BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000938-TA|BGIBMGA000938-PA|IPR006575|RWD, IPR010541|Protein of unknown function DUF1115, IPR013881|Pre-mRNA-splicing factor 3, IPR004856|ALG6, ALG8 glycosyltransferase (537 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g44660.1 68415.m05558 ALG6, ALG8 glycosyltransferase family p... 110 2e-24 At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family p... 65 1e-10 At5g60170.1 68418.m07543 RNA recognition motif (RRM)-containing ... 34 0.27 At2g32860.2 68415.m04029 glycosyl hydrolase family 1 protein 31 1.9 At1g43190.1 68414.m04977 polypyrimidine tract-binding protein, p... 30 3.4 At5g01510.1 68418.m00065 expressed protein contains Pfam domain,... 29 5.9 At3g45630.1 68416.m04928 RNA recognition motif (RRM)-containing ... 29 5.9 At4g38810.2 68417.m05495 calcium-binding EF hand family protein ... 29 7.7 >At2g44660.1 68415.m05558 ALG6, ALG8 glycosyltransferase family protein similar to SP|P40351 Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC 2.4.1.-) (Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase) {Saccharomyces cerevisiae}; contains Pfam profile PF03155: ALG6, ALG8 glycosyltransferase family Length = 383 Score = 110 bits (265), Expect = 2e-24 Identities = 46/91 (50%), Positives = 68/91 (74%), Gaps = 1/91 (1%) Query: 178 SHSTDFEVHRNWLAITNNVSVEEWYYESTSEWTLDYPPLFAWLEYGLSIVAKYFDPKMIK 237 S STDFEVHRNWLAITN++ + +WY++ TS+WTLDYPP FA+ E LSI A+ DP+++ Sbjct: 30 SRSTDFEVHRNWLAITNSLPLTKWYFDETSQWTLDYPPFFAYFERFLSIFARLVDPRIVD 89 Query: 238 IEN-LNYKSDMAVLFQRLSVIFLDFVYIFSV 267 +++ L+Y ++ + FQR+SVI D ++ V Sbjct: 90 LQSGLDYNAESVIYFQRISVIVSDLCLLYGV 120 Score = 92.3 bits (219), Expect = 7e-19 Identities = 52/124 (41%), Positives = 67/124 (54%), Gaps = 6/124 (4%) Query: 286 KYGINIPKSEASMTGGLVQEYD-HSVLPSITPSFTFIMTCLSMMPALYKMWCLCADRRYR 344 K G I AS TGGLV + +VLP ITP TF M L++ P L K W ++ Sbjct: 248 KLGYEIQIPSASFTGGLVGDSSPFAVLPQITPLTTFAMVLLAISPCLIKAW-----KKPH 302 Query: 345 PICFIRCIVVCATCSFMLGWHVHEKAILMIIIPLSFLSVLGDVDGRLFILLSTVGHYSLF 404 R + TC F+ GWHVHEKA L IPL+ ++V D + + L+S V YSL+ Sbjct: 303 SGLVARWVAYAYTCGFLFGWHVHEKASLHFTIPLAIVAVQSLEDAKHYFLVSIVSCYSLY 362 Query: 405 PLLY 408 PLLY Sbjct: 363 PLLY 366 >At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family protein similar to SP|Q9Y672 Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC 2.4.1.-) (Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase) {Homo sapiens}; contains Pfam profile PF03155: ALG6, ALG8 glycosyltransferase family Length = 533 Score = 65.3 bits (152), Expect = 1e-10 Identities = 33/103 (32%), Positives = 57/103 (55%), Gaps = 6/103 (5%) Query: 177 PSHSTDFEVHRNWLAITNNVSVEEWY----YESTSEWTLDYPPLFAWLEYGLSIVAKYFD 232 P DFE R+W+ IT N+ V +WY Y + W LDYPPL A+ Y I ++F+ Sbjct: 67 PPKFGDFEAQRHWMEITTNLPVIDWYRNGTYNDLTYWGLDYPPLTAYQSYIHGIFLRFFN 126 Query: 233 PKMIK-IENLNYKSDMAVLFQRLSVIFLDFVYIFSVKSIYFIV 274 P+ + + + ++S + L R +V+ D +IF +++F++ Sbjct: 127 PESVALLSSRGHESYLGKLLMRWTVLSSD-AFIFFPAALFFVL 168 >At5g60170.1 68418.m07543 RNA recognition motif (RRM)-containing protein low similarity to transcriptional repressor Not4-Np [Homo sapiens] GI:6856207; contains INTERPRO:IPR000504 RNA-binding region RNP-1 (RNA recognition motif) Length = 944 Score = 33.9 bits (74), Expect = 0.27 Identities = 15/31 (48%), Positives = 17/31 (54%), Gaps = 1/31 (3%) Query: 