Silkworm BLAST Result against mosquito

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000934-TA|BGIBMGA000934-PA|IPR001148|Carbonic anhydrase,
eukaryotic
         (257 letters)

Database: mosquito 
           2123 sequences; 516,269 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY280612-1|AAQ21365.1|  309|Anopheles gambiae carbonic anhydrase...    66   1e-12
DQ518576-1|ABF66618.1|  276|Anopheles gambiae putative cytoplasm...    62   1e-11
AY280613-1|AAQ21366.1|  257|Anopheles gambiae carbonic anhydrase...    58   3e-10
DQ518577-1|ABF66619.1|  318|Anopheles gambiae putative secreted ...    46   1e-06
AB090813-2|BAC57902.1| 1099|Anopheles gambiae reverse transcript...    27   0.71 
AF203335-1|AAF19830.1|  175|Anopheles gambiae immune-responsive ...    25   1.6  
AY255857-1|AAP13483.1|  216|Anopheles gambiae glutathione tranfe...    25   2.8  
AB090817-2|BAC57910.1| 1009|Anopheles gambiae reverse transcript...    23   6.6  

>AY280612-1|AAQ21365.1|  309|Anopheles gambiae carbonic anhydrase
           protein.
          Length = 309

 Score = 65.7 bits (153), Expect = 1e-12
 Identities = 73/244 (29%), Positives = 112/244 (45%), Gaps = 43/244 (17%)

Query: 20  CNKGRRQSPVNLEPEKLL---FDPNLRFLHIDKHRINGLISNTGHSVIFTVENETRHHIN 76
           C+ GRRQSP++L     +   F P L F +         ++NTGHS+     N     I 
Sbjct: 44  CDNGRRQSPIDLTIAAAVRGQFAP-LFFSNYMLPLKQPRVTNTGHSIQI---NNRDSAIT 99

Query: 77  ITGGPLSYKYQFHEIHIH-----------YGL------HD-QFGS-EHAINGYSFPAELG 117
           + GG L  ++   ++H H           YGL      HD ++ S E A+   +  A LG
Sbjct: 100 MQGGGLGGRFVLDQMHFHWGSEHTLDDTRYGLELHLVHHDTRYASLEDAVQARNGVAVLG 159

Query: 118 DL----SNPELRVLT-----EELENIKYGGAEMPVNKLSVRGLLPD--TDYYMTYDGSTT 166
            L    S P + + T      E++N + G   +   KLS   LLP   T +Y  Y+GS T
Sbjct: 160 VLFHVGSQPNMHIDTILDTATEIQN-EVGKEALLRGKLSPYNLLPSNRTSFYR-YEGSLT 217

Query: 167 APACFETVTWIIVNKPIYITKQQLHGLRRLMQGDAKHPKAPLGNNFRPPQPLHHRAVRTN 226
            PAC E+V W +  + I ++ +Q+   + +     +     L NNFR  QPL+ RA+   
Sbjct: 218 TPACAESVIWTVFTESISVSLEQVERFKAIHDQTGRE----LVNNFRSVQPLNTRALVYA 273

Query: 227 IDFD 230
            ++D
Sbjct: 274 TEWD 277


>DQ518576-1|ABF66618.1|  276|Anopheles gambiae putative cytoplasmic
           carbonic anhydrase protein.
          Length = 276

 Score = 62.5 bits (145), Expect = 1e-11
 Identities = 41/117 (35%), Positives = 59/117 (50%), Gaps = 13/117 (11%)

Query: 121 NPELRVLTEELENIKYGGAEMPVNK-LSVRGLLPDTDYYMTYDGSTTAPACFETVTWIIV 179
           +PEL ++   L  I + G  + +NK L    LLP+   Y TY GS T P C E+VTWI+ 
Sbjct: 155 HPELDIIARLLPFITHKGDRVTLNKPLDPARLLPEGKAYWTYLGSLTTPPCSESVTWILF 214

Query: 180 NKPIYITKQQLHGLRRLMQGDA------------KHPKAPLGNNFRPPQPLHHRAVR 224
            +PI ++ +QL   R +   DA            +     + NNFRPP  L +R +R
Sbjct: 215 KEPIEVSHEQLELFREMRCYDAAEECPCDETLNKQFDYGKVINNFRPPLELGNRQLR 271



