BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000934-TA|BGIBMGA000934-PA|IPR001148|Carbonic anhydrase, eukaryotic (257 letters) Database: mosquito 2123 sequences; 516,269 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AY280612-1|AAQ21365.1| 309|Anopheles gambiae carbonic anhydrase... 66 1e-12 DQ518576-1|ABF66618.1| 276|Anopheles gambiae putative cytoplasm... 62 1e-11 AY280613-1|AAQ21366.1| 257|Anopheles gambiae carbonic anhydrase... 58 3e-10 DQ518577-1|ABF66619.1| 318|Anopheles gambiae putative secreted ... 46 1e-06 AB090813-2|BAC57902.1| 1099|Anopheles gambiae reverse transcript... 27 0.71 AF203335-1|AAF19830.1| 175|Anopheles gambiae immune-responsive ... 25 1.6 AY255857-1|AAP13483.1| 216|Anopheles gambiae glutathione tranfe... 25 2.8 AB090817-2|BAC57910.1| 1009|Anopheles gambiae reverse transcript... 23 6.6 >AY280612-1|AAQ21365.1| 309|Anopheles gambiae carbonic anhydrase protein. Length = 309 Score = 65.7 bits (153), Expect = 1e-12 Identities = 73/244 (29%), Positives = 112/244 (45%), Gaps = 43/244 (17%) Query: 20 CNKGRRQSPVNLEPEKLL---FDPNLRFLHIDKHRINGLISNTGHSVIFTVENETRHHIN 76 C+ GRRQSP++L + F P L F + ++NTGHS+ N I Sbjct: 44 CDNGRRQSPIDLTIAAAVRGQFAP-LFFSNYMLPLKQPRVTNTGHSIQI---NNRDSAIT 99 Query: 77 ITGGPLSYKYQFHEIHIH-----------YGL------HD-QFGS-EHAINGYSFPAELG 117 + GG L ++ ++H H YGL HD ++ S E A+ + A LG Sbjct: 100 MQGGGLGGRFVLDQMHFHWGSEHTLDDTRYGLELHLVHHDTRYASLEDAVQARNGVAVLG 159 Query: 118 DL----SNPELRVLT-----EELENIKYGGAEMPVNKLSVRGLLPD--TDYYMTYDGSTT 166 L S P + + T E++N + G + KLS LLP T +Y Y+GS T Sbjct: 160 VLFHVGSQPNMHIDTILDTATEIQN-EVGKEALLRGKLSPYNLLPSNRTSFYR-YEGSLT 217 Query: 167 APACFETVTWIIVNKPIYITKQQLHGLRRLMQGDAKHPKAPLGNNFRPPQPLHHRAVRTN 226 PAC E+V W + + I ++ +Q+ + + + L NNFR QPL+ RA+ Sbjct: 218 TPACAESVIWTVFTESISVSLEQVERFKAIHDQTGRE----LVNNFRSVQPLNTRALVYA 273 Query: 227 IDFD 230 ++D Sbjct: 274 TEWD 277 >DQ518576-1|ABF66618.1| 276|Anopheles gambiae putative cytoplasmic carbonic anhydrase protein. Length = 276 Score = 62.5 bits (145), Expect = 1e-11 Identities = 41/117 (35%), Positives = 59/117 (50%), Gaps = 13/117 (11%) Query: 121 NPELRVLTEELENIKYGGAEMPVNK-LSVRGLLPDTDYYMTYDGSTTAPACFETVTWIIV 179 +PEL ++ L I + G + +NK L LLP+ Y TY GS T P C E+VTWI+ Sbjct: 155 HPELDIIARLLPFITHKGDRVTLNKPLDPARLLPEGKAYWTYLGSLTTPPCSESVTWILF 214 Query: 180 NKPIYITKQQLHGLRRLMQGDA------------KHPKAPLGNNFRPPQPLHHRAVR 224 +PI ++ +QL R + DA + + NNFRPP L +R +R Sbjct: 215 KEPIEVSHEQLELFREMRCYDAAEECPCDETLNKQFDYGKVINNFRPPLELGNRQLR 