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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000938-TA|BGIBMGA000938-PA|IPR006575|RWD,
IPR010541|Protein of unknown function DUF1115,
IPR013881|Pre-mRNA-splicing factor 3, IPR004856|ALG6, ALG8
glycosyltransferase
         (537 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g44660.1 68415.m05558 ALG6, ALG8 glycosyltransferase family p...   110   2e-24
At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family p...    65   1e-10
At5g60170.1 68418.m07543 RNA recognition motif (RRM)-containing ...    34   0.27 
At2g32860.2 68415.m04029 glycosyl hydrolase family 1 protein           31   1.9  
At1g43190.1 68414.m04977 polypyrimidine tract-binding protein, p...    30   3.4  
At5g01510.1 68418.m00065 expressed protein contains Pfam domain,...    29   5.9  
At3g45630.1 68416.m04928 RNA recognition motif (RRM)-containing ...    29   5.9  
At4g38810.2 68417.m05495 calcium-binding EF hand family protein ...    29   7.7  

>At2g44660.1 68415.m05558 ALG6, ALG8 glycosyltransferase family
           protein similar to SP|P40351 Dolichyl pyrophosphate
           Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC
           2.4.1.-) (Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl
           glucosyltransferase) {Saccharomyces cerevisiae};
           contains Pfam profile PF03155: ALG6, ALG8
           glycosyltransferase family
          Length = 383

 Score =  110 bits (265), Expect = 2e-24
 Identities = 46/91 (50%), Positives = 68/91 (74%), Gaps = 1/91 (1%)

Query: 178 SHSTDFEVHRNWLAITNNVSVEEWYYESTSEWTLDYPPLFAWLEYGLSIVAKYFDPKMIK 237
           S STDFEVHRNWLAITN++ + +WY++ TS+WTLDYPP FA+ E  LSI A+  DP+++ 
Sbjct: 30  SRSTDFEVHRNWLAITNSLPLTKWYFDETSQWTLDYPPFFAYFERFLSIFARLVDPRIVD 89

Query: 238 IEN-LNYKSDMAVLFQRLSVIFLDFVYIFSV 267
           +++ L+Y ++  + FQR+SVI  D   ++ V
Sbjct: 90  LQSGLDYNAESVIYFQRISVIVSDLCLLYGV 120



 Score = 92.3 bits (219), Expect = 7e-19
 Identities = 52/124 (41%), Positives = 67/124 (54%), Gaps = 6/124 (4%)

Query: 286 KYGINIPKSEASMTGGLVQEYD-HSVLPSITPSFTFIMTCLSMMPALYKMWCLCADRRYR 344
           K G  I    AS TGGLV +    +VLP ITP  TF M  L++ P L K W     ++  
Sbjct: 248 KLGYEIQIPSASFTGGLVGDSSPFAVLPQITPLTTFAMVLLAISPCLIKAW-----KKPH 302

Query: 345 PICFIRCIVVCATCSFMLGWHVHEKAILMIIIPLSFLSVLGDVDGRLFILLSTVGHYSLF 404
                R +    TC F+ GWHVHEKA L   IPL+ ++V    D + + L+S V  YSL+
Sbjct: 303 SGLVARWVAYAYTCGFLFGWHVHEKASLHFTIPLAIVAVQSLEDAKHYFLVSIVSCYSLY 362

Query: 405 PLLY 408
           PLLY
Sbjct: 363 PLLY 366


>At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family
           protein similar to SP|Q9Y672 Dolichyl pyrophosphate
           Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC 2.4.1.-)
           (Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl
           glucosyltransferase) {Homo sapiens}; contains Pfam
           profile PF03155: ALG6, ALG8 glycosyltransferase family
          Length = 533

 Score = 65.3 bits (152), Expect = 1e-10
 Identities = 33/103 (32%), Positives = 57/103 (55%), Gaps = 6/103 (5%)

Query: 177 PSHSTDFEVHRNWLAITNNVSVEEWY----YESTSEWTLDYPPLFAWLEYGLSIVAKYFD 232
           P    DFE  R+W+ IT N+ V +WY    Y   + W LDYPPL A+  Y   I  ++F+
Sbjct: 67  PPKFGDFEAQRHWMEITTNLPVIDWYRNGTYNDLTYWGLDYPPLTAYQSYIHGIFLRFFN 126

Query: 233 PKMIK-IENLNYKSDMAVLFQRLSVIFLDFVYIFSVKSIYFIV 274
           P+ +  + +  ++S +  L  R +V+  D  +IF   +++F++
Sbjct: 127 PESVALLSSRGHESYLGKLLMRWTVLSSD-AFIFFPAALFFVL 168


>At5g60170.1 68418.m07543 RNA recognition motif (RRM)-containing
           protein low similarity to transcriptional repressor
           Not4-Np [Homo sapiens] GI:6856207; contains
           INTERPRO:IPR000504 RNA-binding region RNP-1 (RNA
           recognition motif)
          Length = 944

 Score = 33.9 bits (74), Expect = 0.27
 Identities = 15/31 (48%), Positives = 17/31 (54%), Gaps = 1/31 (3%)