 Score = 46.4 bits (105), Expect = 8e-07
 Identities = 39/118 (33%), Positives = 58/118 (49%), Gaps = 17/118 (14%)

Query: 6   GPAFWGLINPEWSLCNKGRRQSPVNLEPEKL-----LFDPNLRFLHIDKHRINGLISNTG 60
           GP  W    PE     +G+RQSPV++   K      L +  LR+ ++ ++  +  + N G
Sbjct: 12  GPQKW----PEMFPQARGQRQSPVDIVTSKTQNSGDLQENPLRWTYVPENTRS--LVNPG 65

Query: 61  HSVIFTVENETRHHINITGGPLSYKYQF--HEIHIHYGLHDQFGSEHAINGYSFPAEL 116
           +     V  +      +TGGPL  K QF   + H H+G  D  GSEH ++G SF  EL
Sbjct: 66  YCWRVDVNGKGSM---LTGGPLQ-KEQFILEQFHCHWGCSDSRGSEHTVDGESFAGEL 119


>AY280613-1|AAQ21366.1|  257|Anopheles gambiae carbonic anhydrase
           alternate isoform protein.
          Length = 257

 Score = 57.6 bits (133), Expect = 3e-10
 Identities = 41/141 (29%), Positives = 67/141 (47%), Gaps = 9/141 (6%)

Query: 83  SYKYQFHEIHIH--YGLHDQFGSEH---AINGYSFPAELGDLSNPELRVLTEELENIKYG 137
           +Y  + H +H +  YG   +   +    A+ G+ F    G+   P    +   L+ I+  
Sbjct: 97  TYSMEAHAVHYNAKYGSFAEAVDKPDGLAVLGF-FVQAYGNDDCPAFDKIVAGLQYIRSP 155

Query: 138 GAEMPVNK--LSVRGLLPDTDYYMTYDGSTTAPACFETVTWIIVNKPIYITKQQLHGLRR 195
            A+  ++   L+  G+     +Y TY GS T P  FE+VTW++   PIY++ +QL   R+
Sbjct: 156 DAQTEIDADCLAWMGMQELNRHYYTYKGSLTTPPYFESVTWLVYKTPIYVSSKQLEAFRQ 215

Query: 196 LMQGDAKHPKAPLGNNFRPPQ 216
           L Q   K     + NNFR  Q
Sbjct: 216 L-QACPKDSSKKIVNNFRSVQ 235



 Score = 44.8 bits (101), Expect = 2e-06
 Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 1/58 (1%)

Query: 58  NTGHSVIFTVENETRHHINITGGPLSYKYQFHEIHIHYGLHDQFGSEHAINGYSFPAE 115
           NTG S + T  +       I GG L  KY F ++H H+G+ D  G EH + G ++  E
Sbjct: 45  NTGSSAMITFSDRPFRPF-IVGGVLGNKYIFEQLHFHWGIGDGSGCEHTLEGSTYSME 101


>DQ518577-1|ABF66619.1|  318|Anopheles gambiae putative secreted
           carbonic anhydrase protein.
          Length = 318

 Score = 46.0 bits (104), Expect = 1e-06
 Identities = 27/100 (27%), Positives = 48/100 (48%), Gaps = 6/100 (6%)

Query: 23  GRRQSPVNLEPEKL--LFDPNLRFLHIDKHRINGL-ISNTGHSVIFTV---ENETRHHIN 76
           G  QSP+ +   +   L+ P +  +  +      + I N GHSV  ++   +  +  H  
Sbjct: 54  GAHQSPIAIHSHRAVPLYMPAIELVGYNNLLPGPMTIHNNGHSVSLSIPKTDPTSGKHPY 113

Query: 77  ITGGPLSYKYQFHEIHIHYGLHDQFGSEHAINGYSFPAEL 116
           I GG L  +Y+   +H H+G  +  G+EH +N   +P E+
Sbjct: 114 ILGGKLENEYELEGLHFHWGDKNNRGAEHVLNDIRYPLEM 153



 Score = 45.6 bits (103), Expect = 1e-06
 Identities = 34/148 (22%), Positives = 63/148 (42%), Gaps = 9/148 (6%)

