0.40 Identities = 12/22 (54%), Positives = 16/22 (72%) Query: 308 HSVLPSITPSFTFIMTCLSMMP 329 H+VLP ITPS+ F + CL + P Sbjct: 191 HNVLPLITPSYFFTLLCLILPP 212 >SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces pombe|chr 2|||Manual Length = 2493 Score = 30.7 bits (66), Expect = 0.53 Identities = 22/65 (33%), Positives = 36/65 (55%), Gaps = 2/65 (3%) Query: 204 ESTSEWTLDYPPLFAWLEYGLSIVAKYFDPKMIKI-ENLNYKSDMAVLFQRLSVIFLDFV 262 E+T+EW L+Y L +WL+ L+ K + + KI E++ Y + RLSV L F Sbjct: 2206 EATNEWELNYFGLQSWLKLVLADPKKSCEKEFSKIWESMRYLILFKHAWVRLSVSRL-FG 2264 Query: 263 YIFSV 267 + F++ Sbjct: 2265 HFFAI 2269 >SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 29.9 bits (64), Expect = 0.93 Identities = 32/103 (31%), Positives = 48/103 (46%), Gaps = 6/103 (5%) Query: 232 DPKMIKIENLNYKSDMAVLFQRLSVIFLDFVYIFSVKSIYFIVWTIFRPFESVTKYGINI 291 D +I+ ++L Y S +A L + I L I + +Y I R +T N Sbjct: 809 DALLIQKKSLTYSSFLAFLVKLCLDIVLTPQNIKTSTDVYIIQTWALRLLYQITSIWENP 868 Query: 292 PKSEASMTGG---LVQEYDHSVLPSITPSFTFIMTCLSMMPAL 331 K E+S+ G LVQE + S PS T +FI++ L M+ L Sbjct: 869 LKFESSLIVGLFALVQEVERS--PSSTYR-SFILSLLQMLEFL 908 >SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1150 Score = 28.7 bits (61), Expect = 2.2 Identities = 15/44 (34%), Positives = 18/44 (40%) Query: 105 TKDCEDWWREIKCLNWQKLVIQKSEVLECLENDRKFTNFEELQF 148 T D E W +KCL W L S L+ E R F+ F Sbjct: 172 TPDAEGWNEFLKCLEWAVLSSLDSRFLQLSEQIRSIKLFDSSIF 215 >SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 28.3 bits (60), Expect = 2.8 Identities = 14/45 (31%), Positives = 26/45 (57%), Gaps = 1/45 (2%) Query: 145 ELQFQSNNNTKFSNMKPNWPVAVLHL-EAAAKQPSHSTDFEVHRN 188 E+ SN+++K + PN+ + ++ ++AK HS D + HRN Sbjct: 11 EMPSNSNSSSKLNAKSPNFIPSSSNIPRSSAKTKEHSADRKPHRN 55 >SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein Mug36|Schizosaccharomyces pombe|chr 2|||Manual Length = 1646 Score = 27.9 bits (59), Expect = 3.8 Identities = 23/74 (31%), Positives = 36/74 (48%), Gaps = 3/74 (4%) Query: 231 FDPKMIKIENLNYKSDMAVLFQRLSV--IFLDFVYIFSV-KSIYFIVWTIFRPFESVTKY 287 F+ K+ +I+ N +D+ L S IF+D V + S KS+ IF+ E + Sbjct: 422 FEMKINQIQIFNDNNDIDELTMTFSDLDIFMDSVTLVSFGKSLQSPCSLIFKKLERIVSL 481 Query: 288 GINIPKSEASMTGG 301 I+IP E S+ G Sbjct: 482 FIHIPGGEISLPLG 495 >SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22 |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 27.5 bits (58), Expect = 5.0 Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Query: 118 LNWQKLVIQKSEVLECLENDRKFTNFEELQFQSNNNTKFSNM 159 +N +KL + K+++ + EN K L QSN T+F N+ Sbjct: 170 VNLEKLWVGKNKITK-FENFEKLQKLSLLSIQSNRITQFENL 210 >SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pombe|chr 2|||Manual Length = 955 Score = 27.5 bits (58), Expect = 5.0 Identities = 26/92 (28%), Positives = 43/92 (46%), Gaps = 5/92 (5%) Query: 226 IVAKYFDPKMIKIENLNYKSDMAVLFQRLSVIFLDFVYIFSVKSIY---FIVWTIFRPFE 282 IV + D I L+ SD+ + +R+S + +F+Y F K+I I + + FE Sbjct: 646 IVRLFQDIWPILSRTLDTFSDVLIC-ERISKLLKNFIYTFKEKAIVTLPVITEALIKGFE 704 Query: 283 SVTKYGINIPKSEASMTGGLVQEYDHSVLPSI 314 T+YG + S A + V E D L ++ Sbjct: 705 K-TQYGCFLWVSGACVRQFGVPEMDEQTLSAV 735 >SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 752 Score = 27.5 bits (58), Expect = 5.0 Identities = 17/56 (30%), Positives = 30/56 (53%), Gaps = 5/56 (8%) Query: 98 IVCIEGETKDCEDWWREIKCLNWQKLVIQKSEVLECLENDRKFTNFE-ELQFQSNN 152 I+ IEG K W+ + C + +K ++ K+ + E +E +K TN +L+F N Sbjct: 392 IIHIEGNWK----LWKGLGCPSLEKPLVDKAAIDEAVEGLKKLTNTPVKLRFAMGN 443 >SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 27.1 bits (57), Expect = 6.6 Identities = 15/47 (31%), Positives = 24/47 (51%), Gaps = 2/47 (4%) Query: 491 LMMTSTYCALGVFYFWIKYYHYFLTFNVSKVPTFGQGTSSYNIRKSK 537 +++T Y LG+F F YY Y T KV G + Y++ K++ Sbjct: 79 ILVTKAYAILGLFRFTAGYYLYLCT--ERKVVAVIGGHNVYHVDKTQ 123 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.326 0.140 0.450 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,597,571 Number of Sequences: 5004 Number of extensions: 113516 Number of successful extensions: 373 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 362 Number of HSP's gapped (non-prelim): 18 length of query: 537 length of database: 2,362,478 effective HSP length: 76 effective length of query: 461 effective length of database: 1,982,174 effective search space: 913782214 effective search space used: 913782214 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.7 bits) S2: 56 (26.6 bits)
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