271 Score = 46.4 bits (105), Expect = 8e-07 Identities = 39/118 (33%), Positives = 58/118 (49%), Gaps = 17/118 (14%) Query: 6 GPAFWGLINPEWSLCNKGRRQSPVNLEPEKL-----LFDPNLRFLHIDKHRINGLISNTG 60 GP W PE +G+RQSPV++ K L + LR+ ++ ++ + + N G Sbjct: 12 GPQKW----PEMFPQARGQRQSPVDIVTSKTQNSGDLQENPLRWTYVPENTRS--LVNPG 65 Query: 61 HSVIFTVENETRHHINITGGPLSYKYQF--HEIHIHYGLHDQFGSEHAINGYSFPAEL 116 + V + +TGGPL K QF + H H+G D GSEH ++G SF EL Sbjct: 66 YCWRVDVNGKGSM---LTGGPLQ-KEQFILEQFHCHWGCSDSRGSEHTVDGESFAGEL 119 >AY280613-1|AAQ21366.1| 257|Anopheles gambiae carbonic anhydrase alternate isoform protein. Length = 257 Score = 57.6 bits (133), Expect = 3e-10 Identities = 41/141 (29%), Positives = 67/141 (47%), Gaps = 9/141 (6%) Query: 83 SYKYQFHEIHIH--YGLHDQFGSEH---AINGYSFPAELGDLSNPELRVLTEELENIKYG 137 +Y + H +H + YG + + A+ G+ F G+ P + L+ I+ Sbjct: 97 TYSMEAHAVHYNAKYGSFAEAVDKPDGLAVLGF-FVQAYGNDDCPAFDKIVAGLQYIRSP 155 Query: 138 GAEMPVNK--LSVRGLLPDTDYYMTYDGSTTAPACFETVTWIIVNKPIYITKQQLHGLRR 195 A+ ++ L+ G+ +Y TY GS T P FE+VTW++ PIY++ +QL R+ Sbjct: 156 DAQTEIDADCLAWMGMQELNRHYYTYKGSLTTPPYFESVTWLVYKTPIYVSSKQLEAFRQ 215 Query: 196 LMQGDAKHPKAPLGNNFRPPQ 216 L Q K + NNFR Q Sbjct: 216 L-QACPKDSSKKIVNNFRSVQ 235 Score = 44.8 bits (101), Expect = 2e-06 Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 1/58 (1%) Query: 58 NTGHSVIFTVENETRHHINITGGPLSYKYQFHEIHIHYGLHDQFGSEHAINGYSFPAE 115 NTG S + T + I GG L KY F ++H H+G+ D G EH + G ++ E Sbjct: 45 NTGSSAMITFSDRPFRPF-IVGGVLGNKYIFEQLHFHWGIGDGSGCEHTLEGSTYSME 101 >DQ518577-1|ABF66619.1| 318|Anopheles gambiae putative secreted carbonic anhydrase protein. Length = 318 Score = 46.0 bits (104), Expect = 1e-06 Identities = 27/100 (27%), Positives = 48/100 (48%), Gaps = 6/100 (6%) Query: 23 GRRQSPVNLEPEKL--LFDPNLRFLHIDKHRINGL-ISNTGHSVIFTV---ENETRHHIN 76 G QSP+ + + L+ P + + + + I N GHSV ++ + + H Sbjct: 54 GAHQSPIAIHSHRAVPLYMPAIELVGYNNLLPGPMTIHNNGHSVSLSIPKTDPTSGKHPY 113 Query: 77 ITGGPLSYKYQFHEIHIHYGLHDQFGSEHAINGYSFPAEL 116 I GG L +Y+ +H H+G + G+EH +N +P E+ Sbjct: 114 ILGGKLENEYELEGLHFHWGDKNNRGAEHVLNDIRYPLEM 153 Score = 45.6 bits (103), Expect = 1e-06 Identities = 34/148 (22%), Positives = 63/148 (42%), Gaps = 9/148 (6%) Query: 82 LSYKYQFHEIHI---HYGLHDQFGSEHAINGYSFPAELGDLSNPELRVLTEELENIKYGG 138 + Y + H IH + + + G + F ++ + P + L I Sbjct: 147 IRYPLEMHIIHRNKKYKSVGEALGYSDGLTVLGFFYQVTEQDAPSINTLVRSFGQIVDYD 206 Query: 139 AEMPVNK-LSVRGLLPDTDY--YMTYDGSTTAPACFETVTWIIVNKPIYITKQQLHGLRR 