Query: 82  LSYKYQFHEIHI---HYGLHDQFGSEHAINGYSFPAELGDLSNPELRVLTEELENIKYGG 138
           + Y  + H IH    +  + +  G    +    F  ++ +   P +  L      I    
Sbjct: 147 IRYPLEMHIIHRNKKYKSVGEALGYSDGLTVLGFFYQVTEQDAPSINTLVRSFGQIVDYD 206

Query: 139 AEMPVNK-LSVRGLLPDTDY--YMTYDGSTTAPACFETVTWIIVNKPIYITKQQLHGLRR 195
             + +N   +++ L+   D   + TY GS T P C E VTW++    + ++  Q+   R 
Sbjct: 207 QTLQLNHTFTLQSLIDGIDLTRFYTYKGSLTTPPCSEAVTWVVFPDLLELSVNQMKRFRT 266

Query: 196 LMQGDAKHPKAPLGNNFRPPQPLHHRAV 223
           L   D     +P+ +N+R  QP+ +R V
Sbjct: 267 L---DTGIHGSPMVDNYRALQPIGNRRV 291


>AB090813-2|BAC57902.1| 1099|Anopheles gambiae reverse transcriptase
           protein.
          Length = 1099

 Score = 26.6 bits (56), Expect = 0.71
 Identities = 19/80 (23%), Positives = 35/80 (43%), Gaps = 4/80 (5%)

Query: 107 INGYSFPAELGDLSNPELRVLTEELENIKYGGAEMPVNKLSVRGLLPDTDYYMT-YDG-- 163
           I G   P  + D+S+ EL+++   + N K  G +   N      +L  T  +   Y    
Sbjct: 401 IEGEEQPGAVADVSDDELKLIARRMANKKAPGLDGIPNAAVKAAILEHTGVFTALYQDCL 460

Query: 164 -STTAPACFETVTWIIVNKP 182
            + T PA ++    +++ KP
Sbjct: 461 VNGTFPAAWKRQRLVLIPKP 480


>AF203335-1|AAF19830.1|  175|Anopheles gambiae immune-responsive
           serine protease-relatedprotein ISPR20 protein.
          Length = 175

 Score = 25.4 bits (53), Expect = 1.6
 Identities = 10/25 (40%), Positives = 14/25 (56%)

Query: 45  LHIDKHRINGLISNTGHSVIFTVEN 69
           +HI  + I G      H +IFT+EN
Sbjct: 106 VHIPPYEIEGCGHRNPHGMIFTIEN 130


>AY255857-1|AAP13483.1|  216|Anopheles gambiae glutathione
           tranferase d9 protein.
          Length = 216

 Score = 24.6 bits (51), Expect = 2.8
 Identities = 10/29 (34%), Positives = 13/29 (44%)

Query: 206 APLGNNFRPPQPLHHRAVRTNIDFDLNKL 234
           AP G  + PP PL    V   + F+   L
Sbjct: 77  APAGTTYYPPDPLRRAIVNQRLLFECGTL 105


>AB090817-2|BAC57910.1| 1009|Anopheles gambiae reverse transcriptase
           protein.
          Length = 1009

 Score = 23.4 bits (48), Expect = 6.6
 Identities = 15/52 (28%), Positives = 23/52 (44%), Gaps = 1/52 (1%)

Query: 113 PAELGDLSNPELRVLTEELENIKYGGAEMPVNKLSVRGLLPDTD-YYMTYDG 163
           P E   ++N E+  +   L N K  G +   N+    G+L  TD +   Y G
Sbjct: 391 PREFERITNDEILAVARRLPNKKAPGPDGIPNEALKVGMLTATDAFCRVYQG 442


  Database: mosquito
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 516,269
  Number of sequences in database:  2123
  
Lambda     K      H
   0.319    0.139    0.433 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 314,604
Number of Sequences: 2123
Number of extensions: 14006
Number of successful extensions: 29
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 14
Number of HSP's gapped (non-prelim): 12
length of query: 257
length of database: 516,269
effective HSP length: 63
effective length of query: 194
effective length of database: 382,520
effective search space: 74208880
effective search space used: 74208880
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 47 (23.0 bits)