Query: 89  GFCLPGKPGIVCIEGETKDCEDWWREIKCLN 119
           GF L GKP   C  G TK C  W R + C+N
Sbjct: 138 GFILDGKPLKACF-GTTKYCHAWLRNVACVN 167


>At2g32860.2 68415.m04029 glycosyl hydrolase family 1 protein 
          Length = 614

 Score = 31.1 bits (67), Expect = 1.9
 Identities = 24/68 (35%), Positives = 38/68 (55%), Gaps = 8/68 (11%)

Query: 174 AKQPSHSTDFEVHRNWLAITNNVSVEEWYYESTSEWTLDYPPLFAWLEYGLS-IVAKYFD 232
           + Q ++ TD  V  NW  ITNN+S+ +   ++TS   + YP   A L+  L  I  +Y D
Sbjct: 420 SSQLNYETDLRV--NWTVITNNLSLPD--LQTTSMGIVIYP---AGLKNILKHIKDEYMD 472

Query: 233 PKMIKIEN 240
           P++  +EN
Sbjct: 473 PEIYIMEN 480


>At1g43190.1 68414.m04977 polypyrimidine tract-binding protein,
           putative / heterogeneous nuclear ribonucleoprotein,
           putative similar to Polypyrimidine tract-binding protein
           1 (PTB) (Heterogeneous nuclear ribonucleoprotein I)
           (hnRNP I) from {Rattus norvegicus} SP|Q00438, {Homo
           sapiens} SP|P26599, [Homo sapiens] GI:35770; contains
           InterPro entry IPR000504: RNA-binding region RNP-1 (RNA
           recognition motif) (RRM)
          Length = 432

 Score = 30.3 bits (65), Expect = 3.4
 Identities = 15/50 (30%), Positives = 24/50 (48%), Gaps = 1/50 (2%)

Query: 116 KCLNWQKLVIQKSEVLE-CLENDRKFTNFEELQFQSNNNTKFSNMKPNWP 164
           +C    +  +Q   + + C + D +F+N EELQ   NN+       PN P
Sbjct: 149 QCAASARTALQGRNIYDGCCQLDIQFSNLEELQVNYNNDRSRDYTNPNLP 198


>At5g01510.1 68418.m00065 expressed protein contains Pfam domain,
           PF04884: Protein of unknown function, DUF647
          Length = 509

 Score = 29.5 bits (63), Expect = 5.9
 Identities = 18/77 (23%), Positives = 31/77 (40%)

Query: 167 VLHLEAAAKQPSHSTDFEVHRNWLAITNNVSVEEWYYESTSEWTLDYPPLFAWLEYGLSI 226
           +L L    K    S  F+V+     +   +    W  E+  E   D   +F WL+  LS 
Sbjct: 411 ILTLNKLNKDTEFSVSFKVNATSRDVLRCLWQAYWLEENMEESFKDKDSVFHWLKQSLSE 470

Query: 227 VAKYFDPKMIKIENLNY 243
           +   FD  + K++   +
Sbjct: 471 MDNKFDDFLFKLDTAGW 487


>At3g45630.1 68416.m04928 RNA recognition motif (RRM)-containing
           protein similar to SP|P34909 General negative regulator
           of transcription subunit 4 {Saccharomyces cerevisiae};
           contains InterPro entry IPR000504: RNA-binding region
           RNP-1 (RNA recognition motif) (RRM)
          Length = 989

 Score = 29.5 bits (63), Expect = 5.9
 Identities = 15/39 (38%), Positives = 18/39 (46%), Gaps = 1/39 (2%)

Query: 89  GFCLPGKPGIVCIEGETKDCEDWWREIKCLNWQKLVIQK 127
           GF L GK    C  G TK C  W R + C N   L + +
Sbjct: 180 GFILDGKALKACF-GTTKYCHAWLRNVACNNQDCLYLHE 217


>At4g38810.2 68417.m05495 calcium-binding EF hand family protein
           contains INTERPRO:IPR002048 calcium-binding EF-hand
           domain
          Length = 375

 Score = 29.1 bits (62), Expect = 7.7
 Identities = 17/62 (27%), Positives = 31/62 (50%), Gaps = 1/62 (1%)

Query: 121 QKLVIQKSEVLECLENDRKFTNFEELQFQSNNNTKFSNMK-PNWPVAVLHLEAAAKQPSH 179
           Q+ V+  S+++E +EN++ F  F E +FQ  +  +   +       AV  + AA   P+ 
Sbjct: 11  QQQVLDGSDIVELVENEKVFDKFVEQKFQQLDQDEDGKLSVTELQPAVADIGAALGLPAQ 70

Query: 180 ST 181
            T
Sbjct: 71  GT 72


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.326    0.140    0.450 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,976,536
Number of Sequences: 28952
Number of extensions: 545928
Number of successful extensions: 1480
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1471
Number of HSP's gapped (non-prelim): 10
length of query: 537
length of database: 12,070,560
effective HSP length: 85
effective length of query: 452
effective length of database: 9,609,640
effective search space: 4343557280
effective search space used: 4343557280
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.7 bits)
S2: 62 (29.1 bits)

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