195 + +N +++ L+ D + TY GS T P C E VTW++ + ++ Q+ R Sbjct: 207 QTLQLNHTFTLQSLIDGIDLTRFYTYKGSLTTPPCSEAVTWVVFPDLLELSVNQMKRFRT 266 Query: 196 LMQGDAKHPKAPLGNNFRPPQPLHHRAV 223 L D +P+ +N+R QP+ +R V Sbjct: 267 L---DTGIHGSPMVDNYRALQPIGNRRV 291 >AB090813-2|BAC57902.1| 1099|Anopheles gambiae reverse transcriptase protein. Length = 1099 Score = 26.6 bits (56), Expect = 0.71 Identities = 19/80 (23%), Positives = 35/80 (43%), Gaps = 4/80 (5%) Query: 107 INGYSFPAELGDLSNPELRVLTEELENIKYGGAEMPVNKLSVRGLLPDTDYYMT-YDG-- 163 I G P + D+S+ EL+++ + N K G + N +L T + Y Sbjct: 401 IEGEEQPGAVADVSDDELKLIARRMANKKAPGLDGIPNAAVKAAILEHTGVFTALYQDCL 460 Query: 164 -STTAPACFETVTWIIVNKP 182 + T PA ++ +++ KP Sbjct: 461 VNGTFPAAWKRQRLVLIPKP 480 >AF203335-1|AAF19830.1| 175|Anopheles gambiae immune-responsive serine protease-relatedprotein ISPR20 protein. Length = 175 Score = 25.4 bits (53), Expect = 1.6 Identities = 10/25 (40%), Positives = 14/25 (56%) Query: 45 LHIDKHRINGLISNTGHSVIFTVEN 69 +HI + I G H +IFT+EN Sbjct: 106 VHIPPYEIEGCGHRNPHGMIFTIEN 130 >AY255857-1|AAP13483.1| 216|Anopheles gambiae glutathione tranferase d9 protein. Length = 216 Score = 24.6 bits (51), Expect = 2.8 Identities = 10/29 (34%), Positives = 13/29 (44%) Query: 206 APLGNNFRPPQPLHHRAVRTNIDFDLNKL 234 AP G + PP PL V + F+ L Sbjct: 77 APAGTTYYPPDPLRRAIVNQRLLFECGTL 105 >AB090817-2|BAC57910.1| 1009|Anopheles gambiae reverse transcriptase protein. Length = 1009 Score = 23.4 bits (48), Expect = 6.6 Identities = 15/52 (28%), Positives = 23/52 (44%), Gaps = 1/52 (1%) Query: 113 PAELGDLSNPELRVLTEELENIKYGGAEMPVNKLSVRGLLPDTD-YYMTYDG 163 P E ++N E+ + L N K G + N+ G+L TD + Y G Sbjct: 391 PREFERITNDEILAVARRLPNKKAPGPDGIPNEALKVGMLTATDAFCRVYQG 442 Database: mosquito Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 516,269 Number of sequences in database: 2123 Lambda K H 0.319 0.139 0.433 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 314,604 Number of Sequences: 2123 Number of extensions: 14006 Number of successful extensions: 29 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 14 Number of HSP's gapped (non-prelim): 12 length of query: 257 length of database: 516,269 effective HSP length: 63 effective length of query: 194 effective length of database: 382,520 effective search space: 74208880 effective search space used: 74208880 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 47 (23.0 bits)
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