89 GFCLPGKPGIVCIEGETKDCEDWWREIKCLN 119 GF L GKP C G TK C W R + C+N Sbjct: 138 GFILDGKPLKACF-GTTKYCHAWLRNVACVN 167 >At2g32860.2 68415.m04029 glycosyl hydrolase family 1 protein Length = 614 Score = 31.1 bits (67), Expect = 1.9 Identities = 24/68 (35%), Positives = 38/68 (55%), Gaps = 8/68 (11%) Query: 174 AKQPSHSTDFEVHRNWLAITNNVSVEEWYYESTSEWTLDYPPLFAWLEYGLS-IVAKYFD 232 + Q ++ TD V NW ITNN+S+ + ++TS + YP A L+ L I +Y D Sbjct: 420 SSQLNYETDLRV--NWTVITNNLSLPD--LQTTSMGIVIYP---AGLKNILKHIKDEYMD 472 Query: 233 PKMIKIEN 240 P++ +EN Sbjct: 473 PEIYIMEN 480 >At1g43190.1 68414.m04977 polypyrimidine tract-binding protein, putative / heterogeneous nuclear ribonucleoprotein, putative similar to Polypyrimidine tract-binding protein 1 (PTB) (Heterogeneous nuclear ribonucleoprotein I) (hnRNP I) from {Rattus norvegicus} SP|Q00438, {Homo sapiens} SP|P26599, [Homo sapiens] GI:35770; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 432 Score = 30.3 bits (65), Expect = 3.4 Identities = 15/50 (30%), Positives = 24/50 (48%), Gaps = 1/50 (2%) Query: 116 KCLNWQKLVIQKSEVLE-CLENDRKFTNFEELQFQSNNNTKFSNMKPNWP 164 +C + +Q + + C + D +F+N EELQ NN+ PN P Sbjct: 149 QCAASARTALQGRNIYDGCCQLDIQFSNLEELQVNYNNDRSRDYTNPNLP 198 >At5g01510.1 68418.m00065 expressed protein contains Pfam domain, PF04884: Protein of unknown function, DUF647 Length = 509 Score = 29.5 bits (63), Expect = 5.9 Identities = 18/77 (23%), Positives = 31/77 (40%) Query: 167 VLHLEAAAKQPSHSTDFEVHRNWLAITNNVSVEEWYYESTSEWTLDYPPLFAWLEYGLSI 226 +L L K S F+V+ + + W E+ E D +F WL+ LS Sbjct: 411 ILTLNKLNKDTEFSVSFKVNATSRDVLRCLWQAYWLEENMEESFKDKDSVFHWLKQSLSE 470 Query: 227 VAKYFDPKMIKIENLNY 243 + FD + K++ + Sbjct: 471 MDNKFDDFLFKLDTAGW 487 >At3g45630.1 68416.m04928 RNA recognition motif (RRM)-containing protein similar to SP|P34909 General negative regulator of transcription subunit 4 {Saccharomyces cerevisiae}; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 989 Score = 29.5 bits (63), Expect = 5.9 Identities = 15/39 (38%), Positives = 18/39 (46%), Gaps = 1/39 (2%) Query: 89 GFCLPGKPGIVCIEGETKDCEDWWREIKCLNWQKLVIQK 127 GF L GK C G TK C W R + C N L + + Sbjct: 180 GFILDGKALKACF-GTTKYCHAWLRNVACNNQDCLYLHE 217 >At4g38810.2 68417.m05495 calcium-binding EF hand family protein contains INTERPRO:IPR002048 calcium-binding EF-hand domain Length = 375 Score = 29.1 bits (62), Expect = 7.7 Identities = 17/62 (27%), Positives = 31/62 (50%), Gaps = 1/62 (1%) Query: 121 QKLVIQKSEVLECLENDRKFTNFEELQFQSNNNTKFSNMK-PNWPVAVLHLEAAAKQPSH 179 Q+ V+ S+++E +EN++ F F E +FQ + + + AV + AA P+ Sbjct: 11 QQQVLDGSDIVELVENEKVFDKFVEQKFQQLDQDEDGKLSVTELQPAVADIGAALGLPAQ 70 Query: 180 ST 181 T Sbjct: 71 GT 72 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.326 0.140 0.450 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,976,536 Number of Sequences: 28952 Number of extensions: 545928 Number of successful extensions: 1480 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 1471 Number of HSP's gapped (non-prelim): 10 length of query: 537 length of database: 12,070,560 effective HSP length: 85 effective length of query: 452 effective length of database: 9,609,640 effective search space: 4343557280 effective search space used: 4343557280 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.7 bits) S2: 62 (29.1 bits)
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